Job ID = 14158102 SRX = SRX10040021 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:56 56175430 reads; of these: 56175430 (100.00%) were unpaired; of these: 8941916 (15.92%) aligned 0 times 38634134 (68.77%) aligned exactly 1 time 8599380 (15.31%) aligned >1 times 84.08% overall alignment rate Time searching: 00:10:56 Overall time: 00:10:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8693960 / 47233514 = 0.1841 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:43:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:43:20: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:43:20: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:43:26: 1000000 INFO @ Wed, 08 Dec 2021 14:43:32: 2000000 INFO @ Wed, 08 Dec 2021 14:43:38: 3000000 INFO @ Wed, 08 Dec 2021 14:43:44: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:43:50: 5000000 INFO @ Wed, 08 Dec 2021 14:43:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:43:50: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:43:50: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:43:56: 6000000 INFO @ Wed, 08 Dec 2021 14:43:56: 1000000 INFO @ Wed, 08 Dec 2021 14:44:01: 2000000 INFO @ Wed, 08 Dec 2021 14:44:02: 7000000 INFO @ Wed, 08 Dec 2021 14:44:07: 3000000 INFO @ Wed, 08 Dec 2021 14:44:08: 8000000 INFO @ Wed, 08 Dec 2021 14:44:12: 4000000 INFO @ Wed, 08 Dec 2021 14:44:14: 9000000 INFO @ Wed, 08 Dec 2021 14:44:17: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:44:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:44:20: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:44:20: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:44:20: 10000000 INFO @ Wed, 08 Dec 2021 14:44:22: 6000000 INFO @ Wed, 08 Dec 2021 14:44:27: 11000000 INFO @ Wed, 08 Dec 2021 14:44:27: 1000000 INFO @ Wed, 08 Dec 2021 14:44:28: 7000000 INFO @ Wed, 08 Dec 2021 14:44:33: 2000000 INFO @ Wed, 08 Dec 2021 14:44:33: 12000000 INFO @ Wed, 08 Dec 2021 14:44:33: 8000000 INFO @ Wed, 08 Dec 2021 14:44:39: 9000000 INFO @ Wed, 08 Dec 2021 14:44:39: 3000000 INFO @ Wed, 08 Dec 2021 14:44:39: 13000000 INFO @ Wed, 08 Dec 2021 14:44:44: 10000000 INFO @ Wed, 08 Dec 2021 14:44:45: 4000000 INFO @ Wed, 08 Dec 2021 14:44:45: 14000000 INFO @ Wed, 08 Dec 2021 14:44:50: 11000000 INFO @ Wed, 08 Dec 2021 14:44:51: 5000000 INFO @ Wed, 08 Dec 2021 14:44:51: 15000000 INFO @ Wed, 08 Dec 2021 14:44:55: 12000000 INFO @ Wed, 08 Dec 2021 14:44:57: 6000000 INFO @ Wed, 08 Dec 2021 14:44:58: 16000000 INFO @ Wed, 08 Dec 2021 14:45:01: 13000000 INFO @ Wed, 08 Dec 2021 14:45:03: 7000000 INFO @ Wed, 08 Dec 2021 14:45:04: 17000000 INFO @ Wed, 08 Dec 2021 14:45:06: 14000000 INFO @ Wed, 08 Dec 2021 14:45:09: 8000000 INFO @ Wed, 08 Dec 2021 14:45:10: 18000000 INFO @ Wed, 08 Dec 2021 14:45:11: 15000000 INFO @ Wed, 08 Dec 2021 14:45:15: 9000000 INFO @ Wed, 08 Dec 2021 14:45:16: 19000000 INFO @ Wed, 08 Dec 2021 14:45:17: 16000000 INFO @ Wed, 08 Dec 2021 14:45:22: 10000000 INFO @ Wed, 08 Dec 2021 14:45:22: 17000000 INFO @ Wed, 08 Dec 2021 14:45:23: 20000000 INFO @ Wed, 08 Dec 2021 14:45:28: 11000000 INFO @ Wed, 08 Dec 2021 14:45:28: 18000000 INFO @ Wed, 08 Dec 2021 14:45:29: 21000000 INFO @ Wed, 08 Dec 2021 14:45:33: 19000000 INFO @ Wed, 08 Dec 2021 14:45:34: 12000000 INFO @ Wed, 08 Dec 2021 14:45:35: 22000000 INFO @ Wed, 08 Dec 2021 14:45:39: 20000000 INFO @ Wed, 08 Dec 2021 14:45:40: 13000000 INFO @ Wed, 08 Dec 2021 14:45:41: 23000000 INFO @ Wed, 08 Dec 2021 14:45:44: 21000000 INFO @ Wed, 08 Dec 2021 14:45:46: 14000000 INFO @ Wed, 08 Dec 2021 14:45:47: 24000000 INFO @ Wed, 08 Dec 2021 14:45:50: 22000000 INFO @ Wed, 08 Dec 2021 14:45:53: 15000000 INFO @ Wed, 08 Dec 2021 14:45:54: 25000000 INFO @ Wed, 08 Dec 2021 14:45:55: 23000000 INFO @ Wed, 08 Dec 2021 14:45:59: 16000000 INFO @ Wed, 08 Dec 2021 14:46:00: 26000000 INFO @ Wed, 08 Dec 2021 14:46:01: 24000000 INFO @ Wed, 08 Dec 2021 14:46:05: 17000000 INFO @ Wed, 08 Dec 2021 14:46:06: 25000000 INFO @ Wed, 08 Dec 2021 14:46:06: 27000000 INFO @ Wed, 08 Dec 2021 14:46:11: 18000000 INFO @ Wed, 08 Dec 2021 14:46:12: 26000000 INFO @ Wed, 08 Dec 2021 14:46:12: 28000000 INFO @ Wed, 08 Dec 2021 14:46:17: 27000000 INFO @ Wed, 08 Dec 2021 14:46:17: 19000000 INFO @ Wed, 08 Dec 2021 14:46:19: 29000000 INFO @ Wed, 08 Dec 2021 14:46:23: 28000000 INFO @ Wed, 08 Dec 2021 14:46:24: 20000000 INFO @ Wed, 08 Dec 2021 14:46:25: 30000000 INFO @ Wed, 08 Dec 2021 14:46:28: 29000000 INFO @ Wed, 08 Dec 2021 14:46:30: 21000000 INFO @ Wed, 08 Dec 2021 14:46:31: 31000000 INFO @ Wed, 08 Dec 2021 14:46:33: 30000000 INFO @ Wed, 08 Dec 2021 14:46:36: 22000000 INFO @ Wed, 08 Dec 2021 14:46:37: 32000000 INFO @ Wed, 08 Dec 2021 14:46:39: 31000000 INFO @ Wed, 08 Dec 2021 14:46:42: 23000000 INFO @ Wed, 08 Dec 2021 14:46:43: 33000000 INFO @ Wed, 08 Dec 2021 14:46:44: 32000000 INFO @ Wed, 08 Dec 2021 14:46:48: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:46:50: 34000000 INFO @ Wed, 08 Dec 2021 14:46:50: 33000000 INFO @ Wed, 08 Dec 2021 14:46:55: 25000000 INFO @ Wed, 08 Dec 2021 14:46:55: 34000000 INFO @ Wed, 08 Dec 2021 14:46:56: 35000000 INFO @ Wed, 08 Dec 2021 14:47:01: 35000000 INFO @ Wed, 08 Dec 2021 14:47:01: 26000000 INFO @ Wed, 08 Dec 2021 14:47:02: 36000000 INFO @ Wed, 08 Dec 2021 14:47:06: 36000000 INFO @ Wed, 08 Dec 2021 14:47:07: 27000000 INFO @ Wed, 08 Dec 2021 14:47:08: 37000000 INFO @ Wed, 08 Dec 2021 14:47:12: 37000000 INFO @ Wed, 08 Dec 2021 14:47:13: 28000000 INFO @ Wed, 08 Dec 2021 14:47:14: 38000000 INFO @ Wed, 08 Dec 2021 14:47:17: 38000000 INFO @ Wed, 08 Dec 2021 14:47:18: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:47:18: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:47:18: #1 total tags in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:47:18: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:47:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:47:19: #1 tags after filtering in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:47:19: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:47:19: #1 finished! INFO @ Wed, 08 Dec 2021 14:47:19: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:47:19: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:47:20: 29000000 INFO @ Wed, 08 Dec 2021 14:47:20: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:47:20: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:47:20: #1 total tags in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:47:20: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:47:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:47:21: #1 tags after filtering in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:47:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:47:21: #1 finished! INFO @ Wed, 08 Dec 2021 14:47:21: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:47:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:47:21: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:47:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:47:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:47:23: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:47:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:47:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:47:26: 30000000 INFO @ Wed, 08 Dec 2021 14:47:32: 31000000 INFO @ Wed, 08 Dec 2021 14:47:38: 32000000 INFO @ Wed, 08 Dec 2021 14:47:44: 33000000 INFO @ Wed, 08 Dec 2021 14:47:50: 34000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:47:56: 35000000 INFO @ Wed, 08 Dec 2021 14:48:02: 36000000 INFO @ Wed, 08 Dec 2021 14:48:08: 37000000 INFO @ Wed, 08 Dec 2021 14:48:14: 38000000 INFO @ Wed, 08 Dec 2021 14:48:17: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:48:17: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:48:17: #1 total tags in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:48:17: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:48:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:48:17: #1 tags after filtering in treatment: 38539554 INFO @ Wed, 08 Dec 2021 14:48:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:48:17: #1 finished! INFO @ Wed, 08 Dec 2021 14:48:17: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:48:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:48:20: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 14:48:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:48:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040021/SRX10040021.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling