Job ID = 10165651 SRX = SRX7879258 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39732741 spots for SRR11272873/SRR11272873.sra Written 39732741 spots for SRR11272873/SRR11272873.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165937 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:15 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 646226 (1.63%) aligned 0 times 33452610 (84.19%) aligned exactly 1 time 5633905 (14.18%) aligned >1 times 98.37% overall alignment rate Time searching: 00:09:15 Overall time: 00:09:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6880942 / 39086515 = 0.1760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:20: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:20: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:27: 1000000 INFO @ Thu, 08 Oct 2020 19:51:34: 2000000 INFO @ Thu, 08 Oct 2020 19:51:40: 3000000 INFO @ Thu, 08 Oct 2020 19:51:47: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:50: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:50: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:54: 5000000 INFO @ Thu, 08 Oct 2020 19:51:57: 1000000 INFO @ Thu, 08 Oct 2020 19:52:01: 6000000 INFO @ Thu, 08 Oct 2020 19:52:04: 2000000 INFO @ Thu, 08 Oct 2020 19:52:09: 7000000 INFO @ Thu, 08 Oct 2020 19:52:10: 3000000 INFO @ Thu, 08 Oct 2020 19:52:16: 8000000 INFO @ Thu, 08 Oct 2020 19:52:17: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:20: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:20: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:24: 9000000 INFO @ Thu, 08 Oct 2020 19:52:24: 5000000 INFO @ Thu, 08 Oct 2020 19:52:28: 1000000 INFO @ Thu, 08 Oct 2020 19:52:31: 6000000 INFO @ Thu, 08 Oct 2020 19:52:31: 10000000 INFO @ Thu, 08 Oct 2020 19:52:36: 2000000 INFO @ Thu, 08 Oct 2020 19:52:38: 7000000 INFO @ Thu, 08 Oct 2020 19:52:39: 11000000 INFO @ Thu, 08 Oct 2020 19:52:44: 3000000 INFO @ Thu, 08 Oct 2020 19:52:45: 8000000 INFO @ Thu, 08 Oct 2020 19:52:47: 12000000 INFO @ Thu, 08 Oct 2020 19:52:51: 4000000 INFO @ Thu, 08 Oct 2020 19:52:52: 9000000 INFO @ Thu, 08 Oct 2020 19:52:55: 13000000 INFO @ Thu, 08 Oct 2020 19:52:59: 10000000 INFO @ Thu, 08 Oct 2020 19:52:59: 5000000 INFO @ Thu, 08 Oct 2020 19:53:02: 14000000 INFO @ Thu, 08 Oct 2020 19:53:06: 11000000 INFO @ Thu, 08 Oct 2020 19:53:07: 6000000 INFO @ Thu, 08 Oct 2020 19:53:10: 15000000 INFO @ Thu, 08 Oct 2020 19:53:13: 12000000 INFO @ Thu, 08 Oct 2020 19:53:15: 7000000 INFO @ Thu, 08 Oct 2020 19:53:17: 16000000 INFO @ Thu, 08 Oct 2020 19:53:20: 13000000 INFO @ Thu, 08 Oct 2020 19:53:22: 8000000 INFO @ Thu, 08 Oct 2020 19:53:25: 17000000 INFO @ Thu, 08 Oct 2020 19:53:27: 14000000 INFO @ Thu, 08 Oct 2020 19:53:30: 9000000 INFO @ Thu, 08 Oct 2020 19:53:33: 18000000 INFO @ Thu, 08 Oct 2020 19:53:34: 15000000 INFO @ Thu, 08 Oct 2020 19:53:38: 10000000 INFO @ Thu, 08 Oct 2020 19:53:40: 19000000 INFO @ Thu, 08 Oct 2020 19:53:41: 16000000 INFO @ Thu, 08 Oct 2020 19:53:46: 11000000 INFO @ Thu, 08 Oct 2020 19:53:48: 17000000 INFO @ Thu, 08 Oct 2020 19:53:48: 20000000 INFO @ Thu, 08 Oct 2020 19:53:53: 12000000 INFO @ Thu, 08 Oct 2020 19:53:55: 18000000 INFO @ Thu, 08 Oct 2020 19:53:56: 21000000 INFO @ Thu, 08 Oct 2020 19:54:01: 13000000 INFO @ Thu, 08 Oct 2020 19:54:02: 19000000 INFO @ Thu, 08 Oct 2020 19:54:03: 22000000 INFO @ Thu, 08 Oct 2020 19:54:09: 20000000 INFO @ Thu, 08 Oct 2020 19:54:09: 14000000 INFO @ Thu, 08 Oct 2020 19:54:11: 23000000 INFO @ Thu, 08 Oct 2020 19:54:16: 21000000 INFO @ Thu, 08 Oct 2020 19:54:17: 15000000 INFO @ Thu, 08 Oct 2020 19:54:19: 24000000 INFO @ Thu, 08 Oct 2020 19:54:23: 22000000 INFO @ Thu, 08 Oct 2020 19:54:24: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:54:27: 25000000 INFO @ Thu, 08 Oct 2020 19:54:30: 23000000 INFO @ Thu, 08 Oct 2020 19:54:32: 17000000 INFO @ Thu, 08 Oct 2020 19:54:35: 26000000 INFO @ Thu, 08 Oct 2020 19:54:38: 24000000 INFO @ Thu, 08 Oct 2020 19:54:40: 18000000 INFO @ Thu, 08 Oct 2020 19:54:43: 27000000 INFO @ Thu, 08 Oct 2020 19:54:45: 25000000 INFO @ Thu, 08 Oct 2020 19:54:48: 19000000 INFO @ Thu, 08 Oct 2020 19:54:51: 28000000 INFO @ Thu, 08 Oct 2020 19:54:52: 26000000 INFO @ Thu, 08 Oct 2020 19:54:55: 20000000 INFO @ Thu, 08 Oct 2020 19:54:59: 29000000 INFO @ Thu, 08 Oct 2020 19:54:59: 27000000 INFO @ Thu, 08 Oct 2020 19:55:03: 21000000 INFO @ Thu, 08 Oct 2020 19:55:07: 28000000 INFO @ Thu, 08 Oct 2020 19:55:07: 30000000 INFO @ Thu, 08 Oct 2020 19:55:11: 22000000 INFO @ Thu, 08 Oct 2020 19:55:14: 29000000 INFO @ Thu, 08 Oct 2020 19:55:15: 31000000 INFO @ Thu, 08 Oct 2020 19:55:19: 23000000 INFO @ Thu, 08 Oct 2020 19:55:21: 30000000 INFO @ Thu, 08 Oct 2020 19:55:23: 32000000 INFO @ Thu, 08 Oct 2020 19:55:24: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:24: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:24: #1 total tags in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:55:24: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:55:25: #1 tags after filtering in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:55:25: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:25: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:25: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:27: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 19:55:27: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:27: 24000000 INFO @ Thu, 08 Oct 2020 19:55:28: 31000000 INFO @ Thu, 08 Oct 2020 19:55:35: 25000000 INFO @ Thu, 08 Oct 2020 19:55:36: 32000000 INFO @ Thu, 08 Oct 2020 19:55:37: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:37: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:37: #1 total tags in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:55:37: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:38: #1 tags after filtering in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:55:38: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:38: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:38: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:38: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:40: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 19:55:40: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:42: 26000000 INFO @ Thu, 08 Oct 2020 19:55:49: 27000000 INFO @ Thu, 08 Oct 2020 19:55:57: 28000000 INFO @ Thu, 08 Oct 2020 19:56:04: 29000000 INFO @ Thu, 08 Oct 2020 19:56:11: 30000000 INFO @ Thu, 08 Oct 2020 19:56:18: 31000000 INFO @ Thu, 08 Oct 2020 19:56:25: 32000000 INFO @ Thu, 08 Oct 2020 19:56:26: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:26: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:26: #1 total tags in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:56:26: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:27: #1 tags after filtering in treatment: 32205573 INFO @ Thu, 08 Oct 2020 19:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:27: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:27: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:29: #2 number of paired peaks: 23 WARNING @ Thu, 08 Oct 2020 19:56:29: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling