Job ID = 14158888 SRX = SRX10342585 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:31:19 43548484 reads; of these: 43548484 (100.00%) were paired; of these: 6501938 (14.93%) aligned concordantly 0 times 32063665 (73.63%) aligned concordantly exactly 1 time 4982881 (11.44%) aligned concordantly >1 times ---- 6501938 pairs aligned concordantly 0 times; of these: 4953449 (76.18%) aligned discordantly 1 time ---- 1548489 pairs aligned 0 times concordantly or discordantly; of these: 3096978 mates make up the pairs; of these: 972804 (31.41%) aligned 0 times 870790 (28.12%) aligned exactly 1 time 1253384 (40.47%) aligned >1 times 98.88% overall alignment rate Time searching: 00:31:19 Overall time: 00:31:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4209403 / 41935725 = 0.1004 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:01:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:01:00: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:01:00: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:01:04: 1000000 INFO @ Wed, 08 Dec 2021 19:01:08: 2000000 INFO @ Wed, 08 Dec 2021 19:01:12: 3000000 INFO @ Wed, 08 Dec 2021 19:01:16: 4000000 INFO @ Wed, 08 Dec 2021 19:01:20: 5000000 INFO @ Wed, 08 Dec 2021 19:01:23: 6000000 INFO @ Wed, 08 Dec 2021 19:01:27: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:01:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:01:30: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:01:30: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:01:31: 8000000 INFO @ Wed, 08 Dec 2021 19:01:34: 1000000 INFO @ Wed, 08 Dec 2021 19:01:36: 9000000 INFO @ Wed, 08 Dec 2021 19:01:39: 2000000 INFO @ Wed, 08 Dec 2021 19:01:40: 10000000 INFO @ Wed, 08 Dec 2021 19:01:43: 3000000 INFO @ Wed, 08 Dec 2021 19:01:44: 11000000 INFO @ Wed, 08 Dec 2021 19:01:47: 4000000 INFO @ Wed, 08 Dec 2021 19:01:48: 12000000 INFO @ Wed, 08 Dec 2021 19:01:51: 5000000 INFO @ Wed, 08 Dec 2021 19:01:52: 13000000 INFO @ Wed, 08 Dec 2021 19:01:56: 6000000 INFO @ Wed, 08 Dec 2021 19:01:56: 14000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:01:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:01:59: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:01:59: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:02:00: 7000000 INFO @ Wed, 08 Dec 2021 19:02:01: 15000000 INFO @ Wed, 08 Dec 2021 19:02:04: 1000000 INFO @ Wed, 08 Dec 2021 19:02:04: 8000000 INFO @ Wed, 08 Dec 2021 19:02:05: 16000000 INFO @ Wed, 08 Dec 2021 19:02:08: 2000000 INFO @ Wed, 08 Dec 2021 19:02:08: 9000000 INFO @ Wed, 08 Dec 2021 19:02:09: 17000000 INFO @ Wed, 08 Dec 2021 19:02:12: 3000000 INFO @ Wed, 08 Dec 2021 19:02:12: 10000000 INFO @ Wed, 08 Dec 2021 19:02:13: 18000000 INFO @ Wed, 08 Dec 2021 19:02:16: 4000000 INFO @ Wed, 08 Dec 2021 19:02:16: 11000000 INFO @ Wed, 08 Dec 2021 19:02:17: 19000000 INFO @ Wed, 08 Dec 2021 19:02:20: 5000000 INFO @ Wed, 08 Dec 2021 19:02:21: 12000000 INFO @ Wed, 08 Dec 2021 19:02:21: 20000000 INFO @ Wed, 08 Dec 2021 19:02:25: 6000000 INFO @ Wed, 08 Dec 2021 19:02:25: 13000000 INFO @ Wed, 08 Dec 2021 19:02:25: 21000000 INFO @ Wed, 08 Dec 2021 19:02:29: 7000000 INFO @ Wed, 08 Dec 2021 19:02:29: 14000000 INFO @ Wed, 08 Dec 2021 19:02:30: 22000000 INFO @ Wed, 08 Dec 2021 19:02:33: 8000000 INFO @ Wed, 08 Dec 2021 19:02:33: 15000000 INFO @ Wed, 08 Dec 2021 19:02:34: 23000000 INFO @ Wed, 08 Dec 2021 19:02:37: 9000000 INFO @ Wed, 08 Dec 2021 19:02:37: 16000000 INFO @ Wed, 08 Dec 2021 19:02:38: 24000000 INFO @ Wed, 08 Dec 2021 19:02:41: 10000000 INFO @ Wed, 08 Dec 2021 19:02:41: 17000000 INFO @ Wed, 08 Dec 2021 19:02:42: 25000000 INFO @ Wed, 08 Dec 2021 19:02:45: 11000000 INFO @ Wed, 08 Dec 2021 19:02:45: 18000000 INFO @ Wed, 08 Dec 2021 19:02:46: 26000000 INFO @ Wed, 08 Dec 2021 19:02:50: 12000000 INFO @ Wed, 08 Dec 2021 19:02:50: 19000000 INFO @ Wed, 08 Dec 2021 19:02:50: 27000000 INFO @ Wed, 08 Dec 2021 19:02:54: 13000000 INFO @ Wed, 08 Dec 2021 19:02:54: 20000000 INFO @ Wed, 08 Dec 2021 19:02:54: 28000000 INFO @ Wed, 08 Dec 2021 19:02:58: 14000000 INFO @ Wed, 08 Dec 2021 19:02:58: 21000000 INFO @ Wed, 08 Dec 2021 19:02:59: 29000000 INFO @ Wed, 08 Dec 2021 19:03:02: 15000000 INFO @ Wed, 08 Dec 2021 19:03:02: 22000000 INFO @ Wed, 08 Dec 2021 19:03:03: 30000000 INFO @ Wed, 08 Dec 2021 19:03:06: 16000000 INFO @ Wed, 08 Dec 2021 19:03:07: 23000000 INFO @ Wed, 08 Dec 2021 19:03:07: 31000000 INFO @ Wed, 08 Dec 2021 19:03:10: 17000000 INFO @ Wed, 08 Dec 2021 19:03:11: 24000000 INFO @ Wed, 08 Dec 2021 19:03:11: 32000000 INFO @ Wed, 08 Dec 2021 19:03:14: 18000000 INFO @ Wed, 08 Dec 2021 19:03:15: 25000000 INFO @ Wed, 08 Dec 2021 19:03:15: 33000000 INFO @ Wed, 08 Dec 2021 19:03:19: 19000000 INFO @ Wed, 08 Dec 2021 19:03:19: 34000000 INFO @ Wed, 08 Dec 2021 19:03:19: 26000000 INFO @ Wed, 08 Dec 2021 19:03:23: 20000000 INFO @ Wed, 08 Dec 2021 19:03:23: 35000000 INFO @ Wed, 08 Dec 2021 19:03:24: 27000000 INFO @ Wed, 08 Dec 2021 19:03:27: 21000000 INFO @ Wed, 08 Dec 2021 19:03:27: 36000000 INFO @ Wed, 08 Dec 2021 19:03:28: 28000000 INFO @ Wed, 08 Dec 2021 19:03:31: 22000000 INFO @ Wed, 08 Dec 2021 19:03:32: 37000000 INFO @ Wed, 08 Dec 2021 19:03:32: 29000000 INFO @ Wed, 08 Dec 2021 19:03:35: 23000000 INFO @ Wed, 08 Dec 2021 19:03:36: 38000000 INFO @ Wed, 08 Dec 2021 19:03:36: 30000000 INFO @ Wed, 08 Dec 2021 19:03:39: 24000000 INFO @ Wed, 08 Dec 2021 19:03:40: 39000000 INFO @ Wed, 08 Dec 2021 19:03:40: 31000000 INFO @ Wed, 08 Dec 2021 19:03:43: 25000000 INFO @ Wed, 08 Dec 2021 19:03:44: 40000000 INFO @ Wed, 08 Dec 2021 19:03:44: 32000000 INFO @ Wed, 08 Dec 2021 19:03:48: 26000000 INFO @ Wed, 08 Dec 2021 19:03:48: 41000000 INFO @ Wed, 08 Dec 2021 19:03:48: 33000000 INFO @ Wed, 08 Dec 2021 19:03:52: 27000000 INFO @ Wed, 08 Dec 2021 19:03:52: 42000000 INFO @ Wed, 08 Dec 2021 19:03:52: 34000000 INFO @ Wed, 08 Dec 2021 19:03:56: 28000000 INFO @ Wed, 08 Dec 2021 19:03:56: 43000000 INFO @ Wed, 08 Dec 2021 19:03:57: 35000000 INFO @ Wed, 08 Dec 2021 19:04:00: 29000000 INFO @ Wed, 08 Dec 2021 19:04:01: 44000000 INFO @ Wed, 08 Dec 2021 19:04:01: 36000000 INFO @ Wed, 08 Dec 2021 19:04:04: 30000000 INFO @ Wed, 08 Dec 2021 19:04:05: 45000000 INFO @ Wed, 08 Dec 2021 19:04:05: 37000000 INFO @ Wed, 08 Dec 2021 19:04:08: 31000000 INFO @ Wed, 08 Dec 2021 19:04:09: 46000000 INFO @ Wed, 08 Dec 2021 19:04:09: 38000000 INFO @ Wed, 08 Dec 2021 19:04:12: 32000000 INFO @ Wed, 08 Dec 2021 19:04:13: 47000000 INFO @ Wed, 08 Dec 2021 19:04:13: 39000000 INFO @ Wed, 08 Dec 2021 19:04:17: 33000000 INFO @ Wed, 08 Dec 2021 19:04:17: 48000000 INFO @ Wed, 08 Dec 2021 19:04:17: 40000000 INFO @ Wed, 08 Dec 2021 19:04:21: 34000000 INFO @ Wed, 08 Dec 2021 19:04:21: 49000000 INFO @ Wed, 08 Dec 2021 19:04:22: 41000000 INFO @ Wed, 08 Dec 2021 19:04:25: 35000000 INFO @ Wed, 08 Dec 2021 19:04:25: 50000000 INFO @ Wed, 08 Dec 2021 19:04:26: 42000000 INFO @ Wed, 08 Dec 2021 19:04:29: 36000000 INFO @ Wed, 08 Dec 2021 19:04:29: 51000000 INFO @ Wed, 08 Dec 2021 19:04:30: 43000000 INFO @ Wed, 08 Dec 2021 19:04:33: 52000000 INFO @ Wed, 08 Dec 2021 19:04:33: 37000000 INFO @ Wed, 08 Dec 2021 19:04:34: 44000000 INFO @ Wed, 08 Dec 2021 19:04:37: 53000000 INFO @ Wed, 08 Dec 2021 19:04:37: 38000000 INFO @ Wed, 08 Dec 2021 19:04:38: 45000000 INFO @ Wed, 08 Dec 2021 19:04:41: 54000000 INFO @ Wed, 08 Dec 2021 19:04:42: 39000000 INFO @ Wed, 08 Dec 2021 19:04:42: 46000000 INFO @ Wed, 08 Dec 2021 19:04:45: 55000000 INFO @ Wed, 08 Dec 2021 19:04:46: 40000000 INFO @ Wed, 08 Dec 2021 19:04:46: 47000000 INFO @ Wed, 08 Dec 2021 19:04:50: 56000000 INFO @ Wed, 08 Dec 2021 19:04:50: 41000000 INFO @ Wed, 08 Dec 2021 19:04:50: 48000000 INFO @ Wed, 08 Dec 2021 19:04:54: 57000000 INFO @ Wed, 08 Dec 2021 19:04:54: 42000000 INFO @ Wed, 08 Dec 2021 19:04:54: 49000000 INFO @ Wed, 08 Dec 2021 19:04:58: 58000000 INFO @ Wed, 08 Dec 2021 19:04:58: 43000000 INFO @ Wed, 08 Dec 2021 19:04:59: 50000000 INFO @ Wed, 08 Dec 2021 19:05:02: 59000000 INFO @ Wed, 08 Dec 2021 19:05:02: 44000000 INFO @ Wed, 08 Dec 2021 19:05:03: 51000000 INFO @ Wed, 08 Dec 2021 19:05:06: 60000000 INFO @ Wed, 08 Dec 2021 19:05:06: 45000000 INFO @ Wed, 08 Dec 2021 19:05:07: 52000000 INFO @ Wed, 08 Dec 2021 19:05:10: 61000000 INFO @ Wed, 08 Dec 2021 19:05:10: 46000000 INFO @ Wed, 08 Dec 2021 19:05:11: 53000000 INFO @ Wed, 08 Dec 2021 19:05:14: 62000000 INFO @ Wed, 08 Dec 2021 19:05:14: 47000000 INFO @ Wed, 08 Dec 2021 19:05:15: 54000000 INFO @ Wed, 08 Dec 2021 19:05:18: 63000000 INFO @ Wed, 08 Dec 2021 19:05:19: 48000000 INFO @ Wed, 08 Dec 2021 19:05:19: 55000000 INFO @ Wed, 08 Dec 2021 19:05:22: 64000000 INFO @ Wed, 08 Dec 2021 19:05:23: 49000000 INFO @ Wed, 08 Dec 2021 19:05:23: 56000000 INFO @ Wed, 08 Dec 2021 19:05:26: 65000000 INFO @ Wed, 08 Dec 2021 19:05:27: 50000000 INFO @ Wed, 08 Dec 2021 19:05:27: 57000000 INFO @ Wed, 08 Dec 2021 19:05:30: 66000000 INFO @ Wed, 08 Dec 2021 19:05:31: 58000000 INFO @ Wed, 08 Dec 2021 19:05:31: 51000000 INFO @ Wed, 08 Dec 2021 19:05:34: 67000000 INFO @ Wed, 08 Dec 2021 19:05:35: 59000000 INFO @ Wed, 08 Dec 2021 19:05:35: 52000000 INFO @ Wed, 08 Dec 2021 19:05:38: 68000000 INFO @ Wed, 08 Dec 2021 19:05:39: 60000000 INFO @ Wed, 08 Dec 2021 19:05:39: 53000000 INFO @ Wed, 08 Dec 2021 19:05:42: 69000000 INFO @ Wed, 08 Dec 2021 19:05:43: 61000000 INFO @ Wed, 08 Dec 2021 19:05:43: 54000000 INFO @ Wed, 08 Dec 2021 19:05:46: 70000000 INFO @ Wed, 08 Dec 2021 19:05:47: 62000000 INFO @ Wed, 08 Dec 2021 19:05:47: 55000000 INFO @ Wed, 08 Dec 2021 19:05:50: 71000000 INFO @ Wed, 08 Dec 2021 19:05:51: 63000000 INFO @ Wed, 08 Dec 2021 19:05:51: 56000000 INFO @ Wed, 08 Dec 2021 19:05:54: 72000000 INFO @ Wed, 08 Dec 2021 19:05:55: 64000000 INFO @ Wed, 08 Dec 2021 19:05:55: 57000000 INFO @ Wed, 08 Dec 2021 19:05:58: 73000000 INFO @ Wed, 08 Dec 2021 19:05:59: 65000000 INFO @ Wed, 08 Dec 2021 19:05:59: 58000000 INFO @ Wed, 08 Dec 2021 19:06:03: 74000000 INFO @ Wed, 08 Dec 2021 19:06:03: 66000000 INFO @ Wed, 08 Dec 2021 19:06:03: 59000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 19:06:07: 75000000 INFO @ Wed, 08 Dec 2021 19:06:07: 67000000 INFO @ Wed, 08 Dec 2021 19:06:07: 60000000 INFO @ Wed, 08 Dec 2021 19:06:11: 76000000 INFO @ Wed, 08 Dec 2021 19:06:11: 68000000 INFO @ Wed, 08 Dec 2021 19:06:11: 61000000 INFO @ Wed, 08 Dec 2021 19:06:15: 77000000 INFO @ Wed, 08 Dec 2021 19:06:15: 69000000 INFO @ Wed, 08 Dec 2021 19:06:15: 62000000 INFO @ Wed, 08 Dec 2021 19:06:18: #1 tag size is determined as 38 bps INFO @ Wed, 08 Dec 2021 19:06:18: #1 tag size = 38 INFO @ Wed, 08 Dec 2021 19:06:18: #1 total tags in treatment: 33225175 INFO @ Wed, 08 Dec 2021 19:06:18: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:06:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:06:18: #1 tags after filtering in treatment: 28664814 INFO @ Wed, 08 Dec 2021 19:06:18: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 19:06:18: #1 finished! INFO @ Wed, 08 Dec 2021 19:06:18: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:06:18: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:06:19: 70000000 INFO @ Wed, 08 Dec 2021 19:06:19: 63000000 INFO @ Wed, 08 Dec 2021 19:06:20: #2 number of paired peaks: 145 WARNING @ Wed, 08 Dec 2021 19:06:20: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Wed, 08 Dec 2021 19:06:20: start model_add_line... INFO @ Wed, 08 Dec 2021 19:06:20: start X-correlation... INFO @ Wed, 08 Dec 2021 19:06:20: end of X-cor INFO @ Wed, 08 Dec 2021 19:06:20: #2 finished! INFO @ Wed, 08 Dec 2021 19:06:20: #2 predicted fragment length is 1 bps INFO @ Wed, 08 Dec 2021 19:06:20: #2 alternative fragment length(s) may be 1,36,125,568 bps INFO @ Wed, 08 Dec 2021 19:06:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05_model.r WARNING @ Wed, 08 Dec 2021 19:06:20: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 19:06:20: #2 You may need to consider one of the other alternative d(s): 1,36,125,568 WARNING @ Wed, 08 Dec 2021 19:06:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 19:06:20: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:06:20: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:06:23: 71000000 INFO @ Wed, 08 Dec 2021 19:06:23: 64000000 INFO @ Wed, 08 Dec 2021 19:06:27: 72000000 INFO @ Wed, 08 Dec 2021 19:06:28: 65000000 INFO @ Wed, 08 Dec 2021 19:06:31: 73000000 INFO @ Wed, 08 Dec 2021 19:06:32: 66000000 INFO @ Wed, 08 Dec 2021 19:06:35: 74000000 INFO @ Wed, 08 Dec 2021 19:06:36: 67000000 INFO @ Wed, 08 Dec 2021 19:06:39: 75000000 INFO @ Wed, 08 Dec 2021 19:06:40: 68000000 INFO @ Wed, 08 Dec 2021 19:06:43: 76000000 INFO @ Wed, 08 Dec 2021 19:06:44: 69000000 INFO @ Wed, 08 Dec 2021 19:06:47: 77000000 INFO @ Wed, 08 Dec 2021 19:06:48: 70000000 INFO @ Wed, 08 Dec 2021 19:06:50: #1 tag size is determined as 38 bps INFO @ Wed, 08 Dec 2021 19:06:50: #1 tag size = 38 INFO @ Wed, 08 Dec 2021 19:06:50: #1 total tags in treatment: 33225175 INFO @ Wed, 08 Dec 2021 19:06:50: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:06:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:06:51: #1 tags after filtering in treatment: 28664814 INFO @ Wed, 08 Dec 2021 19:06:51: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 19:06:51: #1 finished! INFO @ Wed, 08 Dec 2021 19:06:51: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:06:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:06:52: 71000000 INFO @ Wed, 08 Dec 2021 19:06:53: #2 number of paired peaks: 145 WARNING @ Wed, 08 Dec 2021 19:06:53: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Wed, 08 Dec 2021 19:06:53: start model_add_line... INFO @ Wed, 08 Dec 2021 19:06:53: start X-correlation... INFO @ Wed, 08 Dec 2021 19:06:53: end of X-cor INFO @ Wed, 08 Dec 2021 19:06:53: #2 finished! INFO @ Wed, 08 Dec 2021 19:06:53: #2 predicted fragment length is 1 bps INFO @ Wed, 08 Dec 2021 19:06:53: #2 alternative fragment length(s) may be 1,36,125,568 bps INFO @ Wed, 08 Dec 2021 19:06:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10_model.r WARNING @ Wed, 08 Dec 2021 19:06:53: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 19:06:53: #2 You may need to consider one of the other alternative d(s): 1,36,125,568 WARNING @ Wed, 08 Dec 2021 19:06:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 19:06:53: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:06:53: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:06:56: 72000000 INFO @ Wed, 08 Dec 2021 19:06:59: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:07:00: 73000000 INFO @ Wed, 08 Dec 2021 19:07:04: 74000000 INFO @ Wed, 08 Dec 2021 19:07:08: 75000000 INFO @ Wed, 08 Dec 2021 19:07:11: 76000000 INFO @ Wed, 08 Dec 2021 19:07:15: 77000000 INFO @ Wed, 08 Dec 2021 19:07:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05_peaks.xls INFO @ Wed, 08 Dec 2021 19:07:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:07:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.05_summits.bed INFO @ Wed, 08 Dec 2021 19:07:16: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:07:18: #1 tag size is determined as 38 bps INFO @ Wed, 08 Dec 2021 19:07:18: #1 tag size = 38 INFO @ Wed, 08 Dec 2021 19:07:18: #1 total tags in treatment: 33225175 INFO @ Wed, 08 Dec 2021 19:07:18: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:07:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:07:19: #1 tags after filtering in treatment: 28664814 INFO @ Wed, 08 Dec 2021 19:07:19: #1 Redundant rate of treatment: 0.14 INFO @ Wed, 08 Dec 2021 19:07:19: #1 finished! INFO @ Wed, 08 Dec 2021 19:07:19: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:07:20: #2 number of paired peaks: 145 WARNING @ Wed, 08 Dec 2021 19:07:20: Fewer paired peaks (145) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 145 pairs to build model! INFO @ Wed, 08 Dec 2021 19:07:20: start model_add_line... INFO @ Wed, 08 Dec 2021 19:07:21: start X-correlation... INFO @ Wed, 08 Dec 2021 19:07:21: end of X-cor INFO @ Wed, 08 Dec 2021 19:07:21: #2 finished! INFO @ Wed, 08 Dec 2021 19:07:21: #2 predicted fragment length is 1 bps INFO @ Wed, 08 Dec 2021 19:07:21: #2 alternative fragment length(s) may be 1,36,125,568 bps INFO @ Wed, 08 Dec 2021 19:07:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20_model.r WARNING @ Wed, 08 Dec 2021 19:07:21: #2 Since the d (1) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Wed, 08 Dec 2021 19:07:21: #2 You may need to consider one of the other alternative d(s): 1,36,125,568 WARNING @ Wed, 08 Dec 2021 19:07:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Wed, 08 Dec 2021 19:07:21: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:07:21: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:07:30: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 19:07:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10_peaks.xls INFO @ Wed, 08 Dec 2021 19:07:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:07:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.10_summits.bed INFO @ Wed, 08 Dec 2021 19:07:47: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:07:58: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:08:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20_peaks.xls INFO @ Wed, 08 Dec 2021 19:08:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:08:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX10342585/SRX10342585.20_summits.bed INFO @ Wed, 08 Dec 2021 19:08:14: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling