Job ID = 16435530 SRX = SRX11633398 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:24:14 63145710 reads; of these: 63145710 (100.00%) were unpaired; of these: 5254140 (8.32%) aligned 0 times 49859033 (78.96%) aligned exactly 1 time 8032537 (12.72%) aligned >1 times 91.68% overall alignment rate Time searching: 00:24:15 Overall time: 00:24:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 9937583 / 57891570 = 0.1717 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:41:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:41:08: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:41:08: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:41:18: 1000000 INFO @ Tue, 02 Aug 2022 11:41:28: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:41:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:41:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:41:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:41:39: 3000000 INFO @ Tue, 02 Aug 2022 11:41:47: 1000000 INFO @ Tue, 02 Aug 2022 11:41:49: 4000000 INFO @ Tue, 02 Aug 2022 11:41:58: 2000000 INFO @ Tue, 02 Aug 2022 11:42:00: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:42:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:42:07: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:42:07: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:42:09: 3000000 INFO @ Tue, 02 Aug 2022 11:42:12: 6000000 INFO @ Tue, 02 Aug 2022 11:42:18: 1000000 INFO @ Tue, 02 Aug 2022 11:42:20: 4000000 INFO @ Tue, 02 Aug 2022 11:42:23: 7000000 INFO @ Tue, 02 Aug 2022 11:42:29: 2000000 INFO @ Tue, 02 Aug 2022 11:42:30: 5000000 INFO @ Tue, 02 Aug 2022 11:42:34: 8000000 INFO @ Tue, 02 Aug 2022 11:42:40: 3000000 INFO @ Tue, 02 Aug 2022 11:42:41: 6000000 INFO @ Tue, 02 Aug 2022 11:42:45: 9000000 INFO @ Tue, 02 Aug 2022 11:42:51: 4000000 INFO @ Tue, 02 Aug 2022 11:42:52: 7000000 INFO @ Tue, 02 Aug 2022 11:42:56: 10000000 INFO @ Tue, 02 Aug 2022 11:43:02: 5000000 INFO @ Tue, 02 Aug 2022 11:43:03: 8000000 INFO @ Tue, 02 Aug 2022 11:43:08: 11000000 INFO @ Tue, 02 Aug 2022 11:43:13: 6000000 INFO @ Tue, 02 Aug 2022 11:43:14: 9000000 INFO @ Tue, 02 Aug 2022 11:43:19: 12000000 INFO @ Tue, 02 Aug 2022 11:43:24: 7000000 INFO @ Tue, 02 Aug 2022 11:43:25: 10000000 INFO @ Tue, 02 Aug 2022 11:43:30: 13000000 INFO @ Tue, 02 Aug 2022 11:43:35: 8000000 INFO @ Tue, 02 Aug 2022 11:43:36: 11000000 INFO @ Tue, 02 Aug 2022 11:43:41: 14000000 INFO @ Tue, 02 Aug 2022 11:43:46: 9000000 INFO @ Tue, 02 Aug 2022 11:43:47: 12000000 INFO @ Tue, 02 Aug 2022 11:43:51: 15000000 INFO @ Tue, 02 Aug 2022 11:43:57: 10000000 INFO @ Tue, 02 Aug 2022 11:43:58: 13000000 INFO @ Tue, 02 Aug 2022 11:44:02: 16000000 INFO @ Tue, 02 Aug 2022 11:44:08: 11000000 INFO @ Tue, 02 Aug 2022 11:44:09: 14000000 INFO @ Tue, 02 Aug 2022 11:44:13: 17000000 INFO @ Tue, 02 Aug 2022 11:44:19: 12000000 INFO @ Tue, 02 Aug 2022 11:44:20: 15000000 INFO @ Tue, 02 Aug 2022 11:44:24: 18000000 INFO @ Tue, 02 Aug 2022 11:44:30: 13000000 INFO @ Tue, 02 Aug 2022 11:44:31: 16000000 INFO @ Tue, 02 Aug 2022 11:44:35: 19000000 INFO @ Tue, 02 Aug 2022 11:44:41: 14000000 INFO @ Tue, 02 Aug 2022 11:44:42: 17000000 INFO @ Tue, 02 Aug 2022 11:44:46: 20000000 INFO @ Tue, 02 Aug 2022 11:44:52: 15000000 INFO @ Tue, 02 Aug 2022 11:44:52: 18000000 INFO @ Tue, 02 Aug 2022 11:44:57: 21000000 INFO @ Tue, 02 Aug 2022 11:45:03: 16000000 INFO @ Tue, 02 Aug 2022 11:45:03: 19000000 INFO @ Tue, 02 Aug 2022 11:45:08: 22000000 INFO @ Tue, 02 Aug 2022 11:45:14: 17000000 INFO @ Tue, 02 Aug 2022 11:45:14: 20000000 INFO @ Tue, 02 Aug 2022 11:45:19: 23000000 INFO @ Tue, 02 Aug 2022 11:45:25: 18000000 INFO @ Tue, 02 Aug 2022 11:45:25: 21000000 INFO @ Tue, 02 Aug 2022 11:45:30: 24000000 INFO @ Tue, 02 Aug 2022 11:45:35: 19000000 INFO @ Tue, 02 Aug 2022 11:45:36: 22000000 INFO @ Tue, 02 Aug 2022 11:45:41: 25000000 INFO @ Tue, 02 Aug 2022 11:45:46: 20000000 INFO @ Tue, 02 Aug 2022 11:45:46: 23000000 INFO @ Tue, 02 Aug 2022 11:45:52: 26000000 INFO @ Tue, 02 Aug 2022 11:45:57: 24000000 INFO @ Tue, 02 Aug 2022 11:45:57: 21000000 INFO @ Tue, 02 Aug 2022 11:46:02: 27000000 INFO @ Tue, 02 Aug 2022 11:46:08: 25000000 INFO @ Tue, 02 Aug 2022 11:46:08: 22000000 INFO @ Tue, 02 Aug 2022 11:46:13: 28000000 INFO @ Tue, 02 Aug 2022 11:46:19: 26000000 INFO @ Tue, 02 Aug 2022 11:46:19: 23000000 INFO @ Tue, 02 Aug 2022 11:46:24: 29000000 INFO @ Tue, 02 Aug 2022 11:46:30: 27000000 INFO @ Tue, 02 Aug 2022 11:46:30: 24000000 INFO @ Tue, 02 Aug 2022 11:46:35: 30000000 INFO @ Tue, 02 Aug 2022 11:46:41: 25000000 INFO @ Tue, 02 Aug 2022 11:46:42: 28000000 INFO @ Tue, 02 Aug 2022 11:46:46: 31000000 INFO @ Tue, 02 Aug 2022 11:46:52: 26000000 INFO @ Tue, 02 Aug 2022 11:46:53: 29000000 INFO @ Tue, 02 Aug 2022 11:46:57: 32000000 INFO @ Tue, 02 Aug 2022 11:47:03: 27000000 INFO @ Tue, 02 Aug 2022 11:47:03: 30000000 INFO @ Tue, 02 Aug 2022 11:47:08: 33000000 INFO @ Tue, 02 Aug 2022 11:47:14: 28000000 INFO @ Tue, 02 Aug 2022 11:47:14: 31000000 INFO @ Tue, 02 Aug 2022 11:47:19: 34000000 INFO @ Tue, 02 Aug 2022 11:47:25: 29000000 INFO @ Tue, 02 Aug 2022 11:47:25: 32000000 INFO @ Tue, 02 Aug 2022 11:47:30: 35000000 INFO @ Tue, 02 Aug 2022 11:47:35: 30000000 INFO @ Tue, 02 Aug 2022 11:47:36: 33000000 INFO @ Tue, 02 Aug 2022 11:47:40: 36000000 INFO @ Tue, 02 Aug 2022 11:47:46: 31000000 INFO @ Tue, 02 Aug 2022 11:47:47: 34000000 INFO @ Tue, 02 Aug 2022 11:47:51: 37000000 INFO @ Tue, 02 Aug 2022 11:47:57: 32000000 INFO @ Tue, 02 Aug 2022 11:47:57: 35000000 INFO @ Tue, 02 Aug 2022 11:48:02: 38000000 INFO @ Tue, 02 Aug 2022 11:48:08: 33000000 INFO @ Tue, 02 Aug 2022 11:48:08: 36000000 INFO @ Tue, 02 Aug 2022 11:48:13: 39000000 INFO @ Tue, 02 Aug 2022 11:48:19: 34000000 INFO @ Tue, 02 Aug 2022 11:48:19: 37000000 INFO @ Tue, 02 Aug 2022 11:48:24: 40000000 INFO @ Tue, 02 Aug 2022 11:48:29: 35000000 INFO @ Tue, 02 Aug 2022 11:48:30: 38000000 INFO @ Tue, 02 Aug 2022 11:48:35: 41000000 INFO @ Tue, 02 Aug 2022 11:48:40: 36000000 INFO @ Tue, 02 Aug 2022 11:48:41: 39000000 INFO @ Tue, 02 Aug 2022 11:48:46: 42000000 INFO @ Tue, 02 Aug 2022 11:48:51: 37000000 INFO @ Tue, 02 Aug 2022 11:48:51: 40000000 INFO @ Tue, 02 Aug 2022 11:48:57: 43000000 INFO @ Tue, 02 Aug 2022 11:49:02: 38000000 INFO @ Tue, 02 Aug 2022 11:49:02: 41000000 INFO @ Tue, 02 Aug 2022 11:49:07: 44000000 INFO @ Tue, 02 Aug 2022 11:49:13: 42000000 INFO @ Tue, 02 Aug 2022 11:49:13: 39000000 INFO @ Tue, 02 Aug 2022 11:49:18: 45000000 INFO @ Tue, 02 Aug 2022 11:49:23: 43000000 INFO @ Tue, 02 Aug 2022 11:49:23: 40000000 INFO @ Tue, 02 Aug 2022 11:49:29: 46000000 INFO @ Tue, 02 Aug 2022 11:49:34: 44000000 INFO @ Tue, 02 Aug 2022 11:49:34: 41000000 INFO @ Tue, 02 Aug 2022 11:49:40: 47000000 INFO @ Tue, 02 Aug 2022 11:49:45: 45000000 INFO @ Tue, 02 Aug 2022 11:49:45: 42000000 INFO @ Tue, 02 Aug 2022 11:49:50: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:49:50: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:49:50: #1 total tags in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:49:50: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:49:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:49:51: #1 tags after filtering in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:49:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:49:51: #1 finished! INFO @ Tue, 02 Aug 2022 11:49:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:49:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:49:54: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:49:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:49:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:49:55: 43000000 INFO @ Tue, 02 Aug 2022 11:49:56: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 11:50:05: 44000000 INFO @ Tue, 02 Aug 2022 11:50:06: 47000000 INFO @ Tue, 02 Aug 2022 11:50:16: 45000000 INFO @ Tue, 02 Aug 2022 11:50:17: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:50:17: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:50:17: #1 total tags in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:50:17: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:50:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:50:18: #1 tags after filtering in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:50:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:50:18: #1 finished! INFO @ Tue, 02 Aug 2022 11:50:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:50:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:50:21: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:50:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:50:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:50:26: 46000000 INFO @ Tue, 02 Aug 2022 11:50:37: 47000000 INFO @ Tue, 02 Aug 2022 11:50:47: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:50:47: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:50:47: #1 total tags in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:50:47: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:50:48: #1 tags after filtering in treatment: 47953987 INFO @ Tue, 02 Aug 2022 11:50:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:50:48: #1 finished! INFO @ Tue, 02 Aug 2022 11:50:48: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:50:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:50:51: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:50:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:50:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633398/SRX11633398.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。