Job ID = 10165648 SRX = SRX7879255 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 42913454 spots for SRR11272870/SRR11272870.sra Written 42913454 spots for SRR11272870/SRR11272870.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165938 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:29 42913454 reads; of these: 42913454 (100.00%) were unpaired; of these: 530202 (1.24%) aligned 0 times 35925131 (83.72%) aligned exactly 1 time 6458121 (15.05%) aligned >1 times 98.76% overall alignment rate Time searching: 00:09:29 Overall time: 00:09:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7521924 / 42383252 = 0.1775 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:56: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:56: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:00: 1000000 INFO @ Thu, 08 Oct 2020 19:52:05: 2000000 INFO @ Thu, 08 Oct 2020 19:52:10: 3000000 INFO @ Thu, 08 Oct 2020 19:52:15: 4000000 INFO @ Thu, 08 Oct 2020 19:52:19: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:24: 6000000 INFO @ Thu, 08 Oct 2020 19:52:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:26: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:26: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:29: 7000000 INFO @ Thu, 08 Oct 2020 19:52:31: 1000000 INFO @ Thu, 08 Oct 2020 19:52:34: 8000000 INFO @ Thu, 08 Oct 2020 19:52:36: 2000000 INFO @ Thu, 08 Oct 2020 19:52:39: 9000000 INFO @ Thu, 08 Oct 2020 19:52:41: 3000000 INFO @ Thu, 08 Oct 2020 19:52:44: 10000000 INFO @ Thu, 08 Oct 2020 19:52:46: 4000000 INFO @ Thu, 08 Oct 2020 19:52:49: 11000000 INFO @ Thu, 08 Oct 2020 19:52:51: 5000000 INFO @ Thu, 08 Oct 2020 19:52:54: 12000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:56: 6000000 INFO @ Thu, 08 Oct 2020 19:52:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:56: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:56: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:59: 13000000 INFO @ Thu, 08 Oct 2020 19:53:01: 7000000 INFO @ Thu, 08 Oct 2020 19:53:01: 1000000 INFO @ Thu, 08 Oct 2020 19:53:04: 14000000 INFO @ Thu, 08 Oct 2020 19:53:06: 8000000 INFO @ Thu, 08 Oct 2020 19:53:06: 2000000 INFO @ Thu, 08 Oct 2020 19:53:09: 15000000 INFO @ Thu, 08 Oct 2020 19:53:11: 9000000 INFO @ Thu, 08 Oct 2020 19:53:11: 3000000 INFO @ Thu, 08 Oct 2020 19:53:13: 16000000 INFO @ Thu, 08 Oct 2020 19:53:16: 10000000 INFO @ Thu, 08 Oct 2020 19:53:16: 4000000 INFO @ Thu, 08 Oct 2020 19:53:18: 17000000 INFO @ Thu, 08 Oct 2020 19:53:21: 11000000 INFO @ Thu, 08 Oct 2020 19:53:21: 5000000 INFO @ Thu, 08 Oct 2020 19:53:23: 18000000 INFO @ Thu, 08 Oct 2020 19:53:26: 12000000 INFO @ Thu, 08 Oct 2020 19:53:26: 6000000 INFO @ Thu, 08 Oct 2020 19:53:28: 19000000 INFO @ Thu, 08 Oct 2020 19:53:31: 13000000 INFO @ Thu, 08 Oct 2020 19:53:31: 7000000 INFO @ Thu, 08 Oct 2020 19:53:33: 20000000 INFO @ Thu, 08 Oct 2020 19:53:36: 14000000 INFO @ Thu, 08 Oct 2020 19:53:36: 8000000 INFO @ Thu, 08 Oct 2020 19:53:38: 21000000 INFO @ Thu, 08 Oct 2020 19:53:41: 15000000 INFO @ Thu, 08 Oct 2020 19:53:41: 9000000 INFO @ Thu, 08 Oct 2020 19:53:43: 22000000 INFO @ Thu, 08 Oct 2020 19:53:46: 16000000 INFO @ Thu, 08 Oct 2020 19:53:46: 10000000 INFO @ Thu, 08 Oct 2020 19:53:48: 23000000 INFO @ Thu, 08 Oct 2020 19:53:51: 17000000 INFO @ Thu, 08 Oct 2020 19:53:51: 11000000 INFO @ Thu, 08 Oct 2020 19:53:53: 24000000 INFO @ Thu, 08 Oct 2020 19:53:56: 18000000 INFO @ Thu, 08 Oct 2020 19:53:56: 12000000 INFO @ Thu, 08 Oct 2020 19:53:58: 25000000 INFO @ Thu, 08 Oct 2020 19:54:01: 19000000 INFO @ Thu, 08 Oct 2020 19:54:01: 13000000 INFO @ Thu, 08 Oct 2020 19:54:03: 26000000 INFO @ Thu, 08 Oct 2020 19:54:06: 20000000 INFO @ Thu, 08 Oct 2020 19:54:06: 14000000 INFO @ Thu, 08 Oct 2020 19:54:08: 27000000 INFO @ Thu, 08 Oct 2020 19:54:11: 21000000 INFO @ Thu, 08 Oct 2020 19:54:11: 15000000 INFO @ Thu, 08 Oct 2020 19:54:13: 28000000 INFO @ Thu, 08 Oct 2020 19:54:15: 22000000 INFO @ Thu, 08 Oct 2020 19:54:16: 16000000 INFO @ Thu, 08 Oct 2020 19:54:18: 29000000 INFO @ Thu, 08 Oct 2020 19:54:21: 23000000 INFO @ Thu, 08 Oct 2020 19:54:21: 17000000 INFO @ Thu, 08 Oct 2020 19:54:23: 30000000 INFO @ Thu, 08 Oct 2020 19:54:26: 18000000 INFO @ Thu, 08 Oct 2020 19:54:26: 24000000 INFO @ Thu, 08 Oct 2020 19:54:28: 31000000 INFO @ Thu, 08 Oct 2020 19:54:31: 19000000 INFO @ Thu, 08 Oct 2020 19:54:31: 25000000 INFO @ Thu, 08 Oct 2020 19:54:33: 32000000 INFO @ Thu, 08 Oct 2020 19:54:36: 20000000 INFO @ Thu, 08 Oct 2020 19:54:36: 26000000 INFO @ Thu, 08 Oct 2020 19:54:38: 33000000 INFO @ Thu, 08 Oct 2020 19:54:40: 21000000 INFO @ Thu, 08 Oct 2020 19:54:41: 27000000 INFO @ Thu, 08 Oct 2020 19:54:43: 34000000 INFO @ Thu, 08 Oct 2020 19:54:45: 22000000 INFO @ Thu, 08 Oct 2020 19:54:46: 28000000 INFO @ Thu, 08 Oct 2020 19:54:48: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:54:48: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:54:48: #1 total tags in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:54:48: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:54:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:54:48: #1 tags after filtering in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:54:48: #1 finished! INFO @ Thu, 08 Oct 2020 19:54:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:54:51: 23000000 INFO @ Thu, 08 Oct 2020 19:54:51: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 19:54:51: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:54:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:54:51: 29000000 INFO @ Thu, 08 Oct 2020 19:54:56: 24000000 INFO @ Thu, 08 Oct 2020 19:54:56: 30000000 INFO @ Thu, 08 Oct 2020 19:55:01: 25000000 INFO @ Thu, 08 Oct 2020 19:55:01: 31000000 INFO @ Thu, 08 Oct 2020 19:55:06: 26000000 INFO @ Thu, 08 Oct 2020 19:55:06: 32000000 INFO @ Thu, 08 Oct 2020 19:55:11: 27000000 INFO @ Thu, 08 Oct 2020 19:55:11: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:55:16: 28000000 INFO @ Thu, 08 Oct 2020 19:55:16: 34000000 INFO @ Thu, 08 Oct 2020 19:55:21: 29000000 INFO @ Thu, 08 Oct 2020 19:55:21: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:21: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:21: #1 total tags in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:55:21: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:22: #1 tags after filtering in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:55:22: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:22: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:22: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:22: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:24: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 19:55:24: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:26: 30000000 INFO @ Thu, 08 Oct 2020 19:55:31: 31000000 INFO @ Thu, 08 Oct 2020 19:55:35: 32000000 INFO @ Thu, 08 Oct 2020 19:55:40: 33000000 INFO @ Thu, 08 Oct 2020 19:55:45: 34000000 INFO @ Thu, 08 Oct 2020 19:55:49: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:49: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:49: #1 total tags in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:55:49: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:50: #1 tags after filtering in treatment: 34861328 INFO @ Thu, 08 Oct 2020 19:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:50: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:50: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:52: #2 number of paired peaks: 16 WARNING @ Thu, 08 Oct 2020 19:55:52: Too few paired peaks (16) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879255/SRX7879255.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。