Job ID = 6366032 SRX = SRX054260 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:29:29 prefetch.2.10.7: 1) Downloading 'SRR164267'... 2020-06-15T22:29:29 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:30:06 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:30:07 prefetch.2.10.7: 'SRR164267' is valid 2020-06-15T22:30:07 prefetch.2.10.7: 1) 'SRR164267' was downloaded successfully Read 4173453 spots for SRR164267/SRR164267.sra Written 4173453 spots for SRR164267/SRR164267.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:20 4173453 reads; of these: 4173453 (100.00%) were unpaired; of these: 3669844 (87.93%) aligned 0 times 443753 (10.63%) aligned exactly 1 time 59856 (1.43%) aligned >1 times 12.07% overall alignment rate Time searching: 00:00:20 Overall time: 00:00:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 10746 / 503609 = 0.0213 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:31:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:31:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:31:21: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:31:25: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:31:25: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:31:25: #1 total tags in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:31:25: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:31:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:31:25: #1 tags after filtering in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:31:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:31:25: #1 finished! INFO @ Tue, 16 Jun 2020 07:31:25: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:31:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:31:25: #2 number of paired peaks: 177 WARNING @ Tue, 16 Jun 2020 07:31:25: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 16 Jun 2020 07:31:25: start model_add_line... INFO @ Tue, 16 Jun 2020 07:31:25: start X-correlation... INFO @ Tue, 16 Jun 2020 07:31:25: end of X-cor INFO @ Tue, 16 Jun 2020 07:31:25: #2 finished! INFO @ Tue, 16 Jun 2020 07:31:25: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 07:31:25: #2 alternative fragment length(s) may be 54,97,128,224,253,295,374,465,526,546,571,586 bps INFO @ Tue, 16 Jun 2020 07:31:25: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05_model.r WARNING @ Tue, 16 Jun 2020 07:31:25: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:31:25: #2 You may need to consider one of the other alternative d(s): 54,97,128,224,253,295,374,465,526,546,571,586 WARNING @ Tue, 16 Jun 2020 07:31:25: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:31:25: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:31:25: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:31:26: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:31:27: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.05_summits.bed INFO @ Tue, 16 Jun 2020 07:31:27: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (17 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:31:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:31:51: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:31:51: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:31:55: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:31:55: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:31:55: #1 total tags in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:31:55: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:31:55: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:31:55: #1 tags after filtering in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:31:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:31:55: #1 finished! INFO @ Tue, 16 Jun 2020 07:31:55: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:31:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:31:55: #2 number of paired peaks: 177 WARNING @ Tue, 16 Jun 2020 07:31:55: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 16 Jun 2020 07:31:55: start model_add_line... INFO @ Tue, 16 Jun 2020 07:31:55: start X-correlation... INFO @ Tue, 16 Jun 2020 07:31:55: end of X-cor INFO @ Tue, 16 Jun 2020 07:31:55: #2 finished! INFO @ Tue, 16 Jun 2020 07:31:55: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 07:31:55: #2 alternative fragment length(s) may be 54,97,128,224,253,295,374,465,526,546,571,586 bps INFO @ Tue, 16 Jun 2020 07:31:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10_model.r WARNING @ Tue, 16 Jun 2020 07:31:55: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:31:55: #2 You may need to consider one of the other alternative d(s): 54,97,128,224,253,295,374,465,526,546,571,586 WARNING @ Tue, 16 Jun 2020 07:31:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:31:55: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:31:55: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:31:56: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:31:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:31:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:31:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.10_summits.bed INFO @ Tue, 16 Jun 2020 07:31:57: Done! pass1 - making usageList (5 chroms): 0 millis pass2 - checking and writing primary data (9 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:32:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:32:21: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:32:21: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:32:24: #1 tag size is determined as 34 bps INFO @ Tue, 16 Jun 2020 07:32:24: #1 tag size = 34 INFO @ Tue, 16 Jun 2020 07:32:24: #1 total tags in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:32:24: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:32:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:32:24: #1 tags after filtering in treatment: 492863 INFO @ Tue, 16 Jun 2020 07:32:24: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:32:24: #1 finished! INFO @ Tue, 16 Jun 2020 07:32:24: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:32:24: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:32:24: #2 number of paired peaks: 177 WARNING @ Tue, 16 Jun 2020 07:32:24: Fewer paired peaks (177) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 177 pairs to build model! INFO @ Tue, 16 Jun 2020 07:32:24: start model_add_line... INFO @ Tue, 16 Jun 2020 07:32:24: start X-correlation... INFO @ Tue, 16 Jun 2020 07:32:24: end of X-cor INFO @ Tue, 16 Jun 2020 07:32:24: #2 finished! INFO @ Tue, 16 Jun 2020 07:32:24: #2 predicted fragment length is 54 bps INFO @ Tue, 16 Jun 2020 07:32:24: #2 alternative fragment length(s) may be 54,97,128,224,253,295,374,465,526,546,571,586 bps INFO @ Tue, 16 Jun 2020 07:32:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20_model.r WARNING @ Tue, 16 Jun 2020 07:32:24: #2 Since the d (54) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:32:24: #2 You may need to consider one of the other alternative d(s): 54,97,128,224,253,295,374,465,526,546,571,586 WARNING @ Tue, 16 Jun 2020 07:32:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:32:24: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:32:24: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:32:26: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:32:26: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:32:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:32:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX054260/SRX054260.20_summits.bed INFO @ Tue, 16 Jun 2020 07:32:26: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling