Job ID = 6366236 SRX = SRX080090 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:49:37 prefetch.2.10.7: 1) Downloading 'SRR298910'... 2020-06-15T22:49:37 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:49:59 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:50:00 prefetch.2.10.7: 'SRR298910' is valid 2020-06-15T22:50:00 prefetch.2.10.7: 1) 'SRR298910' was downloaded successfully Read 5139276 spots for SRR298910/SRR298910.sra Written 5139276 spots for SRR298910/SRR298910.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:00:28 5139276 reads; of these: 5139276 (100.00%) were unpaired; of these: 3309536 (64.40%) aligned 0 times 1546528 (30.09%) aligned exactly 1 time 283212 (5.51%) aligned >1 times 35.60% overall alignment rate Time searching: 00:00:29 Overall time: 00:00:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 302690 / 1829740 = 0.1654 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:51:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:51:40: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:51:40: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:51:45: 1000000 INFO @ Tue, 16 Jun 2020 07:51:48: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:51:48: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:51:48: #1 total tags in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:51:48: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:51:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:51:48: #1 tags after filtering in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:51:48: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:51:48: #1 finished! INFO @ Tue, 16 Jun 2020 07:51:48: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:51:48: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:51:48: #2 number of paired peaks: 1579 INFO @ Tue, 16 Jun 2020 07:51:48: start model_add_line... INFO @ Tue, 16 Jun 2020 07:51:48: start X-correlation... INFO @ Tue, 16 Jun 2020 07:51:48: end of X-cor INFO @ Tue, 16 Jun 2020 07:51:48: #2 finished! INFO @ Tue, 16 Jun 2020 07:51:48: #2 predicted fragment length is 129 bps INFO @ Tue, 16 Jun 2020 07:51:48: #2 alternative fragment length(s) may be 129 bps INFO @ Tue, 16 Jun 2020 07:51:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05_model.r INFO @ Tue, 16 Jun 2020 07:51:48: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:51:48: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:51:52: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:51:54: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:51:54: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:51:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.05_summits.bed INFO @ Tue, 16 Jun 2020 07:51:54: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2108 records, 4 fields): 5 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:10: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:10: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:16: 1000000 INFO @ Tue, 16 Jun 2020 07:52:19: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:52:19: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:52:19: #1 total tags in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:52:19: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:52:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:52:19: #1 tags after filtering in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:52:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:52:19: #1 finished! INFO @ Tue, 16 Jun 2020 07:52:19: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:52:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:52:19: #2 number of paired peaks: 1579 INFO @ Tue, 16 Jun 2020 07:52:19: start model_add_line... INFO @ Tue, 16 Jun 2020 07:52:19: start X-correlation... INFO @ Tue, 16 Jun 2020 07:52:19: end of X-cor INFO @ Tue, 16 Jun 2020 07:52:19: #2 finished! INFO @ Tue, 16 Jun 2020 07:52:19: #2 predicted fragment length is 129 bps INFO @ Tue, 16 Jun 2020 07:52:19: #2 alternative fragment length(s) may be 129 bps INFO @ Tue, 16 Jun 2020 07:52:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10_model.r INFO @ Tue, 16 Jun 2020 07:52:19: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:52:19: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:52:23: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:52:25: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:52:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:52:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.10_summits.bed INFO @ Tue, 16 Jun 2020 07:52:25: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1435 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:52:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:52:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:52:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:52:48: 1000000 INFO @ Tue, 16 Jun 2020 07:52:52: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:52:52: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:52:52: #1 total tags in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:52:52: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:52:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:52:52: #1 tags after filtering in treatment: 1527050 INFO @ Tue, 16 Jun 2020 07:52:52: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:52:52: #1 finished! INFO @ Tue, 16 Jun 2020 07:52:52: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:52:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:52:52: #2 number of paired peaks: 1579 INFO @ Tue, 16 Jun 2020 07:52:52: start model_add_line... INFO @ Tue, 16 Jun 2020 07:52:52: start X-correlation... INFO @ Tue, 16 Jun 2020 07:52:52: end of X-cor INFO @ Tue, 16 Jun 2020 07:52:52: #2 finished! INFO @ Tue, 16 Jun 2020 07:52:52: #2 predicted fragment length is 129 bps INFO @ Tue, 16 Jun 2020 07:52:52: #2 alternative fragment length(s) may be 129 bps INFO @ Tue, 16 Jun 2020 07:52:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20_model.r INFO @ Tue, 16 Jun 2020 07:52:52: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:52:52: #3 Pre-compute pvalue-qvalue table... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:52:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:52:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:52:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:52:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX080090/SRX080090.20_summits.bed INFO @ Tue, 16 Jun 2020 07:52:58: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (884 records, 4 fields): 3 millis CompletedMACS2peakCalling BigWig に変換しました。