Job ID = 14159119 SRX = DRX184549 Genome = ce11 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:18 26291984 reads; of these: 26291984 (100.00%) were unpaired; of these: 3287407 (12.50%) aligned 0 times 16376316 (62.29%) aligned exactly 1 time 6628261 (25.21%) aligned >1 times 87.50% overall alignment rate Time searching: 00:06:18 Overall time: 00:06:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 7846210 / 23004577 = 0.3411 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:51:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:51:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:51:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:51:38: 1000000 INFO @ Wed, 08 Dec 2021 19:51:43: 2000000 INFO @ Wed, 08 Dec 2021 19:51:48: 3000000 INFO @ Wed, 08 Dec 2021 19:51:53: 4000000 INFO @ Wed, 08 Dec 2021 19:51:59: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:52:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:52:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:52:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:52:04: 6000000 INFO @ Wed, 08 Dec 2021 19:52:08: 1000000 INFO @ Wed, 08 Dec 2021 19:52:10: 7000000 INFO @ Wed, 08 Dec 2021 19:52:14: 2000000 INFO @ Wed, 08 Dec 2021 19:52:16: 8000000 INFO @ Wed, 08 Dec 2021 19:52:20: 3000000 INFO @ Wed, 08 Dec 2021 19:52:22: 9000000 INFO @ Wed, 08 Dec 2021 19:52:26: 4000000 INFO @ Wed, 08 Dec 2021 19:52:27: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 19:52:32: 5000000 INFO @ Wed, 08 Dec 2021 19:52:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 19:52:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 19:52:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 19:52:33: 11000000 INFO @ Wed, 08 Dec 2021 19:52:38: 6000000 INFO @ Wed, 08 Dec 2021 19:52:39: 12000000 INFO @ Wed, 08 Dec 2021 19:52:39: 1000000 INFO @ Wed, 08 Dec 2021 19:52:44: 7000000 INFO @ Wed, 08 Dec 2021 19:52:45: 13000000 INFO @ Wed, 08 Dec 2021 19:52:46: 2000000 INFO @ Wed, 08 Dec 2021 19:52:51: 8000000 INFO @ Wed, 08 Dec 2021 19:52:52: 14000000 INFO @ Wed, 08 Dec 2021 19:52:53: 3000000 INFO @ Wed, 08 Dec 2021 19:52:57: 9000000 INFO @ Wed, 08 Dec 2021 19:52:58: 15000000 INFO @ Wed, 08 Dec 2021 19:52:59: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:52:59: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:52:59: #1 total tags in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:52:59: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:52:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:52:59: #1 tags after filtering in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:52:59: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:52:59: #1 finished! INFO @ Wed, 08 Dec 2021 19:52:59: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:52:59: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:53:00: #2 number of paired peaks: 1153 INFO @ Wed, 08 Dec 2021 19:53:00: start model_add_line... INFO @ Wed, 08 Dec 2021 19:53:00: start X-correlation... INFO @ Wed, 08 Dec 2021 19:53:00: end of X-cor INFO @ Wed, 08 Dec 2021 19:53:00: #2 finished! INFO @ Wed, 08 Dec 2021 19:53:00: #2 predicted fragment length is 238 bps INFO @ Wed, 08 Dec 2021 19:53:00: #2 alternative fragment length(s) may be 238 bps INFO @ Wed, 08 Dec 2021 19:53:00: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05_model.r INFO @ Wed, 08 Dec 2021 19:53:00: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:53:00: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:53:00: 4000000 INFO @ Wed, 08 Dec 2021 19:53:03: 10000000 INFO @ Wed, 08 Dec 2021 19:53:08: 5000000 INFO @ Wed, 08 Dec 2021 19:53:09: 11000000 INFO @ Wed, 08 Dec 2021 19:53:14: 6000000 INFO @ Wed, 08 Dec 2021 19:53:15: 12000000 INFO @ Wed, 08 Dec 2021 19:53:21: 7000000 INFO @ Wed, 08 Dec 2021 19:53:21: 13000000 INFO @ Wed, 08 Dec 2021 19:53:27: 14000000 INFO @ Wed, 08 Dec 2021 19:53:28: 8000000 INFO @ Wed, 08 Dec 2021 19:53:33: 15000000 INFO @ Wed, 08 Dec 2021 19:53:34: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:53:34: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:53:34: #1 total tags in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:53:34: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:53:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 19:53:35: #1 tags after filtering in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:53:35: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:53:35: #1 finished! INFO @ Wed, 08 Dec 2021 19:53:35: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:53:35: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:53:35: 9000000 INFO @ Wed, 08 Dec 2021 19:53:36: #2 number of paired peaks: 1153 INFO @ Wed, 08 Dec 2021 19:53:36: start model_add_line... INFO @ Wed, 08 Dec 2021 19:53:36: start X-correlation... INFO @ Wed, 08 Dec 2021 19:53:36: end of X-cor INFO @ Wed, 08 Dec 2021 19:53:36: #2 finished! INFO @ Wed, 08 Dec 2021 19:53:36: #2 predicted fragment length is 238 bps INFO @ Wed, 08 Dec 2021 19:53:36: #2 alternative fragment length(s) may be 238 bps INFO @ Wed, 08 Dec 2021 19:53:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10_model.r INFO @ Wed, 08 Dec 2021 19:53:36: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:53:36: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:53:39: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:53:41: 10000000 INFO @ Wed, 08 Dec 2021 19:53:47: 11000000 INFO @ Wed, 08 Dec 2021 19:53:53: 12000000 INFO @ Wed, 08 Dec 2021 19:53:55: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05_peaks.xls INFO @ Wed, 08 Dec 2021 19:53:55: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:53:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.05_summits.bed INFO @ Wed, 08 Dec 2021 19:53:56: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (9064 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:54:00: 13000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 19:54:06: 14000000 INFO @ Wed, 08 Dec 2021 19:54:12: 15000000 INFO @ Wed, 08 Dec 2021 19:54:13: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 19:54:13: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 19:54:13: #1 total tags in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:54:13: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 19:54:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 19:54:13: #1 tags after filtering in treatment: 15158367 INFO @ Wed, 08 Dec 2021 19:54:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 19:54:13: #1 finished! INFO @ Wed, 08 Dec 2021 19:54:13: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 19:54:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 19:54:14: #2 number of paired peaks: 1153 INFO @ Wed, 08 Dec 2021 19:54:14: start model_add_line... INFO @ Wed, 08 Dec 2021 19:54:14: start X-correlation... INFO @ Wed, 08 Dec 2021 19:54:14: end of X-cor INFO @ Wed, 08 Dec 2021 19:54:14: #2 finished! INFO @ Wed, 08 Dec 2021 19:54:14: #2 predicted fragment length is 238 bps INFO @ Wed, 08 Dec 2021 19:54:14: #2 alternative fragment length(s) may be 238 bps INFO @ Wed, 08 Dec 2021 19:54:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20_model.r INFO @ Wed, 08 Dec 2021 19:54:14: #3 Call peaks... INFO @ Wed, 08 Dec 2021 19:54:14: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 19:54:15: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:54:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10_peaks.xls INFO @ Wed, 08 Dec 2021 19:54:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:54:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.10_summits.bed INFO @ Wed, 08 Dec 2021 19:54:31: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (6996 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 19:54:54: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 19:55:10: #4 Write output xls file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20_peaks.xls INFO @ Wed, 08 Dec 2021 19:55:10: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 19:55:10: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce11/DRX184549/DRX184549.20_summits.bed INFO @ Wed, 08 Dec 2021 19:55:10: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (4571 records, 4 fields): 182 millis CompletedMACS2peakCalling