Job ID = 14158090 SRX = SRX10040018 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:54 50621464 reads; of these: 50621464 (100.00%) were unpaired; of these: 3266124 (6.45%) aligned 0 times 36198238 (71.51%) aligned exactly 1 time 11157102 (22.04%) aligned >1 times 93.55% overall alignment rate Time searching: 00:09:54 Overall time: 00:09:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 12264360 / 47355340 = 0.2590 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:37:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:37:46: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:37:46: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:37:51: 1000000 INFO @ Wed, 08 Dec 2021 14:37:56: 2000000 INFO @ Wed, 08 Dec 2021 14:38:01: 3000000 INFO @ Wed, 08 Dec 2021 14:38:05: 4000000 INFO @ Wed, 08 Dec 2021 14:38:10: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:38:15: 6000000 INFO @ Wed, 08 Dec 2021 14:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:38:16: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:38:16: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:38:20: 7000000 INFO @ Wed, 08 Dec 2021 14:38:23: 1000000 INFO @ Wed, 08 Dec 2021 14:38:26: 8000000 INFO @ Wed, 08 Dec 2021 14:38:29: 2000000 INFO @ Wed, 08 Dec 2021 14:38:31: 9000000 INFO @ Wed, 08 Dec 2021 14:38:35: 3000000 INFO @ Wed, 08 Dec 2021 14:38:36: 10000000 INFO @ Wed, 08 Dec 2021 14:38:41: 4000000 INFO @ Wed, 08 Dec 2021 14:38:42: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:38:46: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:38:46: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:38:47: 12000000 INFO @ Wed, 08 Dec 2021 14:38:48: 5000000 INFO @ Wed, 08 Dec 2021 14:38:52: 1000000 INFO @ Wed, 08 Dec 2021 14:38:53: 13000000 INFO @ Wed, 08 Dec 2021 14:38:54: 6000000 INFO @ Wed, 08 Dec 2021 14:38:57: 2000000 INFO @ Wed, 08 Dec 2021 14:38:58: 14000000 INFO @ Wed, 08 Dec 2021 14:39:00: 7000000 INFO @ Wed, 08 Dec 2021 14:39:03: 3000000 INFO @ Wed, 08 Dec 2021 14:39:03: 15000000 INFO @ Wed, 08 Dec 2021 14:39:05: 8000000 INFO @ Wed, 08 Dec 2021 14:39:08: 4000000 INFO @ Wed, 08 Dec 2021 14:39:09: 16000000 INFO @ Wed, 08 Dec 2021 14:39:11: 9000000 INFO @ Wed, 08 Dec 2021 14:39:14: 5000000 INFO @ Wed, 08 Dec 2021 14:39:14: 17000000 INFO @ Wed, 08 Dec 2021 14:39:18: 10000000 INFO @ Wed, 08 Dec 2021 14:39:19: 6000000 INFO @ Wed, 08 Dec 2021 14:39:19: 18000000 INFO @ Wed, 08 Dec 2021 14:39:24: 11000000 INFO @ Wed, 08 Dec 2021 14:39:24: 7000000 INFO @ Wed, 08 Dec 2021 14:39:25: 19000000 INFO @ Wed, 08 Dec 2021 14:39:30: 12000000 INFO @ Wed, 08 Dec 2021 14:39:30: 8000000 INFO @ Wed, 08 Dec 2021 14:39:30: 20000000 INFO @ Wed, 08 Dec 2021 14:39:35: 9000000 INFO @ Wed, 08 Dec 2021 14:39:35: 21000000 INFO @ Wed, 08 Dec 2021 14:39:36: 13000000 INFO @ Wed, 08 Dec 2021 14:39:41: 10000000 INFO @ Wed, 08 Dec 2021 14:39:41: 22000000 INFO @ Wed, 08 Dec 2021 14:39:42: 14000000 INFO @ Wed, 08 Dec 2021 14:39:46: 11000000 INFO @ Wed, 08 Dec 2021 14:39:46: 23000000 INFO @ Wed, 08 Dec 2021 14:39:48: 15000000 INFO @ Wed, 08 Dec 2021 14:39:52: 12000000 INFO @ Wed, 08 Dec 2021 14:39:52: 24000000 INFO @ Wed, 08 Dec 2021 14:39:54: 16000000 INFO @ Wed, 08 Dec 2021 14:39:57: 13000000 INFO @ Wed, 08 Dec 2021 14:39:57: 25000000 INFO @ Wed, 08 Dec 2021 14:40:00: 17000000 INFO @ Wed, 08 Dec 2021 14:40:02: 14000000 INFO @ Wed, 08 Dec 2021 14:40:02: 26000000 INFO @ Wed, 08 Dec 2021 14:40:06: 18000000 INFO @ Wed, 08 Dec 2021 14:40:08: 15000000 INFO @ Wed, 08 Dec 2021 14:40:08: 27000000 INFO @ Wed, 08 Dec 2021 14:40:12: 19000000 INFO @ Wed, 08 Dec 2021 14:40:13: 16000000 INFO @ Wed, 08 Dec 2021 14:40:13: 28000000 INFO @ Wed, 08 Dec 2021 14:40:18: 20000000 INFO @ Wed, 08 Dec 2021 14:40:19: 17000000 INFO @ Wed, 08 Dec 2021 14:40:19: 29000000 INFO @ Wed, 08 Dec 2021 14:40:24: 18000000 INFO @ Wed, 08 Dec 2021 14:40:24: 21000000 INFO @ Wed, 08 Dec 2021 14:40:24: 30000000 INFO @ Wed, 08 Dec 2021 14:40:29: 19000000 INFO @ Wed, 08 Dec 2021 14:40:30: 31000000 INFO @ Wed, 08 Dec 2021 14:40:30: 22000000 INFO @ Wed, 08 Dec 2021 14:40:35: 20000000 INFO @ Wed, 08 Dec 2021 14:40:35: 32000000 INFO @ Wed, 08 Dec 2021 14:40:36: 23000000 INFO @ Wed, 08 Dec 2021 14:40:40: 21000000 INFO @ Wed, 08 Dec 2021 14:40:41: 33000000 INFO @ Wed, 08 Dec 2021 14:40:42: 24000000 INFO @ Wed, 08 Dec 2021 14:40:45: 22000000 INFO @ Wed, 08 Dec 2021 14:40:46: 34000000 INFO @ Wed, 08 Dec 2021 14:40:48: 25000000 INFO @ Wed, 08 Dec 2021 14:40:51: 23000000 INFO @ Wed, 08 Dec 2021 14:40:51: 35000000 INFO @ Wed, 08 Dec 2021 14:40:52: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:40:52: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:40:52: #1 total tags in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:40:52: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:40:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:40:53: #1 tags after filtering in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:40:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:40:53: #1 finished! INFO @ Wed, 08 Dec 2021 14:40:53: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:40:53: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:40:54: 26000000 INFO @ Wed, 08 Dec 2021 14:40:55: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 14:40:55: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:40:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:40:56: 24000000 INFO @ Wed, 08 Dec 2021 14:41:00: 27000000 INFO @ Wed, 08 Dec 2021 14:41:01: 25000000 INFO @ Wed, 08 Dec 2021 14:41:06: 28000000 INFO @ Wed, 08 Dec 2021 14:41:07: 26000000 INFO @ Wed, 08 Dec 2021 14:41:12: 27000000 INFO @ Wed, 08 Dec 2021 14:41:12: 29000000 INFO @ Wed, 08 Dec 2021 14:41:18: 28000000 INFO @ Wed, 08 Dec 2021 14:41:18: 30000000 INFO @ Wed, 08 Dec 2021 14:41:23: 29000000 INFO @ Wed, 08 Dec 2021 14:41:25: 31000000 INFO @ Wed, 08 Dec 2021 14:41:28: 30000000 INFO @ Wed, 08 Dec 2021 14:41:31: 32000000 INFO @ Wed, 08 Dec 2021 14:41:34: 31000000 INFO @ Wed, 08 Dec 2021 14:41:37: 33000000 INFO @ Wed, 08 Dec 2021 14:41:39: 32000000 INFO @ Wed, 08 Dec 2021 14:41:43: 34000000 INFO @ Wed, 08 Dec 2021 14:41:45: 33000000 INFO @ Wed, 08 Dec 2021 14:41:49: 35000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:41:50: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:41:50: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:41:50: #1 total tags in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:41:50: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:41:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:41:50: 34000000 INFO @ Wed, 08 Dec 2021 14:41:50: #1 tags after filtering in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:41:50: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:41:50: #1 finished! INFO @ Wed, 08 Dec 2021 14:41:50: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:41:50: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:41:53: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 14:41:53: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:41:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:41:55: 35000000 INFO @ Wed, 08 Dec 2021 14:41:56: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:41:56: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:41:56: #1 total tags in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:41:56: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:41:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:41:57: #1 tags after filtering in treatment: 35090980 INFO @ Wed, 08 Dec 2021 14:41:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:41:57: #1 finished! INFO @ Wed, 08 Dec 2021 14:41:57: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:41:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:41:59: #2 number of paired peaks: 24 WARNING @ Wed, 08 Dec 2021 14:41:59: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:41:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040018/SRX10040018.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling