Job ID = 14159097 SRX = DRX184550 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:18 23827690 reads; of these: 23827690 (100.00%) were unpaired; of these: 4196293 (17.61%) aligned 0 times 14980937 (62.87%) aligned exactly 1 time 4650460 (19.52%) aligned >1 times 82.39% overall alignment rate Time searching: 00:06:18 Overall time: 00:06:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6741658 / 19631397 = 0.3434 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:30:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:30:18: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:30:18: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:30:27: 1000000 INFO @ Wed, 08 Dec 2021 19:30:34: 2000000 INFO @ Wed, 08 Dec 2021 19:30:41: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:30:48: 4000000 INFO @ Wed, 08 Dec 2021 19:30:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:30:48: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:30:48: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:30:55: 5000000 INFO @ Wed, 08 Dec 2021 19:30:56: 1000000 INFO @ Wed, 08 Dec 2021 19:31:03: 6000000 INFO @ Wed, 08 Dec 2021 19:31:03: 2000000 INFO @ Wed, 08 Dec 2021 19:31:10: 7000000 INFO @ Wed, 08 Dec 2021 19:31:10: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:31:17: 8000000 INFO @ Wed, 08 Dec 2021 19:31:17: 4000000 INFO @ Wed, 08 Dec 2021 19:31:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:31:18: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:31:18: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:31:24: 9000000 INFO @ Wed, 08 Dec 2021 19:31:25: 5000000 INFO @ Wed, 08 Dec 2021 19:31:28: 1000000 INFO @ Wed, 08 Dec 2021 19:31:32: 10000000 INFO @ Wed, 08 Dec 2021 19:31:32: 6000000 INFO @ Wed, 08 Dec 2021 19:31:37: 2000000 INFO @ Wed, 08 Dec 2021 19:31:39: 7000000 INFO @ Wed, 08 Dec 2021 19:31:39: 11000000 INFO @ Wed, 08 Dec 2021 19:31:46: 3000000 INFO @ Wed, 08 Dec 2021 19:31:46: 8000000 INFO @ Wed, 08 Dec 2021 19:31:47: 12000000 INFO @ Wed, 08 Dec 2021 19:31:53: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:31:53: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:31:53: #1 total tags in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:31:53: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:31:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:31:53: #1 tags after filtering in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:31:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:31:53: #1 finished! INFO @ Wed, 08 Dec 2021 19:31:53: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:31:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:31:54: 9000000 INFO @ Wed, 08 Dec 2021 19:31:55: #2 number of paired peaks: 1664 INFO @ Wed, 08 Dec 2021 19:31:55: start model_add_line... INFO @ Wed, 08 Dec 2021 19:31:55: start X-correlation... INFO @ Wed, 08 Dec 2021 19:31:55: end of X-cor INFO @ Wed, 08 Dec 2021 19:31:55: #2 finished! INFO @ Wed, 08 Dec 2021 19:31:55: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 19:31:55: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 19:31:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05_model.r INFO @ Wed, 08 Dec 2021 19:31:55: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:31:55: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:31:56: 4000000 INFO @ Wed, 08 Dec 2021 19:32:01: 10000000 INFO @ Wed, 08 Dec 2021 19:32:05: 5000000 INFO @ Wed, 08 Dec 2021 19:32:08: 11000000 INFO @ Wed, 08 Dec 2021 19:32:14: 6000000 INFO @ Wed, 08 Dec 2021 19:32:15: 12000000 INFO @ Wed, 08 Dec 2021 19:32:22: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:32:22: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:32:22: #1 total tags in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:32:22: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:32:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:32:22: #1 tags after filtering in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:32:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:32:22: #1 finished! INFO @ Wed, 08 Dec 2021 19:32:22: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:32:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:32:23: 7000000 INFO @ Wed, 08 Dec 2021 19:32:23: #2 number of paired peaks: 1664 INFO @ Wed, 08 Dec 2021 19:32:23: start model_add_line... INFO @ Wed, 08 Dec 2021 19:32:24: start X-correlation... INFO @ Wed, 08 Dec 2021 19:32:24: end of X-cor INFO @ Wed, 08 Dec 2021 19:32:24: #2 finished! INFO @ Wed, 08 Dec 2021 19:32:24: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 19:32:24: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 19:32:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10_model.r INFO @ Wed, 08 Dec 2021 19:32:24: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:32:24: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:32:32: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 19:32:41: 9000000 INFO @ Wed, 08 Dec 2021 19:32:44: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:32:50: 10000000 INFO @ Wed, 08 Dec 2021 19:32:59: 11000000 INFO @ Wed, 08 Dec 2021 19:33:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05_peaks.xls INFO @ Wed, 08 Dec 2021 19:33:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:33:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.05_summits.bed INFO @ Wed, 08 Dec 2021 19:33:02: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (7333 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:33:08: 12000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 19:33:12: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:33:15: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:33:15: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:33:15: #1 total tags in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:33:15: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:33:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:33:16: #1 tags after filtering in treatment: 12889739 INFO @ Wed, 08 Dec 2021 19:33:16: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:33:16: #1 finished! INFO @ Wed, 08 Dec 2021 19:33:16: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:33:16: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:33:17: #2 number of paired peaks: 1664 INFO @ Wed, 08 Dec 2021 19:33:17: start model_add_line... INFO @ Wed, 08 Dec 2021 19:33:17: start X-correlation... INFO @ Wed, 08 Dec 2021 19:33:17: end of X-cor INFO @ Wed, 08 Dec 2021 19:33:17: #2 finished! INFO @ Wed, 08 Dec 2021 19:33:17: #2 predicted fragment length is 304 bps INFO @ Wed, 08 Dec 2021 19:33:17: #2 alternative fragment length(s) may be 304 bps INFO @ Wed, 08 Dec 2021 19:33:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20_model.r INFO @ Wed, 08 Dec 2021 19:33:17: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:33:17: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:33:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10_peaks.xls INFO @ Wed, 08 Dec 2021 19:33:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:33:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.10_summits.bed INFO @ Wed, 08 Dec 2021 19:33:30: Done! pass1 - making usageList (7 chroms): 2 millis pass2 - checking and writing primary data (5161 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:34:06: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:34:24: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20_peaks.xls INFO @ Wed, 08 Dec 2021 19:34:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:34:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184550/DRX184550.20_summits.bed INFO @ Wed, 08 Dec 2021 19:34:24: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3601 records, 4 fields): 31 millis CompletedMACS2peakCalling