Job ID = 6366139 SRX = SRX065655 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:00:38 prefetch.2.10.7: 1) Downloading 'SRR217361'... 2020-06-15T23:00:38 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:01:07 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:01:07 prefetch.2.10.7: 'SRR217361' is valid 2020-06-15T23:01:07 prefetch.2.10.7: 1) 'SRR217361' was downloaded successfully Read 5382681 spots for SRR217361/SRR217361.sra Written 5382681 spots for SRR217361/SRR217361.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:44 5382681 reads; of these: 5382681 (100.00%) were unpaired; of these: 1876576 (34.86%) aligned 0 times 2958927 (54.97%) aligned exactly 1 time 547178 (10.17%) aligned >1 times 65.14% overall alignment rate Time searching: 00:00:44 Overall time: 00:00:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 177977 / 3506105 = 0.0508 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:03:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:03:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:03:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:03:22: 1000000 INFO @ Tue, 16 Jun 2020 08:03:28: 2000000 INFO @ Tue, 16 Jun 2020 08:03:33: 3000000 INFO @ Tue, 16 Jun 2020 08:03:35: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:03:35: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:03:35: #1 total tags in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:03:35: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:03:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:03:35: #1 tags after filtering in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:03:35: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:03:35: #1 finished! INFO @ Tue, 16 Jun 2020 08:03:35: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:03:35: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:03:35: #2 number of paired peaks: 420 WARNING @ Tue, 16 Jun 2020 08:03:35: Fewer paired peaks (420) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 420 pairs to build model! INFO @ Tue, 16 Jun 2020 08:03:35: start model_add_line... INFO @ Tue, 16 Jun 2020 08:03:35: start X-correlation... INFO @ Tue, 16 Jun 2020 08:03:35: end of X-cor INFO @ Tue, 16 Jun 2020 08:03:35: #2 finished! INFO @ Tue, 16 Jun 2020 08:03:35: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 08:03:35: #2 alternative fragment length(s) may be 4,31,420,448,593 bps INFO @ Tue, 16 Jun 2020 08:03:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05_model.r WARNING @ Tue, 16 Jun 2020 08:03:35: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:03:35: #2 You may need to consider one of the other alternative d(s): 4,31,420,448,593 WARNING @ Tue, 16 Jun 2020 08:03:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:03:35: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:03:35: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:03:43: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:03:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05_peaks.xls INFO @ Tue, 16 Jun 2020 08:03:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:03:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.05_summits.bed INFO @ Tue, 16 Jun 2020 08:03:47: Done! INFO @ Tue, 16 Jun 2020 08:03:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:03:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:03:47: #1 read treatment tags... pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (312 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:03:53: 1000000 INFO @ Tue, 16 Jun 2020 08:03:58: 2000000 INFO @ Tue, 16 Jun 2020 08:04:04: 3000000 INFO @ Tue, 16 Jun 2020 08:04:06: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:04:06: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:04:06: #1 total tags in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:04:06: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:04:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:04:06: #1 tags after filtering in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:04:06: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:04:06: #1 finished! INFO @ Tue, 16 Jun 2020 08:04:06: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:04:06: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:04:06: #2 number of paired peaks: 420 WARNING @ Tue, 16 Jun 2020 08:04:06: Fewer paired peaks (420) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 420 pairs to build model! INFO @ Tue, 16 Jun 2020 08:04:06: start model_add_line... INFO @ Tue, 16 Jun 2020 08:04:06: start X-correlation... INFO @ Tue, 16 Jun 2020 08:04:06: end of X-cor INFO @ Tue, 16 Jun 2020 08:04:06: #2 finished! INFO @ Tue, 16 Jun 2020 08:04:06: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 08:04:06: #2 alternative fragment length(s) may be 4,31,420,448,593 bps INFO @ Tue, 16 Jun 2020 08:04:06: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10_model.r WARNING @ Tue, 16 Jun 2020 08:04:06: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:04:06: #2 You may need to consider one of the other alternative d(s): 4,31,420,448,593 WARNING @ Tue, 16 Jun 2020 08:04:06: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:04:06: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:04:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 08:04:14: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:04:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:04:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:04:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 08:04:18: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10_peaks.xls INFO @ Tue, 16 Jun 2020 08:04:18: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:04:18: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.10_summits.bed INFO @ Tue, 16 Jun 2020 08:04:18: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (126 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 08:04:23: 1000000 INFO @ Tue, 16 Jun 2020 08:04:30: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 08:04:36: 3000000 INFO @ Tue, 16 Jun 2020 08:04:38: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 08:04:38: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 08:04:38: #1 total tags in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:04:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:04:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:04:38: #1 tags after filtering in treatment: 3328128 INFO @ Tue, 16 Jun 2020 08:04:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 08:04:38: #1 finished! INFO @ Tue, 16 Jun 2020 08:04:38: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:04:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:04:38: #2 number of paired peaks: 420 WARNING @ Tue, 16 Jun 2020 08:04:38: Fewer paired peaks (420) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 420 pairs to build model! INFO @ Tue, 16 Jun 2020 08:04:38: start model_add_line... INFO @ Tue, 16 Jun 2020 08:04:38: start X-correlation... INFO @ Tue, 16 Jun 2020 08:04:38: end of X-cor INFO @ Tue, 16 Jun 2020 08:04:38: #2 finished! INFO @ Tue, 16 Jun 2020 08:04:38: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 08:04:38: #2 alternative fragment length(s) may be 4,31,420,448,593 bps INFO @ Tue, 16 Jun 2020 08:04:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20_model.r WARNING @ Tue, 16 Jun 2020 08:04:38: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 08:04:38: #2 You may need to consider one of the other alternative d(s): 4,31,420,448,593 WARNING @ Tue, 16 Jun 2020 08:04:38: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 08:04:38: #3 Call peaks... INFO @ Tue, 16 Jun 2020 08:04:38: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:04:46: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 08:04:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20_peaks.xls INFO @ Tue, 16 Jun 2020 08:04:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 08:04:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065655/SRX065655.20_summits.bed INFO @ Tue, 16 Jun 2020 08:04:50: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (22 records, 4 fields): 1 millis CompletedMACS2peakCalling