Job ID = 14157850 SRX = SRX7879258 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 39732741 spots for SRR11272873/SRR11272873.sra Written 39732741 spots for SRR11272873/SRR11272873.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157992 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:38 39732741 reads; of these: 39732741 (100.00%) were unpaired; of these: 646226 (1.63%) aligned 0 times 33452610 (84.19%) aligned exactly 1 time 5633905 (14.18%) aligned >1 times 98.37% overall alignment rate Time searching: 00:08:38 Overall time: 00:08:38 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 6880942 / 39086515 = 0.1760 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:43:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:43:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:43:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:43:40: 1000000 INFO @ Wed, 08 Dec 2021 11:43:47: 2000000 INFO @ Wed, 08 Dec 2021 11:43:54: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:44:01: 4000000 INFO @ Wed, 08 Dec 2021 11:44:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:44:02: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:44:02: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:44:09: 5000000 INFO @ Wed, 08 Dec 2021 11:44:10: 1000000 INFO @ Wed, 08 Dec 2021 11:44:17: 6000000 INFO @ Wed, 08 Dec 2021 11:44:17: 2000000 INFO @ Wed, 08 Dec 2021 11:44:25: 3000000 INFO @ Wed, 08 Dec 2021 11:44:25: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:44:32: 4000000 INFO @ Wed, 08 Dec 2021 11:44:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:44:32: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:44:32: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:44:33: 8000000 INFO @ Wed, 08 Dec 2021 11:44:39: 5000000 INFO @ Wed, 08 Dec 2021 11:44:41: 9000000 INFO @ Wed, 08 Dec 2021 11:44:41: 1000000 INFO @ Wed, 08 Dec 2021 11:44:47: 6000000 INFO @ Wed, 08 Dec 2021 11:44:49: 10000000 INFO @ Wed, 08 Dec 2021 11:44:49: 2000000 INFO @ Wed, 08 Dec 2021 11:44:55: 7000000 INFO @ Wed, 08 Dec 2021 11:44:57: 11000000 INFO @ Wed, 08 Dec 2021 11:44:57: 3000000 INFO @ Wed, 08 Dec 2021 11:45:02: 8000000 INFO @ Wed, 08 Dec 2021 11:45:05: 4000000 INFO @ Wed, 08 Dec 2021 11:45:05: 12000000 INFO @ Wed, 08 Dec 2021 11:45:09: 9000000 INFO @ Wed, 08 Dec 2021 11:45:13: 13000000 INFO @ Wed, 08 Dec 2021 11:45:13: 5000000 INFO @ Wed, 08 Dec 2021 11:45:17: 10000000 INFO @ Wed, 08 Dec 2021 11:45:21: 14000000 INFO @ Wed, 08 Dec 2021 11:45:22: 6000000 INFO @ Wed, 08 Dec 2021 11:45:24: 11000000 INFO @ Wed, 08 Dec 2021 11:45:30: 7000000 INFO @ Wed, 08 Dec 2021 11:45:30: 15000000 INFO @ Wed, 08 Dec 2021 11:45:32: 12000000 INFO @ Wed, 08 Dec 2021 11:45:38: 8000000 INFO @ Wed, 08 Dec 2021 11:45:38: 16000000 INFO @ Wed, 08 Dec 2021 11:45:39: 13000000 INFO @ Wed, 08 Dec 2021 11:45:46: 9000000 INFO @ Wed, 08 Dec 2021 11:45:46: 17000000 INFO @ Wed, 08 Dec 2021 11:45:47: 14000000 INFO @ Wed, 08 Dec 2021 11:45:54: 10000000 INFO @ Wed, 08 Dec 2021 11:45:54: 15000000 INFO @ Wed, 08 Dec 2021 11:45:55: 18000000 INFO @ Wed, 08 Dec 2021 11:46:02: 11000000 INFO @ Wed, 08 Dec 2021 11:46:02: 16000000 INFO @ Wed, 08 Dec 2021 11:46:03: 19000000 INFO @ Wed, 08 Dec 2021 11:46:10: 17000000 INFO @ Wed, 08 Dec 2021 11:46:10: 12000000 INFO @ Wed, 08 Dec 2021 11:46:11: 20000000 INFO @ Wed, 08 Dec 2021 11:46:17: 18000000 INFO @ Wed, 08 Dec 2021 11:46:18: 13000000 INFO @ Wed, 08 Dec 2021 11:46:20: 21000000 INFO @ Wed, 08 Dec 2021 11:46:25: 19000000 INFO @ Wed, 08 Dec 2021 11:46:26: 14000000 INFO @ Wed, 08 Dec 2021 11:46:28: 22000000 INFO @ Wed, 08 Dec 2021 11:46:32: 20000000 INFO @ Wed, 08 Dec 2021 11:46:35: 15000000 INFO @ Wed, 08 Dec 2021 11:46:36: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:46:40: 21000000 INFO @ Wed, 08 Dec 2021 11:46:43: 16000000 INFO @ Wed, 08 Dec 2021 11:46:45: 24000000 INFO @ Wed, 08 Dec 2021 11:46:48: 22000000 INFO @ Wed, 08 Dec 2021 11:46:51: 17000000 INFO @ Wed, 08 Dec 2021 11:46:53: 25000000 INFO @ Wed, 08 Dec 2021 11:46:55: 23000000 INFO @ Wed, 08 Dec 2021 11:47:00: 18000000 INFO @ Wed, 08 Dec 2021 11:47:02: 26000000 INFO @ Wed, 08 Dec 2021 11:47:03: 24000000 INFO @ Wed, 08 Dec 2021 11:47:08: 19000000 INFO @ Wed, 08 Dec 2021 11:47:10: 27000000 INFO @ Wed, 08 Dec 2021 11:47:10: 25000000 INFO @ Wed, 08 Dec 2021 11:47:17: 20000000 INFO @ Wed, 08 Dec 2021 11:47:18: 26000000 INFO @ Wed, 08 Dec 2021 11:47:19: 28000000 INFO @ Wed, 08 Dec 2021 11:47:25: 21000000 INFO @ Wed, 08 Dec 2021 11:47:26: 27000000 INFO @ Wed, 08 Dec 2021 11:47:27: 29000000 INFO @ Wed, 08 Dec 2021 11:47:33: 28000000 INFO @ Wed, 08 Dec 2021 11:47:34: 22000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:47:35: 30000000 INFO @ Wed, 08 Dec 2021 11:47:41: 29000000 INFO @ Wed, 08 Dec 2021 11:47:42: 23000000 INFO @ Wed, 08 Dec 2021 11:47:44: 31000000 INFO @ Wed, 08 Dec 2021 11:47:49: 30000000 INFO @ Wed, 08 Dec 2021 11:47:50: 24000000 INFO @ Wed, 08 Dec 2021 11:47:52: 32000000 INFO @ Wed, 08 Dec 2021 11:47:54: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:47:54: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:47:54: #1 total tags in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:47:54: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:47:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:47:55: #1 tags after filtering in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:47:55: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:47:55: #1 finished! INFO @ Wed, 08 Dec 2021 11:47:55: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:47:55: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:47:56: 31000000 INFO @ Wed, 08 Dec 2021 11:47:56: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 11:47:56: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:47:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:47:59: 25000000 INFO @ Wed, 08 Dec 2021 11:48:04: 32000000 INFO @ Wed, 08 Dec 2021 11:48:05: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:48:05: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:48:05: #1 total tags in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:48:05: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:48:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:48:06: #1 tags after filtering in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:48:06: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:48:06: #1 finished! INFO @ Wed, 08 Dec 2021 11:48:06: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:48:06: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:48:07: 26000000 INFO @ Wed, 08 Dec 2021 11:48:08: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 11:48:08: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:48:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:48:14: 27000000 INFO @ Wed, 08 Dec 2021 11:48:22: 28000000 INFO @ Wed, 08 Dec 2021 11:48:29: 29000000 INFO @ Wed, 08 Dec 2021 11:48:37: 30000000 INFO @ Wed, 08 Dec 2021 11:48:44: 31000000 INFO @ Wed, 08 Dec 2021 11:48:51: 32000000 INFO @ Wed, 08 Dec 2021 11:48:53: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:48:53: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:48:53: #1 total tags in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:48:53: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:48:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:48:53: #1 tags after filtering in treatment: 32205573 INFO @ Wed, 08 Dec 2021 11:48:53: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:48:53: #1 finished! INFO @ Wed, 08 Dec 2021 11:48:53: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:48:53: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:48:55: #2 number of paired peaks: 23 WARNING @ Wed, 08 Dec 2021 11:48:55: Too few paired peaks (23) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:48:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879258/SRX7879258.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling