Job ID = 14157917 SRX = SRX6717209 Genome = ce10 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 13059725 spots for SRR9969881/SRR9969881.sra Written 13059725 spots for SRR9969881/SRR9969881.sra fastq に変換しました。 bowtie でマッピング中... Your job 14158118 ("srTce11") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:52 13059725 reads; of these: 13059725 (100.00%) were unpaired; of these: 12127651 (92.86%) aligned 0 times 802860 (6.15%) aligned exactly 1 time 129214 (0.99%) aligned >1 times 7.14% overall alignment rate Time searching: 00:01:52 Overall time: 00:01:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 211268 / 932074 = 0.2267 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:46:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:46:21: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:46:21: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:46:28: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:46:28: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:46:28: #1 total tags in treatment: 720806 INFO @ Wed, 08 Dec 2021 12:46:28: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:46:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:46:28: #1 tags after filtering in treatment: 720805 INFO @ Wed, 08 Dec 2021 12:46:28: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:46:28: #1 finished! INFO @ Wed, 08 Dec 2021 12:46:28: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:46:28: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:46:28: #2 number of paired peaks: 3043 INFO @ Wed, 08 Dec 2021 12:46:28: start model_add_line... INFO @ Wed, 08 Dec 2021 12:46:28: start X-correlation... INFO @ Wed, 08 Dec 2021 12:46:28: end of X-cor INFO @ Wed, 08 Dec 2021 12:46:28: #2 finished! INFO @ Wed, 08 Dec 2021 12:46:28: #2 predicted fragment length is 117 bps INFO @ Wed, 08 Dec 2021 12:46:28: #2 alternative fragment length(s) may be 117 bps INFO @ Wed, 08 Dec 2021 12:46:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05_model.r INFO @ Wed, 08 Dec 2021 12:46:28: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:46:28: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:46:30: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:46:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05_peaks.xls INFO @ Wed, 08 Dec 2021 12:46:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:46:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.05_summits.bed INFO @ Wed, 08 Dec 2021 12:46:32: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (3427 records, 4 fields): 6 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:46:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:46:51: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:46:51: #1 read treatment tags... INFO @ Wed, 08 Dec 2021 12:46:56: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:46:56: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:46:56: #1 total tags in treatment: 720806 INFO @ Wed, 08 Dec 2021 12:46:56: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:46:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:46:56: #1 tags after filtering in treatment: 720805 INFO @ Wed, 08 Dec 2021 12:46:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:46:56: #1 finished! INFO @ Wed, 08 Dec 2021 12:46:56: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:46:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:46:57: #2 number of paired peaks: 3043 INFO @ Wed, 08 Dec 2021 12:46:57: start model_add_line... INFO @ Wed, 08 Dec 2021 12:46:57: start X-correlation... INFO @ Wed, 08 Dec 2021 12:46:57: end of X-cor INFO @ Wed, 08 Dec 2021 12:46:57: #2 finished! INFO @ Wed, 08 Dec 2021 12:46:57: #2 predicted fragment length is 117 bps INFO @ Wed, 08 Dec 2021 12:46:57: #2 alternative fragment length(s) may be 117 bps INFO @ Wed, 08 Dec 2021 12:46:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10_model.r INFO @ Wed, 08 Dec 2021 12:46:57: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:46:57: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:46:59: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:47:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10_peaks.xls INFO @ Wed, 08 Dec 2021 12:47:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:47:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.10_summits.bed INFO @ Wed, 08 Dec 2021 12:47:00: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (1522 records, 4 fields): 3 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 08 Dec 2021 12:47:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 08 Dec 2021 12:47:22: #1 read tag files... INFO @ Wed, 08 Dec 2021 12:47:22: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Wed, 08 Dec 2021 12:47:27: #1 tag size is determined as 51 bps INFO @ Wed, 08 Dec 2021 12:47:27: #1 tag size = 51 INFO @ Wed, 08 Dec 2021 12:47:27: #1 total tags in treatment: 720806 INFO @ Wed, 08 Dec 2021 12:47:27: #1 user defined the maximum tags... INFO @ Wed, 08 Dec 2021 12:47:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 08 Dec 2021 12:47:27: #1 tags after filtering in treatment: 720805 INFO @ Wed, 08 Dec 2021 12:47:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 08 Dec 2021 12:47:27: #1 finished! INFO @ Wed, 08 Dec 2021 12:47:27: #2 Build Peak Model... INFO @ Wed, 08 Dec 2021 12:47:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 08 Dec 2021 12:47:27: #2 number of paired peaks: 3043 INFO @ Wed, 08 Dec 2021 12:47:27: start model_add_line... INFO @ Wed, 08 Dec 2021 12:47:27: start X-correlation... INFO @ Wed, 08 Dec 2021 12:47:27: end of X-cor INFO @ Wed, 08 Dec 2021 12:47:27: #2 finished! INFO @ Wed, 08 Dec 2021 12:47:27: #2 predicted fragment length is 117 bps INFO @ Wed, 08 Dec 2021 12:47:27: #2 alternative fragment length(s) may be 117 bps INFO @ Wed, 08 Dec 2021 12:47:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20_model.r INFO @ Wed, 08 Dec 2021 12:47:27: #3 Call peaks... INFO @ Wed, 08 Dec 2021 12:47:27: #3 Pre-compute pvalue-qvalue table... INFO @ Wed, 08 Dec 2021 12:47:30: #3 Call peaks for each chromosome... INFO @ Wed, 08 Dec 2021 12:47:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20_peaks.xls INFO @ Wed, 08 Dec 2021 12:47:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20_peaks.narrowPeak INFO @ Wed, 08 Dec 2021 12:47:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6717209/SRX6717209.20_summits.bed INFO @ Wed, 08 Dec 2021 12:47:31: Done! pass1 - making usageList (6 chroms): 2 millis pass2 - checking and writing primary data (536 records, 4 fields): 2 millis CompletedMACS2peakCalling