Job ID = 10165774 SRX = SRX9120610 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42619141 spots for SRR12638553/SRR12638553.sra Written 42619141 spots for SRR12638553/SRR12638553.sra fastq に変換しました。 bowtie でマッピング中... Your job 10166130 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:16 42619141 reads; of these: 42619141 (100.00%) were unpaired; of these: 4682240 (10.99%) aligned 0 times 31268135 (73.37%) aligned exactly 1 time 6668766 (15.65%) aligned >1 times 89.01% overall alignment rate Time searching: 00:09:16 Overall time: 00:09:16 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 7557388 / 37936901 = 0.1992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:10:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:10:13: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:10:13: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:10:22: 1000000 INFO @ Thu, 08 Oct 2020 20:10:30: 2000000 INFO @ Thu, 08 Oct 2020 20:10:39: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:10:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:10:43: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:10:43: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:10:47: 4000000 INFO @ Thu, 08 Oct 2020 20:10:51: 1000000 INFO @ Thu, 08 Oct 2020 20:10:56: 5000000 INFO @ Thu, 08 Oct 2020 20:10:59: 2000000 INFO @ Thu, 08 Oct 2020 20:11:04: 6000000 INFO @ Thu, 08 Oct 2020 20:11:07: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:11:12: 7000000 INFO @ Thu, 08 Oct 2020 20:11:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:11:13: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:11:13: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:11:15: 4000000 INFO @ Thu, 08 Oct 2020 20:11:21: 8000000 INFO @ Thu, 08 Oct 2020 20:11:21: 1000000 INFO @ Thu, 08 Oct 2020 20:11:23: 5000000 INFO @ Thu, 08 Oct 2020 20:11:29: 9000000 INFO @ Thu, 08 Oct 2020 20:11:29: 2000000 INFO @ Thu, 08 Oct 2020 20:11:31: 6000000 INFO @ Thu, 08 Oct 2020 20:11:37: 10000000 INFO @ Thu, 08 Oct 2020 20:11:37: 3000000 INFO @ Thu, 08 Oct 2020 20:11:39: 7000000 INFO @ Thu, 08 Oct 2020 20:11:45: 11000000 INFO @ Thu, 08 Oct 2020 20:11:45: 4000000 INFO @ Thu, 08 Oct 2020 20:11:48: 8000000 INFO @ Thu, 08 Oct 2020 20:11:53: 5000000 INFO @ Thu, 08 Oct 2020 20:11:53: 12000000 INFO @ Thu, 08 Oct 2020 20:11:55: 9000000 INFO @ Thu, 08 Oct 2020 20:12:01: 6000000 INFO @ Thu, 08 Oct 2020 20:12:02: 13000000 INFO @ Thu, 08 Oct 2020 20:12:03: 10000000 INFO @ Thu, 08 Oct 2020 20:12:10: 7000000 INFO @ Thu, 08 Oct 2020 20:12:10: 14000000 INFO @ Thu, 08 Oct 2020 20:12:11: 11000000 INFO @ Thu, 08 Oct 2020 20:12:17: 8000000 INFO @ Thu, 08 Oct 2020 20:12:18: 15000000 INFO @ Thu, 08 Oct 2020 20:12:19: 12000000 INFO @ Thu, 08 Oct 2020 20:12:25: 9000000 INFO @ Thu, 08 Oct 2020 20:12:27: 16000000 INFO @ Thu, 08 Oct 2020 20:12:27: 13000000 INFO @ Thu, 08 Oct 2020 20:12:34: 10000000 INFO @ Thu, 08 Oct 2020 20:12:35: 17000000 INFO @ Thu, 08 Oct 2020 20:12:35: 14000000 INFO @ Thu, 08 Oct 2020 20:12:41: 11000000 INFO @ Thu, 08 Oct 2020 20:12:43: 18000000 INFO @ Thu, 08 Oct 2020 20:12:43: 15000000 INFO @ Thu, 08 Oct 2020 20:12:49: 12000000 INFO @ Thu, 08 Oct 2020 20:12:51: 16000000 INFO @ Thu, 08 Oct 2020 20:12:52: 19000000 INFO @ Thu, 08 Oct 2020 20:12:57: 13000000 INFO @ Thu, 08 Oct 2020 20:12:59: 17000000 INFO @ Thu, 08 Oct 2020 20:13:00: 20000000 INFO @ Thu, 08 Oct 2020 20:13:06: 14000000 INFO @ Thu, 08 Oct 2020 20:13:07: 18000000 INFO @ Thu, 08 Oct 2020 20:13:08: 21000000 INFO @ Thu, 08 Oct 2020 20:13:14: 15000000 INFO @ Thu, 08 Oct 2020 20:13:16: 19000000 INFO @ Thu, 08 Oct 2020 20:13:17: 22000000 INFO @ Thu, 08 Oct 2020 20:13:22: 16000000 INFO @ Thu, 08 Oct 2020 20:13:24: 20000000 INFO @ Thu, 08 Oct 2020 20:13:25: 23000000 INFO @ Thu, 08 Oct 2020 20:13:30: 17000000 INFO @ Thu, 08 Oct 2020 20:13:32: 21000000 INFO @ Thu, 08 Oct 2020 20:13:33: 24000000 INFO @ Thu, 08 Oct 2020 20:13:38: 18000000 INFO @ Thu, 08 Oct 2020 20:13:40: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:13:41: 25000000 INFO @ Thu, 08 Oct 2020 20:13:46: 19000000 INFO @ Thu, 08 Oct 2020 20:13:48: 23000000 INFO @ Thu, 08 Oct 2020 20:13:50: 26000000 INFO @ Thu, 08 Oct 2020 20:13:54: 20000000 INFO @ Thu, 08 Oct 2020 20:13:56: 24000000 INFO @ Thu, 08 Oct 2020 20:13:58: 27000000 INFO @ Thu, 08 Oct 2020 20:14:02: 21000000 INFO @ Thu, 08 Oct 2020 20:14:04: 25000000 INFO @ Thu, 08 Oct 2020 20:14:06: 28000000 INFO @ Thu, 08 Oct 2020 20:14:10: 22000000 INFO @ Thu, 08 Oct 2020 20:14:12: 26000000 INFO @ Thu, 08 Oct 2020 20:14:14: 29000000 INFO @ Thu, 08 Oct 2020 20:14:18: 23000000 INFO @ Thu, 08 Oct 2020 20:14:19: 27000000 INFO @ Thu, 08 Oct 2020 20:14:22: 30000000 INFO @ Thu, 08 Oct 2020 20:14:25: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:14:25: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:14:25: #1 total tags in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:14:25: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:14:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:14:26: #1 tags after filtering in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:14:26: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:14:26: #1 finished! INFO @ Thu, 08 Oct 2020 20:14:26: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:14:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:14:26: 24000000 INFO @ Thu, 08 Oct 2020 20:14:27: 28000000 INFO @ Thu, 08 Oct 2020 20:14:28: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:14:28: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:14:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:14:33: 25000000 INFO @ Thu, 08 Oct 2020 20:14:35: 29000000 INFO @ Thu, 08 Oct 2020 20:14:41: 26000000 INFO @ Thu, 08 Oct 2020 20:14:42: 30000000 INFO @ Thu, 08 Oct 2020 20:14:45: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:14:45: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:14:45: #1 total tags in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:14:45: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:14:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:14:46: #1 tags after filtering in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:14:46: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:14:46: #1 finished! INFO @ Thu, 08 Oct 2020 20:14:46: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:14:46: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:14:48: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:14:48: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:14:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:14:48: 27000000 INFO @ Thu, 08 Oct 2020 20:14:56: 28000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:15:04: 29000000 INFO @ Thu, 08 Oct 2020 20:15:12: 30000000 INFO @ Thu, 08 Oct 2020 20:15:15: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:15:15: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:15:15: #1 total tags in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:15:15: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:15:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:15:15: #1 tags after filtering in treatment: 30379513 INFO @ Thu, 08 Oct 2020 20:15:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:15:15: #1 finished! INFO @ Thu, 08 Oct 2020 20:15:15: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:15:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:15:17: #2 number of paired peaks: 98 WARNING @ Thu, 08 Oct 2020 20:15:17: Too few paired peaks (98) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:15:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX9120610/SRX9120610.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling