Job ID = 14157838 SRX = SRX7879248 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 43398663 spots for SRR11272863/SRR11272863.sra Written 43398663 spots for SRR11272863/SRR11272863.sra fastq に変換しました。 bowtie でマッピング中... Your job 14157974 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:43 43398663 reads; of these: 43398663 (100.00%) were unpaired; of these: 700587 (1.61%) aligned 0 times 35754396 (82.39%) aligned exactly 1 time 6943680 (16.00%) aligned >1 times 98.39% overall alignment rate Time searching: 00:10:43 Overall time: 00:10:43 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 7318541 / 42698076 = 0.1714 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:36:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:36:23: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:36:23: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:36:30: 1000000 INFO @ Wed, 08 Dec 2021 11:36:37: 2000000 INFO @ Wed, 08 Dec 2021 11:36:43: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:36:50: 4000000 INFO @ Wed, 08 Dec 2021 11:36:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:36:52: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:36:52: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:36:57: 5000000 INFO @ Wed, 08 Dec 2021 11:37:00: 1000000 INFO @ Wed, 08 Dec 2021 11:37:04: 6000000 INFO @ Wed, 08 Dec 2021 11:37:08: 2000000 INFO @ Wed, 08 Dec 2021 11:37:11: 7000000 INFO @ Wed, 08 Dec 2021 11:37:17: 3000000 INFO @ Wed, 08 Dec 2021 11:37:18: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 11:37:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 11:37:22: #1 read tag files... INFO @ Wed, 08 Dec 2021 11:37:22: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 11:37:25: 9000000 INFO @ Wed, 08 Dec 2021 11:37:25: 4000000 INFO @ Wed, 08 Dec 2021 11:37:30: 1000000 INFO @ Wed, 08 Dec 2021 11:37:32: 10000000 INFO @ Wed, 08 Dec 2021 11:37:33: 5000000 INFO @ Wed, 08 Dec 2021 11:37:37: 2000000 INFO @ Wed, 08 Dec 2021 11:37:38: 11000000 INFO @ Wed, 08 Dec 2021 11:37:42: 6000000 INFO @ Wed, 08 Dec 2021 11:37:45: 3000000 INFO @ Wed, 08 Dec 2021 11:37:45: 12000000 INFO @ Wed, 08 Dec 2021 11:37:50: 7000000 INFO @ Wed, 08 Dec 2021 11:37:52: 4000000 INFO @ Wed, 08 Dec 2021 11:37:52: 13000000 INFO @ Wed, 08 Dec 2021 11:37:58: 8000000 INFO @ Wed, 08 Dec 2021 11:37:59: 14000000 INFO @ Wed, 08 Dec 2021 11:37:59: 5000000 INFO @ Wed, 08 Dec 2021 11:38:06: 15000000 INFO @ Wed, 08 Dec 2021 11:38:06: 9000000 INFO @ Wed, 08 Dec 2021 11:38:07: 6000000 INFO @ Wed, 08 Dec 2021 11:38:13: 16000000 INFO @ Wed, 08 Dec 2021 11:38:14: 7000000 INFO @ Wed, 08 Dec 2021 11:38:15: 10000000 INFO @ Wed, 08 Dec 2021 11:38:19: 17000000 INFO @ Wed, 08 Dec 2021 11:38:22: 8000000 INFO @ Wed, 08 Dec 2021 11:38:23: 11000000 INFO @ Wed, 08 Dec 2021 11:38:26: 18000000 INFO @ Wed, 08 Dec 2021 11:38:29: 9000000 INFO @ Wed, 08 Dec 2021 11:38:31: 12000000 INFO @ Wed, 08 Dec 2021 11:38:33: 19000000 INFO @ Wed, 08 Dec 2021 11:38:36: 10000000 INFO @ Wed, 08 Dec 2021 11:38:39: 13000000 INFO @ Wed, 08 Dec 2021 11:38:40: 20000000 INFO @ Wed, 08 Dec 2021 11:38:44: 11000000 INFO @ Wed, 08 Dec 2021 11:38:47: 21000000 INFO @ Wed, 08 Dec 2021 11:38:48: 14000000 INFO @ Wed, 08 Dec 2021 11:38:51: 12000000 INFO @ Wed, 08 Dec 2021 11:38:54: 22000000 INFO @ Wed, 08 Dec 2021 11:38:56: 15000000 INFO @ Wed, 08 Dec 2021 11:38:58: 13000000 INFO @ Wed, 08 Dec 2021 11:39:01: 23000000 INFO @ Wed, 08 Dec 2021 11:39:04: 16000000 INFO @ Wed, 08 Dec 2021 11:39:06: 14000000 INFO @ Wed, 08 Dec 2021 11:39:08: 24000000 INFO @ Wed, 08 Dec 2021 11:39:12: 17000000 INFO @ Wed, 08 Dec 2021 11:39:13: 15000000 INFO @ Wed, 08 Dec 2021 11:39:15: 25000000 INFO @ Wed, 08 Dec 2021 11:39:21: 16000000 INFO @ Wed, 08 Dec 2021 11:39:21: 18000000 INFO @ Wed, 08 Dec 2021 11:39:22: 26000000 INFO @ Wed, 08 Dec 2021 11:39:28: 17000000 INFO @ Wed, 08 Dec 2021 11:39:29: 27000000 INFO @ Wed, 08 Dec 2021 11:39:29: 19000000 INFO @ Wed, 08 Dec 2021 11:39:35: 18000000 INFO @ Wed, 08 Dec 2021 11:39:35: 28000000 INFO @ Wed, 08 Dec 2021 11:39:37: 20000000 INFO @ Wed, 08 Dec 2021 11:39:42: 29000000 INFO @ Wed, 08 Dec 2021 11:39:43: 19000000 INFO @ Wed, 08 Dec 2021 11:39:46: 21000000 INFO @ Wed, 08 Dec 2021 11:39:50: 30000000 INFO @ Wed, 08 Dec 2021 11:39:50: 20000000 INFO @ Wed, 08 Dec 2021 11:39:54: 22000000 INFO @ Wed, 08 Dec 2021 11:39:56: 31000000 INFO @ Wed, 08 Dec 2021 11:39:57: 21000000 INFO @ Wed, 08 Dec 2021 11:40:02: 23000000 INFO @ Wed, 08 Dec 2021 11:40:03: 32000000 INFO @ Wed, 08 Dec 2021 11:40:05: 22000000 INFO @ Wed, 08 Dec 2021 11:40:10: 33000000 INFO @ Wed, 08 Dec 2021 11:40:10: 24000000 INFO @ Wed, 08 Dec 2021 11:40:12: 23000000 INFO @ Wed, 08 Dec 2021 11:40:17: 34000000 INFO @ Wed, 08 Dec 2021 11:40:19: 25000000 INFO @ Wed, 08 Dec 2021 11:40:19: 24000000 INFO @ Wed, 08 Dec 2021 11:40:24: 35000000 INFO @ Wed, 08 Dec 2021 11:40:27: 25000000 INFO @ Wed, 08 Dec 2021 11:40:27: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:40:27: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:40:27: #1 total tags in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:40:27: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:40:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:40:27: 26000000 INFO @ Wed, 08 Dec 2021 11:40:27: #1 tags after filtering in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:40:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:40:27: #1 finished! INFO @ Wed, 08 Dec 2021 11:40:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:40:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:40:30: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:40:30: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:40:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:40:34: 26000000 INFO @ Wed, 08 Dec 2021 11:40:35: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 11:40:41: 27000000 INFO @ Wed, 08 Dec 2021 11:40:43: 28000000 INFO @ Wed, 08 Dec 2021 11:40:49: 28000000 INFO @ Wed, 08 Dec 2021 11:40:52: 29000000 INFO @ Wed, 08 Dec 2021 11:40:56: 29000000 INFO @ Wed, 08 Dec 2021 11:41:00: 30000000 INFO @ Wed, 08 Dec 2021 11:41:03: 30000000 INFO @ Wed, 08 Dec 2021 11:41:08: 31000000 INFO @ Wed, 08 Dec 2021 11:41:11: 31000000 INFO @ Wed, 08 Dec 2021 11:41:16: 32000000 INFO @ Wed, 08 Dec 2021 11:41:18: 32000000 INFO @ Wed, 08 Dec 2021 11:41:24: 33000000 INFO @ Wed, 08 Dec 2021 11:41:25: 33000000 INFO @ Wed, 08 Dec 2021 11:41:32: 34000000 INFO @ Wed, 08 Dec 2021 11:41:33: 34000000 INFO @ Wed, 08 Dec 2021 11:41:40: 35000000 INFO @ Wed, 08 Dec 2021 11:41:41: 35000000 INFO @ Wed, 08 Dec 2021 11:41:43: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:41:43: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:41:43: #1 total tags in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:41:43: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:41:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:41:44: #1 tags after filtering in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:41:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:41:44: #1 finished! INFO @ Wed, 08 Dec 2021 11:41:44: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:41:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:41:44: #1 tag size is determined as 50 bps INFO @ Wed, 08 Dec 2021 11:41:44: #1 tag size = 50 INFO @ Wed, 08 Dec 2021 11:41:44: #1 total tags in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:41:44: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 11:41:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 11:41:45: #1 tags after filtering in treatment: 35379535 INFO @ Wed, 08 Dec 2021 11:41:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 11:41:45: #1 finished! INFO @ Wed, 08 Dec 2021 11:41:45: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 11:41:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 11:41:46: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:41:46: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:41:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 11:41:48: #2 number of paired peaks: 37 WARNING @ Wed, 08 Dec 2021 11:41:48: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 11:41:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7879248/SRX7879248.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。