Job ID = 6366292 SRX = SRX1078716 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:41:36 prefetch.2.10.7: 1) Downloading 'SRR2084323'... 2020-06-15T22:41:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:44:42 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:44:42 prefetch.2.10.7: 1) 'SRR2084323' was downloaded successfully Read 34725715 spots for SRR2084323/SRR2084323.sra Written 34725715 spots for SRR2084323/SRR2084323.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:08 34725715 reads; of these: 34725715 (100.00%) were unpaired; of these: 1110023 (3.20%) aligned 0 times 28206045 (81.23%) aligned exactly 1 time 5409647 (15.58%) aligned >1 times 96.80% overall alignment rate Time searching: 00:08:08 Overall time: 00:08:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5425425 / 33615692 = 0.1614 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:03:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:03:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:03:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:03:35: 1000000 INFO @ Tue, 16 Jun 2020 08:03:41: 2000000 INFO @ Tue, 16 Jun 2020 08:03:47: 3000000 INFO @ Tue, 16 Jun 2020 08:03:53: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:03:58: 5000000 INFO @ Tue, 16 Jun 2020 08:04:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:04:00: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:04:00: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:04:04: 6000000 INFO @ Tue, 16 Jun 2020 08:04:06: 1000000 INFO @ Tue, 16 Jun 2020 08:04:11: 7000000 INFO @ Tue, 16 Jun 2020 08:04:12: 2000000 INFO @ Tue, 16 Jun 2020 08:04:17: 8000000 INFO @ Tue, 16 Jun 2020 08:04:19: 3000000 INFO @ Tue, 16 Jun 2020 08:04:24: 9000000 INFO @ Tue, 16 Jun 2020 08:04:25: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:04:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:04:29: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:04:29: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:04:30: 10000000 INFO @ Tue, 16 Jun 2020 08:04:32: 5000000 INFO @ Tue, 16 Jun 2020 08:04:36: 1000000 INFO @ Tue, 16 Jun 2020 08:04:37: 11000000 INFO @ Tue, 16 Jun 2020 08:04:38: 6000000 INFO @ Tue, 16 Jun 2020 08:04:42: 2000000 INFO @ Tue, 16 Jun 2020 08:04:43: 12000000 INFO @ Tue, 16 Jun 2020 08:04:45: 7000000 INFO @ Tue, 16 Jun 2020 08:04:49: 3000000 INFO @ Tue, 16 Jun 2020 08:04:50: 13000000 INFO @ Tue, 16 Jun 2020 08:04:51: 8000000 INFO @ Tue, 16 Jun 2020 08:04:56: 4000000 INFO @ Tue, 16 Jun 2020 08:04:56: 14000000 INFO @ Tue, 16 Jun 2020 08:04:58: 9000000 INFO @ Tue, 16 Jun 2020 08:05:02: 5000000 INFO @ Tue, 16 Jun 2020 08:05:03: 15000000 INFO @ Tue, 16 Jun 2020 08:05:05: 10000000 INFO @ Tue, 16 Jun 2020 08:05:09: 6000000 INFO @ Tue, 16 Jun 2020 08:05:10: 16000000 INFO @ Tue, 16 Jun 2020 08:05:11: 11000000 INFO @ Tue, 16 Jun 2020 08:05:16: 7000000 INFO @ Tue, 16 Jun 2020 08:05:17: 17000000 INFO @ Tue, 16 Jun 2020 08:05:18: 12000000 INFO @ Tue, 16 Jun 2020 08:05:22: 8000000 INFO @ Tue, 16 Jun 2020 08:05:23: 18000000 INFO @ Tue, 16 Jun 2020 08:05:25: 13000000 INFO @ Tue, 16 Jun 2020 08:05:29: 9000000 INFO @ Tue, 16 Jun 2020 08:05:30: 19000000 INFO @ Tue, 16 Jun 2020 08:05:32: 14000000 INFO @ Tue, 16 Jun 2020 08:05:36: 10000000 INFO @ Tue, 16 Jun 2020 08:05:37: 20000000 INFO @ Tue, 16 Jun 2020 08:05:39: 15000000 INFO @ Tue, 16 Jun 2020 08:05:43: 11000000 INFO @ Tue, 16 Jun 2020 08:05:44: 21000000 INFO @ Tue, 16 Jun 2020 08:05:46: 16000000 INFO @ Tue, 16 Jun 2020 08:05:50: 12000000 INFO @ Tue, 16 Jun 2020 08:05:51: 22000000 INFO @ Tue, 16 Jun 2020 08:05:53: 17000000 INFO @ Tue, 16 Jun 2020 08:05:57: 13000000 INFO @ Tue, 16 Jun 2020 08:05:58: 23000000 INFO @ Tue, 16 Jun 2020 08:06:00: 18000000 INFO @ Tue, 16 Jun 2020 08:06:04: 14000000 INFO @ Tue, 16 Jun 2020 08:06:05: 24000000 INFO @ Tue, 16 Jun 2020 08:06:07: 19000000 INFO @ Tue, 16 Jun 2020 08:06:11: 15000000 INFO @ Tue, 16 Jun 2020 08:06:12: 25000000 INFO @ Tue, 16 Jun 2020 08:06:14: 20000000 INFO @ Tue, 16 Jun 2020 08:06:18: 16000000 INFO @ Tue, 16 Jun 2020 08:06:19: 26000000 INFO @ Tue, 16 Jun 2020 08:06:21: 21000000 INFO @ Tue, 16 Jun 2020 08:06:25: 17000000 INFO @ Tue, 16 Jun 2020 08:06:26: 27000000 INFO @ Tue, 16 Jun 2020 08:06:27: 22000000 INFO @ Tue, 16 Jun 2020 08:06:32: 18000000 INFO @ Tue, 16 Jun 2020 08:06:33: 28000000 INFO @ Tue, 16 Jun 2020 08:06:34: 23000000 INFO @ Tue, 16 Jun 2020 08:06:34: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:06:34: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:06:34: #1 total tags in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:06:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:06:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:06:35: #1 tags after filtering in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:06:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:06:35: #1 finished! INFO @ Tue, 16 Jun 2020 08:06:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:06:37: #2 number of paired peaks: 99 WARNING @ Tue, 16 Jun 2020 08:06:37: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:06:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:06:38: 19000000 INFO @ Tue, 16 Jun 2020 08:06:41: 24000000 INFO @ Tue, 16 Jun 2020 08:06:45: 20000000 INFO @ Tue, 16 Jun 2020 08:06:48: 25000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:06:52: 21000000 INFO @ Tue, 16 Jun 2020 08:06:55: 26000000 INFO @ Tue, 16 Jun 2020 08:06:59: 22000000 INFO @ Tue, 16 Jun 2020 08:07:02: 27000000 INFO @ Tue, 16 Jun 2020 08:07:06: 23000000 INFO @ Tue, 16 Jun 2020 08:07:09: 28000000 INFO @ Tue, 16 Jun 2020 08:07:10: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:07:10: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:07:10: #1 total tags in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:07:10: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:07:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:07:11: #1 tags after filtering in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:07:11: #1 finished! INFO @ Tue, 16 Jun 2020 08:07:11: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:07:13: #2 number of paired peaks: 99 WARNING @ Tue, 16 Jun 2020 08:07:13: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:07:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:07:13: 24000000 INFO @ Tue, 16 Jun 2020 08:07:19: 25000000 INFO @ Tue, 16 Jun 2020 08:07:25: 26000000 INFO @ Tue, 16 Jun 2020 08:07:31: 27000000 INFO @ Tue, 16 Jun 2020 08:07:37: 28000000 INFO @ Tue, 16 Jun 2020 08:07:38: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:07:38: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:07:38: #1 total tags in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:07:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:07:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:07:38: #1 tags after filtering in treatment: 28190267 INFO @ Tue, 16 Jun 2020 08:07:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:07:38: #1 finished! INFO @ Tue, 16 Jun 2020 08:07:38: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:07:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:07:40: #2 number of paired peaks: 99 WARNING @ Tue, 16 Jun 2020 08:07:40: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:07:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1078716/SRX1078716.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。