Job ID = 14157840 SRX = SRX7879250 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45176912 spots for SRR11272865/SRR11272865.sra Written 45176912 spots for SRR11272865/SRR11272865.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157966 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:17 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2585086 (5.72%) aligned 0 times 35587140 (78.77%) aligned exactly 1 time 7004686 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:09:17 Overall time: 00:09:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7350674 / 42591826 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:30:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:30:25: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:30:25: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:30:31: 1000000 INFO @ Wed, 08 Dec 2021 11:30:36: 2000000 INFO @ Wed, 08 Dec 2021 11:30:42: 3000000 INFO @ Wed, 08 Dec 2021 11:30:47: 4000000 INFO @ Wed, 08 Dec 2021 11:30:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:30:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:30:55: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:30:55: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:30:58: 6000000 INFO @ Wed, 08 Dec 2021 11:31:01: 1000000 INFO @ Wed, 08 Dec 2021 11:31:04: 7000000 INFO @ Wed, 08 Dec 2021 11:31:07: 2000000 INFO @ Wed, 08 Dec 2021 11:31:10: 8000000 INFO @ Wed, 08 Dec 2021 11:31:13: 3000000 INFO @ Wed, 08 Dec 2021 11:31:16: 9000000 INFO @ Wed, 08 Dec 2021 11:31:19: 4000000 INFO @ Wed, 08 Dec 2021 11:31:22: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:31:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:31:25: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:31:25: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:31:25: 5000000 INFO @ Wed, 08 Dec 2021 11:31:29: 11000000 INFO @ Wed, 08 Dec 2021 11:31:32: 6000000 INFO @ Wed, 08 Dec 2021 11:31:33: 1000000 INFO @ Wed, 08 Dec 2021 11:31:35: 12000000 INFO @ Wed, 08 Dec 2021 11:31:39: 7000000 INFO @ Wed, 08 Dec 2021 11:31:40: 2000000 INFO @ Wed, 08 Dec 2021 11:31:42: 13000000 INFO @ Wed, 08 Dec 2021 11:31:46: 8000000 INFO @ Wed, 08 Dec 2021 11:31:48: 3000000 INFO @ Wed, 08 Dec 2021 11:31:49: 14000000 INFO @ Wed, 08 Dec 2021 11:31:53: 9000000 INFO @ Wed, 08 Dec 2021 11:31:55: 4000000 INFO @ Wed, 08 Dec 2021 11:31:56: 15000000 INFO @ Wed, 08 Dec 2021 11:32:00: 10000000 INFO @ Wed, 08 Dec 2021 11:32:03: 5000000 INFO @ Wed, 08 Dec 2021 11:32:03: 16000000 INFO @ Wed, 08 Dec 2021 11:32:06: 11000000 INFO @ Wed, 08 Dec 2021 11:32:09: 17000000 INFO @ Wed, 08 Dec 2021 11:32:10: 6000000 INFO @ Wed, 08 Dec 2021 11:32:13: 12000000 INFO @ Wed, 08 Dec 2021 11:32:16: 18000000 INFO @ Wed, 08 Dec 2021 11:32:17: 7000000 INFO @ Wed, 08 Dec 2021 11:32:20: 13000000 INFO @ Wed, 08 Dec 2021 11:32:23: 19000000 INFO @ Wed, 08 Dec 2021 11:32:24: 8000000 INFO @ Wed, 08 Dec 2021 11:32:27: 14000000 INFO @ Wed, 08 Dec 2021 11:32:30: 20000000 INFO @ Wed, 08 Dec 2021 11:32:31: 9000000 INFO @ Wed, 08 Dec 2021 11:32:34: 15000000 INFO @ Wed, 08 Dec 2021 11:32:37: 21000000 INFO @ Wed, 08 Dec 2021 11:32:38: 10000000 INFO @ Wed, 08 Dec 2021 11:32:41: 16000000 INFO @ Wed, 08 Dec 2021 11:32:44: 22000000 INFO @ Wed, 08 Dec 2021 11:32:46: 11000000 INFO @ Wed, 08 Dec 2021 11:32:47: 17000000 INFO @ Wed, 08 Dec 2021 11:32:50: 23000000 INFO @ Wed, 08 Dec 2021 11:32:53: 12000000 INFO @ Wed, 08 Dec 2021 11:32:54: 18000000 INFO @ Wed, 08 Dec 2021 11:32:57: 24000000 INFO @ Wed, 08 Dec 2021 11:33:00: 13000000 INFO @ Wed, 08 Dec 2021 11:33:01: 19000000 INFO @ Wed, 08 Dec 2021 11:33:04: 25000000 INFO @ Wed, 08 Dec 2021 11:33:07: 14000000 INFO @ Wed, 08 Dec 2021 11:33:08: 20000000 INFO @ Wed, 08 Dec 2021 11:33:11: 26000000 INFO @ Wed, 08 Dec 2021 11:33:14: 15000000 INFO @ Wed, 08 Dec 2021 11:33:15: 21000000 INFO @ Wed, 08 Dec 2021 11:33:18: 27000000 INFO @ Wed, 08 Dec 2021 11:33:21: 16000000 INFO @ Wed, 08 Dec 2021 11:33:22: 22000000 INFO @ Wed, 08 Dec 2021 11:33:25: 28000000 INFO @ Wed, 08 Dec 2021 11:33:28: 23000000 INFO @ Wed, 08 Dec 2021 11:33:29: 17000000 INFO @ Wed, 08 Dec 2021 11:33:32: 29000000 INFO @ Wed, 08 Dec 2021 11:33:35: 24000000 INFO @ Wed, 08 Dec 2021 11:33:36: 18000000 INFO @ Wed, 08 Dec 2021 11:33:39: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:33:42: 25000000 INFO @ Wed, 08 Dec 2021 11:33:43: 19000000 INFO @ Wed, 08 Dec 2021 11:33:45: 31000000 INFO @ Wed, 08 Dec 2021 11:33:49: 26000000 INFO @ Wed, 08 Dec 2021 11:33:50: 20000000 INFO @ Wed, 08 Dec 2021 11:33:52: 32000000 INFO @ Wed, 08 Dec 2021 11:33:56: 27000000 INFO @ Wed, 08 Dec 2021 11:33:57: 21000000 INFO @ Wed, 08 Dec 2021 11:33:59: 33000000 INFO @ Wed, 08 Dec 2021 11:34:03: 28000000 INFO @ Wed, 08 Dec 2021 11:34:04: 22000000 INFO @ Wed, 08 Dec 2021 11:34:06: 34000000 INFO @ Wed, 08 Dec 2021 11:34:09: 29000000 INFO @ Wed, 08 Dec 2021 11:34:11: 23000000 INFO @ Wed, 08 Dec 2021 11:34:13: 35000000 INFO @ Wed, 08 Dec 2021 11:34:14: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:34:14: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:34:14: #1 total tags in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:34:14: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:34:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:34:15: #1 tags after filtering in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:34:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:34:15: #1 finished! INFO @ Wed, 08 Dec 2021 11:34:15: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:34:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:34:16: 30000000 INFO @ Wed, 08 Dec 2021 11:34:17: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:34:17: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:34:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:34:19: 24000000 INFO @ Wed, 08 Dec 2021 11:34:22: 31000000 INFO @ Wed, 08 Dec 2021 11:34:26: 25000000 INFO @ Wed, 08 Dec 2021 11:34:29: 32000000 INFO @ Wed, 08 Dec 2021 11:34:33: 26000000 INFO @ Wed, 08 Dec 2021 11:34:36: 33000000 INFO @ Wed, 08 Dec 2021 11:34:40: 27000000 INFO @ Wed, 08 Dec 2021 11:34:42: 34000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 11:34:47: 28000000 INFO @ Wed, 08 Dec 2021 11:34:49: 35000000 INFO @ Wed, 08 Dec 2021 11:34:50: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:34:50: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:34:50: #1 total tags in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:34:50: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:34:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:34:51: #1 tags after filtering in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:34:51: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:34:51: #1 finished! INFO @ Wed, 08 Dec 2021 11:34:51: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:34:51: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:34:53: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:34:53: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:34:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:34:54: 29000000 INFO @ Wed, 08 Dec 2021 11:35:01: 30000000 INFO @ Wed, 08 Dec 2021 11:35:07: 31000000 INFO @ Wed, 08 Dec 2021 11:35:14: 32000000 INFO @ Wed, 08 Dec 2021 11:35:21: 33000000 INFO @ Wed, 08 Dec 2021 11:35:28: 34000000 INFO @ Wed, 08 Dec 2021 11:35:35: 35000000 INFO @ Wed, 08 Dec 2021 11:35:37: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:35:37: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:35:37: #1 total tags in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:35:37: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:35:37: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:35:37: #1 tags after filtering in treatment: 35241152 INFO @ Wed, 08 Dec 2021 11:35:37: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:35:37: #1 finished! INFO @ Wed, 08 Dec 2021 11:35:37: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:35:37: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:35:39: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:35:39: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:35:39: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling