Job ID = 6366120 SRX = SRX065636 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:33:06 prefetch.2.10.7: 1) Downloading 'SRR217342'... 2020-06-15T22:33:06 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:33:37 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:33:37 prefetch.2.10.7: 'SRR217342' is valid 2020-06-15T22:33:37 prefetch.2.10.7: 1) 'SRR217342' was downloaded successfully Read 5310923 spots for SRR217342/SRR217342.sra Written 5310923 spots for SRR217342/SRR217342.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:23 5310923 reads; of these: 5310923 (100.00%) were unpaired; of these: 4173320 (78.58%) aligned 0 times 969604 (18.26%) aligned exactly 1 time 167999 (3.16%) aligned >1 times 21.42% overall alignment rate Time searching: 00:00:23 Overall time: 00:00:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 62841 / 1137603 = 0.0552 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:35:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:35:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:35:03: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:35:08: 1000000 INFO @ Tue, 16 Jun 2020 07:35:08: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:35:08: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:35:08: #1 total tags in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:35:08: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:35:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:35:08: #1 tags after filtering in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:35:08: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:35:08: #1 finished! INFO @ Tue, 16 Jun 2020 07:35:08: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:35:08: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:35:08: #2 number of paired peaks: 890 WARNING @ Tue, 16 Jun 2020 07:35:08: Fewer paired peaks (890) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 890 pairs to build model! INFO @ Tue, 16 Jun 2020 07:35:08: start model_add_line... INFO @ Tue, 16 Jun 2020 07:35:08: start X-correlation... INFO @ Tue, 16 Jun 2020 07:35:08: end of X-cor INFO @ Tue, 16 Jun 2020 07:35:08: #2 finished! INFO @ Tue, 16 Jun 2020 07:35:08: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:35:08: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:35:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05_model.r INFO @ Tue, 16 Jun 2020 07:35:08: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:35:08: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:35:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:35:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:35:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:35:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.05_summits.bed INFO @ Tue, 16 Jun 2020 07:35:12: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (314 records, 4 fields): 2 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:35:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:35:33: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:35:33: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:35:38: 1000000 INFO @ Tue, 16 Jun 2020 07:35:38: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:35:38: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:35:38: #1 total tags in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:35:38: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:35:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:35:38: #1 tags after filtering in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:35:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:35:38: #1 finished! INFO @ Tue, 16 Jun 2020 07:35:38: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:35:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:35:38: #2 number of paired peaks: 890 WARNING @ Tue, 16 Jun 2020 07:35:38: Fewer paired peaks (890) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 890 pairs to build model! INFO @ Tue, 16 Jun 2020 07:35:38: start model_add_line... INFO @ Tue, 16 Jun 2020 07:35:38: start X-correlation... INFO @ Tue, 16 Jun 2020 07:35:38: end of X-cor INFO @ Tue, 16 Jun 2020 07:35:38: #2 finished! INFO @ Tue, 16 Jun 2020 07:35:38: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:35:38: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:35:38: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10_model.r INFO @ Tue, 16 Jun 2020 07:35:38: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:35:38: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:35:41: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:35:42: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:35:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:35:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.10_summits.bed INFO @ Tue, 16 Jun 2020 07:35:42: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (96 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:36:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:36:03: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:36:03: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:36:09: 1000000 INFO @ Tue, 16 Jun 2020 07:36:09: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:36:09: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:36:09: #1 total tags in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:36:09: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:36:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:36:09: #1 tags after filtering in treatment: 1074762 INFO @ Tue, 16 Jun 2020 07:36:09: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:36:09: #1 finished! INFO @ Tue, 16 Jun 2020 07:36:09: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:36:09: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:36:09: #2 number of paired peaks: 890 WARNING @ Tue, 16 Jun 2020 07:36:09: Fewer paired peaks (890) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 890 pairs to build model! INFO @ Tue, 16 Jun 2020 07:36:09: start model_add_line... INFO @ Tue, 16 Jun 2020 07:36:09: start X-correlation... INFO @ Tue, 16 Jun 2020 07:36:09: end of X-cor INFO @ Tue, 16 Jun 2020 07:36:09: #2 finished! INFO @ Tue, 16 Jun 2020 07:36:09: #2 predicted fragment length is 115 bps INFO @ Tue, 16 Jun 2020 07:36:09: #2 alternative fragment length(s) may be 115 bps INFO @ Tue, 16 Jun 2020 07:36:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20_model.r INFO @ Tue, 16 Jun 2020 07:36:09: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:36:09: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:36:12: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:36:13: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:36:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:36:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065636/SRX065636.20_summits.bed INFO @ Tue, 16 Jun 2020 07:36:13: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (21 records, 4 fields): 1 millis CompletedMACS2peakCalling