Job ID = 14160497 SRX = SRX8331146 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 15578265 spots for SRR11778459/SRR11778459.sra Written 15578265 spots for SRR11778459/SRR11778459.sra fastq に変換しました。 bowtie でマッピング中... Your job 14160637 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:41 15578265 reads; of these: 15578265 (100.00%) were unpaired; of these: 3494623 (22.43%) aligned 0 times 8313689 (53.37%) aligned exactly 1 time 3769953 (24.20%) aligned >1 times 77.57% overall alignment rate Time searching: 00:05:41 Overall time: 00:05:41 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 11555599 / 12083642 = 0.9563 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:00:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:00:41: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:00:41: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:00:45: #1 tag size is determined as 63 bps INFO @ Thu, 09 Dec 2021 03:00:45: #1 tag size = 63 INFO @ Thu, 09 Dec 2021 03:00:45: #1 total tags in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:00:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:00:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:00:45: #1 tags after filtering in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:00:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:00:45: #1 finished! INFO @ Thu, 09 Dec 2021 03:00:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:00:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:00:45: #2 number of paired peaks: 2259 INFO @ Thu, 09 Dec 2021 03:00:45: start model_add_line... INFO @ Thu, 09 Dec 2021 03:00:45: start X-correlation... INFO @ Thu, 09 Dec 2021 03:00:45: end of X-cor INFO @ Thu, 09 Dec 2021 03:00:45: #2 finished! INFO @ Thu, 09 Dec 2021 03:00:45: #2 predicted fragment length is 70 bps INFO @ Thu, 09 Dec 2021 03:00:45: #2 alternative fragment length(s) may be 70 bps INFO @ Thu, 09 Dec 2021 03:00:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05_model.r WARNING @ Thu, 09 Dec 2021 03:00:45: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:00:45: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Thu, 09 Dec 2021 03:00:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:00:45: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:00:45: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:00:46: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:00:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05_peaks.xls INFO @ Thu, 09 Dec 2021 03:00:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:00:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.05_summits.bed INFO @ Thu, 09 Dec 2021 03:00:47: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (1294 records, 4 fields): 3 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:01:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:01:11: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:01:11: #1 read treatment tags... INFO @ Thu, 09 Dec 2021 03:01:15: #1 tag size is determined as 63 bps INFO @ Thu, 09 Dec 2021 03:01:15: #1 tag size = 63 INFO @ Thu, 09 Dec 2021 03:01:15: #1 total tags in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:01:15: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:01:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:01:15: #1 tags after filtering in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:01:15: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:01:15: #1 finished! INFO @ Thu, 09 Dec 2021 03:01:15: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:01:15: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:01:15: #2 number of paired peaks: 2259 INFO @ Thu, 09 Dec 2021 03:01:15: start model_add_line... INFO @ Thu, 09 Dec 2021 03:01:15: start X-correlation... INFO @ Thu, 09 Dec 2021 03:01:15: end of X-cor INFO @ Thu, 09 Dec 2021 03:01:15: #2 finished! INFO @ Thu, 09 Dec 2021 03:01:15: #2 predicted fragment length is 70 bps INFO @ Thu, 09 Dec 2021 03:01:15: #2 alternative fragment length(s) may be 70 bps INFO @ Thu, 09 Dec 2021 03:01:15: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10_model.r WARNING @ Thu, 09 Dec 2021 03:01:15: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:01:15: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Thu, 09 Dec 2021 03:01:15: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:01:15: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:01:15: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:01:16: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:01:17: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10_peaks.xls INFO @ Thu, 09 Dec 2021 03:01:17: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:01:17: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.10_summits.bed INFO @ Thu, 09 Dec 2021 03:01:17: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (731 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 09 Dec 2021 03:01:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 09 Dec 2021 03:01:41: #1 read tag files... INFO @ Thu, 09 Dec 2021 03:01:41: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Thu, 09 Dec 2021 03:01:45: #1 tag size is determined as 63 bps INFO @ Thu, 09 Dec 2021 03:01:45: #1 tag size = 63 INFO @ Thu, 09 Dec 2021 03:01:45: #1 total tags in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:01:45: #1 user defined the maximum tags... INFO @ Thu, 09 Dec 2021 03:01:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 09 Dec 2021 03:01:45: #1 tags after filtering in treatment: 528043 INFO @ Thu, 09 Dec 2021 03:01:45: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 09 Dec 2021 03:01:45: #1 finished! INFO @ Thu, 09 Dec 2021 03:01:45: #2 Build Peak Model... INFO @ Thu, 09 Dec 2021 03:01:45: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 09 Dec 2021 03:01:45: #2 number of paired peaks: 2259 INFO @ Thu, 09 Dec 2021 03:01:45: start model_add_line... INFO @ Thu, 09 Dec 2021 03:01:45: start X-correlation... INFO @ Thu, 09 Dec 2021 03:01:45: end of X-cor INFO @ Thu, 09 Dec 2021 03:01:45: #2 finished! INFO @ Thu, 09 Dec 2021 03:01:45: #2 predicted fragment length is 70 bps INFO @ Thu, 09 Dec 2021 03:01:45: #2 alternative fragment length(s) may be 70 bps INFO @ Thu, 09 Dec 2021 03:01:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20_model.r WARNING @ Thu, 09 Dec 2021 03:01:45: #2 Since the d (70) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 09 Dec 2021 03:01:45: #2 You may need to consider one of the other alternative d(s): 70 WARNING @ Thu, 09 Dec 2021 03:01:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 09 Dec 2021 03:01:45: #3 Call peaks... INFO @ Thu, 09 Dec 2021 03:01:45: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 09 Dec 2021 03:01:46: #3 Call peaks for each chromosome... INFO @ Thu, 09 Dec 2021 03:01:47: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20_peaks.xls INFO @ Thu, 09 Dec 2021 03:01:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20_peaks.narrowPeak INFO @ Thu, 09 Dec 2021 03:01:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX8331146/SRX8331146.20_summits.bed INFO @ Thu, 09 Dec 2021 03:01:47: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (349 records, 4 fields): 2 millis CompletedMACS2peakCalling