Job ID = 10165644 SRX = SRX7879252 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 60530886 spots for SRR11272867/SRR11272867.sra Written 60530886 spots for SRR11272867/SRR11272867.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165992 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:04 60530886 reads; of these: 60530886 (100.00%) were unpaired; of these: 573321 (0.95%) aligned 0 times 48957167 (80.88%) aligned exactly 1 time 11000398 (18.17%) aligned >1 times 99.05% overall alignment rate Time searching: 00:14:04 Overall time: 00:14:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 13922657 / 59957565 = 0.2322 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:00:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:00:45: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:00:45: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:00:52: 1000000 INFO @ Thu, 08 Oct 2020 20:00:59: 2000000 INFO @ Thu, 08 Oct 2020 20:01:06: 3000000 INFO @ Thu, 08 Oct 2020 20:01:13: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:01:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:01:15: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:01:15: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:01:20: 5000000 INFO @ Thu, 08 Oct 2020 20:01:23: 1000000 INFO @ Thu, 08 Oct 2020 20:01:27: 6000000 INFO @ Thu, 08 Oct 2020 20:01:30: 2000000 INFO @ Thu, 08 Oct 2020 20:01:34: 7000000 INFO @ Thu, 08 Oct 2020 20:01:37: 3000000 INFO @ Thu, 08 Oct 2020 20:01:42: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 20:01:45: 4000000 INFO @ Thu, 08 Oct 2020 20:01:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 20:01:45: #1 read tag files... INFO @ Thu, 08 Oct 2020 20:01:45: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 20:01:49: 9000000 INFO @ Thu, 08 Oct 2020 20:01:52: 5000000 INFO @ Thu, 08 Oct 2020 20:01:53: 1000000 INFO @ Thu, 08 Oct 2020 20:01:57: 10000000 INFO @ Thu, 08 Oct 2020 20:02:00: 6000000 INFO @ Thu, 08 Oct 2020 20:02:00: 2000000 INFO @ Thu, 08 Oct 2020 20:02:04: 11000000 INFO @ Thu, 08 Oct 2020 20:02:07: 7000000 INFO @ Thu, 08 Oct 2020 20:02:07: 3000000 INFO @ Thu, 08 Oct 2020 20:02:12: 12000000 INFO @ Thu, 08 Oct 2020 20:02:15: 8000000 INFO @ Thu, 08 Oct 2020 20:02:15: 4000000 INFO @ Thu, 08 Oct 2020 20:02:20: 13000000 INFO @ Thu, 08 Oct 2020 20:02:22: 5000000 INFO @ Thu, 08 Oct 2020 20:02:22: 9000000 INFO @ Thu, 08 Oct 2020 20:02:28: 14000000 INFO @ Thu, 08 Oct 2020 20:02:29: 6000000 INFO @ Thu, 08 Oct 2020 20:02:30: 10000000 INFO @ Thu, 08 Oct 2020 20:02:35: 15000000 INFO @ Thu, 08 Oct 2020 20:02:37: 7000000 INFO @ Thu, 08 Oct 2020 20:02:38: 11000000 INFO @ Thu, 08 Oct 2020 20:02:43: 16000000 INFO @ Thu, 08 Oct 2020 20:02:44: 8000000 INFO @ Thu, 08 Oct 2020 20:02:45: 12000000 INFO @ Thu, 08 Oct 2020 20:02:51: 17000000 INFO @ Thu, 08 Oct 2020 20:02:51: 9000000 INFO @ Thu, 08 Oct 2020 20:02:53: 13000000 INFO @ Thu, 08 Oct 2020 20:02:58: 10000000 INFO @ Thu, 08 Oct 2020 20:02:59: 18000000 INFO @ Thu, 08 Oct 2020 20:03:01: 14000000 INFO @ Thu, 08 Oct 2020 20:03:06: 11000000 INFO @ Thu, 08 Oct 2020 20:03:07: 19000000 INFO @ Thu, 08 Oct 2020 20:03:08: 15000000 INFO @ Thu, 08 Oct 2020 20:03:13: 12000000 INFO @ Thu, 08 Oct 2020 20:03:15: 20000000 INFO @ Thu, 08 Oct 2020 20:03:16: 16000000 INFO @ Thu, 08 Oct 2020 20:03:20: 13000000 INFO @ Thu, 08 Oct 2020 20:03:23: 17000000 INFO @ Thu, 08 Oct 2020 20:03:23: 21000000 INFO @ Thu, 08 Oct 2020 20:03:27: 14000000 INFO @ Thu, 08 Oct 2020 20:03:31: 18000000 INFO @ Thu, 08 Oct 2020 20:03:31: 22000000 INFO @ Thu, 08 Oct 2020 20:03:34: 15000000 INFO @ Thu, 08 Oct 2020 20:03:38: 19000000 INFO @ Thu, 08 Oct 2020 20:03:39: 23000000 INFO @ Thu, 08 Oct 2020 20:03:42: 16000000 INFO @ Thu, 08 Oct 2020 20:03:46: 20000000 INFO @ Thu, 08 Oct 2020 20:03:47: 24000000 INFO @ Thu, 08 Oct 2020 20:03:49: 17000000 INFO @ Thu, 08 Oct 2020 20:03:53: 21000000 INFO @ Thu, 08 Oct 2020 20:03:55: 25000000 INFO @ Thu, 08 Oct 2020 20:03:56: 18000000 INFO @ Thu, 08 Oct 2020 20:04:01: 22000000 INFO @ Thu, 08 Oct 2020 20:04:02: 26000000 INFO @ Thu, 08 Oct 2020 20:04:03: 19000000 INFO @ Thu, 08 Oct 2020 20:04:08: 23000000 INFO @ Thu, 08 Oct 2020 20:04:10: 20000000 INFO @ Thu, 08 Oct 2020 20:04:10: 27000000 INFO @ Thu, 08 Oct 2020 20:04:15: 24000000 INFO @ Thu, 08 Oct 2020 20:04:17: 21000000 INFO @ Thu, 08 Oct 2020 20:04:18: 28000000 INFO @ Thu, 08 Oct 2020 20:04:23: 25000000 INFO @ Thu, 08 Oct 2020 20:04:24: 22000000 INFO @ Thu, 08 Oct 2020 20:04:26: 29000000 INFO @ Thu, 08 Oct 2020 20:04:30: 26000000 INFO @ Thu, 08 Oct 2020 20:04:31: 23000000 INFO @ Thu, 08 Oct 2020 20:04:33: 30000000 INFO @ Thu, 08 Oct 2020 20:04:37: 27000000 INFO @ Thu, 08 Oct 2020 20:04:38: 24000000 INFO @ Thu, 08 Oct 2020 20:04:41: 31000000 INFO @ Thu, 08 Oct 2020 20:04:45: 25000000 INFO @ Thu, 08 Oct 2020 20:04:45: 28000000 INFO @ Thu, 08 Oct 2020 20:04:48: 32000000 INFO @ Thu, 08 Oct 2020 20:04:52: 26000000 INFO @ Thu, 08 Oct 2020 20:04:52: 29000000 INFO @ Thu, 08 Oct 2020 20:04:56: 33000000 INFO @ Thu, 08 Oct 2020 20:04:59: 27000000 INFO @ Thu, 08 Oct 2020 20:05:00: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 20:05:04: 34000000 INFO @ Thu, 08 Oct 2020 20:05:06: 28000000 INFO @ Thu, 08 Oct 2020 20:05:07: 31000000 INFO @ Thu, 08 Oct 2020 20:05:12: 35000000 INFO @ Thu, 08 Oct 2020 20:05:13: 29000000 INFO @ Thu, 08 Oct 2020 20:05:15: 32000000 INFO @ Thu, 08 Oct 2020 20:05:19: 36000000 INFO @ Thu, 08 Oct 2020 20:05:20: 30000000 INFO @ Thu, 08 Oct 2020 20:05:22: 33000000 INFO @ Thu, 08 Oct 2020 20:05:27: 37000000 INFO @ Thu, 08 Oct 2020 20:05:28: 31000000 INFO @ Thu, 08 Oct 2020 20:05:30: 34000000 INFO @ Thu, 08 Oct 2020 20:05:34: 32000000 INFO @ Thu, 08 Oct 2020 20:05:35: 38000000 INFO @ Thu, 08 Oct 2020 20:05:37: 35000000 INFO @ Thu, 08 Oct 2020 20:05:42: 33000000 INFO @ Thu, 08 Oct 2020 20:05:42: 39000000 INFO @ Thu, 08 Oct 2020 20:05:44: 36000000 INFO @ Thu, 08 Oct 2020 20:05:49: 34000000 INFO @ Thu, 08 Oct 2020 20:05:50: 40000000 INFO @ Thu, 08 Oct 2020 20:05:52: 37000000 INFO @ Thu, 08 Oct 2020 20:05:56: 35000000 INFO @ Thu, 08 Oct 2020 20:05:57: 41000000 INFO @ Thu, 08 Oct 2020 20:05:59: 38000000 INFO @ Thu, 08 Oct 2020 20:06:03: 36000000 INFO @ Thu, 08 Oct 2020 20:06:05: 42000000 INFO @ Thu, 08 Oct 2020 20:06:06: 39000000 INFO @ Thu, 08 Oct 2020 20:06:10: 37000000 INFO @ Thu, 08 Oct 2020 20:06:12: 43000000 INFO @ Thu, 08 Oct 2020 20:06:13: 40000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 20:06:17: 38000000 INFO @ Thu, 08 Oct 2020 20:06:20: 44000000 INFO @ Thu, 08 Oct 2020 20:06:21: 41000000 INFO @ Thu, 08 Oct 2020 20:06:23: 39000000 INFO @ Thu, 08 Oct 2020 20:06:27: 45000000 INFO @ Thu, 08 Oct 2020 20:06:28: 42000000 INFO @ Thu, 08 Oct 2020 20:06:30: 40000000 INFO @ Thu, 08 Oct 2020 20:06:34: 46000000 INFO @ Thu, 08 Oct 2020 20:06:35: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:06:35: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:06:35: #1 total tags in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:06:35: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:06:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:06:35: 43000000 INFO @ Thu, 08 Oct 2020 20:06:35: #1 tags after filtering in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:06:35: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:06:35: #1 finished! INFO @ Thu, 08 Oct 2020 20:06:35: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:06:35: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:06:38: 41000000 INFO @ Thu, 08 Oct 2020 20:06:38: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:06:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:06:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:06:42: 44000000 INFO @ Thu, 08 Oct 2020 20:06:44: 42000000 INFO @ Thu, 08 Oct 2020 20:06:49: 45000000 INFO @ Thu, 08 Oct 2020 20:06:51: 43000000 INFO @ Thu, 08 Oct 2020 20:06:56: 46000000 INFO @ Thu, 08 Oct 2020 20:06:57: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:06:57: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:06:57: #1 total tags in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:06:57: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:06:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:06:58: #1 tags after filtering in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:06:58: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:06:58: #1 finished! INFO @ Thu, 08 Oct 2020 20:06:58: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:06:58: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:06:58: 44000000 INFO @ Thu, 08 Oct 2020 20:07:01: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:07:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:07:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 20:07:04: 45000000 INFO @ Thu, 08 Oct 2020 20:07:10: 46000000 INFO @ Thu, 08 Oct 2020 20:07:10: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 20:07:10: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 20:07:10: #1 total tags in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:07:10: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 20:07:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 20:07:11: #1 tags after filtering in treatment: 46034908 INFO @ Thu, 08 Oct 2020 20:07:11: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 20:07:11: #1 finished! INFO @ Thu, 08 Oct 2020 20:07:11: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 20:07:11: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 20:07:14: #2 number of paired peaks: 0 WARNING @ Thu, 08 Oct 2020 20:07:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 20:07:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879252/SRX7879252.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling