Job ID = 16434928 SRX = SRX10435518 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:36 34975676 reads; of these: 34975676 (100.00%) were unpaired; of these: 711410 (2.03%) aligned 0 times 28473859 (81.41%) aligned exactly 1 time 5790407 (16.56%) aligned >1 times 97.97% overall alignment rate Time searching: 00:11:36 Overall time: 00:11:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 5494120 / 34264266 = 0.1603 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:34:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:34:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:34:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:34:58: 1000000 INFO @ Tue, 02 Aug 2022 10:35:03: 2000000 INFO @ Tue, 02 Aug 2022 10:35:09: 3000000 INFO @ Tue, 02 Aug 2022 10:35:14: 4000000 INFO @ Tue, 02 Aug 2022 10:35:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:35:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:35:22: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:35:22: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:35:25: 6000000 INFO @ Tue, 02 Aug 2022 10:35:28: 1000000 INFO @ Tue, 02 Aug 2022 10:35:31: 7000000 INFO @ Tue, 02 Aug 2022 10:35:34: 2000000 INFO @ Tue, 02 Aug 2022 10:35:37: 8000000 INFO @ Tue, 02 Aug 2022 10:35:40: 3000000 INFO @ Tue, 02 Aug 2022 10:35:42: 9000000 INFO @ Tue, 02 Aug 2022 10:35:46: 4000000 INFO @ Tue, 02 Aug 2022 10:35:48: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:35:51: 5000000 INFO @ Tue, 02 Aug 2022 10:35:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:35:52: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:35:52: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:35:54: 11000000 INFO @ Tue, 02 Aug 2022 10:35:57: 6000000 INFO @ Tue, 02 Aug 2022 10:35:59: 1000000 INFO @ Tue, 02 Aug 2022 10:36:00: 12000000 INFO @ Tue, 02 Aug 2022 10:36:03: 7000000 INFO @ Tue, 02 Aug 2022 10:36:04: 2000000 INFO @ Tue, 02 Aug 2022 10:36:06: 13000000 INFO @ Tue, 02 Aug 2022 10:36:09: 8000000 INFO @ Tue, 02 Aug 2022 10:36:10: 3000000 INFO @ Tue, 02 Aug 2022 10:36:12: 14000000 INFO @ Tue, 02 Aug 2022 10:36:15: 9000000 INFO @ Tue, 02 Aug 2022 10:36:16: 4000000 INFO @ Tue, 02 Aug 2022 10:36:18: 15000000 INFO @ Tue, 02 Aug 2022 10:36:21: 10000000 INFO @ Tue, 02 Aug 2022 10:36:22: 5000000 INFO @ Tue, 02 Aug 2022 10:36:23: 16000000 INFO @ Tue, 02 Aug 2022 10:36:27: 11000000 INFO @ Tue, 02 Aug 2022 10:36:28: 6000000 INFO @ Tue, 02 Aug 2022 10:36:29: 17000000 INFO @ Tue, 02 Aug 2022 10:36:33: 12000000 INFO @ Tue, 02 Aug 2022 10:36:34: 7000000 INFO @ Tue, 02 Aug 2022 10:36:35: 18000000 INFO @ Tue, 02 Aug 2022 10:36:39: 13000000 INFO @ Tue, 02 Aug 2022 10:36:40: 8000000 INFO @ Tue, 02 Aug 2022 10:36:41: 19000000 INFO @ Tue, 02 Aug 2022 10:36:45: 14000000 INFO @ Tue, 02 Aug 2022 10:36:46: 9000000 INFO @ Tue, 02 Aug 2022 10:36:47: 20000000 INFO @ Tue, 02 Aug 2022 10:36:51: 15000000 INFO @ Tue, 02 Aug 2022 10:36:52: 10000000 INFO @ Tue, 02 Aug 2022 10:36:52: 21000000 INFO @ Tue, 02 Aug 2022 10:36:57: 16000000 INFO @ Tue, 02 Aug 2022 10:36:58: 11000000 INFO @ Tue, 02 Aug 2022 10:36:58: 22000000 INFO @ Tue, 02 Aug 2022 10:37:02: 17000000 INFO @ Tue, 02 Aug 2022 10:37:04: 12000000 INFO @ Tue, 02 Aug 2022 10:37:04: 23000000 INFO @ Tue, 02 Aug 2022 10:37:08: 18000000 INFO @ Tue, 02 Aug 2022 10:37:09: 13000000 INFO @ Tue, 02 Aug 2022 10:37:10: 24000000 INFO @ Tue, 02 Aug 2022 10:37:14: 19000000 INFO @ Tue, 02 Aug 2022 10:37:15: 14000000 INFO @ Tue, 02 Aug 2022 10:37:16: 25000000 INFO @ Tue, 02 Aug 2022 10:37:20: 20000000 INFO @ Tue, 02 Aug 2022 10:37:21: 15000000 INFO @ Tue, 02 Aug 2022 10:37:21: 26000000 INFO @ Tue, 02 Aug 2022 10:37:25: 21000000 INFO @ Tue, 02 Aug 2022 10:37:27: 16000000 INFO @ Tue, 02 Aug 2022 10:37:27: 27000000 INFO @ Tue, 02 Aug 2022 10:37:31: 22000000 INFO @ Tue, 02 Aug 2022 10:37:33: 17000000 INFO @ Tue, 02 Aug 2022 10:37:33: 28000000 INFO @ Tue, 02 Aug 2022 10:37:37: 23000000 INFO @ Tue, 02 Aug 2022 10:37:38: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:37:38: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:37:38: #1 total tags in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:37:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:37:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:37:38: #1 tags after filtering in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:37:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:37:38: #1 finished! INFO @ Tue, 02 Aug 2022 10:37:38: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:37:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:37:38: 18000000 INFO @ Tue, 02 Aug 2022 10:37:40: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 10:37:40: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:37:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:37:42: 24000000 INFO @ Tue, 02 Aug 2022 10:37:44: 19000000 INFO @ Tue, 02 Aug 2022 10:37:48: 25000000 INFO @ Tue, 02 Aug 2022 10:37:50: 20000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:37:54: 26000000 INFO @ Tue, 02 Aug 2022 10:37:56: 21000000 INFO @ Tue, 02 Aug 2022 10:38:00: 27000000 INFO @ Tue, 02 Aug 2022 10:38:01: 22000000 INFO @ Tue, 02 Aug 2022 10:38:05: 28000000 INFO @ Tue, 02 Aug 2022 10:38:07: 23000000 INFO @ Tue, 02 Aug 2022 10:38:10: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:38:10: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:38:10: #1 total tags in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:38:10: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:38:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:38:10: #1 tags after filtering in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:38:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:38:10: #1 finished! INFO @ Tue, 02 Aug 2022 10:38:10: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:38:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:38:12: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 10:38:12: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:38:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:38:13: 24000000 INFO @ Tue, 02 Aug 2022 10:38:18: 25000000 INFO @ Tue, 02 Aug 2022 10:38:24: 26000000 INFO @ Tue, 02 Aug 2022 10:38:29: 27000000 INFO @ Tue, 02 Aug 2022 10:38:34: 28000000 INFO @ Tue, 02 Aug 2022 10:38:38: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:38:38: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:38:38: #1 total tags in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:38:38: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:38:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:38:39: #1 tags after filtering in treatment: 28770146 INFO @ Tue, 02 Aug 2022 10:38:39: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:38:39: #1 finished! INFO @ Tue, 02 Aug 2022 10:38:39: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:38:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:38:40: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 10:38:40: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:38:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435518/SRX10435518.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。