Job ID = 14157847 SRX = SRX7879255 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42913454 spots for SRR11272870/SRR11272870.sra Written 42913454 spots for SRR11272870/SRR11272870.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157980 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:22 42913454 reads; of these: 42913454 (100.00%) were unpaired; of these: 530202 (1.24%) aligned 0 times 35925131 (83.72%) aligned exactly 1 time 6458121 (15.05%) aligned >1 times 98.76% overall alignment rate Time searching: 00:09:22 Overall time: 00:09:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7521924 / 42383252 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:38:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:38:18: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:38:18: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:38:23: 1000000 INFO @ Wed, 08 Dec 2021 11:38:29: 2000000 INFO @ Wed, 08 Dec 2021 11:38:34: 3000000 INFO @ Wed, 08 Dec 2021 11:38:40: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:38:46: 5000000 INFO @ Wed, 08 Dec 2021 11:38:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:38:47: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:38:47: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:38:52: 6000000 INFO @ Wed, 08 Dec 2021 11:38:53: 1000000 INFO @ Wed, 08 Dec 2021 11:38:57: 7000000 INFO @ Wed, 08 Dec 2021 11:38:59: 2000000 INFO @ Wed, 08 Dec 2021 11:39:03: 8000000 INFO @ Wed, 08 Dec 2021 11:39:04: 3000000 INFO @ Wed, 08 Dec 2021 11:39:09: 9000000 INFO @ Wed, 08 Dec 2021 11:39:10: 4000000 INFO @ Wed, 08 Dec 2021 11:39:15: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:39:16: 5000000 INFO @ Wed, 08 Dec 2021 11:39:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:39:17: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:39:17: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:39:21: 11000000 INFO @ Wed, 08 Dec 2021 11:39:22: 6000000 INFO @ Wed, 08 Dec 2021 11:39:23: 1000000 INFO @ Wed, 08 Dec 2021 11:39:27: 12000000 INFO @ Wed, 08 Dec 2021 11:39:28: 7000000 INFO @ Wed, 08 Dec 2021 11:39:28: 2000000 INFO @ Wed, 08 Dec 2021 11:39:33: 13000000 INFO @ Wed, 08 Dec 2021 11:39:33: 3000000 INFO @ Wed, 08 Dec 2021 11:39:33: 8000000 INFO @ Wed, 08 Dec 2021 11:39:38: 4000000 INFO @ Wed, 08 Dec 2021 11:39:39: 14000000 INFO @ Wed, 08 Dec 2021 11:39:39: 9000000 INFO @ Wed, 08 Dec 2021 11:39:44: 5000000 INFO @ Wed, 08 Dec 2021 11:39:45: 15000000 INFO @ Wed, 08 Dec 2021 11:39:45: 10000000 INFO @ Wed, 08 Dec 2021 11:39:49: 6000000 INFO @ Wed, 08 Dec 2021 11:39:51: 16000000 INFO @ Wed, 08 Dec 2021 11:39:51: 11000000 INFO @ Wed, 08 Dec 2021 11:39:54: 7000000 INFO @ Wed, 08 Dec 2021 11:39:56: 17000000 INFO @ Wed, 08 Dec 2021 11:39:57: 12000000 INFO @ Wed, 08 Dec 2021 11:39:59: 8000000 INFO @ Wed, 08 Dec 2021 11:40:02: 13000000 INFO @ Wed, 08 Dec 2021 11:40:02: 18000000 INFO @ Wed, 08 Dec 2021 11:40:05: 9000000 INFO @ Wed, 08 Dec 2021 11:40:08: 14000000 INFO @ Wed, 08 Dec 2021 11:40:08: 19000000 INFO @ Wed, 08 Dec 2021 11:40:10: 10000000 INFO @ Wed, 08 Dec 2021 11:40:14: 15000000 INFO @ Wed, 08 Dec 2021 11:40:14: 20000000 INFO @ Wed, 08 Dec 2021 11:40:15: 11000000 INFO @ Wed, 08 Dec 2021 11:40:20: 16000000 INFO @ Wed, 08 Dec 2021 11:40:20: 21000000 INFO @ Wed, 08 Dec 2021 11:40:21: 12000000 INFO @ Wed, 08 Dec 2021 11:40:26: 17000000 INFO @ Wed, 08 Dec 2021 11:40:26: 22000000 INFO @ Wed, 08 Dec 2021 11:40:26: 13000000 INFO @ Wed, 08 Dec 2021 11:40:31: 14000000 INFO @ Wed, 08 Dec 2021 11:40:31: 18000000 INFO @ Wed, 08 Dec 2021 11:40:32: 23000000 INFO @ Wed, 08 Dec 2021 11:40:36: 15000000 INFO @ Wed, 08 Dec 2021 11:40:37: 19000000 INFO @ Wed, 08 Dec 2021 11:40:37: 24000000 INFO @ Wed, 08 Dec 2021 11:40:42: 16000000 INFO @ Wed, 08 Dec 2021 11:40:43: 20000000 INFO @ Wed, 08 Dec 2021 11:40:43: 25000000 INFO @ Wed, 08 Dec 2021 11:40:47: 17000000 INFO @ Wed, 08 Dec 2021 11:40:49: 21000000 INFO @ Wed, 08 Dec 2021 11:40:49: 26000000 INFO @ Wed, 08 Dec 2021 11:40:52: 18000000 INFO @ Wed, 08 Dec 2021 11:40:55: 22000000 INFO @ Wed, 08 Dec 2021 11:40:55: 27000000 INFO @ Wed, 08 Dec 2021 11:40:58: 19000000 INFO @ Wed, 08 Dec 2021 11:41:01: 23000000 INFO @ Wed, 08 Dec 2021 11:41:01: 28000000 INFO @ Wed, 08 Dec 2021 11:41:03: 20000000 INFO @ Wed, 08 Dec 2021 11:41:06: 24000000 INFO @ Wed, 08 Dec 2021 11:41:07: 29000000 INFO @ Wed, 08 Dec 2021 11:41:08: 21000000 INFO @ Wed, 08 Dec 2021 11:41:12: 25000000 INFO @ Wed, 08 Dec 2021 11:41:13: 30000000 INFO @ Wed, 08 Dec 2021 11:41:13: 22000000 INFO @ Wed, 08 Dec 2021 11:41:18: 26000000 INFO @ Wed, 08 Dec 2021 11:41:19: 23000000 INFO @ Wed, 08 Dec 2021 11:41:19: 31000000 INFO @ Wed, 08 Dec 2021 11:41:24: 27000000 INFO @ Wed, 08 Dec 2021 11:41:24: 24000000 INFO @ Wed, 08 Dec 2021 11:41:25: 32000000 INFO @ Wed, 08 Dec 2021 11:41:29: 25000000 INFO @ Wed, 08 Dec 2021 11:41:30: 28000000 INFO @ Wed, 08 Dec 2021 11:41:32: 33000000 INFO @ Wed, 08 Dec 2021 11:41:35: 26000000 INFO @ Wed, 08 Dec 2021 11:41:36: 29000000 INFO @ Wed, 08 Dec 2021 11:41:37: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:41:40: 27000000 INFO @ Wed, 08 Dec 2021 11:41:42: 30000000 INFO @ Wed, 08 Dec 2021 11:41:43: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:41:43: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:41:43: #1 total tags in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:41:43: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:41:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:41:43: #1 tags after filtering in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:41:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:41:43: #1 finished! INFO @ Wed, 08 Dec 2021 11:41:43: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:41:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:41:45: 28000000 INFO @ Wed, 08 Dec 2021 11:41:45: #2 number of paired peaks: 16 WARNING @ Wed, 08 Dec 2021 11:41:45: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:41:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:41:48: 31000000 INFO @ Wed, 08 Dec 2021 11:41:50: 29000000 INFO @ Wed, 08 Dec 2021 11:41:54: 32000000 INFO @ Wed, 08 Dec 2021 11:41:56: 30000000 INFO @ Wed, 08 Dec 2021 11:42:00: 33000000 INFO @ Wed, 08 Dec 2021 11:42:01: 31000000 INFO @ Wed, 08 Dec 2021 11:42:05: 34000000 INFO @ Wed, 08 Dec 2021 11:42:06: 32000000 INFO @ Wed, 08 Dec 2021 11:42:11: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:42:11: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:42:11: #1 total tags in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:42:11: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:42:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:42:11: #1 tags after filtering in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:42:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:42:11: #1 finished! INFO @ Wed, 08 Dec 2021 11:42:11: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:42:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:42:12: 33000000 INFO @ Wed, 08 Dec 2021 11:42:13: #2 number of paired peaks: 16 WARNING @ Wed, 08 Dec 2021 11:42:13: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:42:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:42:17: 34000000 INFO @ Wed, 08 Dec 2021 11:42:21: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:42:21: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:42:21: #1 total tags in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:42:21: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:42:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:42:22: #1 tags after filtering in treatment: 34861328 INFO @ Wed, 08 Dec 2021 11:42:22: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:42:22: #1 finished! INFO @ Wed, 08 Dec 2021 11:42:22: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:42:22: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:42:24: #2 number of paired peaks: 16 WARNING @ Wed, 08 Dec 2021 11:42:24: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:42:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。