Job ID = 14159115 SRX = DRX184551 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:06 37754672 reads; of these: 37754672 (100.00%) were unpaired; of these: 7401441 (19.60%) aligned 0 times 20549265 (54.43%) aligned exactly 1 time 9803966 (25.97%) aligned >1 times 80.40% overall alignment rate Time searching: 00:08:06 Overall time: 00:08:06 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13753081 / 30353231 = 0.4531 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:53:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:53:39: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:53:39: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:53:44: 1000000 INFO @ Wed, 08 Dec 2021 19:53:50: 2000000 INFO @ Wed, 08 Dec 2021 19:53:55: 3000000 INFO @ Wed, 08 Dec 2021 19:54:00: 4000000 INFO @ Wed, 08 Dec 2021 19:54:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:54:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:54:09: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:54:09: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:54:10: 6000000 INFO @ Wed, 08 Dec 2021 19:54:16: 1000000 INFO @ Wed, 08 Dec 2021 19:54:17: 7000000 INFO @ Wed, 08 Dec 2021 19:54:23: 2000000 INFO @ Wed, 08 Dec 2021 19:54:23: 8000000 INFO @ Wed, 08 Dec 2021 19:54:29: 9000000 INFO @ Wed, 08 Dec 2021 19:54:29: 3000000 INFO @ Wed, 08 Dec 2021 19:54:35: 10000000 INFO @ Wed, 08 Dec 2021 19:54:35: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:54:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:54:39: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:54:39: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:54:42: 5000000 INFO @ Wed, 08 Dec 2021 19:54:42: 11000000 INFO @ Wed, 08 Dec 2021 19:54:46: 1000000 INFO @ Wed, 08 Dec 2021 19:54:48: 6000000 INFO @ Wed, 08 Dec 2021 19:54:48: 12000000 INFO @ Wed, 08 Dec 2021 19:54:54: 2000000 INFO @ Wed, 08 Dec 2021 19:54:54: 13000000 INFO @ Wed, 08 Dec 2021 19:54:55: 7000000 INFO @ Wed, 08 Dec 2021 19:55:01: 14000000 INFO @ Wed, 08 Dec 2021 19:55:01: 3000000 INFO @ Wed, 08 Dec 2021 19:55:01: 8000000 INFO @ Wed, 08 Dec 2021 19:55:07: 15000000 INFO @ Wed, 08 Dec 2021 19:55:08: 9000000 INFO @ Wed, 08 Dec 2021 19:55:08: 4000000 INFO @ Wed, 08 Dec 2021 19:55:13: 16000000 INFO @ Wed, 08 Dec 2021 19:55:14: 10000000 INFO @ Wed, 08 Dec 2021 19:55:15: 5000000 INFO @ Wed, 08 Dec 2021 19:55:17: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:55:17: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:55:17: #1 total tags in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:55:17: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:55:17: #1 tags after filtering in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:55:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:55:17: #1 finished! INFO @ Wed, 08 Dec 2021 19:55:17: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:55:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:55:19: #2 number of paired peaks: 1974 INFO @ Wed, 08 Dec 2021 19:55:19: start model_add_line... INFO @ Wed, 08 Dec 2021 19:55:19: start X-correlation... INFO @ Wed, 08 Dec 2021 19:55:19: end of X-cor INFO @ Wed, 08 Dec 2021 19:55:19: #2 finished! INFO @ Wed, 08 Dec 2021 19:55:19: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 19:55:19: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 19:55:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05_model.r INFO @ Wed, 08 Dec 2021 19:55:19: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:55:19: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:55:21: 11000000 INFO @ Wed, 08 Dec 2021 19:55:22: 6000000 INFO @ Wed, 08 Dec 2021 19:55:27: 12000000 INFO @ Wed, 08 Dec 2021 19:55:30: 7000000 INFO @ Wed, 08 Dec 2021 19:55:34: 13000000 INFO @ Wed, 08 Dec 2021 19:55:37: 8000000 INFO @ Wed, 08 Dec 2021 19:55:40: 14000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 19:55:44: 9000000 INFO @ Wed, 08 Dec 2021 19:55:47: 15000000 INFO @ Wed, 08 Dec 2021 19:55:51: 10000000 INFO @ Wed, 08 Dec 2021 19:55:53: 16000000 INFO @ Wed, 08 Dec 2021 19:55:57: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:55:57: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:55:57: #1 total tags in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:55:57: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:55:57: #1 tags after filtering in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:55:57: #1 finished! INFO @ Wed, 08 Dec 2021 19:55:57: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:55:58: 11000000 INFO @ Wed, 08 Dec 2021 19:55:59: #2 number of paired peaks: 1974 INFO @ Wed, 08 Dec 2021 19:55:59: start model_add_line... INFO @ Wed, 08 Dec 2021 19:55:59: start X-correlation... INFO @ Wed, 08 Dec 2021 19:55:59: end of X-cor INFO @ Wed, 08 Dec 2021 19:55:59: #2 finished! INFO @ Wed, 08 Dec 2021 19:55:59: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 19:55:59: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 19:55:59: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10_model.r INFO @ Wed, 08 Dec 2021 19:55:59: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:55:59: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:55:59: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:56:05: 12000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 19:56:12: 13000000 INFO @ Wed, 08 Dec 2021 19:56:16: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05_peaks.xls INFO @ Wed, 08 Dec 2021 19:56:16: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:56:16: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.05_summits.bed INFO @ Wed, 08 Dec 2021 19:56:16: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (19518 records, 4 fields): 95 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:56:18: 14000000 INFO @ Wed, 08 Dec 2021 19:56:25: 15000000 INFO @ Wed, 08 Dec 2021 19:56:31: 16000000 INFO @ Wed, 08 Dec 2021 19:56:35: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:56:35: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:56:35: #1 total tags in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:56:35: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:56:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:56:35: #1 tags after filtering in treatment: 16600150 INFO @ Wed, 08 Dec 2021 19:56:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:56:35: #1 finished! INFO @ Wed, 08 Dec 2021 19:56:35: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:56:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:56:37: #2 number of paired peaks: 1974 INFO @ Wed, 08 Dec 2021 19:56:37: start model_add_line... INFO @ Wed, 08 Dec 2021 19:56:37: start X-correlation... INFO @ Wed, 08 Dec 2021 19:56:37: end of X-cor INFO @ Wed, 08 Dec 2021 19:56:37: #2 finished! INFO @ Wed, 08 Dec 2021 19:56:37: #2 predicted fragment length is 192 bps INFO @ Wed, 08 Dec 2021 19:56:37: #2 alternative fragment length(s) may be 192 bps INFO @ Wed, 08 Dec 2021 19:56:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20_model.r INFO @ Wed, 08 Dec 2021 19:56:37: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:56:37: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:56:41: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:56:58: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10_peaks.xls INFO @ Wed, 08 Dec 2021 19:56:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:56:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.10_summits.bed INFO @ Wed, 08 Dec 2021 19:56:59: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (16993 records, 4 fields): 17 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:57:17: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:57:32: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20_peaks.xls INFO @ Wed, 08 Dec 2021 19:57:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:57:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184551/DRX184551.20_summits.bed INFO @ Wed, 08 Dec 2021 19:57:33: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (13070 records, 4 fields): 13 millis CompletedMACS2peakCalling