Job ID = 6366185 SRX = SRX065710 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:32:36 prefetch.2.10.7: 1) Downloading 'SRR217416'... 2020-06-15T22:32:36 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:33:20 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:33:20 prefetch.2.10.7: 'SRR217416' is valid 2020-06-15T22:33:20 prefetch.2.10.7: 1) 'SRR217416' was downloaded successfully Read 6286196 spots for SRR217416/SRR217416.sra Written 6286196 spots for SRR217416/SRR217416.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:49 6286196 reads; of these: 6286196 (100.00%) were unpaired; of these: 2208712 (35.14%) aligned 0 times 3359199 (53.44%) aligned exactly 1 time 718285 (11.43%) aligned >1 times 64.86% overall alignment rate Time searching: 00:00:49 Overall time: 00:00:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 361032 / 4077484 = 0.0885 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:35:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:35:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:35:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:35:54: 1000000 INFO @ Tue, 16 Jun 2020 07:36:00: 2000000 INFO @ Tue, 16 Jun 2020 07:36:06: 3000000 INFO @ Tue, 16 Jun 2020 07:36:10: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:36:10: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:36:10: #1 total tags in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:36:10: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:36:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:36:10: #1 tags after filtering in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:36:10: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:36:10: #1 finished! INFO @ Tue, 16 Jun 2020 07:36:10: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:36:10: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:36:11: #2 number of paired peaks: 652 WARNING @ Tue, 16 Jun 2020 07:36:11: Fewer paired peaks (652) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 652 pairs to build model! INFO @ Tue, 16 Jun 2020 07:36:11: start model_add_line... INFO @ Tue, 16 Jun 2020 07:36:11: start X-correlation... INFO @ Tue, 16 Jun 2020 07:36:11: end of X-cor INFO @ Tue, 16 Jun 2020 07:36:11: #2 finished! INFO @ Tue, 16 Jun 2020 07:36:11: #2 predicted fragment length is 39 bps INFO @ Tue, 16 Jun 2020 07:36:11: #2 alternative fragment length(s) may be 4,39,71 bps INFO @ Tue, 16 Jun 2020 07:36:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05_model.r WARNING @ Tue, 16 Jun 2020 07:36:11: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:36:11: #2 You may need to consider one of the other alternative d(s): 4,39,71 WARNING @ Tue, 16 Jun 2020 07:36:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:36:11: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:36:11: #3 Pre-compute pvalue-qvalue table... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:36:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:36:18: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:36:18: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:36:19: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:36:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:36:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:36:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.05_summits.bed INFO @ Tue, 16 Jun 2020 07:36:23: Done! INFO @ Tue, 16 Jun 2020 07:36:23: 1000000 pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (836 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:36:29: 2000000 INFO @ Tue, 16 Jun 2020 07:36:34: 3000000 INFO @ Tue, 16 Jun 2020 07:36:37: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:36:37: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:36:37: #1 total tags in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:36:37: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:36:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:36:37: #1 tags after filtering in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:36:37: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:36:37: #1 finished! INFO @ Tue, 16 Jun 2020 07:36:37: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:36:37: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:36:38: #2 number of paired peaks: 652 WARNING @ Tue, 16 Jun 2020 07:36:38: Fewer paired peaks (652) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 652 pairs to build model! INFO @ Tue, 16 Jun 2020 07:36:38: start model_add_line... INFO @ Tue, 16 Jun 2020 07:36:38: start X-correlation... INFO @ Tue, 16 Jun 2020 07:36:38: end of X-cor INFO @ Tue, 16 Jun 2020 07:36:38: #2 finished! INFO @ Tue, 16 Jun 2020 07:36:38: #2 predicted fragment length is 39 bps INFO @ Tue, 16 Jun 2020 07:36:38: #2 alternative fragment length(s) may be 4,39,71 bps INFO @ Tue, 16 Jun 2020 07:36:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10_model.r WARNING @ Tue, 16 Jun 2020 07:36:38: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:36:38: #2 You may need to consider one of the other alternative d(s): 4,39,71 WARNING @ Tue, 16 Jun 2020 07:36:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:36:38: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:36:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:36:46: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:36:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:36:48: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:36:48: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:36:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:36:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:36:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.10_summits.bed INFO @ Tue, 16 Jun 2020 07:36:50: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (347 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:36:53: 1000000 INFO @ Tue, 16 Jun 2020 07:36:58: 2000000 INFO @ Tue, 16 Jun 2020 07:37:04: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:37:07: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:37:07: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:37:07: #1 total tags in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:37:07: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:37:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:37:07: #1 tags after filtering in treatment: 3716452 INFO @ Tue, 16 Jun 2020 07:37:07: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:37:07: #1 finished! INFO @ Tue, 16 Jun 2020 07:37:07: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:37:07: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:37:08: #2 number of paired peaks: 652 WARNING @ Tue, 16 Jun 2020 07:37:08: Fewer paired peaks (652) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 652 pairs to build model! INFO @ Tue, 16 Jun 2020 07:37:08: start model_add_line... INFO @ Tue, 16 Jun 2020 07:37:08: start X-correlation... INFO @ Tue, 16 Jun 2020 07:37:08: end of X-cor INFO @ Tue, 16 Jun 2020 07:37:08: #2 finished! INFO @ Tue, 16 Jun 2020 07:37:08: #2 predicted fragment length is 39 bps INFO @ Tue, 16 Jun 2020 07:37:08: #2 alternative fragment length(s) may be 4,39,71 bps INFO @ Tue, 16 Jun 2020 07:37:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20_model.r WARNING @ Tue, 16 Jun 2020 07:37:08: #2 Since the d (39) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:37:08: #2 You may need to consider one of the other alternative d(s): 4,39,71 WARNING @ Tue, 16 Jun 2020 07:37:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:37:08: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:37:08: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:37:16: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:37:20: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:37:20: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:37:20: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065710/SRX065710.20_summits.bed INFO @ Tue, 16 Jun 2020 07:37:20: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (71 records, 4 fields): 1 millis CompletedMACS2peakCalling