Job ID = 5720343 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 32,524,259 reads read : 32,524,259 reads written : 32,524,259 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:52 32524259 reads; of these: 32524259 (100.00%) were unpaired; of these: 522117 (1.61%) aligned 0 times 28796654 (88.54%) aligned exactly 1 time 3205488 (9.86%) aligned >1 times 98.39% overall alignment rate Time searching: 00:09:52 Overall time: 00:09:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6496547 / 32002142 = 0.2030 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:23:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:23:30: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:23:30: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:23:36: 1000000 INFO @ Thu, 16 Apr 2020 00:23:42: 2000000 INFO @ Thu, 16 Apr 2020 00:23:48: 3000000 INFO @ Thu, 16 Apr 2020 00:23:54: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:24:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:24:00: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:24:00: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:24:00: 5000000 INFO @ Thu, 16 Apr 2020 00:24:07: 1000000 INFO @ Thu, 16 Apr 2020 00:24:07: 6000000 INFO @ Thu, 16 Apr 2020 00:24:14: 2000000 INFO @ Thu, 16 Apr 2020 00:24:14: 7000000 INFO @ Thu, 16 Apr 2020 00:24:21: 3000000 INFO @ Thu, 16 Apr 2020 00:24:21: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:24:27: 4000000 INFO @ Thu, 16 Apr 2020 00:24:28: 9000000 INFO @ Thu, 16 Apr 2020 00:24:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:24:30: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:24:30: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:24:34: 5000000 INFO @ Thu, 16 Apr 2020 00:24:35: 10000000 INFO @ Thu, 16 Apr 2020 00:24:37: 1000000 INFO @ Thu, 16 Apr 2020 00:24:41: 6000000 INFO @ Thu, 16 Apr 2020 00:24:42: 11000000 INFO @ Thu, 16 Apr 2020 00:24:44: 2000000 INFO @ Thu, 16 Apr 2020 00:24:48: 7000000 INFO @ Thu, 16 Apr 2020 00:24:49: 12000000 INFO @ Thu, 16 Apr 2020 00:24:52: 3000000 INFO @ Thu, 16 Apr 2020 00:24:55: 8000000 INFO @ Thu, 16 Apr 2020 00:24:57: 13000000 INFO @ Thu, 16 Apr 2020 00:24:59: 4000000 INFO @ Thu, 16 Apr 2020 00:25:02: 9000000 INFO @ Thu, 16 Apr 2020 00:25:04: 14000000 INFO @ Thu, 16 Apr 2020 00:25:06: 5000000 INFO @ Thu, 16 Apr 2020 00:25:09: 10000000 INFO @ Thu, 16 Apr 2020 00:25:11: 15000000 INFO @ Thu, 16 Apr 2020 00:25:13: 6000000 INFO @ Thu, 16 Apr 2020 00:25:16: 11000000 INFO @ Thu, 16 Apr 2020 00:25:18: 16000000 INFO @ Thu, 16 Apr 2020 00:25:20: 7000000 INFO @ Thu, 16 Apr 2020 00:25:23: 12000000 INFO @ Thu, 16 Apr 2020 00:25:25: 17000000 INFO @ Thu, 16 Apr 2020 00:25:27: 8000000 INFO @ Thu, 16 Apr 2020 00:25:30: 13000000 INFO @ Thu, 16 Apr 2020 00:25:32: 18000000 INFO @ Thu, 16 Apr 2020 00:25:34: 9000000 INFO @ Thu, 16 Apr 2020 00:25:37: 14000000 INFO @ Thu, 16 Apr 2020 00:25:39: 19000000 INFO @ Thu, 16 Apr 2020 00:25:42: 10000000 INFO @ Thu, 16 Apr 2020 00:25:44: 15000000 INFO @ Thu, 16 Apr 2020 00:25:46: 20000000 INFO @ Thu, 16 Apr 2020 00:25:49: 11000000 INFO @ Thu, 16 Apr 2020 00:25:50: 16000000 INFO @ Thu, 16 Apr 2020 00:25:53: 21000000 INFO @ Thu, 16 Apr 2020 00:25:56: 12000000 INFO @ Thu, 16 Apr 2020 00:25:57: 17000000 INFO @ Thu, 16 Apr 2020 00:26:00: 22000000 INFO @ Thu, 16 Apr 2020 00:26:03: 13000000 INFO @ Thu, 16 Apr 2020 00:26:04: 18000000 INFO @ Thu, 16 Apr 2020 00:26:07: 23000000 INFO @ Thu, 16 Apr 2020 00:26:10: 14000000 INFO @ Thu, 16 Apr 2020 00:26:11: 19000000 INFO @ Thu, 16 Apr 2020 00:26:14: 24000000 INFO @ Thu, 16 Apr 2020 00:26:17: 15000000 INFO @ Thu, 16 Apr 2020 00:26:18: 20000000 INFO @ Thu, 16 Apr 2020 00:26:21: 25000000 INFO @ Thu, 16 Apr 2020 00:26:24: 16000000 INFO @ Thu, 16 Apr 2020 00:26:25: 21000000 INFO @ Thu, 16 Apr 2020 00:26:25: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:26:25: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:26:25: #1 total tags in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:26:25: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:26:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:26:25: #1 tags after filtering in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:26:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:26:25: #1 finished! INFO @ Thu, 16 Apr 2020 00:26:25: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:26:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:26:27: #2 number of paired peaks: 58 WARNING @ Thu, 16 Apr 2020 00:26:27: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:26:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:26:31: 17000000 INFO @ Thu, 16 Apr 2020 00:26:32: 22000000 INFO @ Thu, 16 Apr 2020 00:26:38: 18000000 INFO @ Thu, 16 Apr 2020 00:26:39: 23000000 INFO @ Thu, 16 Apr 2020 00:26:45: 19000000 INFO @ Thu, 16 Apr 2020 00:26:46: 24000000 INFO @ Thu, 16 Apr 2020 00:26:52: 20000000 INFO @ Thu, 16 Apr 2020 00:26:52: 25000000 INFO @ Thu, 16 Apr 2020 00:26:57: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:26:57: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:26:57: #1 total tags in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:26:57: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:26:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:26:57: #1 tags after filtering in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:26:57: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:26:57: #1 finished! INFO @ Thu, 16 Apr 2020 00:26:57: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:26:57: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:26:59: 21000000 INFO @ Thu, 16 Apr 2020 00:26:59: #2 number of paired peaks: 58 WARNING @ Thu, 16 Apr 2020 00:26:59: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:26:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:27:05: 22000000 INFO @ Thu, 16 Apr 2020 00:27:12: 23000000 INFO @ Thu, 16 Apr 2020 00:27:19: 24000000 INFO @ Thu, 16 Apr 2020 00:27:25: 25000000 INFO @ Thu, 16 Apr 2020 00:27:29: #1 tag size is determined as 76 bps INFO @ Thu, 16 Apr 2020 00:27:29: #1 tag size = 76 INFO @ Thu, 16 Apr 2020 00:27:29: #1 total tags in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:27:29: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:27:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:27:29: #1 tags after filtering in treatment: 25505595 INFO @ Thu, 16 Apr 2020 00:27:29: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 16 Apr 2020 00:27:29: #1 finished! INFO @ Thu, 16 Apr 2020 00:27:29: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:27:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:27:31: #2 number of paired peaks: 58 WARNING @ Thu, 16 Apr 2020 00:27:31: Too few paired peaks (58) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 16 Apr 2020 00:27:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971805/SRX7971805.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。