Job ID = 6366097 SRX = SRX060162 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:27:44 prefetch.2.10.7: 1) Downloading 'SRR192328'... 2020-06-15T22:27:44 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:30:16 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:30:16 prefetch.2.10.7: 1) 'SRR192328' was downloaded successfully Read 30768648 spots for SRR192328/SRR192328.sra Written 30768648 spots for SRR192328/SRR192328.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:20 30768648 reads; of these: 30768648 (100.00%) were unpaired; of these: 896658 (2.91%) aligned 0 times 24719870 (80.34%) aligned exactly 1 time 5152120 (16.74%) aligned >1 times 97.09% overall alignment rate Time searching: 00:05:20 Overall time: 00:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3756802 / 29871990 = 0.1258 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:43:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:43:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:43:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:43:50: 1000000 INFO @ Tue, 16 Jun 2020 07:43:56: 2000000 INFO @ Tue, 16 Jun 2020 07:44:02: 3000000 INFO @ Tue, 16 Jun 2020 07:44:08: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:44:14: 5000000 INFO @ Tue, 16 Jun 2020 07:44:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:44:14: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:44:14: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:44:20: 1000000 INFO @ Tue, 16 Jun 2020 07:44:21: 6000000 INFO @ Tue, 16 Jun 2020 07:44:26: 2000000 INFO @ Tue, 16 Jun 2020 07:44:27: 7000000 INFO @ Tue, 16 Jun 2020 07:44:32: 3000000 INFO @ Tue, 16 Jun 2020 07:44:33: 8000000 INFO @ Tue, 16 Jun 2020 07:44:38: 4000000 INFO @ Tue, 16 Jun 2020 07:44:40: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:44:44: 5000000 INFO @ Tue, 16 Jun 2020 07:44:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:44:44: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:44:44: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:44:46: 10000000 INFO @ Tue, 16 Jun 2020 07:44:50: 6000000 INFO @ Tue, 16 Jun 2020 07:44:51: 1000000 INFO @ Tue, 16 Jun 2020 07:44:53: 11000000 INFO @ Tue, 16 Jun 2020 07:44:56: 7000000 INFO @ Tue, 16 Jun 2020 07:44:58: 2000000 INFO @ Tue, 16 Jun 2020 07:45:00: 12000000 INFO @ Tue, 16 Jun 2020 07:45:02: 8000000 INFO @ Tue, 16 Jun 2020 07:45:04: 3000000 INFO @ Tue, 16 Jun 2020 07:45:07: 13000000 INFO @ Tue, 16 Jun 2020 07:45:08: 9000000 INFO @ Tue, 16 Jun 2020 07:45:11: 4000000 INFO @ Tue, 16 Jun 2020 07:45:13: 14000000 INFO @ Tue, 16 Jun 2020 07:45:14: 10000000 INFO @ Tue, 16 Jun 2020 07:45:17: 5000000 INFO @ Tue, 16 Jun 2020 07:45:20: 15000000 INFO @ Tue, 16 Jun 2020 07:45:20: 11000000 INFO @ Tue, 16 Jun 2020 07:45:24: 6000000 INFO @ Tue, 16 Jun 2020 07:45:26: 12000000 INFO @ Tue, 16 Jun 2020 07:45:27: 16000000 INFO @ Tue, 16 Jun 2020 07:45:30: 7000000 INFO @ Tue, 16 Jun 2020 07:45:32: 13000000 INFO @ Tue, 16 Jun 2020 07:45:33: 17000000 INFO @ Tue, 16 Jun 2020 07:45:37: 8000000 INFO @ Tue, 16 Jun 2020 07:45:38: 14000000 INFO @ Tue, 16 Jun 2020 07:45:40: 18000000 INFO @ Tue, 16 Jun 2020 07:45:44: 9000000 INFO @ Tue, 16 Jun 2020 07:45:44: 15000000 INFO @ Tue, 16 Jun 2020 07:45:46: 19000000 INFO @ Tue, 16 Jun 2020 07:45:50: 16000000 INFO @ Tue, 16 Jun 2020 07:45:50: 10000000 INFO @ Tue, 16 Jun 2020 07:45:53: 20000000 INFO @ Tue, 16 Jun 2020 07:45:56: 17000000 INFO @ Tue, 16 Jun 2020 07:45:57: 11000000 INFO @ Tue, 16 Jun 2020 07:45:59: 21000000 INFO @ Tue, 16 Jun 2020 07:46:02: 18000000 INFO @ Tue, 16 Jun 2020 07:46:03: 12000000 INFO @ Tue, 16 Jun 2020 07:46:06: 22000000 INFO @ Tue, 16 Jun 2020 07:46:08: 19000000 INFO @ Tue, 16 Jun 2020 07:46:10: 13000000 INFO @ Tue, 16 Jun 2020 07:46:12: 23000000 INFO @ Tue, 16 Jun 2020 07:46:14: 20000000 INFO @ Tue, 16 Jun 2020 07:46:16: 14000000 INFO @ Tue, 16 Jun 2020 07:46:19: 24000000 INFO @ Tue, 16 Jun 2020 07:46:20: 21000000 INFO @ Tue, 16 Jun 2020 07:46:22: 15000000 INFO @ Tue, 16 Jun 2020 07:46:25: 25000000 INFO @ Tue, 16 Jun 2020 07:46:26: 22000000 INFO @ Tue, 16 Jun 2020 07:46:29: 16000000 INFO @ Tue, 16 Jun 2020 07:46:31: 26000000 INFO @ Tue, 16 Jun 2020 07:46:31: 23000000 INFO @ Tue, 16 Jun 2020 07:46:32: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:46:32: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:46:32: #1 total tags in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:46:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:46:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:46:33: #1 tags after filtering in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:46:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:46:33: #1 finished! INFO @ Tue, 16 Jun 2020 07:46:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:46:33: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:46:34: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 07:46:34: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:46:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:46:35: 17000000 INFO @ Tue, 16 Jun 2020 07:46:37: 24000000 INFO @ Tue, 16 Jun 2020 07:46:42: 18000000 INFO @ Tue, 16 Jun 2020 07:46:43: 25000000 INFO @ Tue, 16 Jun 2020 07:46:48: 19000000 INFO @ Tue, 16 Jun 2020 07:46:49: 26000000 INFO @ Tue, 16 Jun 2020 07:46:50: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:46:50: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:46:50: #1 total tags in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:46:50: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:46:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:46:51: #1 tags after filtering in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:46:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:46:51: #1 finished! INFO @ Tue, 16 Jun 2020 07:46:51: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:46:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:46:52: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 07:46:52: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:46:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:46:54: 20000000 INFO @ Tue, 16 Jun 2020 07:47:00: 21000000 INFO @ Tue, 16 Jun 2020 07:47:06: 22000000 INFO @ Tue, 16 Jun 2020 07:47:12: 23000000 INFO @ Tue, 16 Jun 2020 07:47:18: 24000000 INFO @ Tue, 16 Jun 2020 07:47:24: 25000000 BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:47:30: 26000000 INFO @ Tue, 16 Jun 2020 07:47:30: #1 tag size is determined as 36 bps INFO @ Tue, 16 Jun 2020 07:47:30: #1 tag size = 36 INFO @ Tue, 16 Jun 2020 07:47:30: #1 total tags in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:47:30: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:47:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:47:31: #1 tags after filtering in treatment: 26115188 INFO @ Tue, 16 Jun 2020 07:47:31: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:47:31: #1 finished! INFO @ Tue, 16 Jun 2020 07:47:31: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:47:31: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:47:32: #2 number of paired peaks: 96 WARNING @ Tue, 16 Jun 2020 07:47:32: Too few paired peaks (96) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:47:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX060162/SRX060162.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling