Job ID = 6366325 SRX = SRX1132921 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:33:21 prefetch.2.10.7: 1) Downloading 'SRR2144370'... 2020-06-15T22:33:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:35:40 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:35:40 prefetch.2.10.7: 1) 'SRR2144370' was downloaded successfully Read 32584830 spots for SRR2144370/SRR2144370.sra Written 32584830 spots for SRR2144370/SRR2144370.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:36 32584830 reads; of these: 32584830 (100.00%) were unpaired; of these: 1504453 (4.62%) aligned 0 times 26212668 (80.44%) aligned exactly 1 time 4867709 (14.94%) aligned >1 times 95.38% overall alignment rate Time searching: 00:10:36 Overall time: 00:10:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10909162 / 31080377 = 0.3510 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:58:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:58:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:58:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:58:42: 1000000 INFO @ Tue, 16 Jun 2020 07:58:48: 2000000 INFO @ Tue, 16 Jun 2020 07:58:55: 3000000 INFO @ Tue, 16 Jun 2020 07:59:01: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:05: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:05: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:08: 5000000 INFO @ Tue, 16 Jun 2020 07:59:12: 1000000 INFO @ Tue, 16 Jun 2020 07:59:14: 6000000 INFO @ Tue, 16 Jun 2020 07:59:18: 2000000 INFO @ Tue, 16 Jun 2020 07:59:21: 7000000 INFO @ Tue, 16 Jun 2020 07:59:24: 3000000 INFO @ Tue, 16 Jun 2020 07:59:28: 8000000 INFO @ Tue, 16 Jun 2020 07:59:30: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:34: 9000000 INFO @ Tue, 16 Jun 2020 07:59:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:35: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:35: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:37: 5000000 INFO @ Tue, 16 Jun 2020 07:59:42: 10000000 INFO @ Tue, 16 Jun 2020 07:59:43: 6000000 INFO @ Tue, 16 Jun 2020 07:59:43: 1000000 INFO @ Tue, 16 Jun 2020 07:59:49: 7000000 INFO @ Tue, 16 Jun 2020 07:59:50: 11000000 INFO @ Tue, 16 Jun 2020 07:59:51: 2000000 INFO @ Tue, 16 Jun 2020 07:59:56: 8000000 INFO @ Tue, 16 Jun 2020 07:59:58: 12000000 INFO @ Tue, 16 Jun 2020 07:59:59: 3000000 INFO @ Tue, 16 Jun 2020 08:00:02: 9000000 INFO @ Tue, 16 Jun 2020 08:00:06: 13000000 INFO @ Tue, 16 Jun 2020 08:00:07: 4000000 INFO @ Tue, 16 Jun 2020 08:00:09: 10000000 INFO @ Tue, 16 Jun 2020 08:00:13: 14000000 INFO @ Tue, 16 Jun 2020 08:00:15: 5000000 INFO @ Tue, 16 Jun 2020 08:00:15: 11000000 INFO @ Tue, 16 Jun 2020 08:00:21: 15000000 INFO @ Tue, 16 Jun 2020 08:00:21: 12000000 INFO @ Tue, 16 Jun 2020 08:00:23: 6000000 INFO @ Tue, 16 Jun 2020 08:00:28: 13000000 INFO @ Tue, 16 Jun 2020 08:00:29: 16000000 INFO @ Tue, 16 Jun 2020 08:00:31: 7000000 INFO @ Tue, 16 Jun 2020 08:00:34: 14000000 INFO @ Tue, 16 Jun 2020 08:00:36: 17000000 INFO @ Tue, 16 Jun 2020 08:00:38: 8000000 INFO @ Tue, 16 Jun 2020 08:00:40: 15000000 INFO @ Tue, 16 Jun 2020 08:00:44: 18000000 INFO @ Tue, 16 Jun 2020 08:00:46: 9000000 INFO @ Tue, 16 Jun 2020 08:00:47: 16000000 INFO @ Tue, 16 Jun 2020 08:00:52: 19000000 INFO @ Tue, 16 Jun 2020 08:00:53: 17000000 INFO @ Tue, 16 Jun 2020 08:00:54: 10000000 INFO @ Tue, 16 Jun 2020 08:00:59: 20000000 INFO @ Tue, 16 Jun 2020 08:00:59: 18000000 INFO @ Tue, 16 Jun 2020 08:01:00: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:01:00: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:01:00: #1 total tags in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:00: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:01:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:01:01: #1 tags after filtering in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:01:01: #1 finished! INFO @ Tue, 16 Jun 2020 08:01:01: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:01:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:01:01: 11000000 INFO @ Tue, 16 Jun 2020 08:01:02: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 08:01:02: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:01:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:01:05: 19000000 INFO @ Tue, 16 Jun 2020 08:01:08: 12000000 INFO @ Tue, 16 Jun 2020 08:01:12: 20000000 INFO @ Tue, 16 Jun 2020 08:01:13: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:01:13: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:01:13: #1 total tags in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:13: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:01:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:01:13: #1 tags after filtering in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:01:13: #1 finished! INFO @ Tue, 16 Jun 2020 08:01:13: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:01:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:01:15: 13000000 INFO @ Tue, 16 Jun 2020 08:01:15: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 08:01:15: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:01:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:01:21: 14000000 INFO @ Tue, 16 Jun 2020 08:01:27: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:01:33: 16000000 INFO @ Tue, 16 Jun 2020 08:01:39: 17000000 INFO @ Tue, 16 Jun 2020 08:01:45: 18000000 INFO @ Tue, 16 Jun 2020 08:01:51: 19000000 INFO @ Tue, 16 Jun 2020 08:01:57: 20000000 INFO @ Tue, 16 Jun 2020 08:01:58: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:01:58: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:01:58: #1 total tags in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:58: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:01:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:01:59: #1 tags after filtering in treatment: 20171215 INFO @ Tue, 16 Jun 2020 08:01:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:01:59: #1 finished! INFO @ Tue, 16 Jun 2020 08:01:59: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:01:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:02:00: #2 number of paired peaks: 8 WARNING @ Tue, 16 Jun 2020 08:02:00: Too few paired peaks (8) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:02:00: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132921/SRX1132921.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。