Job ID = 16435253 SRX = SRX11633389 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:25:17 71465335 reads; of these: 71465335 (100.00%) were unpaired; of these: 6341467 (8.87%) aligned 0 times 56098639 (78.50%) aligned exactly 1 time 9025229 (12.63%) aligned >1 times 91.13% overall alignment rate Time searching: 00:25:17 Overall time: 00:25:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 12025183 / 65123868 = 0.1847 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:33:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:33:36: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:33:36: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:33:44: 1000000 INFO @ Tue, 02 Aug 2022 11:33:52: 2000000 INFO @ Tue, 02 Aug 2022 11:34:00: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:34:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:34:05: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:34:05: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:34:09: 4000000 INFO @ Tue, 02 Aug 2022 11:34:13: 1000000 INFO @ Tue, 02 Aug 2022 11:34:18: 5000000 INFO @ Tue, 02 Aug 2022 11:34:22: 2000000 INFO @ Tue, 02 Aug 2022 11:34:27: 6000000 INFO @ Tue, 02 Aug 2022 11:34:30: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 11:34:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 11:34:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 11:34:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 11:34:35: 7000000 INFO @ Tue, 02 Aug 2022 11:34:38: 4000000 INFO @ Tue, 02 Aug 2022 11:34:43: 1000000 INFO @ Tue, 02 Aug 2022 11:34:44: 8000000 INFO @ Tue, 02 Aug 2022 11:34:47: 5000000 INFO @ Tue, 02 Aug 2022 11:34:52: 2000000 INFO @ Tue, 02 Aug 2022 11:34:53: 9000000 INFO @ Tue, 02 Aug 2022 11:34:56: 6000000 INFO @ Tue, 02 Aug 2022 11:35:00: 3000000 INFO @ Tue, 02 Aug 2022 11:35:02: 10000000 INFO @ Tue, 02 Aug 2022 11:35:04: 7000000 INFO @ Tue, 02 Aug 2022 11:35:09: 4000000 INFO @ Tue, 02 Aug 2022 11:35:11: 11000000 INFO @ Tue, 02 Aug 2022 11:35:13: 8000000 INFO @ Tue, 02 Aug 2022 11:35:17: 5000000 INFO @ Tue, 02 Aug 2022 11:35:20: 12000000 INFO @ Tue, 02 Aug 2022 11:35:21: 9000000 INFO @ Tue, 02 Aug 2022 11:35:25: 6000000 INFO @ Tue, 02 Aug 2022 11:35:28: 13000000 INFO @ Tue, 02 Aug 2022 11:35:30: 10000000 INFO @ Tue, 02 Aug 2022 11:35:34: 7000000 INFO @ Tue, 02 Aug 2022 11:35:37: 14000000 INFO @ Tue, 02 Aug 2022 11:35:38: 11000000 INFO @ Tue, 02 Aug 2022 11:35:43: 8000000 INFO @ Tue, 02 Aug 2022 11:35:45: 15000000 INFO @ Tue, 02 Aug 2022 11:35:47: 12000000 INFO @ Tue, 02 Aug 2022 11:35:51: 9000000 INFO @ Tue, 02 Aug 2022 11:35:54: 16000000 INFO @ Tue, 02 Aug 2022 11:35:56: 13000000 INFO @ Tue, 02 Aug 2022 11:36:00: 10000000 INFO @ Tue, 02 Aug 2022 11:36:02: 17000000 INFO @ Tue, 02 Aug 2022 11:36:05: 14000000 INFO @ Tue, 02 Aug 2022 11:36:08: 11000000 INFO @ Tue, 02 Aug 2022 11:36:11: 18000000 INFO @ Tue, 02 Aug 2022 11:36:13: 15000000 INFO @ Tue, 02 Aug 2022 11:36:17: 12000000 INFO @ Tue, 02 Aug 2022 11:36:19: 19000000 INFO @ Tue, 02 Aug 2022 11:36:22: 16000000 INFO @ Tue, 02 Aug 2022 11:36:26: 13000000 INFO @ Tue, 02 Aug 2022 11:36:28: 20000000 INFO @ Tue, 02 Aug 2022 11:36:31: 17000000 INFO @ Tue, 02 Aug 2022 11:36:35: 14000000 INFO @ Tue, 02 Aug 2022 11:36:36: 21000000 INFO @ Tue, 02 Aug 2022 11:36:40: 18000000 INFO @ Tue, 02 Aug 2022 11:36:44: 15000000 INFO @ Tue, 02 Aug 2022 11:36:45: 22000000 INFO @ Tue, 02 Aug 2022 11:36:49: 19000000 INFO @ Tue, 02 Aug 2022 11:36:53: 16000000 INFO @ Tue, 02 Aug 2022 11:36:53: 23000000 INFO @ Tue, 02 Aug 2022 11:36:58: 20000000 INFO @ Tue, 02 Aug 2022 11:37:02: 24000000 INFO @ Tue, 02 Aug 2022 11:37:02: 17000000 INFO @ Tue, 02 Aug 2022 11:37:07: 21000000 INFO @ Tue, 02 Aug 2022 11:37:10: 25000000 INFO @ Tue, 02 Aug 2022 11:37:11: 18000000 INFO @ Tue, 02 Aug 2022 11:37:17: 22000000 INFO @ Tue, 02 Aug 2022 11:37:20: 26000000 INFO @ Tue, 02 Aug 2022 11:37:21: 19000000 INFO @ Tue, 02 Aug 2022 11:37:27: 23000000 INFO @ Tue, 02 Aug 2022 11:37:29: 27000000 INFO @ Tue, 02 Aug 2022 11:37:31: 20000000 INFO @ Tue, 02 Aug 2022 11:37:36: 24000000 INFO @ Tue, 02 Aug 2022 11:37:39: 28000000 INFO @ Tue, 02 Aug 2022 11:37:41: 21000000 INFO @ Tue, 02 Aug 2022 11:37:46: 25000000 INFO @ Tue, 02 Aug 2022 11:37:48: 29000000 INFO @ Tue, 02 Aug 2022 11:37:51: 22000000 INFO @ Tue, 02 Aug 2022 11:37:56: 26000000 INFO @ Tue, 02 Aug 2022 11:37:58: 30000000 INFO @ Tue, 02 Aug 2022 11:38:00: 23000000 INFO @ Tue, 02 Aug 2022 11:38:05: 27000000 INFO @ Tue, 02 Aug 2022 11:38:07: 31000000 INFO @ Tue, 02 Aug 2022 11:38:09: 24000000 INFO @ Tue, 02 Aug 2022 11:38:14: 28000000 INFO @ Tue, 02 Aug 2022 11:38:17: 32000000 INFO @ Tue, 02 Aug 2022 11:38:17: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 11:38:23: 29000000 INFO @ Tue, 02 Aug 2022 11:38:26: 26000000 INFO @ Tue, 02 Aug 2022 11:38:28: 33000000 INFO @ Tue, 02 Aug 2022 11:38:33: 30000000 INFO @ Tue, 02 Aug 2022 11:38:36: 27000000 INFO @ Tue, 02 Aug 2022 11:38:39: 34000000 INFO @ Tue, 02 Aug 2022 11:38:42: 31000000 INFO @ Tue, 02 Aug 2022 11:38:45: 28000000 INFO @ Tue, 02 Aug 2022 11:38:49: 35000000 INFO @ Tue, 02 Aug 2022 11:38:51: 32000000 INFO @ Tue, 02 Aug 2022 11:38:54: 29000000 INFO @ Tue, 02 Aug 2022 11:38:59: 36000000 INFO @ Tue, 02 Aug 2022 11:39:01: 33000000 INFO @ Tue, 02 Aug 2022 11:39:04: 30000000 INFO @ Tue, 02 Aug 2022 11:39:08: 37000000 INFO @ Tue, 02 Aug 2022 11:39:10: 34000000 INFO @ Tue, 02 Aug 2022 11:39:13: 31000000 INFO @ Tue, 02 Aug 2022 11:39:18: 38000000 INFO @ Tue, 02 Aug 2022 11:39:19: 35000000 INFO @ Tue, 02 Aug 2022 11:39:22: 32000000 INFO @ Tue, 02 Aug 2022 11:39:28: 39000000 INFO @ Tue, 02 Aug 2022 11:39:28: 36000000 INFO @ Tue, 02 Aug 2022 11:39:31: 33000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 11:39:37: 37000000 INFO @ Tue, 02 Aug 2022 11:39:38: 40000000 INFO @ Tue, 02 Aug 2022 11:39:40: 34000000 INFO @ Tue, 02 Aug 2022 11:39:46: 38000000 INFO @ Tue, 02 Aug 2022 11:39:47: 41000000 INFO @ Tue, 02 Aug 2022 11:39:49: 35000000 INFO @ Tue, 02 Aug 2022 11:39:55: 39000000 INFO @ Tue, 02 Aug 2022 11:39:57: 42000000 INFO @ Tue, 02 Aug 2022 11:39:58: 36000000 INFO @ Tue, 02 Aug 2022 11:40:04: 40000000 INFO @ Tue, 02 Aug 2022 11:40:06: 43000000 INFO @ Tue, 02 Aug 2022 11:40:08: 37000000 INFO @ Tue, 02 Aug 2022 11:40:13: 41000000 INFO @ Tue, 02 Aug 2022 11:40:15: 44000000 INFO @ Tue, 02 Aug 2022 11:40:16: 38000000 INFO @ Tue, 02 Aug 2022 11:40:22: 42000000 INFO @ Tue, 02 Aug 2022 11:40:25: 45000000 INFO @ Tue, 02 Aug 2022 11:40:25: 39000000 INFO @ Tue, 02 Aug 2022 11:40:30: 43000000 INFO @ Tue, 02 Aug 2022 11:40:34: 40000000 INFO @ Tue, 02 Aug 2022 11:40:34: 46000000 INFO @ Tue, 02 Aug 2022 11:40:38: 44000000 INFO @ Tue, 02 Aug 2022 11:40:43: 41000000 INFO @ Tue, 02 Aug 2022 11:40:44: 47000000 INFO @ Tue, 02 Aug 2022 11:40:47: 45000000 INFO @ Tue, 02 Aug 2022 11:40:51: 42000000 INFO @ Tue, 02 Aug 2022 11:40:53: 48000000 INFO @ Tue, 02 Aug 2022 11:40:56: 46000000 INFO @ Tue, 02 Aug 2022 11:41:00: 43000000 INFO @ Tue, 02 Aug 2022 11:41:02: 49000000 INFO @ Tue, 02 Aug 2022 11:41:05: 47000000 INFO @ Tue, 02 Aug 2022 11:41:09: 44000000 INFO @ Tue, 02 Aug 2022 11:41:12: 50000000 INFO @ Tue, 02 Aug 2022 11:41:13: 48000000 INFO @ Tue, 02 Aug 2022 11:41:18: 45000000 INFO @ Tue, 02 Aug 2022 11:41:21: 51000000 INFO @ Tue, 02 Aug 2022 11:41:22: 49000000 INFO @ Tue, 02 Aug 2022 11:41:26: 46000000 INFO @ Tue, 02 Aug 2022 11:41:31: 50000000 INFO @ Tue, 02 Aug 2022 11:41:31: 52000000 INFO @ Tue, 02 Aug 2022 11:41:35: 47000000 INFO @ Tue, 02 Aug 2022 11:41:39: 51000000 INFO @ Tue, 02 Aug 2022 11:41:40: 53000000 INFO @ Tue, 02 Aug 2022 11:41:42: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:41:42: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:41:42: #1 total tags in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:41:42: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:41:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:41:42: #1 tags after filtering in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:41:42: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:41:42: #1 finished! INFO @ Tue, 02 Aug 2022 11:41:42: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:41:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:41:44: 48000000 INFO @ Tue, 02 Aug 2022 11:41:46: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:41:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:41:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:41:48: 52000000 INFO @ Tue, 02 Aug 2022 11:41:52: 49000000 INFO @ Tue, 02 Aug 2022 11:41:56: 53000000 INFO @ Tue, 02 Aug 2022 11:41:57: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:41:57: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:41:57: #1 total tags in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:41:57: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:41:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:41:58: #1 tags after filtering in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:41:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:41:58: #1 finished! INFO @ Tue, 02 Aug 2022 11:41:58: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:41:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:42:00: 50000000 INFO @ Tue, 02 Aug 2022 11:42:01: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:42:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:42:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 11:42:07: 51000000 INFO @ Tue, 02 Aug 2022 11:42:14: 52000000 INFO @ Tue, 02 Aug 2022 11:42:21: 53000000 INFO @ Tue, 02 Aug 2022 11:42:22: #1 tag size is determined as 74 bps INFO @ Tue, 02 Aug 2022 11:42:22: #1 tag size = 74 INFO @ Tue, 02 Aug 2022 11:42:22: #1 total tags in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:42:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 11:42:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 11:42:23: #1 tags after filtering in treatment: 53098685 INFO @ Tue, 02 Aug 2022 11:42:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 11:42:23: #1 finished! INFO @ Tue, 02 Aug 2022 11:42:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 11:42:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 11:42:26: #2 number of paired peaks: 0 WARNING @ Tue, 02 Aug 2022 11:42:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 11:42:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX11633389/SRX11633389.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling