Job ID = 6366317 SRX = SRX1132913 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:34:20 prefetch.2.10.7: 1) Downloading 'SRR2144362'... 2020-06-15T23:34:20 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:38:21 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:38:21 prefetch.2.10.7: 1) 'SRR2144362' was downloaded successfully Read 22648545 spots for SRR2144362/SRR2144362.sra Written 22648545 spots for SRR2144362/SRR2144362.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:07:39 22648545 reads; of these: 22648545 (100.00%) were unpaired; of these: 840836 (3.71%) aligned 0 times 18501575 (81.69%) aligned exactly 1 time 3306134 (14.60%) aligned >1 times 96.29% overall alignment rate Time searching: 00:07:39 Overall time: 00:07:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 6681470 / 21807709 = 0.3064 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:54:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:54:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:54:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:55:03: 1000000 INFO @ Tue, 16 Jun 2020 08:55:10: 2000000 INFO @ Tue, 16 Jun 2020 08:55:18: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:55:25: 4000000 INFO @ Tue, 16 Jun 2020 08:55:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:55:26: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:55:26: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:55:32: 5000000 INFO @ Tue, 16 Jun 2020 08:55:34: 1000000 INFO @ Tue, 16 Jun 2020 08:55:39: 6000000 INFO @ Tue, 16 Jun 2020 08:55:41: 2000000 INFO @ Tue, 16 Jun 2020 08:55:46: 7000000 INFO @ Tue, 16 Jun 2020 08:55:48: 3000000 INFO @ Tue, 16 Jun 2020 08:55:54: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:55:56: 4000000 INFO @ Tue, 16 Jun 2020 08:55:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:55:56: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:55:56: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:56:01: 9000000 INFO @ Tue, 16 Jun 2020 08:56:03: 5000000 INFO @ Tue, 16 Jun 2020 08:56:04: 1000000 INFO @ Tue, 16 Jun 2020 08:56:09: 10000000 INFO @ Tue, 16 Jun 2020 08:56:11: 6000000 INFO @ Tue, 16 Jun 2020 08:56:11: 2000000 INFO @ Tue, 16 Jun 2020 08:56:16: 11000000 INFO @ Tue, 16 Jun 2020 08:56:19: 3000000 INFO @ Tue, 16 Jun 2020 08:56:19: 7000000 INFO @ Tue, 16 Jun 2020 08:56:23: 12000000 INFO @ Tue, 16 Jun 2020 08:56:26: 4000000 INFO @ Tue, 16 Jun 2020 08:56:26: 8000000 INFO @ Tue, 16 Jun 2020 08:56:31: 13000000 INFO @ Tue, 16 Jun 2020 08:56:34: 5000000 INFO @ Tue, 16 Jun 2020 08:56:34: 9000000 INFO @ Tue, 16 Jun 2020 08:56:38: 14000000 INFO @ Tue, 16 Jun 2020 08:56:41: 10000000 INFO @ Tue, 16 Jun 2020 08:56:41: 6000000 INFO @ Tue, 16 Jun 2020 08:56:46: 15000000 INFO @ Tue, 16 Jun 2020 08:56:47: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:56:47: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:56:47: #1 total tags in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:56:47: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:56:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:56:47: #1 tags after filtering in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:56:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:56:47: #1 finished! INFO @ Tue, 16 Jun 2020 08:56:47: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:56:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:56:48: #2 number of paired peaks: 19 WARNING @ Tue, 16 Jun 2020 08:56:48: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:56:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:56:48: 11000000 INFO @ Tue, 16 Jun 2020 08:56:48: 7000000 INFO @ Tue, 16 Jun 2020 08:56:56: 12000000 INFO @ Tue, 16 Jun 2020 08:56:56: 8000000 INFO @ Tue, 16 Jun 2020 08:57:03: 13000000 INFO @ Tue, 16 Jun 2020 08:57:03: 9000000 INFO @ Tue, 16 Jun 2020 08:57:10: 10000000 INFO @ Tue, 16 Jun 2020 08:57:10: 14000000 INFO @ Tue, 16 Jun 2020 08:57:17: 11000000 INFO @ Tue, 16 Jun 2020 08:57:17: 15000000 INFO @ Tue, 16 Jun 2020 08:57:18: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:57:18: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:57:18: #1 total tags in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:57:18: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:57:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:57:19: #1 tags after filtering in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:57:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:57:19: #1 finished! INFO @ Tue, 16 Jun 2020 08:57:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:57:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:57:19: #2 number of paired peaks: 19 WARNING @ Tue, 16 Jun 2020 08:57:19: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:57:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:57:24: 12000000 INFO @ Tue, 16 Jun 2020 08:57:30: 13000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:57:37: 14000000 INFO @ Tue, 16 Jun 2020 08:57:43: 15000000 INFO @ Tue, 16 Jun 2020 08:57:44: #1 tag size is determined as 50 bps INFO @ Tue, 16 Jun 2020 08:57:44: #1 tag size = 50 INFO @ Tue, 16 Jun 2020 08:57:44: #1 total tags in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:57:44: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:57:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:57:44: #1 tags after filtering in treatment: 15126239 INFO @ Tue, 16 Jun 2020 08:57:44: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:57:44: #1 finished! INFO @ Tue, 16 Jun 2020 08:57:44: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:57:44: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:57:45: #2 number of paired peaks: 19 WARNING @ Tue, 16 Jun 2020 08:57:45: Too few paired peaks (19) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:57:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1132913/SRX1132913.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。