Job ID = 5720321 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 27,891,436 reads read : 27,891,436 reads written : 27,891,436 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:22 27891436 reads; of these: 27891436 (100.00%) were unpaired; of these: 506599 (1.82%) aligned 0 times 24669007 (88.45%) aligned exactly 1 time 2715830 (9.74%) aligned >1 times 98.18% overall alignment rate Time searching: 00:08:22 Overall time: 00:08:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 5043171 / 27384837 = 0.1842 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:50:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:50:10: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:50:10: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:50:17: 1000000 INFO @ Wed, 15 Apr 2020 23:50:23: 2000000 INFO @ Wed, 15 Apr 2020 23:50:29: 3000000 INFO @ Wed, 15 Apr 2020 23:50:36: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:50:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:50:40: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:50:40: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:50:42: 5000000 INFO @ Wed, 15 Apr 2020 23:50:47: 1000000 INFO @ Wed, 15 Apr 2020 23:50:49: 6000000 INFO @ Wed, 15 Apr 2020 23:50:54: 2000000 INFO @ Wed, 15 Apr 2020 23:50:55: 7000000 INFO @ Wed, 15 Apr 2020 23:51:00: 3000000 INFO @ Wed, 15 Apr 2020 23:51:02: 8000000 INFO @ Wed, 15 Apr 2020 23:51:07: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 15 Apr 2020 23:51:09: 9000000 INFO @ Wed, 15 Apr 2020 23:51:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 15 Apr 2020 23:51:10: #1 read tag files... INFO @ Wed, 15 Apr 2020 23:51:10: #1 read treatment tags... INFO @ Wed, 15 Apr 2020 23:51:14: 5000000 INFO @ Wed, 15 Apr 2020 23:51:15: 10000000 INFO @ Wed, 15 Apr 2020 23:51:17: 1000000 INFO @ Wed, 15 Apr 2020 23:51:21: 6000000 INFO @ Wed, 15 Apr 2020 23:51:22: 11000000 INFO @ Wed, 15 Apr 2020 23:51:24: 2000000 INFO @ Wed, 15 Apr 2020 23:51:28: 7000000 INFO @ Wed, 15 Apr 2020 23:51:28: 12000000 INFO @ Wed, 15 Apr 2020 23:51:31: 3000000 INFO @ Wed, 15 Apr 2020 23:51:34: 8000000 INFO @ Wed, 15 Apr 2020 23:51:35: 13000000 INFO @ Wed, 15 Apr 2020 23:51:38: 4000000 INFO @ Wed, 15 Apr 2020 23:51:41: 9000000 INFO @ Wed, 15 Apr 2020 23:51:42: 14000000 INFO @ Wed, 15 Apr 2020 23:51:45: 5000000 INFO @ Wed, 15 Apr 2020 23:51:47: 10000000 INFO @ Wed, 15 Apr 2020 23:51:48: 15000000 INFO @ Wed, 15 Apr 2020 23:51:51: 6000000 INFO @ Wed, 15 Apr 2020 23:51:54: 11000000 INFO @ Wed, 15 Apr 2020 23:51:55: 16000000 INFO @ Wed, 15 Apr 2020 23:51:58: 7000000 INFO @ Wed, 15 Apr 2020 23:52:00: 12000000 INFO @ Wed, 15 Apr 2020 23:52:01: 17000000 INFO @ Wed, 15 Apr 2020 23:52:05: 8000000 INFO @ Wed, 15 Apr 2020 23:52:07: 13000000 INFO @ Wed, 15 Apr 2020 23:52:08: 18000000 INFO @ Wed, 15 Apr 2020 23:52:11: 9000000 INFO @ Wed, 15 Apr 2020 23:52:14: 14000000 INFO @ Wed, 15 Apr 2020 23:52:15: 19000000 INFO @ Wed, 15 Apr 2020 23:52:18: 10000000 INFO @ Wed, 15 Apr 2020 23:52:21: 15000000 INFO @ Wed, 15 Apr 2020 23:52:22: 20000000 INFO @ Wed, 15 Apr 2020 23:52:24: 11000000 INFO @ Wed, 15 Apr 2020 23:52:27: 16000000 INFO @ Wed, 15 Apr 2020 23:52:28: 21000000 INFO @ Wed, 15 Apr 2020 23:52:31: 12000000 INFO @ Wed, 15 Apr 2020 23:52:34: 17000000 INFO @ Wed, 15 Apr 2020 23:52:35: 22000000 INFO @ Wed, 15 Apr 2020 23:52:38: #1 tag size is determined as 76 bps INFO @ Wed, 15 Apr 2020 23:52:38: #1 tag size = 76 INFO @ Wed, 15 Apr 2020 23:52:38: #1 total tags in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:52:38: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:52:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:52:38: 13000000 INFO @ Wed, 15 Apr 2020 23:52:38: #1 tags after filtering in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:52:38: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 23:52:38: #1 finished! INFO @ Wed, 15 Apr 2020 23:52:38: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:52:38: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:52:39: #2 number of paired peaks: 54 WARNING @ Wed, 15 Apr 2020 23:52:39: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 23:52:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:52:41: 18000000 INFO @ Wed, 15 Apr 2020 23:52:45: 14000000 INFO @ Wed, 15 Apr 2020 23:52:48: 19000000 INFO @ Wed, 15 Apr 2020 23:52:52: 15000000 INFO @ Wed, 15 Apr 2020 23:52:55: 20000000 INFO @ Wed, 15 Apr 2020 23:52:58: 16000000 INFO @ Wed, 15 Apr 2020 23:53:01: 21000000 INFO @ Wed, 15 Apr 2020 23:53:05: 17000000 INFO @ Wed, 15 Apr 2020 23:53:08: 22000000 INFO @ Wed, 15 Apr 2020 23:53:10: #1 tag size is determined as 76 bps INFO @ Wed, 15 Apr 2020 23:53:10: #1 tag size = 76 INFO @ Wed, 15 Apr 2020 23:53:10: #1 total tags in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:53:10: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:53:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:53:11: #1 tags after filtering in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:53:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 23:53:11: #1 finished! INFO @ Wed, 15 Apr 2020 23:53:11: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:53:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:53:12: 18000000 INFO @ Wed, 15 Apr 2020 23:53:12: #2 number of paired peaks: 54 WARNING @ Wed, 15 Apr 2020 23:53:12: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 23:53:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 15 Apr 2020 23:53:18: 19000000 INFO @ Wed, 15 Apr 2020 23:53:25: 20000000 INFO @ Wed, 15 Apr 2020 23:53:31: 21000000 INFO @ Wed, 15 Apr 2020 23:53:38: 22000000 INFO @ Wed, 15 Apr 2020 23:53:40: #1 tag size is determined as 76 bps INFO @ Wed, 15 Apr 2020 23:53:40: #1 tag size = 76 INFO @ Wed, 15 Apr 2020 23:53:40: #1 total tags in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:53:40: #1 user defined the maximum tags... INFO @ Wed, 15 Apr 2020 23:53:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 15 Apr 2020 23:53:41: #1 tags after filtering in treatment: 22341666 INFO @ Wed, 15 Apr 2020 23:53:41: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 15 Apr 2020 23:53:41: #1 finished! INFO @ Wed, 15 Apr 2020 23:53:41: #2 Build Peak Model... INFO @ Wed, 15 Apr 2020 23:53:41: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 15 Apr 2020 23:53:42: #2 number of paired peaks: 54 WARNING @ Wed, 15 Apr 2020 23:53:42: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 15 Apr 2020 23:53:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7971792/SRX7971792.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。