Job ID = 6365996 SRX = SRX044007 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:36:21 prefetch.2.10.7: 1) Downloading 'SRR107589'... 2020-06-15T22:36:21 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:36:42 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:36:42 prefetch.2.10.7: 'SRR107589' is valid 2020-06-15T22:36:42 prefetch.2.10.7: 1) 'SRR107589' was downloaded successfully Read 3061161 spots for SRR107589/SRR107589.sra Written 3061161 spots for SRR107589/SRR107589.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:19 3061161 reads; of these: 3061161 (100.00%) were unpaired; of these: 2069152 (67.59%) aligned 0 times 835351 (27.29%) aligned exactly 1 time 156658 (5.12%) aligned >1 times 32.41% overall alignment rate Time searching: 00:00:19 Overall time: 00:00:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 23206 / 992009 = 0.0234 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:37:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:37:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:37:57: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:38:03: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:38:03: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:38:03: #1 total tags in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:38:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:38:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:38:03: #1 tags after filtering in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:38:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:38:03: #1 finished! INFO @ Tue, 16 Jun 2020 07:38:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:38:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:38:03: #2 number of paired peaks: 352 WARNING @ Tue, 16 Jun 2020 07:38:03: Fewer paired peaks (352) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 352 pairs to build model! INFO @ Tue, 16 Jun 2020 07:38:03: start model_add_line... INFO @ Tue, 16 Jun 2020 07:38:03: start X-correlation... INFO @ Tue, 16 Jun 2020 07:38:03: end of X-cor INFO @ Tue, 16 Jun 2020 07:38:03: #2 finished! INFO @ Tue, 16 Jun 2020 07:38:03: #2 predicted fragment length is 44 bps INFO @ Tue, 16 Jun 2020 07:38:03: #2 alternative fragment length(s) may be 44,217,447,500,544,571 bps INFO @ Tue, 16 Jun 2020 07:38:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05_model.r WARNING @ Tue, 16 Jun 2020 07:38:03: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:38:03: #2 You may need to consider one of the other alternative d(s): 44,217,447,500,544,571 WARNING @ Tue, 16 Jun 2020 07:38:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:38:03: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:38:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:38:06: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:38:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:38:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:38:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.05_summits.bed INFO @ Tue, 16 Jun 2020 07:38:07: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (102 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:38:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:38:27: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:38:27: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:38:33: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:38:33: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:38:33: #1 total tags in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:38:33: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:38:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:38:33: #1 tags after filtering in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:38:33: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:38:33: #1 finished! INFO @ Tue, 16 Jun 2020 07:38:33: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:38:33: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:38:33: #2 number of paired peaks: 352 WARNING @ Tue, 16 Jun 2020 07:38:33: Fewer paired peaks (352) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 352 pairs to build model! INFO @ Tue, 16 Jun 2020 07:38:33: start model_add_line... INFO @ Tue, 16 Jun 2020 07:38:33: start X-correlation... INFO @ Tue, 16 Jun 2020 07:38:33: end of X-cor INFO @ Tue, 16 Jun 2020 07:38:33: #2 finished! INFO @ Tue, 16 Jun 2020 07:38:33: #2 predicted fragment length is 44 bps INFO @ Tue, 16 Jun 2020 07:38:33: #2 alternative fragment length(s) may be 44,217,447,500,544,571 bps INFO @ Tue, 16 Jun 2020 07:38:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10_model.r WARNING @ Tue, 16 Jun 2020 07:38:33: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:38:33: #2 You may need to consider one of the other alternative d(s): 44,217,447,500,544,571 WARNING @ Tue, 16 Jun 2020 07:38:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:38:33: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:38:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:38:35: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:38:37: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:38:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:38:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.10_summits.bed INFO @ Tue, 16 Jun 2020 07:38:37: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (27 records, 4 fields): 0 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:38:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:38:57: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:38:57: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:39:03: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:39:03: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:39:03: #1 total tags in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:39:03: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:39:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:39:03: #1 tags after filtering in treatment: 968803 INFO @ Tue, 16 Jun 2020 07:39:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:39:03: #1 finished! INFO @ Tue, 16 Jun 2020 07:39:03: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:39:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:39:03: #2 number of paired peaks: 352 WARNING @ Tue, 16 Jun 2020 07:39:03: Fewer paired peaks (352) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 352 pairs to build model! INFO @ Tue, 16 Jun 2020 07:39:03: start model_add_line... INFO @ Tue, 16 Jun 2020 07:39:03: start X-correlation... INFO @ Tue, 16 Jun 2020 07:39:03: end of X-cor INFO @ Tue, 16 Jun 2020 07:39:03: #2 finished! INFO @ Tue, 16 Jun 2020 07:39:03: #2 predicted fragment length is 44 bps INFO @ Tue, 16 Jun 2020 07:39:03: #2 alternative fragment length(s) may be 44,217,447,500,544,571 bps INFO @ Tue, 16 Jun 2020 07:39:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20_model.r WARNING @ Tue, 16 Jun 2020 07:39:03: #2 Since the d (44) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:39:03: #2 You may need to consider one of the other alternative d(s): 44,217,447,500,544,571 WARNING @ Tue, 16 Jun 2020 07:39:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:39:03: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:39:03: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:39:06: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:39:07: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:39:07: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:39:07: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX044007/SRX044007.20_summits.bed INFO @ Tue, 16 Jun 2020 07:39:07: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (6 records, 4 fields): 0 millis CompletedMACS2peakCalling