Job ID = 14158260 SRX = SRX7739527 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 53869301 spots for SRR11100931/SRR11100931.sra Written 53869301 spots for SRR11100931/SRR11100931.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158792 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:43 53869301 reads; of these: 53869301 (100.00%) were unpaired; of these: 189998 (0.35%) aligned 0 times 44488696 (82.59%) aligned exactly 1 time 9190607 (17.06%) aligned >1 times 99.65% overall alignment rate Time searching: 00:13:44 Overall time: 00:13:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 24 files... [bam_rmdupse_core] 10421266 / 53679303 = 0.1941 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 15:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 15:54:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 15:54:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 15:54:22: 1000000 INFO @ Wed, 08 Dec 2021 15:54:29: 2000000 INFO @ Wed, 08 Dec 2021 15:54:36: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 15:54:43: 4000000 INFO @ Wed, 08 Dec 2021 15:54:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 15:54:45: #1 read tag files... INFO @ Wed, 08 Dec 2021 15:54:45: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 15:54:51: 5000000 INFO @ Wed, 08 Dec 2021 15:54:53: 1000000 INFO @ Wed, 08 Dec 2021 15:54:58: 6000000 INFO @ Wed, 08 Dec 2021 15:55:00: 2000000 INFO @ Wed, 08 Dec 2021 15:55:05: 7000000 INFO @ Wed, 08 Dec 2021 15:55:07: 3000000 INFO @ Wed, 08 Dec 2021 15:55:13: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 15:55:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 15:55:15: #1 read tag files... INFO @ Wed, 08 Dec 2021 15:55:15: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 15:55:15: 4000000 INFO @ Wed, 08 Dec 2021 15:55:20: 9000000 INFO @ Wed, 08 Dec 2021 15:55:22: 1000000 INFO @ Wed, 08 Dec 2021 15:55:23: 5000000 INFO @ Wed, 08 Dec 2021 15:55:28: 10000000 INFO @ Wed, 08 Dec 2021 15:55:30: 2000000 INFO @ Wed, 08 Dec 2021 15:55:30: 6000000 INFO @ Wed, 08 Dec 2021 15:55:35: 11000000 INFO @ Wed, 08 Dec 2021 15:55:38: 3000000 INFO @ Wed, 08 Dec 2021 15:55:38: 7000000 INFO @ Wed, 08 Dec 2021 15:55:42: 12000000 INFO @ Wed, 08 Dec 2021 15:55:45: 4000000 INFO @ Wed, 08 Dec 2021 15:55:46: 8000000 INFO @ Wed, 08 Dec 2021 15:55:50: 13000000 INFO @ Wed, 08 Dec 2021 15:55:53: 5000000 INFO @ Wed, 08 Dec 2021 15:55:53: 9000000 INFO @ Wed, 08 Dec 2021 15:55:57: 14000000 INFO @ Wed, 08 Dec 2021 15:56:00: 10000000 INFO @ Wed, 08 Dec 2021 15:56:00: 6000000 INFO @ Wed, 08 Dec 2021 15:56:04: 15000000 INFO @ Wed, 08 Dec 2021 15:56:08: 11000000 INFO @ Wed, 08 Dec 2021 15:56:08: 7000000 INFO @ Wed, 08 Dec 2021 15:56:12: 16000000 INFO @ Wed, 08 Dec 2021 15:56:15: 12000000 INFO @ Wed, 08 Dec 2021 15:56:16: 8000000 INFO @ Wed, 08 Dec 2021 15:56:19: 17000000 INFO @ Wed, 08 Dec 2021 15:56:23: 13000000 INFO @ Wed, 08 Dec 2021 15:56:23: 9000000 INFO @ Wed, 08 Dec 2021 15:56:26: 18000000 INFO @ Wed, 08 Dec 2021 15:56:30: 14000000 INFO @ Wed, 08 Dec 2021 15:56:30: 10000000 INFO @ Wed, 08 Dec 2021 15:56:33: 19000000 INFO @ Wed, 08 Dec 2021 15:56:37: 15000000 INFO @ Wed, 08 Dec 2021 15:56:38: 11000000 INFO @ Wed, 08 Dec 2021 15:56:41: 20000000 INFO @ Wed, 08 Dec 2021 15:56:45: 16000000 INFO @ Wed, 08 Dec 2021 15:56:45: 12000000 INFO @ Wed, 08 Dec 2021 15:56:48: 21000000 INFO @ Wed, 08 Dec 2021 15:56:52: 17000000 INFO @ Wed, 08 Dec 2021 15:56:53: 13000000 INFO @ Wed, 08 Dec 2021 15:56:55: 22000000 INFO @ Wed, 08 Dec 2021 15:56:59: 18000000 INFO @ Wed, 08 Dec 2021 15:57:00: 14000000 INFO @ Wed, 08 Dec 2021 15:57:03: 23000000 INFO @ Wed, 08 Dec 2021 15:57:06: 19000000 INFO @ Wed, 08 Dec 2021 15:57:07: 15000000 INFO @ Wed, 08 Dec 2021 15:57:10: 24000000 INFO @ Wed, 08 Dec 2021 15:57:14: 20000000 INFO @ Wed, 08 Dec 2021 15:57:15: 16000000 INFO @ Wed, 08 Dec 2021 15:57:17: 25000000 INFO @ Wed, 08 Dec 2021 15:57:21: 21000000 INFO @ Wed, 08 Dec 2021 15:57:22: 17000000 INFO @ Wed, 08 Dec 2021 15:57:25: 26000000 INFO @ Wed, 08 Dec 2021 15:57:28: 22000000 INFO @ Wed, 08 Dec 2021 15:57:29: 18000000 INFO @ Wed, 08 Dec 2021 15:57:32: 27000000 INFO @ Wed, 08 Dec 2021 15:57:35: 23000000 INFO @ Wed, 08 Dec 2021 15:57:36: 19000000 INFO @ Wed, 08 Dec 2021 15:57:39: 28000000 INFO @ Wed, 08 Dec 2021 15:57:43: 24000000 INFO @ Wed, 08 Dec 2021 15:57:44: 20000000 INFO @ Wed, 08 Dec 2021 15:57:46: 29000000 INFO @ Wed, 08 Dec 2021 15:57:50: 25000000 INFO @ Wed, 08 Dec 2021 15:57:51: 21000000 INFO @ Wed, 08 Dec 2021 15:57:54: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 08 Dec 2021 15:57:57: 26000000 INFO @ Wed, 08 Dec 2021 15:57:58: 22000000 INFO @ Wed, 08 Dec 2021 15:58:01: 31000000 INFO @ Wed, 08 Dec 2021 15:58:05: 27000000 INFO @ Wed, 08 Dec 2021 15:58:06: 23000000 INFO @ Wed, 08 Dec 2021 15:58:09: 32000000 INFO @ Wed, 08 Dec 2021 15:58:12: 28000000 INFO @ Wed, 08 Dec 2021 15:58:13: 24000000 INFO @ Wed, 08 Dec 2021 15:58:16: 33000000 INFO @ Wed, 08 Dec 2021 15:58:19: 29000000 INFO @ Wed, 08 Dec 2021 15:58:21: 25000000 INFO @ Wed, 08 Dec 2021 15:58:23: 34000000 INFO @ Wed, 08 Dec 2021 15:58:26: 30000000 INFO @ Wed, 08 Dec 2021 15:58:28: 26000000 INFO @ Wed, 08 Dec 2021 15:58:31: 35000000 INFO @ Wed, 08 Dec 2021 15:58:34: 31000000 INFO @ Wed, 08 Dec 2021 15:58:35: 27000000 INFO @ Wed, 08 Dec 2021 15:58:38: 36000000 INFO @ Wed, 08 Dec 2021 15:58:41: 32000000 INFO @ Wed, 08 Dec 2021 15:58:43: 28000000 INFO @ Wed, 08 Dec 2021 15:58:45: 37000000 INFO @ Wed, 08 Dec 2021 15:58:48: 33000000 INFO @ Wed, 08 Dec 2021 15:58:50: 29000000 INFO @ Wed, 08 Dec 2021 15:58:53: 38000000 INFO @ Wed, 08 Dec 2021 15:58:55: 34000000 INFO @ Wed, 08 Dec 2021 15:58:57: 30000000 INFO @ Wed, 08 Dec 2021 15:59:00: 39000000 INFO @ Wed, 08 Dec 2021 15:59:02: 35000000 BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 15:59:04: 31000000 INFO @ Wed, 08 Dec 2021 15:59:07: 40000000 INFO @ Wed, 08 Dec 2021 15:59:10: 36000000 INFO @ Wed, 08 Dec 2021 15:59:12: 32000000 INFO @ Wed, 08 Dec 2021 15:59:15: 41000000 INFO @ Wed, 08 Dec 2021 15:59:17: 37000000 INFO @ Wed, 08 Dec 2021 15:59:19: 33000000 INFO @ Wed, 08 Dec 2021 15:59:22: 42000000 INFO @ Wed, 08 Dec 2021 15:59:24: 38000000 INFO @ Wed, 08 Dec 2021 15:59:26: 34000000 INFO @ Wed, 08 Dec 2021 15:59:29: 43000000 INFO @ Wed, 08 Dec 2021 15:59:31: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 15:59:31: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 15:59:31: #1 total tags in treatment: 43258037 INFO @ Wed, 08 Dec 2021 15:59:31: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 15:59:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 15:59:31: 39000000 INFO @ Wed, 08 Dec 2021 15:59:32: #1 tags after filtering in treatment: 43258037 INFO @ Wed, 08 Dec 2021 15:59:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 15:59:32: #1 finished! INFO @ Wed, 08 Dec 2021 15:59:32: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 15:59:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 15:59:33: 35000000 INFO @ Wed, 08 Dec 2021 15:59:34: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 15:59:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 15:59:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 15:59:38: 40000000 INFO @ Wed, 08 Dec 2021 15:59:40: 36000000 INFO @ Wed, 08 Dec 2021 15:59:46: 41000000 INFO @ Wed, 08 Dec 2021 15:59:47: 37000000 INFO @ Wed, 08 Dec 2021 15:59:53: 42000000 INFO @ Wed, 08 Dec 2021 15:59:54: 38000000 INFO @ Wed, 08 Dec 2021 16:00:00: 43000000 INFO @ Wed, 08 Dec 2021 16:00:01: 39000000 INFO @ Wed, 08 Dec 2021 16:00:01: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 16:00:01: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 16:00:01: #1 total tags in treatment: 43258037 INFO @ Wed, 08 Dec 2021 16:00:01: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 16:00:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 16:00:02: #1 tags after filtering in treatment: 43258037 INFO @ Wed, 08 Dec 2021 16:00:02: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 16:00:02: #1 finished! INFO @ Wed, 08 Dec 2021 16:00:02: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 16:00:02: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 16:00:05: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 16:00:05: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 16:00:05: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 08 Dec 2021 16:00:08: 40000000 INFO @ Wed, 08 Dec 2021 16:00:15: 41000000 INFO @ Wed, 08 Dec 2021 16:00:22: 42000000 INFO @ Wed, 08 Dec 2021 16:00:29: 43000000 INFO @ Wed, 08 Dec 2021 16:00:30: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 16:00:30: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 16:00:30: #1 total tags in treatment: 43258037 INFO @ Wed, 08 Dec 2021 16:00:30: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 16:00:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 16:00:31: #1 tags after filtering in treatment: 43258037 INFO @ Wed, 08 Dec 2021 16:00:31: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 16:00:31: #1 finished! INFO @ Wed, 08 Dec 2021 16:00:31: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 16:00:31: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 16:00:34: #2 number of paired peaks: 0 WARNING @ Wed, 08 Dec 2021 16:00:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 08 Dec 2021 16:00:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX7739527/SRX7739527.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling