Job ID = 6366274 SRX = SRX1030170 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:45:22 prefetch.2.10.7: 1) Downloading 'SRR2029636'... 2020-06-15T22:45:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:46:31 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:46:32 prefetch.2.10.7: 'SRR2029636' is valid 2020-06-15T22:46:32 prefetch.2.10.7: 1) 'SRR2029636' was downloaded successfully 2020-06-15T22:47:05 prefetch.2.10.7: 'SRR2029636' has 6 unresolved dependencies 2020-06-15T22:47:05 prefetch.2.10.7: 2) Downloading 'ncbi-acc:BX284601.4?vdb-ctx=refseq'... 2020-06-15T22:47:05 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:47:22 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:47:22 prefetch.2.10.7: 2) 'ncbi-acc:BX284601.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T22:47:22 prefetch.2.10.7: 3) Downloading 'ncbi-acc:BX284602.4?vdb-ctx=refseq'... 2020-06-15T22:47:22 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:47:37 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:47:37 prefetch.2.10.7: 3) 'ncbi-acc:BX284602.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T22:47:37 prefetch.2.10.7: 4) Downloading 'ncbi-acc:BX284603.3?vdb-ctx=refseq'... 2020-06-15T22:47:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:47:53 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:47:53 prefetch.2.10.7: 4) 'ncbi-acc:BX284603.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T22:47:53 prefetch.2.10.7: 5) Downloading 'ncbi-acc:BX284604.3?vdb-ctx=refseq'... 2020-06-15T22:47:53 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:48:09 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:48:09 prefetch.2.10.7: 5) 'ncbi-acc:BX284604.3?vdb-ctx=refseq' was downloaded successfully 2020-06-15T22:48:09 prefetch.2.10.7: 6) Downloading 'ncbi-acc:BX284605.4?vdb-ctx=refseq'... 2020-06-15T22:48:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:48:26 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:48:26 prefetch.2.10.7: 6) 'ncbi-acc:BX284605.4?vdb-ctx=refseq' was downloaded successfully 2020-06-15T22:48:26 prefetch.2.10.7: 7) Downloading 'ncbi-acc:BX284606.4?vdb-ctx=refseq'... 2020-06-15T22:48:26 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:48:42 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:48:42 prefetch.2.10.7: 7) 'ncbi-acc:BX284606.4?vdb-ctx=refseq' was downloaded successfully Read 24819951 spots for SRR2029636/SRR2029636.sra Written 24819951 spots for SRR2029636/SRR2029636.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:47 24819951 reads; of these: 24819951 (100.00%) were unpaired; of these: 7126 (0.03%) aligned 0 times 23079960 (92.99%) aligned exactly 1 time 1732865 (6.98%) aligned >1 times 99.97% overall alignment rate Time searching: 00:03:47 Overall time: 00:03:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 1013333 / 24812825 = 0.0408 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:59:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:59:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:59:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:59:56: 1000000 INFO @ Tue, 16 Jun 2020 08:00:02: 2000000 INFO @ Tue, 16 Jun 2020 08:00:09: 3000000 INFO @ Tue, 16 Jun 2020 08:00:15: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:00:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:00:19: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:00:19: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:00:22: 5000000 INFO @ Tue, 16 Jun 2020 08:00:26: 1000000 INFO @ Tue, 16 Jun 2020 08:00:29: 6000000 INFO @ Tue, 16 Jun 2020 08:00:33: 2000000 INFO @ Tue, 16 Jun 2020 08:00:36: 7000000 INFO @ Tue, 16 Jun 2020 08:00:40: 3000000 INFO @ Tue, 16 Jun 2020 08:00:43: 8000000 INFO @ Tue, 16 Jun 2020 08:00:46: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:00:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:00:49: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:00:49: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:00:50: 9000000 INFO @ Tue, 16 Jun 2020 08:00:53: 5000000 INFO @ Tue, 16 Jun 2020 08:00:56: 1000000 INFO @ Tue, 16 Jun 2020 08:00:57: 10000000 INFO @ Tue, 16 Jun 2020 08:01:00: 6000000 INFO @ Tue, 16 Jun 2020 08:01:02: 2000000 INFO @ Tue, 16 Jun 2020 08:01:04: 11000000 INFO @ Tue, 16 Jun 2020 08:01:07: 7000000 INFO @ Tue, 16 Jun 2020 08:01:09: 3000000 INFO @ Tue, 16 Jun 2020 08:01:11: 12000000 INFO @ Tue, 16 Jun 2020 08:01:14: 8000000 INFO @ Tue, 16 Jun 2020 08:01:16: 4000000 INFO @ Tue, 16 Jun 2020 08:01:18: 13000000 INFO @ Tue, 16 Jun 2020 08:01:21: 9000000 INFO @ Tue, 16 Jun 2020 08:01:23: 5000000 INFO @ Tue, 16 Jun 2020 08:01:25: 14000000 INFO @ Tue, 16 Jun 2020 08:01:28: 10000000 INFO @ Tue, 16 Jun 2020 08:01:30: 6000000 INFO @ Tue, 16 Jun 2020 08:01:32: 15000000 INFO @ Tue, 16 Jun 2020 08:01:34: 11000000 INFO @ Tue, 16 Jun 2020 08:01:36: 7000000 INFO @ Tue, 16 Jun 2020 08:01:39: 16000000 INFO @ Tue, 16 Jun 2020 08:01:41: 12000000 INFO @ Tue, 16 Jun 2020 08:01:44: 8000000 INFO @ Tue, 16 Jun 2020 08:01:46: 17000000 INFO @ Tue, 16 Jun 2020 08:01:49: 13000000 INFO @ Tue, 16 Jun 2020 08:01:51: 9000000 INFO @ Tue, 16 Jun 2020 08:01:53: 18000000 INFO @ Tue, 16 Jun 2020 08:01:56: 14000000 INFO @ Tue, 16 Jun 2020 08:01:57: 10000000 INFO @ Tue, 16 Jun 2020 08:02:00: 19000000 INFO @ Tue, 16 Jun 2020 08:02:03: 15000000 INFO @ Tue, 16 Jun 2020 08:02:04: 11000000 INFO @ Tue, 16 Jun 2020 08:02:07: 20000000 INFO @ Tue, 16 Jun 2020 08:02:10: 16000000 INFO @ Tue, 16 Jun 2020 08:02:11: 12000000 INFO @ Tue, 16 Jun 2020 08:02:14: 21000000 INFO @ Tue, 16 Jun 2020 08:02:17: 17000000 INFO @ Tue, 16 Jun 2020 08:02:18: 13000000 INFO @ Tue, 16 Jun 2020 08:02:21: 22000000 INFO @ Tue, 16 Jun 2020 08:02:24: 18000000 INFO @ Tue, 16 Jun 2020 08:02:25: 14000000 INFO @ Tue, 16 Jun 2020 08:02:29: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:02:31: 19000000 INFO @ Tue, 16 Jun 2020 08:02:33: 15000000 INFO @ Tue, 16 Jun 2020 08:02:35: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:02:35: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:02:35: #1 total tags in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:02:35: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:02:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:02:35: #1 tags after filtering in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:02:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:02:35: #1 finished! INFO @ Tue, 16 Jun 2020 08:02:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:02:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:02:37: #2 number of paired peaks: 6 WARNING @ Tue, 16 Jun 2020 08:02:37: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:02:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:02:39: 20000000 INFO @ Tue, 16 Jun 2020 08:02:39: 16000000 INFO @ Tue, 16 Jun 2020 08:02:46: 21000000 INFO @ Tue, 16 Jun 2020 08:02:47: 17000000 INFO @ Tue, 16 Jun 2020 08:02:53: 22000000 INFO @ Tue, 16 Jun 2020 08:02:54: 18000000 INFO @ Tue, 16 Jun 2020 08:03:00: 23000000 INFO @ Tue, 16 Jun 2020 08:03:01: 19000000 INFO @ Tue, 16 Jun 2020 08:03:06: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:03:06: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:03:06: #1 total tags in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:03:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:03:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:03:06: #1 tags after filtering in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:03:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:03:06: #1 finished! INFO @ Tue, 16 Jun 2020 08:03:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:03:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:03:07: 20000000 INFO @ Tue, 16 Jun 2020 08:03:08: #2 number of paired peaks: 6 WARNING @ Tue, 16 Jun 2020 08:03:08: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:03:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:03:14: 21000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:03:20: 22000000 INFO @ Tue, 16 Jun 2020 08:03:27: 23000000 INFO @ Tue, 16 Jun 2020 08:03:32: #1 tag size is determined as 51 bps INFO @ Tue, 16 Jun 2020 08:03:32: #1 tag size = 51 INFO @ Tue, 16 Jun 2020 08:03:32: #1 total tags in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:03:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:03:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:03:33: #1 tags after filtering in treatment: 23799492 INFO @ Tue, 16 Jun 2020 08:03:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:03:33: #1 finished! INFO @ Tue, 16 Jun 2020 08:03:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:03:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:03:34: #2 number of paired peaks: 6 WARNING @ Tue, 16 Jun 2020 08:03:34: Too few paired peaks (6) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:03:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX1030170/SRX1030170.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling