Job ID = 10165642 SRX = SRX7879250 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 45176912 spots for SRR11272865/SRR11272865.sra Written 45176912 spots for SRR11272865/SRR11272865.sra fastq に変換しました。 bowtie でマッピング中... Your job 10165927 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:09 45176912 reads; of these: 45176912 (100.00%) were unpaired; of these: 2585086 (5.72%) aligned 0 times 35587140 (78.77%) aligned exactly 1 time 7004686 (15.51%) aligned >1 times 94.28% overall alignment rate Time searching: 00:09:09 Overall time: 00:09:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7350674 / 42591826 = 0.1726 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:27: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:27: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:32: 1000000 INFO @ Thu, 08 Oct 2020 19:51:37: 2000000 INFO @ Thu, 08 Oct 2020 19:51:43: 3000000 INFO @ Thu, 08 Oct 2020 19:51:48: 4000000 INFO @ Thu, 08 Oct 2020 19:51:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:51:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:51:56: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:51:56: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:51:59: 6000000 INFO @ Thu, 08 Oct 2020 19:52:02: 1000000 INFO @ Thu, 08 Oct 2020 19:52:05: 7000000 INFO @ Thu, 08 Oct 2020 19:52:08: 2000000 INFO @ Thu, 08 Oct 2020 19:52:10: 8000000 INFO @ Thu, 08 Oct 2020 19:52:14: 3000000 INFO @ Thu, 08 Oct 2020 19:52:16: 9000000 INFO @ Thu, 08 Oct 2020 19:52:19: 4000000 INFO @ Thu, 08 Oct 2020 19:52:22: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 08 Oct 2020 19:52:25: 5000000 INFO @ Thu, 08 Oct 2020 19:52:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 08 Oct 2020 19:52:26: #1 read tag files... INFO @ Thu, 08 Oct 2020 19:52:26: #1 read treatment tags... INFO @ Thu, 08 Oct 2020 19:52:28: 11000000 INFO @ Thu, 08 Oct 2020 19:52:31: 6000000 INFO @ Thu, 08 Oct 2020 19:52:32: 1000000 INFO @ Thu, 08 Oct 2020 19:52:34: 12000000 INFO @ Thu, 08 Oct 2020 19:52:37: 7000000 INFO @ Thu, 08 Oct 2020 19:52:38: 2000000 INFO @ Thu, 08 Oct 2020 19:52:39: 13000000 INFO @ Thu, 08 Oct 2020 19:52:42: 8000000 INFO @ Thu, 08 Oct 2020 19:52:44: 3000000 INFO @ Thu, 08 Oct 2020 19:52:45: 14000000 INFO @ Thu, 08 Oct 2020 19:52:48: 9000000 INFO @ Thu, 08 Oct 2020 19:52:50: 4000000 INFO @ Thu, 08 Oct 2020 19:52:51: 15000000 INFO @ Thu, 08 Oct 2020 19:52:54: 10000000 INFO @ Thu, 08 Oct 2020 19:52:56: 5000000 INFO @ Thu, 08 Oct 2020 19:52:57: 16000000 INFO @ Thu, 08 Oct 2020 19:52:59: 11000000 INFO @ Thu, 08 Oct 2020 19:53:02: 6000000 INFO @ Thu, 08 Oct 2020 19:53:02: 17000000 INFO @ Thu, 08 Oct 2020 19:53:05: 12000000 INFO @ Thu, 08 Oct 2020 19:53:08: 7000000 INFO @ Thu, 08 Oct 2020 19:53:08: 18000000 INFO @ Thu, 08 Oct 2020 19:53:11: 13000000 INFO @ Thu, 08 Oct 2020 19:53:14: 19000000 INFO @ Thu, 08 Oct 2020 19:53:14: 8000000 INFO @ Thu, 08 Oct 2020 19:53:17: 14000000 INFO @ Thu, 08 Oct 2020 19:53:19: 20000000 INFO @ Thu, 08 Oct 2020 19:53:21: 9000000 INFO @ Thu, 08 Oct 2020 19:53:22: 15000000 INFO @ Thu, 08 Oct 2020 19:53:25: 21000000 INFO @ Thu, 08 Oct 2020 19:53:27: 10000000 INFO @ Thu, 08 Oct 2020 19:53:28: 16000000 INFO @ Thu, 08 Oct 2020 19:53:31: 22000000 INFO @ Thu, 08 Oct 2020 19:53:34: 11000000 INFO @ Thu, 08 Oct 2020 19:53:34: 17000000 INFO @ Thu, 08 Oct 2020 19:53:37: 23000000 INFO @ Thu, 08 Oct 2020 19:53:39: 18000000 INFO @ Thu, 08 Oct 2020 19:53:40: 12000000 INFO @ Thu, 08 Oct 2020 19:53:42: 24000000 INFO @ Thu, 08 Oct 2020 19:53:45: 19000000 INFO @ Thu, 08 Oct 2020 19:53:47: 13000000 INFO @ Thu, 08 Oct 2020 19:53:48: 25000000 INFO @ Thu, 08 Oct 2020 19:53:51: 20000000 INFO @ Thu, 08 Oct 2020 19:53:53: 14000000 INFO @ Thu, 08 Oct 2020 19:53:54: 26000000 INFO @ Thu, 08 Oct 2020 19:53:56: 21000000 INFO @ Thu, 08 Oct 2020 19:53:59: 27000000 INFO @ Thu, 08 Oct 2020 19:54:00: 15000000 INFO @ Thu, 08 Oct 2020 19:54:02: 22000000 INFO @ Thu, 08 Oct 2020 19:54:05: 28000000 INFO @ Thu, 08 Oct 2020 19:54:07: 16000000 INFO @ Thu, 08 Oct 2020 19:54:08: 23000000 INFO @ Thu, 08 Oct 2020 19:54:11: 29000000 INFO @ Thu, 08 Oct 2020 19:54:13: 17000000 INFO @ Thu, 08 Oct 2020 19:54:14: 24000000 INFO @ Thu, 08 Oct 2020 19:54:17: 30000000 INFO @ Thu, 08 Oct 2020 19:54:19: 25000000 INFO @ Thu, 08 Oct 2020 19:54:20: 18000000 INFO @ Thu, 08 Oct 2020 19:54:22: 31000000 INFO @ Thu, 08 Oct 2020 19:54:25: 26000000 INFO @ Thu, 08 Oct 2020 19:54:27: 19000000 INFO @ Thu, 08 Oct 2020 19:54:28: 32000000 INFO @ Thu, 08 Oct 2020 19:54:31: 27000000 INFO @ Thu, 08 Oct 2020 19:54:34: 20000000 INFO @ Thu, 08 Oct 2020 19:54:34: 33000000 INFO @ Thu, 08 Oct 2020 19:54:36: 28000000 INFO @ Thu, 08 Oct 2020 19:54:40: 34000000 INFO @ Thu, 08 Oct 2020 19:54:40: 21000000 INFO @ Thu, 08 Oct 2020 19:54:42: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 08 Oct 2020 19:54:46: 35000000 INFO @ Thu, 08 Oct 2020 19:54:47: 22000000 INFO @ Thu, 08 Oct 2020 19:54:47: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:54:47: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:54:47: #1 total tags in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:54:47: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:54:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:54:48: #1 tags after filtering in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:54:48: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:54:48: #1 finished! INFO @ Thu, 08 Oct 2020 19:54:48: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:54:48: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:54:48: 30000000 INFO @ Thu, 08 Oct 2020 19:54:50: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:54:50: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:54:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:54:53: 31000000 INFO @ Thu, 08 Oct 2020 19:54:54: 23000000 INFO @ Thu, 08 Oct 2020 19:54:59: 32000000 INFO @ Thu, 08 Oct 2020 19:55:01: 24000000 INFO @ Thu, 08 Oct 2020 19:55:05: 33000000 INFO @ Thu, 08 Oct 2020 19:55:08: 25000000 INFO @ Thu, 08 Oct 2020 19:55:10: 34000000 INFO @ Thu, 08 Oct 2020 19:55:15: 26000000 INFO @ Thu, 08 Oct 2020 19:55:16: 35000000 INFO @ Thu, 08 Oct 2020 19:55:17: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:55:17: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:55:17: #1 total tags in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:55:17: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:55:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:55:18: #1 tags after filtering in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:55:18: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:55:18: #1 finished! INFO @ Thu, 08 Oct 2020 19:55:18: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:55:18: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:55:20: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:55:20: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:55:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Thu, 08 Oct 2020 19:55:22: 27000000 INFO @ Thu, 08 Oct 2020 19:55:29: 28000000 INFO @ Thu, 08 Oct 2020 19:55:36: 29000000 INFO @ Thu, 08 Oct 2020 19:55:43: 30000000 BigWig に変換しました。 INFO @ Thu, 08 Oct 2020 19:55:50: 31000000 INFO @ Thu, 08 Oct 2020 19:55:58: 32000000 INFO @ Thu, 08 Oct 2020 19:56:05: 33000000 INFO @ Thu, 08 Oct 2020 19:56:11: 34000000 INFO @ Thu, 08 Oct 2020 19:56:18: 35000000 INFO @ Thu, 08 Oct 2020 19:56:20: #1 tag size is determined as 50 bps INFO @ Thu, 08 Oct 2020 19:56:20: #1 tag size = 50 INFO @ Thu, 08 Oct 2020 19:56:20: #1 total tags in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:56:20: #1 user defined the maximum tags... INFO @ Thu, 08 Oct 2020 19:56:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 08 Oct 2020 19:56:20: #1 tags after filtering in treatment: 35241152 INFO @ Thu, 08 Oct 2020 19:56:20: #1 Redundant rate of treatment: 0.00 INFO @ Thu, 08 Oct 2020 19:56:20: #1 finished! INFO @ Thu, 08 Oct 2020 19:56:20: #2 Build Peak Model... INFO @ Thu, 08 Oct 2020 19:56:20: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 08 Oct 2020 19:56:22: #2 number of paired peaks: 37 WARNING @ Thu, 08 Oct 2020 19:56:22: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 08 Oct 2020 19:56:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879250/SRX7879250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling