Job ID = 6365902 SRX = SRX015100 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-15T22:24:59 prefetch.2.10.7: 1) Downloading 'SRR032446'... 2020-06-15T22:24:59 prefetch.2.10.7: Downloading via HTTPS... 2020-06-15T22:25:09 prefetch.2.10.7: HTTPS download succeed 2020-06-15T22:25:09 prefetch.2.10.7: 'SRR032446' is valid 2020-06-15T22:25:09 prefetch.2.10.7: 1) 'SRR032446' was downloaded successfully Read 25901 spots for SRR032446/SRR032446.sra Written 25901 spots for SRR032446/SRR032446.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:00 25901 reads; of these: 25901 (100.00%) were unpaired; of these: 25868 (99.87%) aligned 0 times 9 (0.03%) aligned exactly 1 time 24 (0.09%) aligned >1 times 0.13% overall alignment rate Time searching: 00:00:00 Overall time: 00:00:00 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 6 / 33 = 0.1818 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:25:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:25:42: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:25:42: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:25:42: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:25:42: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:25:42: #1 total tags in treatment: 27 INFO @ Tue, 16 Jun 2020 07:25:42: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:25:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:25:42: #1 tags after filtering in treatment: 24 INFO @ Tue, 16 Jun 2020 07:25:42: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 07:25:42: #1 finished! INFO @ Tue, 16 Jun 2020 07:25:42: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:25:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:25:42: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 07:25:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:25:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 16 Jun 2020 07:26:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:26:12: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:26:12: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:26:12: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:26:12: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:26:12: #1 total tags in treatment: 27 INFO @ Tue, 16 Jun 2020 07:26:12: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:26:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:26:12: #1 tags after filtering in treatment: 24 INFO @ Tue, 16 Jun 2020 07:26:12: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 07:26:12: #1 finished! INFO @ Tue, 16 Jun 2020 07:26:12: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:26:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:26:12: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 07:26:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:26:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Tue, 16 Jun 2020 07:26:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 16 Jun 2020 07:26:43: #1 read tag files... INFO @ Tue, 16 Jun 2020 07:26:43: #1 read treatment tags... INFO @ Tue, 16 Jun 2020 07:26:43: #1 tag size is determined as 27 bps INFO @ Tue, 16 Jun 2020 07:26:43: #1 tag size = 27 INFO @ Tue, 16 Jun 2020 07:26:43: #1 total tags in treatment: 27 INFO @ Tue, 16 Jun 2020 07:26:43: #1 user defined the maximum tags... INFO @ Tue, 16 Jun 2020 07:26:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 16 Jun 2020 07:26:43: #1 tags after filtering in treatment: 24 INFO @ Tue, 16 Jun 2020 07:26:43: #1 Redundant rate of treatment: 0.11 INFO @ Tue, 16 Jun 2020 07:26:43: #1 finished! INFO @ Tue, 16 Jun 2020 07:26:43: #2 Build Peak Model... INFO @ Tue, 16 Jun 2020 07:26:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 16 Jun 2020 07:26:43: #2 number of paired peaks: 0 WARNING @ Tue, 16 Jun 2020 07:26:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 16 Jun 2020 07:26:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce11/SRX015100/SRX015100.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling