Job ID = 14158061 SRX = SRX10040007 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:02 42456586 reads; of these: 42456586 (100.00%) were unpaired; of these: 3462917 (8.16%) aligned 0 times 31462032 (74.10%) aligned exactly 1 time 7531637 (17.74%) aligned >1 times 91.84% overall alignment rate Time searching: 00:09:02 Overall time: 00:09:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7014411 / 38993669 = 0.1799 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:25:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:25:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:25:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:25:08: 1000000 INFO @ Wed, 08 Dec 2021 14:25:14: 2000000 INFO @ Wed, 08 Dec 2021 14:25:20: 3000000 INFO @ Wed, 08 Dec 2021 14:25:26: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:25:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:25:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:25:32: 5000000 INFO @ Wed, 08 Dec 2021 14:25:38: 1000000 INFO @ Wed, 08 Dec 2021 14:25:39: 6000000 INFO @ Wed, 08 Dec 2021 14:25:45: 2000000 INFO @ Wed, 08 Dec 2021 14:25:45: 7000000 INFO @ Wed, 08 Dec 2021 14:25:51: 3000000 INFO @ Wed, 08 Dec 2021 14:25:52: 8000000 INFO @ Wed, 08 Dec 2021 14:25:58: 4000000 INFO @ Wed, 08 Dec 2021 14:25:58: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 14:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 14:26:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 14:26:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 14:26:04: 5000000 INFO @ Wed, 08 Dec 2021 14:26:04: 10000000 INFO @ Wed, 08 Dec 2021 14:26:08: 1000000 INFO @ Wed, 08 Dec 2021 14:26:10: 6000000 INFO @ Wed, 08 Dec 2021 14:26:11: 11000000 INFO @ Wed, 08 Dec 2021 14:26:15: 2000000 INFO @ Wed, 08 Dec 2021 14:26:17: 12000000 INFO @ Wed, 08 Dec 2021 14:26:17: 7000000 INFO @ Wed, 08 Dec 2021 14:26:21: 3000000 INFO @ Wed, 08 Dec 2021 14:26:23: 13000000 INFO @ Wed, 08 Dec 2021 14:26:23: 8000000 INFO @ Wed, 08 Dec 2021 14:26:27: 4000000 INFO @ Wed, 08 Dec 2021 14:26:30: 14000000 INFO @ Wed, 08 Dec 2021 14:26:30: 9000000 INFO @ Wed, 08 Dec 2021 14:26:34: 5000000 INFO @ Wed, 08 Dec 2021 14:26:36: 15000000 INFO @ Wed, 08 Dec 2021 14:26:36: 10000000 INFO @ Wed, 08 Dec 2021 14:26:40: 6000000 INFO @ Wed, 08 Dec 2021 14:26:42: 16000000 INFO @ Wed, 08 Dec 2021 14:26:42: 11000000 INFO @ Wed, 08 Dec 2021 14:26:46: 7000000 INFO @ Wed, 08 Dec 2021 14:26:49: 17000000 INFO @ Wed, 08 Dec 2021 14:26:49: 12000000 INFO @ Wed, 08 Dec 2021 14:26:53: 8000000 INFO @ Wed, 08 Dec 2021 14:26:55: 18000000 INFO @ Wed, 08 Dec 2021 14:26:55: 13000000 INFO @ Wed, 08 Dec 2021 14:26:59: 9000000 INFO @ Wed, 08 Dec 2021 14:27:01: 19000000 INFO @ Wed, 08 Dec 2021 14:27:01: 14000000 INFO @ Wed, 08 Dec 2021 14:27:05: 10000000 INFO @ Wed, 08 Dec 2021 14:27:07: 20000000 INFO @ Wed, 08 Dec 2021 14:27:08: 15000000 INFO @ Wed, 08 Dec 2021 14:27:12: 11000000 INFO @ Wed, 08 Dec 2021 14:27:13: 21000000 INFO @ Wed, 08 Dec 2021 14:27:14: 16000000 INFO @ Wed, 08 Dec 2021 14:27:18: 12000000 INFO @ Wed, 08 Dec 2021 14:27:20: 22000000 INFO @ Wed, 08 Dec 2021 14:27:20: 17000000 INFO @ Wed, 08 Dec 2021 14:27:24: 13000000 INFO @ Wed, 08 Dec 2021 14:27:26: 23000000 INFO @ Wed, 08 Dec 2021 14:27:27: 18000000 INFO @ Wed, 08 Dec 2021 14:27:30: 14000000 INFO @ Wed, 08 Dec 2021 14:27:32: 24000000 INFO @ Wed, 08 Dec 2021 14:27:33: 19000000 INFO @ Wed, 08 Dec 2021 14:27:36: 15000000 INFO @ Wed, 08 Dec 2021 14:27:38: 25000000 INFO @ Wed, 08 Dec 2021 14:27:39: 20000000 INFO @ Wed, 08 Dec 2021 14:27:43: 16000000 INFO @ Wed, 08 Dec 2021 14:27:45: 26000000 INFO @ Wed, 08 Dec 2021 14:27:46: 21000000 INFO @ Wed, 08 Dec 2021 14:27:49: 17000000 INFO @ Wed, 08 Dec 2021 14:27:51: 27000000 INFO @ Wed, 08 Dec 2021 14:27:52: 22000000 INFO @ Wed, 08 Dec 2021 14:27:55: 18000000 INFO @ Wed, 08 Dec 2021 14:27:57: 28000000 INFO @ Wed, 08 Dec 2021 14:27:58: 23000000 INFO @ Wed, 08 Dec 2021 14:28:01: 19000000 INFO @ Wed, 08 Dec 2021 14:28:04: 29000000 INFO @ Wed, 08 Dec 2021 14:28:04: 24000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 14:28:08: 20000000 INFO @ Wed, 08 Dec 2021 14:28:10: 30000000 INFO @ Wed, 08 Dec 2021 14:28:10: 25000000 INFO @ Wed, 08 Dec 2021 14:28:14: 21000000 INFO @ Wed, 08 Dec 2021 14:28:17: 31000000 INFO @ Wed, 08 Dec 2021 14:28:17: 26000000 INFO @ Wed, 08 Dec 2021 14:28:20: 22000000 INFO @ Wed, 08 Dec 2021 14:28:23: 27000000 INFO @ Wed, 08 Dec 2021 14:28:23: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:28:23: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:28:23: #1 total tags in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:28:23: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:28:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:28:24: #1 tags after filtering in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:28:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:28:24: #1 finished! INFO @ Wed, 08 Dec 2021 14:28:24: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:28:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:28:26: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 14:28:26: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:28:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:28:26: 23000000 INFO @ Wed, 08 Dec 2021 14:28:29: 28000000 INFO @ Wed, 08 Dec 2021 14:28:32: 24000000 INFO @ Wed, 08 Dec 2021 14:28:35: 29000000 INFO @ Wed, 08 Dec 2021 14:28:38: 25000000 INFO @ Wed, 08 Dec 2021 14:28:42: 30000000 INFO @ Wed, 08 Dec 2021 14:28:44: 26000000 INFO @ Wed, 08 Dec 2021 14:28:48: 31000000 INFO @ Wed, 08 Dec 2021 14:28:51: 27000000 INFO @ Wed, 08 Dec 2021 14:28:54: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:28:54: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:28:54: #1 total tags in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:28:54: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:28:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:28:55: #1 tags after filtering in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:28:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:28:55: #1 finished! INFO @ Wed, 08 Dec 2021 14:28:55: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:28:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:28:57: 28000000 INFO @ Wed, 08 Dec 2021 14:28:57: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 14:28:57: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:28:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 14:29:02: 29000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 14:29:08: 30000000 INFO @ Wed, 08 Dec 2021 14:29:14: 31000000 INFO @ Wed, 08 Dec 2021 14:29:20: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 14:29:20: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 14:29:20: #1 total tags in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:29:20: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 14:29:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 14:29:21: #1 tags after filtering in treatment: 31979258 INFO @ Wed, 08 Dec 2021 14:29:21: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 14:29:21: #1 finished! INFO @ Wed, 08 Dec 2021 14:29:21: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 14:29:21: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 14:29:22: #2 number of paired peaks: 57 WARNING @ Wed, 08 Dec 2021 14:29:22: Too few paired peaks (57) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 14:29:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX10040007/SRX10040007.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling