Job ID = 16434901 SRX = SRX10435513 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:17 31057573 reads; of these: 31057573 (100.00%) were unpaired; of these: 1651206 (5.32%) aligned 0 times 25439515 (81.91%) aligned exactly 1 time 3966852 (12.77%) aligned >1 times 94.68% overall alignment rate Time searching: 00:10:17 Overall time: 00:10:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5381554 / 29406367 = 0.1830 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:29:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:29:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:29:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:29:51: 1000000 INFO @ Tue, 02 Aug 2022 10:29:56: 2000000 INFO @ Tue, 02 Aug 2022 10:30:01: 3000000 INFO @ Tue, 02 Aug 2022 10:30:06: 4000000 INFO @ Tue, 02 Aug 2022 10:30:11: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:30:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:30:15: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:30:15: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:30:17: 6000000 INFO @ Tue, 02 Aug 2022 10:30:22: 1000000 INFO @ Tue, 02 Aug 2022 10:30:22: 7000000 INFO @ Tue, 02 Aug 2022 10:30:28: 2000000 INFO @ Tue, 02 Aug 2022 10:30:28: 8000000 INFO @ Tue, 02 Aug 2022 10:30:33: 9000000 INFO @ Tue, 02 Aug 2022 10:30:34: 3000000 INFO @ Tue, 02 Aug 2022 10:30:39: 10000000 INFO @ Tue, 02 Aug 2022 10:30:39: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 10:30:45: 11000000 INFO @ Tue, 02 Aug 2022 10:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 10:30:45: #1 read tag files... INFO @ Tue, 02 Aug 2022 10:30:45: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 10:30:45: 5000000 INFO @ Tue, 02 Aug 2022 10:30:50: 12000000 INFO @ Tue, 02 Aug 2022 10:30:52: 6000000 INFO @ Tue, 02 Aug 2022 10:30:52: 1000000 INFO @ Tue, 02 Aug 2022 10:30:56: 13000000 INFO @ Tue, 02 Aug 2022 10:30:58: 2000000 INFO @ Tue, 02 Aug 2022 10:30:58: 7000000 INFO @ Tue, 02 Aug 2022 10:31:02: 14000000 INFO @ Tue, 02 Aug 2022 10:31:04: 3000000 INFO @ Tue, 02 Aug 2022 10:31:05: 8000000 INFO @ Tue, 02 Aug 2022 10:31:08: 15000000 INFO @ Tue, 02 Aug 2022 10:31:11: 4000000 INFO @ Tue, 02 Aug 2022 10:31:12: 9000000 INFO @ Tue, 02 Aug 2022 10:31:13: 16000000 INFO @ Tue, 02 Aug 2022 10:31:17: 5000000 INFO @ Tue, 02 Aug 2022 10:31:18: 10000000 INFO @ Tue, 02 Aug 2022 10:31:19: 17000000 INFO @ Tue, 02 Aug 2022 10:31:22: 6000000 INFO @ Tue, 02 Aug 2022 10:31:25: 18000000 INFO @ Tue, 02 Aug 2022 10:31:25: 11000000 INFO @ Tue, 02 Aug 2022 10:31:28: 7000000 INFO @ Tue, 02 Aug 2022 10:31:31: 19000000 INFO @ Tue, 02 Aug 2022 10:31:32: 12000000 INFO @ Tue, 02 Aug 2022 10:31:34: 8000000 INFO @ Tue, 02 Aug 2022 10:31:36: 20000000 INFO @ Tue, 02 Aug 2022 10:31:38: 13000000 INFO @ Tue, 02 Aug 2022 10:31:40: 9000000 INFO @ Tue, 02 Aug 2022 10:31:42: 21000000 INFO @ Tue, 02 Aug 2022 10:31:45: 14000000 INFO @ Tue, 02 Aug 2022 10:31:45: 10000000 INFO @ Tue, 02 Aug 2022 10:31:48: 22000000 INFO @ Tue, 02 Aug 2022 10:31:51: 11000000 INFO @ Tue, 02 Aug 2022 10:31:52: 15000000 INFO @ Tue, 02 Aug 2022 10:31:53: 23000000 INFO @ Tue, 02 Aug 2022 10:31:57: 12000000 INFO @ Tue, 02 Aug 2022 10:31:59: 16000000 INFO @ Tue, 02 Aug 2022 10:31:59: 24000000 INFO @ Tue, 02 Aug 2022 10:31:59: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:31:59: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:31:59: #1 total tags in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:31:59: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:31:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:31:59: #1 tags after filtering in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:31:59: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:31:59: #1 finished! INFO @ Tue, 02 Aug 2022 10:31:59: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:31:59: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:32:01: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:32:01: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:32:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:32:02: 13000000 INFO @ Tue, 02 Aug 2022 10:32:05: 17000000 INFO @ Tue, 02 Aug 2022 10:32:08: 14000000 INFO @ Tue, 02 Aug 2022 10:32:12: 18000000 INFO @ Tue, 02 Aug 2022 10:32:14: 15000000 INFO @ Tue, 02 Aug 2022 10:32:19: 19000000 INFO @ Tue, 02 Aug 2022 10:32:20: 16000000 INFO @ Tue, 02 Aug 2022 10:32:25: 20000000 INFO @ Tue, 02 Aug 2022 10:32:25: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 10:32:31: 18000000 INFO @ Tue, 02 Aug 2022 10:32:31: 21000000 INFO @ Tue, 02 Aug 2022 10:32:37: 19000000 INFO @ Tue, 02 Aug 2022 10:32:38: 22000000 INFO @ Tue, 02 Aug 2022 10:32:42: 20000000 INFO @ Tue, 02 Aug 2022 10:32:44: 23000000 INFO @ Tue, 02 Aug 2022 10:32:48: 21000000 INFO @ Tue, 02 Aug 2022 10:32:51: 24000000 INFO @ Tue, 02 Aug 2022 10:32:51: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:32:51: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:32:51: #1 total tags in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:32:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:32:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:32:51: #1 tags after filtering in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:32:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:32:51: #1 finished! INFO @ Tue, 02 Aug 2022 10:32:51: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:32:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:32:53: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:32:53: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:32:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 10:32:54: 22000000 INFO @ Tue, 02 Aug 2022 10:32:59: 23000000 INFO @ Tue, 02 Aug 2022 10:33:04: 24000000 INFO @ Tue, 02 Aug 2022 10:33:04: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 10:33:04: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 10:33:04: #1 total tags in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:33:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 10:33:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 10:33:05: #1 tags after filtering in treatment: 24024813 INFO @ Tue, 02 Aug 2022 10:33:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 10:33:05: #1 finished! INFO @ Tue, 02 Aug 2022 10:33:05: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 10:33:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 10:33:06: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 10:33:06: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 10:33:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10435513/SRX10435513.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。