Job ID = 6366117 SRX = SRX065633 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:51:41 prefetch.2.10.7: 1) Downloading 'SRR217339'... 2020-06-15T22:51:41 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:52:08 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:52:08 prefetch.2.10.7: 'SRR217339' is valid 2020-06-15T22:52:08 prefetch.2.10.7: 1) 'SRR217339' was downloaded successfully Read 5717141 spots for SRR217339/SRR217339.sra Written 5717141 spots for SRR217339/SRR217339.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:50 5717141 reads; of these: 5717141 (100.00%) were unpaired; of these: 1268518 (22.19%) aligned 0 times 3805721 (66.57%) aligned exactly 1 time 642902 (11.25%) aligned >1 times 77.81% overall alignment rate Time searching: 00:00:50 Overall time: 00:00:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 1255016 / 4448623 = 0.2821 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:54:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:54:45: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:54:45: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:54:51: 1000000 INFO @ Tue, 16 Jun 2020 07:54:57: 2000000 INFO @ Tue, 16 Jun 2020 07:55:02: 3000000 INFO @ Tue, 16 Jun 2020 07:55:03: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:55:03: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:55:03: #1 total tags in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:55:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:03: #1 tags after filtering in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:55:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:03: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:04: #2 number of paired peaks: 636 WARNING @ Tue, 16 Jun 2020 07:55:04: Fewer paired peaks (636) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 636 pairs to build model! INFO @ Tue, 16 Jun 2020 07:55:04: start model_add_line... INFO @ Tue, 16 Jun 2020 07:55:04: start X-correlation... INFO @ Tue, 16 Jun 2020 07:55:04: end of X-cor INFO @ Tue, 16 Jun 2020 07:55:04: #2 finished! INFO @ Tue, 16 Jun 2020 07:55:04: #2 predicted fragment length is 128 bps INFO @ Tue, 16 Jun 2020 07:55:04: #2 alternative fragment length(s) may be 128,597 bps INFO @ Tue, 16 Jun 2020 07:55:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05_model.r INFO @ Tue, 16 Jun 2020 07:55:04: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:55:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:55:11: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:55:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:55:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.05_summits.bed INFO @ Tue, 16 Jun 2020 07:55:14: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (2378 records, 4 fields): 109 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:15: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:15: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:21: 1000000 INFO @ Tue, 16 Jun 2020 07:55:26: 2000000 INFO @ Tue, 16 Jun 2020 07:55:32: 3000000 INFO @ Tue, 16 Jun 2020 07:55:33: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:55:33: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:55:33: #1 total tags in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:55:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:55:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:55:33: #1 tags after filtering in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:55:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:55:33: #1 finished! INFO @ Tue, 16 Jun 2020 07:55:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:55:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:55:33: #2 number of paired peaks: 636 WARNING @ Tue, 16 Jun 2020 07:55:33: Fewer paired peaks (636) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 636 pairs to build model! INFO @ Tue, 16 Jun 2020 07:55:33: start model_add_line... INFO @ Tue, 16 Jun 2020 07:55:33: start X-correlation... INFO @ Tue, 16 Jun 2020 07:55:33: end of X-cor INFO @ Tue, 16 Jun 2020 07:55:33: #2 finished! INFO @ Tue, 16 Jun 2020 07:55:33: #2 predicted fragment length is 128 bps INFO @ Tue, 16 Jun 2020 07:55:33: #2 alternative fragment length(s) may be 128,597 bps INFO @ Tue, 16 Jun 2020 07:55:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10_model.r INFO @ Tue, 16 Jun 2020 07:55:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:55:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:55:40: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:55:44: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:55:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:55:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.10_summits.bed INFO @ Tue, 16 Jun 2020 07:55:44: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (947 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:55:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:55:45: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:55:45: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:55:51: 1000000 INFO @ Tue, 16 Jun 2020 07:55:57: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:56:03: 3000000 INFO @ Tue, 16 Jun 2020 07:56:04: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:56:04: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:56:04: #1 total tags in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:56:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:56:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:56:04: #1 tags after filtering in treatment: 3193607 INFO @ Tue, 16 Jun 2020 07:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:56:04: #1 finished! INFO @ Tue, 16 Jun 2020 07:56:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:56:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:56:04: #2 number of paired peaks: 636 WARNING @ Tue, 16 Jun 2020 07:56:04: Fewer paired peaks (636) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 636 pairs to build model! INFO @ Tue, 16 Jun 2020 07:56:04: start model_add_line... INFO @ Tue, 16 Jun 2020 07:56:04: start X-correlation... INFO @ Tue, 16 Jun 2020 07:56:04: end of X-cor INFO @ Tue, 16 Jun 2020 07:56:04: #2 finished! INFO @ Tue, 16 Jun 2020 07:56:04: #2 predicted fragment length is 128 bps INFO @ Tue, 16 Jun 2020 07:56:04: #2 alternative fragment length(s) may be 128,597 bps INFO @ Tue, 16 Jun 2020 07:56:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20_model.r INFO @ Tue, 16 Jun 2020 07:56:04: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:56:04: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:56:11: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:56:14: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:56:14: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:56:14: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX065633/SRX065633.20_summits.bed INFO @ Tue, 16 Jun 2020 07:56:14: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (208 records, 4 fields): 2 millis CompletedMACS2peakCalling