Job ID = 5720211 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T13:00:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:15:39 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:27:02 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:28:08 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:35:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:36:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:39:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:43:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 43,555,027 reads read : 87,110,054 reads written : 87,110,054 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:54 43555027 reads; of these: 43555027 (100.00%) were paired; of these: 27937589 (64.14%) aligned concordantly 0 times 12830737 (29.46%) aligned concordantly exactly 1 time 2786701 (6.40%) aligned concordantly >1 times ---- 27937589 pairs aligned concordantly 0 times; of these: 14023312 (50.20%) aligned discordantly 1 time ---- 13914277 pairs aligned 0 times concordantly or discordantly; of these: 27828554 mates make up the pairs; of these: 24164934 (86.84%) aligned 0 times 1279022 (4.60%) aligned exactly 1 time 2384598 (8.57%) aligned >1 times 72.26% overall alignment rate Time searching: 01:09:54 Overall time: 01:09:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4682143 / 29063535 = 0.1611 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:40:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:40:05: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:40:05: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:40:13: 1000000 INFO @ Thu, 16 Apr 2020 00:40:20: 2000000 INFO @ Thu, 16 Apr 2020 00:40:28: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:40:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:40:35: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:40:35: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:40:35: 4000000 INFO @ Thu, 16 Apr 2020 00:40:45: 5000000 INFO @ Thu, 16 Apr 2020 00:40:46: 1000000 INFO @ Thu, 16 Apr 2020 00:40:55: 6000000 INFO @ Thu, 16 Apr 2020 00:40:57: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:41:05: 7000000 INFO @ Thu, 16 Apr 2020 00:41:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:41:06: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:41:06: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:41:08: 3000000 INFO @ Thu, 16 Apr 2020 00:41:15: 8000000 INFO @ Thu, 16 Apr 2020 00:41:16: 1000000 INFO @ Thu, 16 Apr 2020 00:41:19: 4000000 INFO @ Thu, 16 Apr 2020 00:41:25: 9000000 INFO @ Thu, 16 Apr 2020 00:41:26: 2000000 INFO @ Thu, 16 Apr 2020 00:41:30: 5000000 INFO @ Thu, 16 Apr 2020 00:41:35: 10000000 INFO @ Thu, 16 Apr 2020 00:41:36: 3000000 INFO @ Thu, 16 Apr 2020 00:41:40: 6000000 INFO @ Thu, 16 Apr 2020 00:41:45: 11000000 INFO @ Thu, 16 Apr 2020 00:41:46: 4000000 INFO @ Thu, 16 Apr 2020 00:41:52: 7000000 INFO @ Thu, 16 Apr 2020 00:41:55: 12000000 INFO @ Thu, 16 Apr 2020 00:41:56: 5000000 INFO @ Thu, 16 Apr 2020 00:42:04: 8000000 INFO @ Thu, 16 Apr 2020 00:42:05: 13000000 INFO @ Thu, 16 Apr 2020 00:42:06: 6000000 INFO @ Thu, 16 Apr 2020 00:42:15: 14000000 INFO @ Thu, 16 Apr 2020 00:42:15: 9000000 INFO @ Thu, 16 Apr 2020 00:42:16: 7000000 INFO @ Thu, 16 Apr 2020 00:42:25: 15000000 INFO @ Thu, 16 Apr 2020 00:42:26: 8000000 INFO @ Thu, 16 Apr 2020 00:42:27: 10000000 INFO @ Thu, 16 Apr 2020 00:42:35: 16000000 INFO @ Thu, 16 Apr 2020 00:42:36: 9000000 INFO @ Thu, 16 Apr 2020 00:42:38: 11000000 INFO @ Thu, 16 Apr 2020 00:42:45: 17000000 INFO @ Thu, 16 Apr 2020 00:42:46: 10000000 INFO @ Thu, 16 Apr 2020 00:42:50: 12000000 INFO @ Thu, 16 Apr 2020 00:42:55: 18000000 INFO @ Thu, 16 Apr 2020 00:42:56: 11000000 INFO @ Thu, 16 Apr 2020 00:43:01: 13000000 INFO @ Thu, 16 Apr 2020 00:43:05: 19000000 INFO @ Thu, 16 Apr 2020 00:43:06: 12000000 INFO @ Thu, 16 Apr 2020 00:43:12: 14000000 INFO @ Thu, 16 Apr 2020 00:43:15: 20000000 INFO @ Thu, 16 Apr 2020 00:43:16: 13000000 INFO @ Thu, 16 Apr 2020 00:43:23: 15000000 INFO @ Thu, 16 Apr 2020 00:43:25: 21000000 INFO @ Thu, 16 Apr 2020 00:43:26: 14000000 INFO @ Thu, 16 Apr 2020 00:43:34: 16000000 INFO @ Thu, 16 Apr 2020 00:43:35: 22000000 INFO @ Thu, 16 Apr 2020 00:43:36: 15000000 INFO @ Thu, 16 Apr 2020 00:43:45: 17000000 INFO @ Thu, 16 Apr 2020 00:43:45: 23000000 INFO @ Thu, 16 Apr 2020 00:43:46: 16000000 INFO @ Thu, 16 Apr 2020 00:43:55: 24000000 INFO @ Thu, 16 Apr 2020 00:43:56: 18000000 INFO @ Thu, 16 Apr 2020 00:43:56: 17000000 INFO @ Thu, 16 Apr 2020 00:44:05: 25000000 INFO @ Thu, 16 Apr 2020 00:44:06: 18000000 INFO @ Thu, 16 Apr 2020 00:44:07: 19000000 INFO @ Thu, 16 Apr 2020 00:44:15: 26000000 INFO @ Thu, 16 Apr 2020 00:44:16: 19000000 INFO @ Thu, 16 Apr 2020 00:44:18: 20000000 INFO @ Thu, 16 Apr 2020 00:44:26: 27000000 INFO @ Thu, 16 Apr 2020 00:44:26: 20000000 INFO @ Thu, 16 Apr 2020 00:44:29: 21000000 INFO @ Thu, 16 Apr 2020 00:44:36: 28000000 INFO @ Thu, 16 Apr 2020 00:44:36: 21000000 INFO @ Thu, 16 Apr 2020 00:44:40: 22000000 INFO @ Thu, 16 Apr 2020 00:44:46: 29000000 INFO @ Thu, 16 Apr 2020 00:44:46: 22000000 INFO @ Thu, 16 Apr 2020 00:44:51: 23000000 INFO @ Thu, 16 Apr 2020 00:44:56: 30000000 INFO @ Thu, 16 Apr 2020 00:44:56: 23000000 INFO @ Thu, 16 Apr 2020 00:45:02: 24000000 INFO @ Thu, 16 Apr 2020 00:45:06: 31000000 INFO @ Thu, 16 Apr 2020 00:45:07: 24000000 INFO @ Thu, 16 Apr 2020 00:45:13: 25000000 INFO @ Thu, 16 Apr 2020 00:45:16: 32000000 INFO @ Thu, 16 Apr 2020 00:45:17: 25000000 INFO @ Thu, 16 Apr 2020 00:45:25: 26000000 INFO @ Thu, 16 Apr 2020 00:45:26: 33000000 INFO @ Thu, 16 Apr 2020 00:45:27: 26000000 INFO @ Thu, 16 Apr 2020 00:45:36: 27000000 INFO @ Thu, 16 Apr 2020 00:45:36: 34000000 INFO @ Thu, 16 Apr 2020 00:45:37: 27000000 INFO @ Thu, 16 Apr 2020 00:45:46: 35000000 INFO @ Thu, 16 Apr 2020 00:45:47: 28000000 INFO @ Thu, 16 Apr 2020 00:45:48: 28000000 INFO @ Thu, 16 Apr 2020 00:45:56: 36000000 INFO @ Thu, 16 Apr 2020 00:45:58: 29000000 INFO @ Thu, 16 Apr 2020 00:45:59: 29000000 INFO @ Thu, 16 Apr 2020 00:46:06: 37000000 INFO @ Thu, 16 Apr 2020 00:46:09: 30000000 INFO @ Thu, 16 Apr 2020 00:46:10: 30000000 INFO @ Thu, 16 Apr 2020 00:46:16: 38000000 INFO @ Thu, 16 Apr 2020 00:46:21: 31000000 INFO @ Thu, 16 Apr 2020 00:46:21: 31000000 INFO @ Thu, 16 Apr 2020 00:46:26: 39000000 INFO @ Thu, 16 Apr 2020 00:46:32: 32000000 INFO @ Thu, 16 Apr 2020 00:46:33: 32000000 INFO @ Thu, 16 Apr 2020 00:46:36: 40000000 INFO @ Thu, 16 Apr 2020 00:46:43: 33000000 INFO @ Thu, 16 Apr 2020 00:46:44: 33000000 INFO @ Thu, 16 Apr 2020 00:46:46: 41000000 INFO @ Thu, 16 Apr 2020 00:46:54: 34000000 INFO @ Thu, 16 Apr 2020 00:46:55: 34000000 INFO @ Thu, 16 Apr 2020 00:46:55: 42000000 INFO @ Thu, 16 Apr 2020 00:47:05: 35000000 INFO @ Thu, 16 Apr 2020 00:47:05: 43000000 INFO @ Thu, 16 Apr 2020 00:47:05: 35000000 INFO @ Thu, 16 Apr 2020 00:47:15: 44000000 INFO @ Thu, 16 Apr 2020 00:47:15: 36000000 INFO @ Thu, 16 Apr 2020 00:47:16: 36000000 INFO @ Thu, 16 Apr 2020 00:47:25: 45000000 INFO @ Thu, 16 Apr 2020 00:47:26: 37000000 INFO @ Thu, 16 Apr 2020 00:47:27: 37000000 INFO @ Thu, 16 Apr 2020 00:47:35: 46000000 INFO @ Thu, 16 Apr 2020 00:47:37: 38000000 INFO @ Thu, 16 Apr 2020 00:47:37: 38000000 INFO @ Thu, 16 Apr 2020 00:47:45: 47000000 INFO @ Thu, 16 Apr 2020 00:47:47: 39000000 INFO @ Thu, 16 Apr 2020 00:47:48: 39000000 INFO @ Thu, 16 Apr 2020 00:47:55: 48000000 INFO @ Thu, 16 Apr 2020 00:47:58: 40000000 INFO @ Thu, 16 Apr 2020 00:47:59: 40000000 INFO @ Thu, 16 Apr 2020 00:48:04: 49000000 INFO @ Thu, 16 Apr 2020 00:48:09: 41000000 INFO @ Thu, 16 Apr 2020 00:48:09: 41000000 INFO @ Thu, 16 Apr 2020 00:48:14: 50000000 INFO @ Thu, 16 Apr 2020 00:48:19: 42000000 INFO @ Thu, 16 Apr 2020 00:48:20: 42000000 INFO @ Thu, 16 Apr 2020 00:48:24: 51000000 INFO @ Thu, 16 Apr 2020 00:48:30: 43000000 INFO @ Thu, 16 Apr 2020 00:48:31: 43000000 INFO @ Thu, 16 Apr 2020 00:48:34: 52000000 INFO @ Thu, 16 Apr 2020 00:48:41: 44000000 INFO @ Thu, 16 Apr 2020 00:48:41: 44000000 INFO @ Thu, 16 Apr 2020 00:48:44: 53000000 INFO @ Thu, 16 Apr 2020 00:48:50: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:48:50: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:48:50: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:48:50: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:48:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:48:50: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:48:50: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:48:50: #1 finished! INFO @ Thu, 16 Apr 2020 00:48:50: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:48:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:48:51: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:48:51: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:48:51: start model_add_line... INFO @ Thu, 16 Apr 2020 00:48:51: start X-correlation... INFO @ Thu, 16 Apr 2020 00:48:51: end of X-cor INFO @ Thu, 16 Apr 2020 00:48:51: #2 finished! INFO @ Thu, 16 Apr 2020 00:48:51: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:48:51: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:48:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05_model.r WARNING @ Thu, 16 Apr 2020 00:48:51: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:48:51: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:48:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:48:51: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:48:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:48:51: 45000000 INFO @ Thu, 16 Apr 2020 00:48:52: 45000000 INFO @ Thu, 16 Apr 2020 00:49:02: 46000000 INFO @ Thu, 16 Apr 2020 00:49:03: 46000000 INFO @ Thu, 16 Apr 2020 00:49:13: 47000000 INFO @ Thu, 16 Apr 2020 00:49:13: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:49:13: 47000000 INFO @ Thu, 16 Apr 2020 00:49:23: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:49:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:49:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.05_summits.bed INFO @ Thu, 16 Apr 2020 00:49:23: Done! INFO @ Thu, 16 Apr 2020 00:49:23: 48000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Thu, 16 Apr 2020 00:49:24: 48000000 pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (556 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:49:34: 49000000 INFO @ Thu, 16 Apr 2020 00:49:35: 49000000 INFO @ Thu, 16 Apr 2020 00:49:45: 50000000 INFO @ Thu, 16 Apr 2020 00:49:45: 50000000 INFO @ Thu, 16 Apr 2020 00:49:55: 51000000 INFO @ Thu, 16 Apr 2020 00:49:56: 51000000 INFO @ Thu, 16 Apr 2020 00:50:06: 52000000 INFO @ Thu, 16 Apr 2020 00:50:07: 52000000 INFO @ Thu, 16 Apr 2020 00:50:17: 53000000 INFO @ Thu, 16 Apr 2020 00:50:17: 53000000 INFO @ Thu, 16 Apr 2020 00:50:23: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:50:23: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:50:23: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:50:23: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:50:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:50:23: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:50:23: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:50:23: #1 finished! INFO @ Thu, 16 Apr 2020 00:50:23: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:50:23: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:50:23: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:50:23: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:50:23: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:50:23: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:50:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:50:24: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:50:24: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:50:24: #1 finished! INFO @ Thu, 16 Apr 2020 00:50:24: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:50:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:50:24: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:50:24: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:50:24: start model_add_line... INFO @ Thu, 16 Apr 2020 00:50:24: start X-correlation... INFO @ Thu, 16 Apr 2020 00:50:24: end of X-cor INFO @ Thu, 16 Apr 2020 00:50:24: #2 finished! INFO @ Thu, 16 Apr 2020 00:50:24: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:50:24: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:50:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10_model.r WARNING @ Thu, 16 Apr 2020 00:50:24: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:50:24: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:50:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:50:24: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:50:24: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:50:24: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:50:24: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:50:24: start model_add_line... INFO @ Thu, 16 Apr 2020 00:50:24: start X-correlation... INFO @ Thu, 16 Apr 2020 00:50:24: end of X-cor INFO @ Thu, 16 Apr 2020 00:50:24: #2 finished! INFO @ Thu, 16 Apr 2020 00:50:24: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:50:24: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:50:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20_model.r WARNING @ Thu, 16 Apr 2020 00:50:24: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:50:24: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:50:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:50:24: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:50:24: #3 Pre-compute pvalue-qvalue table... BigWig に変換しました。 INFO @ Thu, 16 Apr 2020 00:50:46: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:50:46: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:50:56: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:50:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:50:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.10_summits.bed INFO @ Thu, 16 Apr 2020 00:50:56: Done! INFO @ Thu, 16 Apr 2020 00:50:57: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:50:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:50:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619583/SRX6619583.20_summits.bed INFO @ Thu, 16 Apr 2020 00:50:57: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (367 records, 4 fields): 2 millis CompletedMACS2peakCalling pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (228 records, 4 fields): 2 millis CompletedMACS2peakCalling