Job ID = 5720214 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:37:13 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:44:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:45:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:50:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:19 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:05:25 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:33:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:36:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:39:55 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:45:43 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 50,927,183 reads read : 101,854,366 reads written : 101,854,366 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:48:10 50927183 reads; of these: 50927183 (100.00%) were paired; of these: 16656247 (32.71%) aligned concordantly 0 times 28741131 (56.44%) aligned concordantly exactly 1 time 5529805 (10.86%) aligned concordantly >1 times ---- 16656247 pairs aligned concordantly 0 times; of these: 10698964 (64.23%) aligned discordantly 1 time ---- 5957283 pairs aligned 0 times concordantly or discordantly; of these: 11914566 mates make up the pairs; of these: 6947003 (58.31%) aligned 0 times 1909529 (16.03%) aligned exactly 1 time 3058034 (25.67%) aligned >1 times 93.18% overall alignment rate Time searching: 01:48:10 Overall time: 01:48:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 72 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 7379000 / 44659861 = 0.1652 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:12:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:12:54: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:12:54: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:13:01: 1000000 INFO @ Thu, 16 Apr 2020 01:13:09: 2000000 INFO @ Thu, 16 Apr 2020 01:13:16: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:13:23: 4000000 INFO @ Thu, 16 Apr 2020 01:13:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:13:24: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:13:24: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:13:32: 5000000 INFO @ Thu, 16 Apr 2020 01:13:34: 1000000 INFO @ Thu, 16 Apr 2020 01:13:40: 6000000 INFO @ Thu, 16 Apr 2020 01:13:44: 2000000 INFO @ Thu, 16 Apr 2020 01:13:49: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 01:13:54: 3000000 INFO @ Thu, 16 Apr 2020 01:13:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 01:13:54: #1 read tag files... INFO @ Thu, 16 Apr 2020 01:13:54: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 01:13:57: 8000000 INFO @ Thu, 16 Apr 2020 01:14:03: 1000000 INFO @ Thu, 16 Apr 2020 01:14:04: 4000000 INFO @ Thu, 16 Apr 2020 01:14:06: 9000000 INFO @ Thu, 16 Apr 2020 01:14:12: 2000000 INFO @ Thu, 16 Apr 2020 01:14:15: 5000000 INFO @ Thu, 16 Apr 2020 01:14:16: 10000000 INFO @ Thu, 16 Apr 2020 01:14:21: 3000000 INFO @ Thu, 16 Apr 2020 01:14:25: 11000000 INFO @ Thu, 16 Apr 2020 01:14:25: 6000000 INFO @ Thu, 16 Apr 2020 01:14:30: 4000000 INFO @ Thu, 16 Apr 2020 01:14:34: 12000000 INFO @ Thu, 16 Apr 2020 01:14:35: 7000000 INFO @ Thu, 16 Apr 2020 01:14:39: 5000000 INFO @ Thu, 16 Apr 2020 01:14:43: 13000000 INFO @ Thu, 16 Apr 2020 01:14:45: 8000000 INFO @ Thu, 16 Apr 2020 01:14:48: 6000000 INFO @ Thu, 16 Apr 2020 01:14:52: 14000000 INFO @ Thu, 16 Apr 2020 01:14:55: 9000000 INFO @ Thu, 16 Apr 2020 01:14:57: 7000000 INFO @ Thu, 16 Apr 2020 01:15:01: 15000000 INFO @ Thu, 16 Apr 2020 01:15:06: 10000000 INFO @ Thu, 16 Apr 2020 01:15:06: 8000000 INFO @ Thu, 16 Apr 2020 01:15:10: 16000000 INFO @ Thu, 16 Apr 2020 01:15:15: 9000000 INFO @ Thu, 16 Apr 2020 01:15:16: 11000000 INFO @ Thu, 16 Apr 2020 01:15:19: 17000000 INFO @ Thu, 16 Apr 2020 01:15:25: 10000000 INFO @ Thu, 16 Apr 2020 01:15:26: 12000000 INFO @ Thu, 16 Apr 2020 01:15:28: 18000000 INFO @ Thu, 16 Apr 2020 01:15:34: 11000000 INFO @ Thu, 16 Apr 2020 01:15:37: 13000000 INFO @ Thu, 16 Apr 2020 01:15:37: 19000000 INFO @ Thu, 16 Apr 2020 01:15:43: 12000000 INFO @ Thu, 16 Apr 2020 01:15:46: 20000000 INFO @ Thu, 16 Apr 2020 01:15:47: 14000000 INFO @ Thu, 16 Apr 2020 01:15:52: 13000000 INFO @ Thu, 16 Apr 2020 01:15:55: 21000000 INFO @ Thu, 16 Apr 2020 01:15:57: 15000000 INFO @ Thu, 16 Apr 2020 01:16:00: 14000000 INFO @ Thu, 16 Apr 2020 01:16:04: 22000000 INFO @ Thu, 16 Apr 2020 01:16:08: 16000000 INFO @ Thu, 16 Apr 2020 01:16:09: 15000000 INFO @ Thu, 16 Apr 2020 01:16:13: 23000000 INFO @ Thu, 16 Apr 2020 01:16:18: 17000000 INFO @ Thu, 16 Apr 2020 01:16:19: 16000000 INFO @ Thu, 16 Apr 2020 01:16:22: 24000000 INFO @ Thu, 16 Apr 2020 01:16:28: 17000000 INFO @ Thu, 16 Apr 2020 01:16:28: 18000000 INFO @ Thu, 16 Apr 2020 01:16:31: 25000000 INFO @ Thu, 16 Apr 2020 01:16:37: 18000000 INFO @ Thu, 16 Apr 2020 01:16:38: 19000000 INFO @ Thu, 16 Apr 2020 01:16:40: 26000000 INFO @ Thu, 16 Apr 2020 01:16:46: 19000000 INFO @ Thu, 16 Apr 2020 01:16:48: 20000000 INFO @ Thu, 16 Apr 2020 01:16:49: 27000000 INFO @ Thu, 16 Apr 2020 01:16:54: 20000000 INFO @ Thu, 16 Apr 2020 01:16:57: 21000000 INFO @ Thu, 16 Apr 2020 01:16:58: 28000000 INFO @ Thu, 16 Apr 2020 01:17:03: 21000000 INFO @ Thu, 16 Apr 2020 01:17:07: 29000000 INFO @ Thu, 16 Apr 2020 01:17:07: 22000000 INFO @ Thu, 16 Apr 2020 01:17:12: 22000000 INFO @ Thu, 16 Apr 2020 01:17:16: 30000000 INFO @ Thu, 16 Apr 2020 01:17:17: 23000000 INFO @ Thu, 16 Apr 2020 01:17:21: 23000000 INFO @ Thu, 16 Apr 2020 01:17:25: 31000000 INFO @ Thu, 16 Apr 2020 01:17:27: 24000000 INFO @ Thu, 16 Apr 2020 01:17:30: 24000000 INFO @ Thu, 16 Apr 2020 01:17:34: 32000000 INFO @ Thu, 16 Apr 2020 01:17:37: 25000000 INFO @ Thu, 16 Apr 2020 01:17:39: 25000000 INFO @ Thu, 16 Apr 2020 01:17:43: 33000000 INFO @ Thu, 16 Apr 2020 01:17:47: 26000000 INFO @ Thu, 16 Apr 2020 01:17:48: 26000000 INFO @ Thu, 16 Apr 2020 01:17:52: 34000000 INFO @ Thu, 16 Apr 2020 01:17:57: 27000000 INFO @ Thu, 16 Apr 2020 01:17:58: 27000000 INFO @ Thu, 16 Apr 2020 01:18:01: 35000000 INFO @ Thu, 16 Apr 2020 01:18:07: 28000000 INFO @ Thu, 16 Apr 2020 01:18:08: 28000000 INFO @ Thu, 16 Apr 2020 01:18:10: 36000000 INFO @ Thu, 16 Apr 2020 01:18:17: 29000000 INFO @ Thu, 16 Apr 2020 01:18:18: 29000000 INFO @ Thu, 16 Apr 2020 01:18:18: 37000000 INFO @ Thu, 16 Apr 2020 01:18:27: 38000000 INFO @ Thu, 16 Apr 2020 01:18:27: 30000000 INFO @ Thu, 16 Apr 2020 01:18:28: 30000000 INFO @ Thu, 16 Apr 2020 01:18:36: 39000000 INFO @ Thu, 16 Apr 2020 01:18:38: 31000000 INFO @ Thu, 16 Apr 2020 01:18:39: 31000000 INFO @ Thu, 16 Apr 2020 01:18:44: 40000000 INFO @ Thu, 16 Apr 2020 01:18:48: 32000000 INFO @ Thu, 16 Apr 2020 01:18:49: 32000000 INFO @ Thu, 16 Apr 2020 01:18:52: 41000000 INFO @ Thu, 16 Apr 2020 01:18:58: 33000000 INFO @ Thu, 16 Apr 2020 01:18:59: 33000000 INFO @ Thu, 16 Apr 2020 01:19:01: 42000000 INFO @ Thu, 16 Apr 2020 01:19:08: 34000000 INFO @ Thu, 16 Apr 2020 01:19:09: 34000000 INFO @ Thu, 16 Apr 2020 01:19:09: 43000000 INFO @ Thu, 16 Apr 2020 01:19:18: 44000000 INFO @ Thu, 16 Apr 2020 01:19:18: 35000000 INFO @ Thu, 16 Apr 2020 01:19:19: 35000000 INFO @ Thu, 16 Apr 2020 01:19:27: 45000000 INFO @ Thu, 16 Apr 2020 01:19:28: 36000000 INFO @ Thu, 16 Apr 2020 01:19:29: 36000000 INFO @ Thu, 16 Apr 2020 01:19:35: 46000000 INFO @ Thu, 16 Apr 2020 01:19:39: 37000000 INFO @ Thu, 16 Apr 2020 01:19:40: 37000000 INFO @ Thu, 16 Apr 2020 01:19:44: 47000000 INFO @ Thu, 16 Apr 2020 01:19:49: 38000000 INFO @ Thu, 16 Apr 2020 01:19:50: 38000000 INFO @ Thu, 16 Apr 2020 01:19:53: 48000000 INFO @ Thu, 16 Apr 2020 01:19:59: 39000000 INFO @ Thu, 16 Apr 2020 01:20:00: 39000000 INFO @ Thu, 16 Apr 2020 01:20:02: 49000000 INFO @ Thu, 16 Apr 2020 01:20:09: 40000000 INFO @ Thu, 16 Apr 2020 01:20:10: 40000000 INFO @ Thu, 16 Apr 2020 01:20:11: 50000000 INFO @ Thu, 16 Apr 2020 01:20:19: 41000000 INFO @ Thu, 16 Apr 2020 01:20:20: 51000000 INFO @ Thu, 16 Apr 2020 01:20:20: 41000000 INFO @ Thu, 16 Apr 2020 01:20:28: 52000000 INFO @ Thu, 16 Apr 2020 01:20:29: 42000000 INFO @ Thu, 16 Apr 2020 01:20:30: 42000000 INFO @ Thu, 16 Apr 2020 01:20:37: 53000000 INFO @ Thu, 16 Apr 2020 01:20:39: 43000000 INFO @ Thu, 16 Apr 2020 01:20:40: 43000000 INFO @ Thu, 16 Apr 2020 01:20:46: 54000000 INFO @ Thu, 16 Apr 2020 01:20:49: 44000000 INFO @ Thu, 16 Apr 2020 01:20:50: 44000000 INFO @ Thu, 16 Apr 2020 01:20:55: 55000000 INFO @ Thu, 16 Apr 2020 01:20:59: 45000000 INFO @ Thu, 16 Apr 2020 01:21:00: 45000000 INFO @ Thu, 16 Apr 2020 01:21:03: 56000000 INFO @ Thu, 16 Apr 2020 01:21:09: 46000000 INFO @ Thu, 16 Apr 2020 01:21:10: 46000000 INFO @ Thu, 16 Apr 2020 01:21:12: 57000000 INFO @ Thu, 16 Apr 2020 01:21:19: 47000000 INFO @ Thu, 16 Apr 2020 01:21:19: 47000000 INFO @ Thu, 16 Apr 2020 01:21:20: 58000000 INFO @ Thu, 16 Apr 2020 01:21:29: 48000000 INFO @ Thu, 16 Apr 2020 01:21:29: 59000000 INFO @ Thu, 16 Apr 2020 01:21:29: 48000000 INFO @ Thu, 16 Apr 2020 01:21:37: 60000000 INFO @ Thu, 16 Apr 2020 01:21:38: 49000000 INFO @ Thu, 16 Apr 2020 01:21:39: 49000000 INFO @ Thu, 16 Apr 2020 01:21:46: 61000000 INFO @ Thu, 16 Apr 2020 01:21:48: 50000000 INFO @ Thu, 16 Apr 2020 01:21:49: 50000000 INFO @ Thu, 16 Apr 2020 01:21:54: 62000000 INFO @ Thu, 16 Apr 2020 01:21:58: 51000000 INFO @ Thu, 16 Apr 2020 01:21:59: 51000000 INFO @ Thu, 16 Apr 2020 01:22:03: 63000000 INFO @ Thu, 16 Apr 2020 01:22:08: 52000000 INFO @ Thu, 16 Apr 2020 01:22:09: 52000000 INFO @ Thu, 16 Apr 2020 01:22:11: 64000000 INFO @ Thu, 16 Apr 2020 01:22:18: 53000000 INFO @ Thu, 16 Apr 2020 01:22:18: 53000000 INFO @ Thu, 16 Apr 2020 01:22:19: 65000000 INFO @ Thu, 16 Apr 2020 01:22:27: 54000000 INFO @ Thu, 16 Apr 2020 01:22:28: 66000000 INFO @ Thu, 16 Apr 2020 01:22:28: 54000000 INFO @ Thu, 16 Apr 2020 01:22:37: 67000000 INFO @ Thu, 16 Apr 2020 01:22:37: 55000000 INFO @ Thu, 16 Apr 2020 01:22:38: 55000000 INFO @ Thu, 16 Apr 2020 01:22:46: 68000000 INFO @ Thu, 16 Apr 2020 01:22:47: 56000000 INFO @ Thu, 16 Apr 2020 01:22:48: 56000000 INFO @ Thu, 16 Apr 2020 01:22:54: 69000000 INFO @ Thu, 16 Apr 2020 01:22:57: 57000000 INFO @ Thu, 16 Apr 2020 01:22:58: 57000000 INFO @ Thu, 16 Apr 2020 01:23:03: 70000000 INFO @ Thu, 16 Apr 2020 01:23:07: 58000000 INFO @ Thu, 16 Apr 2020 01:23:08: 58000000 INFO @ Thu, 16 Apr 2020 01:23:11: 71000000 INFO @ Thu, 16 Apr 2020 01:23:17: 59000000 INFO @ Thu, 16 Apr 2020 01:23:18: 59000000 INFO @ Thu, 16 Apr 2020 01:23:20: 72000000 INFO @ Thu, 16 Apr 2020 01:23:27: 60000000 INFO @ Thu, 16 Apr 2020 01:23:27: 60000000 INFO @ Thu, 16 Apr 2020 01:23:28: 73000000 INFO @ Thu, 16 Apr 2020 01:23:36: 74000000 INFO @ Thu, 16 Apr 2020 01:23:36: 61000000 INFO @ Thu, 16 Apr 2020 01:23:37: 61000000 INFO @ Thu, 16 Apr 2020 01:23:45: 75000000 INFO @ Thu, 16 Apr 2020 01:23:46: 62000000 INFO @ Thu, 16 Apr 2020 01:23:47: 62000000 INFO @ Thu, 16 Apr 2020 01:23:53: 76000000 INFO @ Thu, 16 Apr 2020 01:23:56: 63000000 INFO @ Thu, 16 Apr 2020 01:23:56: 63000000 INFO @ Thu, 16 Apr 2020 01:24:01: 77000000 INFO @ Thu, 16 Apr 2020 01:24:06: 64000000 INFO @ Thu, 16 Apr 2020 01:24:06: 64000000 INFO @ Thu, 16 Apr 2020 01:24:09: 78000000 INFO @ Thu, 16 Apr 2020 01:24:15: 65000000 INFO @ Thu, 16 Apr 2020 01:24:16: 65000000 INFO @ Thu, 16 Apr 2020 01:24:18: 79000000 INFO @ Thu, 16 Apr 2020 01:24:25: 66000000 INFO @ Thu, 16 Apr 2020 01:24:26: 66000000 INFO @ Thu, 16 Apr 2020 01:24:26: 80000000 INFO @ Thu, 16 Apr 2020 01:24:28: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:24:28: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:24:28: #1 total tags in treatment: 28570144 INFO @ Thu, 16 Apr 2020 01:24:28: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:24:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:24:28: #1 tags after filtering in treatment: 22007668 INFO @ Thu, 16 Apr 2020 01:24:28: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 01:24:28: #1 finished! INFO @ Thu, 16 Apr 2020 01:24:28: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:24:28: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:24:30: #2 number of paired peaks: 236 WARNING @ Thu, 16 Apr 2020 01:24:30: Fewer paired peaks (236) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 236 pairs to build model! INFO @ Thu, 16 Apr 2020 01:24:30: start model_add_line... INFO @ Thu, 16 Apr 2020 01:24:30: start X-correlation... INFO @ Thu, 16 Apr 2020 01:24:30: end of X-cor INFO @ Thu, 16 Apr 2020 01:24:30: #2 finished! INFO @ Thu, 16 Apr 2020 01:24:30: #2 predicted fragment length is 215 bps INFO @ Thu, 16 Apr 2020 01:24:30: #2 alternative fragment length(s) may be 3,201,215 bps INFO @ Thu, 16 Apr 2020 01:24:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05_model.r WARNING @ Thu, 16 Apr 2020 01:24:30: #2 Since the d (215) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:24:30: #2 You may need to consider one of the other alternative d(s): 3,201,215 WARNING @ Thu, 16 Apr 2020 01:24:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:24:30: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:24:30: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:24:35: 67000000 INFO @ Thu, 16 Apr 2020 01:24:36: 67000000 INFO @ Thu, 16 Apr 2020 01:24:45: 68000000 INFO @ Thu, 16 Apr 2020 01:24:45: 68000000 INFO @ Thu, 16 Apr 2020 01:24:54: 69000000 INFO @ Thu, 16 Apr 2020 01:24:55: 69000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 01:25:04: 70000000 INFO @ Thu, 16 Apr 2020 01:25:05: 70000000 INFO @ Thu, 16 Apr 2020 01:25:14: 71000000 INFO @ Thu, 16 Apr 2020 01:25:14: 71000000 INFO @ Thu, 16 Apr 2020 01:25:16: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:25:23: 72000000 INFO @ Thu, 16 Apr 2020 01:25:24: 72000000 INFO @ Thu, 16 Apr 2020 01:25:33: 73000000 INFO @ Thu, 16 Apr 2020 01:25:33: 73000000 INFO @ Thu, 16 Apr 2020 01:25:38: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05_peaks.xls INFO @ Thu, 16 Apr 2020 01:25:39: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:25:39: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.05_summits.bed INFO @ Thu, 16 Apr 2020 01:25:39: Done! pass1 - making usageList (7 chroms): 4 millis pass2 - checking and writing primary data (34002 records, 4 fields): 29 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:25:42: 74000000 INFO @ Thu, 16 Apr 2020 01:25:43: 74000000 INFO @ Thu, 16 Apr 2020 01:25:52: 75000000 INFO @ Thu, 16 Apr 2020 01:25:52: 75000000 INFO @ Thu, 16 Apr 2020 01:26:01: 76000000 INFO @ Thu, 16 Apr 2020 01:26:02: 76000000 INFO @ Thu, 16 Apr 2020 01:26:11: 77000000 INFO @ Thu, 16 Apr 2020 01:26:11: 77000000 BigWig に変換しました。 INFO @ Thu, 16 Apr 2020 01:26:20: 78000000 INFO @ Thu, 16 Apr 2020 01:26:21: 78000000 INFO @ Thu, 16 Apr 2020 01:26:30: 79000000 INFO @ Thu, 16 Apr 2020 01:26:31: 79000000 INFO @ Thu, 16 Apr 2020 01:26:40: 80000000 INFO @ Thu, 16 Apr 2020 01:26:41: 80000000 INFO @ Thu, 16 Apr 2020 01:26:42: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:26:42: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:26:42: #1 total tags in treatment: 28570144 INFO @ Thu, 16 Apr 2020 01:26:42: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:26:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:26:42: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 01:26:42: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 01:26:42: #1 total tags in treatment: 28570144 INFO @ Thu, 16 Apr 2020 01:26:42: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 01:26:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 01:26:42: #1 tags after filtering in treatment: 22007668 INFO @ Thu, 16 Apr 2020 01:26:42: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 01:26:42: #1 finished! INFO @ Thu, 16 Apr 2020 01:26:42: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:26:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:26:43: #1 tags after filtering in treatment: 22007668 INFO @ Thu, 16 Apr 2020 01:26:43: #1 Redundant rate of treatment: 0.23 INFO @ Thu, 16 Apr 2020 01:26:43: #1 finished! INFO @ Thu, 16 Apr 2020 01:26:43: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 01:26:43: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 01:26:44: #2 number of paired peaks: 236 WARNING @ Thu, 16 Apr 2020 01:26:44: Fewer paired peaks (236) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 236 pairs to build model! INFO @ Thu, 16 Apr 2020 01:26:44: start model_add_line... INFO @ Thu, 16 Apr 2020 01:26:44: start X-correlation... INFO @ Thu, 16 Apr 2020 01:26:44: end of X-cor INFO @ Thu, 16 Apr 2020 01:26:44: #2 finished! INFO @ Thu, 16 Apr 2020 01:26:44: #2 predicted fragment length is 215 bps INFO @ Thu, 16 Apr 2020 01:26:44: #2 alternative fragment length(s) may be 3,201,215 bps INFO @ Thu, 16 Apr 2020 01:26:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10_model.r WARNING @ Thu, 16 Apr 2020 01:26:44: #2 Since the d (215) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:26:44: #2 You may need to consider one of the other alternative d(s): 3,201,215 WARNING @ Thu, 16 Apr 2020 01:26:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:26:44: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:26:44: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:26:44: #2 number of paired peaks: 236 WARNING @ Thu, 16 Apr 2020 01:26:44: Fewer paired peaks (236) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 236 pairs to build model! INFO @ Thu, 16 Apr 2020 01:26:44: start model_add_line... INFO @ Thu, 16 Apr 2020 01:26:44: start X-correlation... INFO @ Thu, 16 Apr 2020 01:26:44: end of X-cor INFO @ Thu, 16 Apr 2020 01:26:44: #2 finished! INFO @ Thu, 16 Apr 2020 01:26:44: #2 predicted fragment length is 215 bps INFO @ Thu, 16 Apr 2020 01:26:44: #2 alternative fragment length(s) may be 3,201,215 bps INFO @ Thu, 16 Apr 2020 01:26:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20_model.r WARNING @ Thu, 16 Apr 2020 01:26:44: #2 Since the d (215) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 01:26:44: #2 You may need to consider one of the other alternative d(s): 3,201,215 WARNING @ Thu, 16 Apr 2020 01:26:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 01:26:44: #3 Call peaks... INFO @ Thu, 16 Apr 2020 01:26:44: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 01:27:29: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:27:29: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20_peaks.xls INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.20_summits.bed INFO @ Thu, 16 Apr 2020 01:27:50: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (6551 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10_peaks.xls INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 01:27:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619587/SRX6619587.10_summits.bed INFO @ Thu, 16 Apr 2020 01:27:51: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (20163 records, 4 fields): 23 millis CompletedMACS2peakCalling