Job ID = 6365978 SRX = SRX043989 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:37:10 prefetch.2.10.7: 1) Downloading 'SRR107564'... 2020-06-15T22:37:10 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:37:56 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:37:56 prefetch.2.10.7: 'SRR107564' is valid 2020-06-15T22:37:56 prefetch.2.10.7: 1) 'SRR107564' was downloaded successfully Read 5494920 spots for SRR107564/SRR107564.sra Written 5494920 spots for SRR107564/SRR107564.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:44 5494920 reads; of these: 5494920 (100.00%) were unpaired; of these: 1730665 (31.50%) aligned 0 times 3077952 (56.01%) aligned exactly 1 time 686303 (12.49%) aligned >1 times 68.50% overall alignment rate Time searching: 00:00:44 Overall time: 00:00:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 369620 / 3764255 = 0.0982 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:40:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:40:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:40:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:40:22: 1000000 INFO @ Tue, 16 Jun 2020 07:40:27: 2000000 INFO @ Tue, 16 Jun 2020 07:40:32: 3000000 INFO @ Tue, 16 Jun 2020 07:40:34: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:40:34: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:40:34: #1 total tags in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:40:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:40:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:40:34: #1 tags after filtering in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:40:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:40:34: #1 finished! INFO @ Tue, 16 Jun 2020 07:40:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:40:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:40:34: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 07:40:34: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 07:40:34: start model_add_line... INFO @ Tue, 16 Jun 2020 07:40:34: start X-correlation... INFO @ Tue, 16 Jun 2020 07:40:34: end of X-cor INFO @ Tue, 16 Jun 2020 07:40:34: #2 finished! INFO @ Tue, 16 Jun 2020 07:40:34: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 07:40:34: #2 alternative fragment length(s) may be 3,31,433,511,562,591,595 bps INFO @ Tue, 16 Jun 2020 07:40:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05_model.r WARNING @ Tue, 16 Jun 2020 07:40:34: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:40:34: #2 You may need to consider one of the other alternative d(s): 3,31,433,511,562,591,595 WARNING @ Tue, 16 Jun 2020 07:40:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:40:34: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:40:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:40:42: #3 Call peaks for each chromosome... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:40:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05_peaks.xls INFO @ Tue, 16 Jun 2020 07:40:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:40:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.05_summits.bed INFO @ Tue, 16 Jun 2020 07:40:46: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (425 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:40:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:40:47: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:40:47: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:40:52: 1000000 INFO @ Tue, 16 Jun 2020 07:40:57: 2000000 INFO @ Tue, 16 Jun 2020 07:41:02: 3000000 INFO @ Tue, 16 Jun 2020 07:41:04: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:41:04: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:41:04: #1 total tags in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:41:04: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:41:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:41:04: #1 tags after filtering in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:41:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:41:04: #1 finished! INFO @ Tue, 16 Jun 2020 07:41:04: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:41:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:41:04: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 07:41:04: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 07:41:04: start model_add_line... INFO @ Tue, 16 Jun 2020 07:41:04: start X-correlation... INFO @ Tue, 16 Jun 2020 07:41:04: end of X-cor INFO @ Tue, 16 Jun 2020 07:41:04: #2 finished! INFO @ Tue, 16 Jun 2020 07:41:04: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 07:41:04: #2 alternative fragment length(s) may be 3,31,433,511,562,591,595 bps INFO @ Tue, 16 Jun 2020 07:41:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10_model.r WARNING @ Tue, 16 Jun 2020 07:41:04: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:41:04: #2 You may need to consider one of the other alternative d(s): 3,31,433,511,562,591,595 WARNING @ Tue, 16 Jun 2020 07:41:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:41:04: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:41:04: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 16 Jun 2020 07:41:12: #3 Call peaks for each chromosome... BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:41:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10_peaks.xls INFO @ Tue, 16 Jun 2020 07:41:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:41:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.10_summits.bed INFO @ Tue, 16 Jun 2020 07:41:16: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (189 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Tue, 16 Jun 2020 07:41:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:41:17: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:41:17: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:41:22: 1000000 INFO @ Tue, 16 Jun 2020 07:41:27: 2000000 INFO @ Tue, 16 Jun 2020 07:41:32: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 16 Jun 2020 07:41:34: #1 tag size is determined as 32 bps INFO @ Tue, 16 Jun 2020 07:41:34: #1 tag size = 32 INFO @ Tue, 16 Jun 2020 07:41:34: #1 total tags in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:41:34: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:41:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:41:34: #1 tags after filtering in treatment: 3394635 INFO @ Tue, 16 Jun 2020 07:41:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 16 Jun 2020 07:41:34: #1 finished! INFO @ Tue, 16 Jun 2020 07:41:34: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:41:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:41:35: #2 number of paired peaks: 451 WARNING @ Tue, 16 Jun 2020 07:41:35: Fewer paired peaks (451) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 451 pairs to build model! INFO @ Tue, 16 Jun 2020 07:41:35: start model_add_line... INFO @ Tue, 16 Jun 2020 07:41:35: start X-correlation... INFO @ Tue, 16 Jun 2020 07:41:35: end of X-cor INFO @ Tue, 16 Jun 2020 07:41:35: #2 finished! INFO @ Tue, 16 Jun 2020 07:41:35: #2 predicted fragment length is 31 bps INFO @ Tue, 16 Jun 2020 07:41:35: #2 alternative fragment length(s) may be 3,31,433,511,562,591,595 bps INFO @ Tue, 16 Jun 2020 07:41:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20_model.r WARNING @ Tue, 16 Jun 2020 07:41:35: #2 Since the d (31) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 16 Jun 2020 07:41:35: #2 You may need to consider one of the other alternative d(s): 3,31,433,511,562,591,595 WARNING @ Tue, 16 Jun 2020 07:41:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 16 Jun 2020 07:41:35: #3 Call peaks... INFO @ Tue, 16 Jun 2020 07:41:35: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:41:42: #3 Call peaks for each chromosome... INFO @ Tue, 16 Jun 2020 07:41:46: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20_peaks.xls INFO @ Tue, 16 Jun 2020 07:41:46: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20_peaks.narrowPeak INFO @ Tue, 16 Jun 2020 07:41:46: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/SRX043989/SRX043989.20_summits.bed INFO @ Tue, 16 Jun 2020 07:41:46: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (47 records, 4 fields): 2 millis CompletedMACS2peakCalling