Job ID = 6365904 SRX = SRX015102 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T23:31:50 prefetch.2.10.7: 1) Downloading 'SRR032448'... 2020-06-15T23:31:50 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T23:31:58 prefetch.2.10.7: HTTPS download succeed 2020-06-15T23:31:58 prefetch.2.10.7: 'SRR032448' is valid 2020-06-15T23:31:58 prefetch.2.10.7: 1) 'SRR032448' was downloaded successfully Read 19233 spots for SRR032448/SRR032448.sra Written 19233 spots for SRR032448/SRR032448.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:01 19233 reads; of these: 19233 (100.00%) were unpaired; of these: 19229 (99.98%) aligned 0 times 1 (0.01%) aligned exactly 1 time 3 (0.02%) aligned >1 times 0.02% overall alignment rate Time searching: 00:00:01 Overall time: 00:00:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 0 / 4 = 0.0000 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:32:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:32:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:32:32: #1 read treatment tags... Exception struct.error: 'unpack requires a string argument of length 4' in 'MACS2.IO.Parser.BAMParser.tsize' ignored INFO @ Tue, 16 Jun 2020 08:32:32: #1 tag size is determined as 0 bps INFO @ Tue, 16 Jun 2020 08:32:32: #1 tag size = 0 INFO @ Tue, 16 Jun 2020 08:32:32: #1 total tags in treatment: 4 INFO @ Tue, 16 Jun 2020 08:32:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:32:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:32:32: #1 tags after filtering in treatment: 0 INFO @ Tue, 16 Jun 2020 08:32:32: #1 Redundant rate of treatment: 1.00 INFO @ Tue, 16 Jun 2020 08:32:32: #1 finished! INFO @ Tue, 16 Jun 2020 08:32:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:32:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:32:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:32:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:32:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 08:33:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:02: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:02: #1 read treatment tags... Exception struct.error: 'unpack requires a string argument of length 4' in 'MACS2.IO.Parser.BAMParser.tsize' ignored INFO @ Tue, 16 Jun 2020 08:33:02: #1 tag size is determined as 0 bps INFO @ Tue, 16 Jun 2020 08:33:02: #1 tag size = 0 INFO @ Tue, 16 Jun 2020 08:33:02: #1 total tags in treatment: 4 INFO @ Tue, 16 Jun 2020 08:33:02: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:02: #1 tags after filtering in treatment: 0 INFO @ Tue, 16 Jun 2020 08:33:02: #1 Redundant rate of treatment: 1.00 INFO @ Tue, 16 Jun 2020 08:33:02: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:02: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:02: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:33:02: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:02: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 08:33:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 08:33:32: #1 read tag files... INFO @ Tue, 16 Jun 2020 08:33:32: #1 read treatment tags... Exception struct.error: 'unpack requires a string argument of length 4' in 'MACS2.IO.Parser.BAMParser.tsize' ignored INFO @ Tue, 16 Jun 2020 08:33:32: #1 tag size is determined as 0 bps INFO @ Tue, 16 Jun 2020 08:33:32: #1 tag size = 0 INFO @ Tue, 16 Jun 2020 08:33:32: #1 total tags in treatment: 4 INFO @ Tue, 16 Jun 2020 08:33:32: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 08:33:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 08:33:32: #1 tags after filtering in treatment: 0 INFO @ Tue, 16 Jun 2020 08:33:32: #1 Redundant rate of treatment: 1.00 INFO @ Tue, 16 Jun 2020 08:33:32: #1 finished! INFO @ Tue, 16 Jun 2020 08:33:32: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 08:33:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 08:33:32: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 08:33:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 08:33:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015102/SRX015102.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling