Job ID = 14160141 SRX = SRX10144728 Genome = ce11 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:29 53318775 reads; of these: 53318775 (100.00%) were paired; of these: 18630615 (34.94%) aligned concordantly 0 times 29825416 (55.94%) aligned concordantly exactly 1 time 4862744 (9.12%) aligned concordantly >1 times ---- 18630615 pairs aligned concordantly 0 times; of these: 1265822 (6.79%) aligned discordantly 1 time ---- 17364793 pairs aligned 0 times concordantly or discordantly; of these: 34729586 mates make up the pairs; of these: 33826647 (97.40%) aligned 0 times 476185 (1.37%) aligned exactly 1 time 426754 (1.23%) aligned >1 times 68.28% overall alignment rate Time searching: 00:28:29 Overall time: 00:28:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 8811952 / 35800889 = 0.2461 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:38:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:38:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:38:20: 1000000 INFO @ Thu, 09 Dec 2021 01:38:24: 2000000 INFO @ Thu, 09 Dec 2021 01:38:28: 3000000 INFO @ Thu, 09 Dec 2021 01:38:32: 4000000 INFO @ Thu, 09 Dec 2021 01:38:36: 5000000 INFO @ Thu, 09 Dec 2021 01:38:40: 6000000 INFO @ Thu, 09 Dec 2021 01:38:43: 7000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:38:46: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:38:46: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:38:47: 8000000 INFO @ Thu, 09 Dec 2021 01:38:50: 1000000 INFO @ Thu, 09 Dec 2021 01:38:51: 9000000 INFO @ Thu, 09 Dec 2021 01:38:54: 2000000 INFO @ Thu, 09 Dec 2021 01:38:55: 10000000 INFO @ Thu, 09 Dec 2021 01:38:58: 3000000 INFO @ Thu, 09 Dec 2021 01:38:59: 11000000 INFO @ Thu, 09 Dec 2021 01:39:02: 4000000 INFO @ Thu, 09 Dec 2021 01:39:03: 12000000 INFO @ Thu, 09 Dec 2021 01:39:06: 5000000 INFO @ Thu, 09 Dec 2021 01:39:07: 13000000 INFO @ Thu, 09 Dec 2021 01:39:10: 6000000 INFO @ Thu, 09 Dec 2021 01:39:11: 14000000 INFO @ Thu, 09 Dec 2021 01:39:14: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 01:39:15: 15000000 INFO @ Thu, 09 Dec 2021 01:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 01:39:16: #1 read tag files... INFO @ Thu, 09 Dec 2021 01:39:16: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 01:39:18: 8000000 INFO @ Thu, 09 Dec 2021 01:39:19: 16000000 INFO @ Thu, 09 Dec 2021 01:39:21: 1000000 INFO @ Thu, 09 Dec 2021 01:39:22: 9000000 INFO @ Thu, 09 Dec 2021 01:39:23: 17000000 INFO @ Thu, 09 Dec 2021 01:39:25: 2000000 INFO @ Thu, 09 Dec 2021 01:39:26: 10000000 INFO @ Thu, 09 Dec 2021 01:39:27: 18000000 INFO @ Thu, 09 Dec 2021 01:39:30: 3000000 INFO @ Thu, 09 Dec 2021 01:39:30: 11000000 INFO @ Thu, 09 Dec 2021 01:39:31: 19000000 INFO @ Thu, 09 Dec 2021 01:39:34: 12000000 INFO @ Thu, 09 Dec 2021 01:39:35: 4000000 INFO @ Thu, 09 Dec 2021 01:39:35: 20000000 INFO @ Thu, 09 Dec 2021 01:39:38: 13000000 INFO @ Thu, 09 Dec 2021 01:39:39: 21000000 INFO @ Thu, 09 Dec 2021 01:39:40: 5000000 INFO @ Thu, 09 Dec 2021 01:39:42: 14000000 INFO @ Thu, 09 Dec 2021 01:39:43: 22000000 INFO @ Thu, 09 Dec 2021 01:39:44: 6000000 INFO @ Thu, 09 Dec 2021 01:39:46: 15000000 INFO @ Thu, 09 Dec 2021 01:39:47: 23000000 INFO @ Thu, 09 Dec 2021 01:39:49: 7000000 INFO @ Thu, 09 Dec 2021 01:39:51: 16000000 INFO @ Thu, 09 Dec 2021 01:39:51: 24000000 INFO @ Thu, 09 Dec 2021 01:39:53: 8000000 INFO @ Thu, 09 Dec 2021 01:39:55: 17000000 INFO @ Thu, 09 Dec 2021 01:39:55: 25000000 INFO @ Thu, 09 Dec 2021 01:39:58: 9000000 INFO @ Thu, 09 Dec 2021 01:39:59: 18000000 INFO @ Thu, 09 Dec 2021 01:39:59: 26000000 INFO @ Thu, 09 Dec 2021 01:40:03: 19000000 INFO @ Thu, 09 Dec 2021 01:40:03: 10000000 INFO @ Thu, 09 Dec 2021 01:40:03: 27000000 INFO @ Thu, 09 Dec 2021 01:40:07: 20000000 INFO @ Thu, 09 Dec 2021 01:40:07: 28000000 INFO @ Thu, 09 Dec 2021 01:40:07: 11000000 INFO @ Thu, 09 Dec 2021 01:40:11: 21000000 INFO @ Thu, 09 Dec 2021 01:40:11: 29000000 INFO @ Thu, 09 Dec 2021 01:40:12: 12000000 INFO @ Thu, 09 Dec 2021 01:40:15: 22000000 INFO @ Thu, 09 Dec 2021 01:40:15: 30000000 INFO @ Thu, 09 Dec 2021 01:40:17: 13000000 INFO @ Thu, 09 Dec 2021 01:40:19: 23000000 INFO @ Thu, 09 Dec 2021 01:40:19: 31000000 INFO @ Thu, 09 Dec 2021 01:40:21: 14000000 INFO @ Thu, 09 Dec 2021 01:40:23: 24000000 INFO @ Thu, 09 Dec 2021 01:40:23: 32000000 INFO @ Thu, 09 Dec 2021 01:40:26: 15000000 INFO @ Thu, 09 Dec 2021 01:40:27: 25000000 INFO @ Thu, 09 Dec 2021 01:40:27: 33000000 INFO @ Thu, 09 Dec 2021 01:40:31: 16000000 INFO @ Thu, 09 Dec 2021 01:40:31: 26000000 INFO @ Thu, 09 Dec 2021 01:40:31: 34000000 INFO @ Thu, 09 Dec 2021 01:40:35: 27000000 INFO @ Thu, 09 Dec 2021 01:40:35: 35000000 INFO @ Thu, 09 Dec 2021 01:40:35: 17000000 INFO @ Thu, 09 Dec 2021 01:40:39: 28000000 INFO @ Thu, 09 Dec 2021 01:40:39: 36000000 INFO @ Thu, 09 Dec 2021 01:40:40: 18000000 INFO @ Thu, 09 Dec 2021 01:40:43: 29000000 INFO @ Thu, 09 Dec 2021 01:40:43: 37000000 INFO @ Thu, 09 Dec 2021 01:40:45: 19000000 INFO @ Thu, 09 Dec 2021 01:40:47: 30000000 INFO @ Thu, 09 Dec 2021 01:40:47: 38000000 INFO @ Thu, 09 Dec 2021 01:40:49: 20000000 INFO @ Thu, 09 Dec 2021 01:40:51: 39000000 INFO @ Thu, 09 Dec 2021 01:40:51: 31000000 INFO @ Thu, 09 Dec 2021 01:40:54: 21000000 INFO @ Thu, 09 Dec 2021 01:40:55: 40000000 INFO @ Thu, 09 Dec 2021 01:40:55: 32000000 INFO @ Thu, 09 Dec 2021 01:40:58: 22000000 INFO @ Thu, 09 Dec 2021 01:40:59: 41000000 INFO @ Thu, 09 Dec 2021 01:40:59: 33000000 INFO @ Thu, 09 Dec 2021 01:41:02: 42000000 INFO @ Thu, 09 Dec 2021 01:41:03: 34000000 INFO @ Thu, 09 Dec 2021 01:41:03: 23000000 INFO @ Thu, 09 Dec 2021 01:41:06: 43000000 INFO @ Thu, 09 Dec 2021 01:41:07: 35000000 INFO @ Thu, 09 Dec 2021 01:41:08: 24000000 INFO @ Thu, 09 Dec 2021 01:41:10: 44000000 INFO @ Thu, 09 Dec 2021 01:41:11: 36000000 INFO @ Thu, 09 Dec 2021 01:41:12: 25000000 INFO @ Thu, 09 Dec 2021 01:41:14: 45000000 INFO @ Thu, 09 Dec 2021 01:41:15: 37000000 INFO @ Thu, 09 Dec 2021 01:41:17: 26000000 INFO @ Thu, 09 Dec 2021 01:41:18: 46000000 INFO @ Thu, 09 Dec 2021 01:41:19: 38000000 INFO @ Thu, 09 Dec 2021 01:41:21: 27000000 INFO @ Thu, 09 Dec 2021 01:41:22: 47000000 INFO @ Thu, 09 Dec 2021 01:41:23: 39000000 INFO @ Thu, 09 Dec 2021 01:41:26: 28000000 INFO @ Thu, 09 Dec 2021 01:41:26: 48000000 INFO @ Thu, 09 Dec 2021 01:41:27: 40000000 INFO @ Thu, 09 Dec 2021 01:41:30: 49000000 INFO @ Thu, 09 Dec 2021 01:41:30: 29000000 INFO @ Thu, 09 Dec 2021 01:41:31: 41000000 INFO @ Thu, 09 Dec 2021 01:41:34: 50000000 INFO @ Thu, 09 Dec 2021 01:41:35: 42000000 INFO @ Thu, 09 Dec 2021 01:41:35: 30000000 INFO @ Thu, 09 Dec 2021 01:41:38: 51000000 INFO @ Thu, 09 Dec 2021 01:41:39: 43000000 INFO @ Thu, 09 Dec 2021 01:41:40: 31000000 INFO @ Thu, 09 Dec 2021 01:41:42: 52000000 INFO @ Thu, 09 Dec 2021 01:41:43: 44000000 INFO @ Thu, 09 Dec 2021 01:41:44: 32000000 INFO @ Thu, 09 Dec 2021 01:41:46: 53000000 INFO @ Thu, 09 Dec 2021 01:41:47: 45000000 INFO @ Thu, 09 Dec 2021 01:41:49: 33000000 INFO @ Thu, 09 Dec 2021 01:41:50: 54000000 INFO @ Thu, 09 Dec 2021 01:41:51: 46000000 INFO @ Thu, 09 Dec 2021 01:41:53: 34000000 INFO @ Thu, 09 Dec 2021 01:41:54: 55000000 INFO @ Thu, 09 Dec 2021 01:41:55: #1 tag size is determined as 42 bps INFO @ Thu, 09 Dec 2021 01:41:55: #1 tag size = 42 INFO @ Thu, 09 Dec 2021 01:41:55: #1 total tags in treatment: 26013432 INFO @ Thu, 09 Dec 2021 01:41:55: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:41:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:41:55: 47000000 INFO @ Thu, 09 Dec 2021 01:41:55: #1 tags after filtering in treatment: 17963812 INFO @ Thu, 09 Dec 2021 01:41:55: #1 Redundant rate of treatment: 0.31 INFO @ Thu, 09 Dec 2021 01:41:55: #1 finished! INFO @ Thu, 09 Dec 2021 01:41:55: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:41:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:41:56: #2 number of paired peaks: 69 WARNING @ Thu, 09 Dec 2021 01:41:56: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 01:41:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:41:58: 35000000 INFO @ Thu, 09 Dec 2021 01:41:59: 48000000 INFO @ Thu, 09 Dec 2021 01:42:02: 36000000 INFO @ Thu, 09 Dec 2021 01:42:03: 49000000 INFO @ Thu, 09 Dec 2021 01:42:07: 50000000 INFO @ Thu, 09 Dec 2021 01:42:07: 37000000 INFO @ Thu, 09 Dec 2021 01:42:11: 51000000 INFO @ Thu, 09 Dec 2021 01:42:11: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 09 Dec 2021 01:42:15: 52000000 INFO @ Thu, 09 Dec 2021 01:42:16: 39000000 INFO @ Thu, 09 Dec 2021 01:42:19: 53000000 INFO @ Thu, 09 Dec 2021 01:42:20: 40000000 INFO @ Thu, 09 Dec 2021 01:42:22: 54000000 INFO @ Thu, 09 Dec 2021 01:42:25: 41000000 INFO @ Thu, 09 Dec 2021 01:42:26: 55000000 INFO @ Thu, 09 Dec 2021 01:42:27: #1 tag size is determined as 42 bps INFO @ Thu, 09 Dec 2021 01:42:27: #1 tag size = 42 INFO @ Thu, 09 Dec 2021 01:42:27: #1 total tags in treatment: 26013432 INFO @ Thu, 09 Dec 2021 01:42:27: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:42:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:42:28: #1 tags after filtering in treatment: 17963812 INFO @ Thu, 09 Dec 2021 01:42:28: #1 Redundant rate of treatment: 0.31 INFO @ Thu, 09 Dec 2021 01:42:28: #1 finished! INFO @ Thu, 09 Dec 2021 01:42:28: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:42:28: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:42:29: #2 number of paired peaks: 69 WARNING @ Thu, 09 Dec 2021 01:42:29: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 01:42:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 09 Dec 2021 01:42:29: 42000000 INFO @ Thu, 09 Dec 2021 01:42:34: 43000000 INFO @ Thu, 09 Dec 2021 01:42:38: 44000000 INFO @ Thu, 09 Dec 2021 01:42:43: 45000000 INFO @ Thu, 09 Dec 2021 01:42:47: 46000000 INFO @ Thu, 09 Dec 2021 01:42:52: 47000000 INFO @ Thu, 09 Dec 2021 01:42:56: 48000000 INFO @ Thu, 09 Dec 2021 01:43:01: 49000000 INFO @ Thu, 09 Dec 2021 01:43:05: 50000000 INFO @ Thu, 09 Dec 2021 01:43:09: 51000000 INFO @ Thu, 09 Dec 2021 01:43:14: 52000000 BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 01:43:18: 53000000 INFO @ Thu, 09 Dec 2021 01:43:23: 54000000 INFO @ Thu, 09 Dec 2021 01:43:27: 55000000 INFO @ Thu, 09 Dec 2021 01:43:28: #1 tag size is determined as 42 bps INFO @ Thu, 09 Dec 2021 01:43:28: #1 tag size = 42 INFO @ Thu, 09 Dec 2021 01:43:28: #1 total tags in treatment: 26013432 INFO @ Thu, 09 Dec 2021 01:43:28: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 01:43:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 01:43:29: #1 tags after filtering in treatment: 17963812 INFO @ Thu, 09 Dec 2021 01:43:29: #1 Redundant rate of treatment: 0.31 INFO @ Thu, 09 Dec 2021 01:43:29: #1 finished! INFO @ Thu, 09 Dec 2021 01:43:29: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 01:43:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 01:43:30: #2 number of paired peaks: 69 WARNING @ Thu, 09 Dec 2021 01:43:30: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 09 Dec 2021 01:43:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10144728/SRX10144728.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling