Job ID = 6626352 SRX = SRX7262193 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 10852432 spots for SRR10581816/SRR10581816.sra Written 10852432 spots for SRR10581816/SRR10581816.sra fastq に変換しました。 bowtie でマッピング中... Your job 6626434 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:12 10852432 reads; of these: 10852432 (100.00%) were unpaired; of these: 749870 (6.91%) aligned 0 times 7940710 (73.17%) aligned exactly 1 time 2161852 (19.92%) aligned >1 times 93.09% overall alignment rate Time searching: 00:02:12 Overall time: 00:02:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 1071549 / 10102562 = 0.1061 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:00:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:00:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:00:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:00:37: 1000000 INFO @ Tue, 14 Jul 2020 07:00:43: 2000000 INFO @ Tue, 14 Jul 2020 07:00:50: 3000000 INFO @ Tue, 14 Jul 2020 07:00:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:01:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:01:01: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:01:01: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:01:04: 5000000 INFO @ Tue, 14 Jul 2020 07:01:08: 1000000 INFO @ Tue, 14 Jul 2020 07:01:12: 6000000 INFO @ Tue, 14 Jul 2020 07:01:16: 2000000 INFO @ Tue, 14 Jul 2020 07:01:19: 7000000 INFO @ Tue, 14 Jul 2020 07:01:23: 3000000 INFO @ Tue, 14 Jul 2020 07:01:26: 8000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 14 Jul 2020 07:01:29: 4000000 INFO @ Tue, 14 Jul 2020 07:01:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 14 Jul 2020 07:01:31: #1 read tag files... INFO @ Tue, 14 Jul 2020 07:01:31: #1 read treatment tags... INFO @ Tue, 14 Jul 2020 07:01:33: 9000000 INFO @ Tue, 14 Jul 2020 07:01:33: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:01:33: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:01:33: #1 total tags in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:01:33: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:01:34: #1 tags after filtering in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:01:34: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:01:34: #1 finished! INFO @ Tue, 14 Jul 2020 07:01:34: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:01:34: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:01:34: #2 number of paired peaks: 402 WARNING @ Tue, 14 Jul 2020 07:01:34: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 14 Jul 2020 07:01:34: start model_add_line... INFO @ Tue, 14 Jul 2020 07:01:34: start X-correlation... INFO @ Tue, 14 Jul 2020 07:01:34: end of X-cor INFO @ Tue, 14 Jul 2020 07:01:34: #2 finished! INFO @ Tue, 14 Jul 2020 07:01:34: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 07:01:34: #2 alternative fragment length(s) may be 2,46,541 bps INFO @ Tue, 14 Jul 2020 07:01:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05_model.r WARNING @ Tue, 14 Jul 2020 07:01:34: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 07:01:34: #2 You may need to consider one of the other alternative d(s): 2,46,541 WARNING @ Tue, 14 Jul 2020 07:01:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 07:01:34: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:01:34: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:01:36: 5000000 INFO @ Tue, 14 Jul 2020 07:01:38: 1000000 INFO @ Tue, 14 Jul 2020 07:01:43: 6000000 INFO @ Tue, 14 Jul 2020 07:01:45: 2000000 INFO @ Tue, 14 Jul 2020 07:01:50: 7000000 INFO @ Tue, 14 Jul 2020 07:01:52: 3000000 INFO @ Tue, 14 Jul 2020 07:01:52: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:01:57: 8000000 INFO @ Tue, 14 Jul 2020 07:01:59: 4000000 INFO @ Tue, 14 Jul 2020 07:02:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05_peaks.xls INFO @ Tue, 14 Jul 2020 07:02:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:02:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.05_summits.bed INFO @ Tue, 14 Jul 2020 07:02:01: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (693 records, 4 fields): 35 millis CompletedMACS2peakCalling INFO @ Tue, 14 Jul 2020 07:02:04: 9000000 INFO @ Tue, 14 Jul 2020 07:02:04: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:02:04: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:02:04: #1 total tags in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:02:04: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:02:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:02:04: #1 tags after filtering in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:02:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:02:04: #1 finished! INFO @ Tue, 14 Jul 2020 07:02:04: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:02:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:02:05: #2 number of paired peaks: 402 WARNING @ Tue, 14 Jul 2020 07:02:05: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 14 Jul 2020 07:02:05: start model_add_line... INFO @ Tue, 14 Jul 2020 07:02:05: start X-correlation... INFO @ Tue, 14 Jul 2020 07:02:05: end of X-cor INFO @ Tue, 14 Jul 2020 07:02:05: #2 finished! INFO @ Tue, 14 Jul 2020 07:02:05: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 07:02:05: #2 alternative fragment length(s) may be 2,46,541 bps INFO @ Tue, 14 Jul 2020 07:02:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10_model.r WARNING @ Tue, 14 Jul 2020 07:02:05: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 07:02:05: #2 You may need to consider one of the other alternative d(s): 2,46,541 WARNING @ Tue, 14 Jul 2020 07:02:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 07:02:05: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:02:05: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:02:06: 5000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 14 Jul 2020 07:02:12: 6000000 INFO @ Tue, 14 Jul 2020 07:02:19: 7000000 INFO @ Tue, 14 Jul 2020 07:02:22: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:02:26: 8000000 INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10_peaks.xls INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:02:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.10_summits.bed INFO @ Tue, 14 Jul 2020 07:02:30: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (470 records, 4 fields): 12 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Tue, 14 Jul 2020 07:02:32: 9000000 INFO @ Tue, 14 Jul 2020 07:02:32: #1 tag size is determined as 50 bps INFO @ Tue, 14 Jul 2020 07:02:32: #1 tag size = 50 INFO @ Tue, 14 Jul 2020 07:02:32: #1 total tags in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:02:32: #1 user defined the maximum tags... INFO @ Tue, 14 Jul 2020 07:02:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 14 Jul 2020 07:02:32: #1 tags after filtering in treatment: 9031013 INFO @ Tue, 14 Jul 2020 07:02:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 14 Jul 2020 07:02:32: #1 finished! INFO @ Tue, 14 Jul 2020 07:02:32: #2 Build Peak Model... INFO @ Tue, 14 Jul 2020 07:02:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 14 Jul 2020 07:02:33: #2 number of paired peaks: 402 WARNING @ Tue, 14 Jul 2020 07:02:33: Fewer paired peaks (402) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 402 pairs to build model! INFO @ Tue, 14 Jul 2020 07:02:33: start model_add_line... INFO @ Tue, 14 Jul 2020 07:02:33: start X-correlation... INFO @ Tue, 14 Jul 2020 07:02:33: end of X-cor INFO @ Tue, 14 Jul 2020 07:02:33: #2 finished! INFO @ Tue, 14 Jul 2020 07:02:33: #2 predicted fragment length is 46 bps INFO @ Tue, 14 Jul 2020 07:02:33: #2 alternative fragment length(s) may be 2,46,541 bps INFO @ Tue, 14 Jul 2020 07:02:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20_model.r WARNING @ Tue, 14 Jul 2020 07:02:33: #2 Since the d (46) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 14 Jul 2020 07:02:33: #2 You may need to consider one of the other alternative d(s): 2,46,541 WARNING @ Tue, 14 Jul 2020 07:02:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 14 Jul 2020 07:02:33: #3 Call peaks... INFO @ Tue, 14 Jul 2020 07:02:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 14 Jul 2020 07:02:51: #3 Call peaks for each chromosome... INFO @ Tue, 14 Jul 2020 07:03:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20_peaks.xls INFO @ Tue, 14 Jul 2020 07:03:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20_peaks.narrowPeak INFO @ Tue, 14 Jul 2020 07:03:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX7262193/SRX7262193.20_summits.bed INFO @ Tue, 14 Jul 2020 07:03:00: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (177 records, 4 fields): 20 millis CompletedMACS2peakCalling