Job ID = 5720209 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:58:46 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:08:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:28:59 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:33:04 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:36:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:39:44 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:42:59 fasterq-dump.2.9.6 sys: error unknown while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 38,061,850 reads read : 76,123,700 reads written : 76,123,700 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:08:09 38061850 reads; of these: 38061850 (100.00%) were paired; of these: 19380337 (50.92%) aligned concordantly 0 times 15384243 (40.42%) aligned concordantly exactly 1 time 3297270 (8.66%) aligned concordantly >1 times ---- 19380337 pairs aligned concordantly 0 times; of these: 9023135 (46.56%) aligned discordantly 1 time ---- 10357202 pairs aligned 0 times concordantly or discordantly; of these: 20714404 mates make up the pairs; of these: 17433285 (84.16%) aligned 0 times 1327117 (6.41%) aligned exactly 1 time 1954002 (9.43%) aligned >1 times 77.10% overall alignment rate Time searching: 01:08:09 Overall time: 01:08:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3400562 / 27223872 = 0.1249 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:20:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:20:00: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:20:00: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:20:07: 1000000 INFO @ Thu, 16 Apr 2020 00:20:14: 2000000 INFO @ Thu, 16 Apr 2020 00:20:21: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:20:28: 4000000 INFO @ Thu, 16 Apr 2020 00:20:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:20:30: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:20:30: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:20:36: 5000000 INFO @ Thu, 16 Apr 2020 00:20:38: 1000000 INFO @ Thu, 16 Apr 2020 00:20:44: 6000000 INFO @ Thu, 16 Apr 2020 00:20:46: 2000000 INFO @ Thu, 16 Apr 2020 00:20:52: 7000000 INFO @ Thu, 16 Apr 2020 00:20:54: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:20:59: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:20:59: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:20:59: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:21:00: 8000000 INFO @ Thu, 16 Apr 2020 00:21:02: 4000000 INFO @ Thu, 16 Apr 2020 00:21:08: 1000000 INFO @ Thu, 16 Apr 2020 00:21:08: 9000000 INFO @ Thu, 16 Apr 2020 00:21:11: 5000000 INFO @ Thu, 16 Apr 2020 00:21:16: 2000000 INFO @ Thu, 16 Apr 2020 00:21:16: 10000000 INFO @ Thu, 16 Apr 2020 00:21:19: 6000000 INFO @ Thu, 16 Apr 2020 00:21:24: 3000000 INFO @ Thu, 16 Apr 2020 00:21:24: 11000000 INFO @ Thu, 16 Apr 2020 00:21:27: 7000000 INFO @ Thu, 16 Apr 2020 00:21:32: 4000000 INFO @ Thu, 16 Apr 2020 00:21:32: 12000000 INFO @ Thu, 16 Apr 2020 00:21:35: 8000000 INFO @ Thu, 16 Apr 2020 00:21:40: 5000000 INFO @ Thu, 16 Apr 2020 00:21:40: 13000000 INFO @ Thu, 16 Apr 2020 00:21:43: 9000000 INFO @ Thu, 16 Apr 2020 00:21:48: 6000000 INFO @ Thu, 16 Apr 2020 00:21:49: 14000000 INFO @ Thu, 16 Apr 2020 00:21:51: 10000000 INFO @ Thu, 16 Apr 2020 00:21:56: 7000000 INFO @ Thu, 16 Apr 2020 00:21:57: 15000000 INFO @ Thu, 16 Apr 2020 00:21:59: 11000000 INFO @ Thu, 16 Apr 2020 00:22:04: 8000000 INFO @ Thu, 16 Apr 2020 00:22:05: 16000000 INFO @ Thu, 16 Apr 2020 00:22:06: 12000000 INFO @ Thu, 16 Apr 2020 00:22:12: 9000000 INFO @ Thu, 16 Apr 2020 00:22:13: 17000000 INFO @ Thu, 16 Apr 2020 00:22:14: 13000000 INFO @ Thu, 16 Apr 2020 00:22:20: 10000000 INFO @ Thu, 16 Apr 2020 00:22:21: 18000000 INFO @ Thu, 16 Apr 2020 00:22:22: 14000000 INFO @ Thu, 16 Apr 2020 00:22:28: 11000000 INFO @ Thu, 16 Apr 2020 00:22:29: 19000000 INFO @ Thu, 16 Apr 2020 00:22:30: 15000000 INFO @ Thu, 16 Apr 2020 00:22:36: 12000000 INFO @ Thu, 16 Apr 2020 00:22:36: 20000000 INFO @ Thu, 16 Apr 2020 00:22:38: 16000000 INFO @ Thu, 16 Apr 2020 00:22:44: 13000000 INFO @ Thu, 16 Apr 2020 00:22:44: 21000000 INFO @ Thu, 16 Apr 2020 00:22:46: 17000000 INFO @ Thu, 16 Apr 2020 00:22:52: 14000000 INFO @ Thu, 16 Apr 2020 00:22:52: 22000000 INFO @ Thu, 16 Apr 2020 00:22:54: 18000000 INFO @ Thu, 16 Apr 2020 00:23:00: 15000000 INFO @ Thu, 16 Apr 2020 00:23:00: 23000000 INFO @ Thu, 16 Apr 2020 00:23:02: 19000000 INFO @ Thu, 16 Apr 2020 00:23:08: 16000000 INFO @ Thu, 16 Apr 2020 00:23:08: 24000000 INFO @ Thu, 16 Apr 2020 00:23:10: 20000000 INFO @ Thu, 16 Apr 2020 00:23:16: 17000000 INFO @ Thu, 16 Apr 2020 00:23:16: 25000000 INFO @ Thu, 16 Apr 2020 00:23:17: 21000000 INFO @ Thu, 16 Apr 2020 00:23:24: 18000000 INFO @ Thu, 16 Apr 2020 00:23:24: 26000000 INFO @ Thu, 16 Apr 2020 00:23:25: 22000000 INFO @ Thu, 16 Apr 2020 00:23:32: 19000000 INFO @ Thu, 16 Apr 2020 00:23:32: 27000000 INFO @ Thu, 16 Apr 2020 00:23:33: 23000000 INFO @ Thu, 16 Apr 2020 00:23:40: 20000000 INFO @ Thu, 16 Apr 2020 00:23:40: 28000000 INFO @ Thu, 16 Apr 2020 00:23:41: 24000000 INFO @ Thu, 16 Apr 2020 00:23:48: 21000000 INFO @ Thu, 16 Apr 2020 00:23:48: 29000000 INFO @ Thu, 16 Apr 2020 00:23:49: 25000000 INFO @ Thu, 16 Apr 2020 00:23:56: 22000000 INFO @ Thu, 16 Apr 2020 00:23:56: 30000000 INFO @ Thu, 16 Apr 2020 00:23:57: 26000000 INFO @ Thu, 16 Apr 2020 00:24:04: 23000000 INFO @ Thu, 16 Apr 2020 00:24:04: 31000000 INFO @ Thu, 16 Apr 2020 00:24:05: 27000000 INFO @ Thu, 16 Apr 2020 00:24:12: 24000000 INFO @ Thu, 16 Apr 2020 00:24:12: 32000000 INFO @ Thu, 16 Apr 2020 00:24:13: 28000000 INFO @ Thu, 16 Apr 2020 00:24:20: 25000000 INFO @ Thu, 16 Apr 2020 00:24:20: 33000000 INFO @ Thu, 16 Apr 2020 00:24:21: 29000000 INFO @ Thu, 16 Apr 2020 00:24:28: 26000000 INFO @ Thu, 16 Apr 2020 00:24:28: 34000000 INFO @ Thu, 16 Apr 2020 00:24:29: 30000000 INFO @ Thu, 16 Apr 2020 00:24:36: 27000000 INFO @ Thu, 16 Apr 2020 00:24:36: 35000000 INFO @ Thu, 16 Apr 2020 00:24:37: 31000000 INFO @ Thu, 16 Apr 2020 00:24:43: 28000000 INFO @ Thu, 16 Apr 2020 00:24:44: 36000000 INFO @ Thu, 16 Apr 2020 00:24:45: 32000000 INFO @ Thu, 16 Apr 2020 00:24:51: 29000000 INFO @ Thu, 16 Apr 2020 00:24:52: 37000000 INFO @ Thu, 16 Apr 2020 00:24:53: 33000000 INFO @ Thu, 16 Apr 2020 00:24:59: 30000000 INFO @ Thu, 16 Apr 2020 00:25:00: 38000000 INFO @ Thu, 16 Apr 2020 00:25:01: 34000000 INFO @ Thu, 16 Apr 2020 00:25:07: 31000000 INFO @ Thu, 16 Apr 2020 00:25:08: 39000000 INFO @ Thu, 16 Apr 2020 00:25:09: 35000000 INFO @ Thu, 16 Apr 2020 00:25:15: 40000000 INFO @ Thu, 16 Apr 2020 00:25:16: 32000000 INFO @ Thu, 16 Apr 2020 00:25:17: 36000000 INFO @ Thu, 16 Apr 2020 00:25:23: 41000000 INFO @ Thu, 16 Apr 2020 00:25:24: 33000000 INFO @ Thu, 16 Apr 2020 00:25:25: 37000000 INFO @ Thu, 16 Apr 2020 00:25:31: 42000000 INFO @ Thu, 16 Apr 2020 00:25:32: 34000000 INFO @ Thu, 16 Apr 2020 00:25:33: 38000000 INFO @ Thu, 16 Apr 2020 00:25:39: 43000000 INFO @ Thu, 16 Apr 2020 00:25:40: 35000000 INFO @ Thu, 16 Apr 2020 00:25:40: 39000000 INFO @ Thu, 16 Apr 2020 00:25:47: 44000000 INFO @ Thu, 16 Apr 2020 00:25:48: 36000000 INFO @ Thu, 16 Apr 2020 00:25:48: 40000000 INFO @ Thu, 16 Apr 2020 00:25:55: 45000000 INFO @ Thu, 16 Apr 2020 00:25:56: 37000000 INFO @ Thu, 16 Apr 2020 00:25:56: 41000000 INFO @ Thu, 16 Apr 2020 00:26:03: 46000000 INFO @ Thu, 16 Apr 2020 00:26:03: 38000000 INFO @ Thu, 16 Apr 2020 00:26:04: 42000000 INFO @ Thu, 16 Apr 2020 00:26:10: 47000000 INFO @ Thu, 16 Apr 2020 00:26:11: 39000000 INFO @ Thu, 16 Apr 2020 00:26:12: 43000000 INFO @ Thu, 16 Apr 2020 00:26:18: 48000000 INFO @ Thu, 16 Apr 2020 00:26:19: 40000000 INFO @ Thu, 16 Apr 2020 00:26:20: 44000000 INFO @ Thu, 16 Apr 2020 00:26:26: 49000000 INFO @ Thu, 16 Apr 2020 00:26:27: 41000000 INFO @ Thu, 16 Apr 2020 00:26:28: 45000000 INFO @ Thu, 16 Apr 2020 00:26:34: 50000000 INFO @ Thu, 16 Apr 2020 00:26:35: 42000000 INFO @ Thu, 16 Apr 2020 00:26:36: 46000000 INFO @ Thu, 16 Apr 2020 00:26:42: 51000000 INFO @ Thu, 16 Apr 2020 00:26:43: 43000000 INFO @ Thu, 16 Apr 2020 00:26:44: 47000000 INFO @ Thu, 16 Apr 2020 00:26:49: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:26:49: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:26:49: #1 total tags in treatment: 16178552 INFO @ Thu, 16 Apr 2020 00:26:49: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:26:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:26:50: #1 tags after filtering in treatment: 13580597 INFO @ Thu, 16 Apr 2020 00:26:50: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:26:50: #1 finished! INFO @ Thu, 16 Apr 2020 00:26:50: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:26:50: #2 number of paired peaks: 264 WARNING @ Thu, 16 Apr 2020 00:26:50: Fewer paired peaks (264) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 264 pairs to build model! INFO @ Thu, 16 Apr 2020 00:26:50: start model_add_line... INFO @ Thu, 16 Apr 2020 00:26:51: start X-correlation... INFO @ Thu, 16 Apr 2020 00:26:51: end of X-cor INFO @ Thu, 16 Apr 2020 00:26:51: #2 finished! INFO @ Thu, 16 Apr 2020 00:26:51: #2 predicted fragment length is 198 bps INFO @ Thu, 16 Apr 2020 00:26:51: #2 alternative fragment length(s) may be 198 bps INFO @ Thu, 16 Apr 2020 00:26:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05_model.r WARNING @ Thu, 16 Apr 2020 00:26:51: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:26:51: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Thu, 16 Apr 2020 00:26:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:26:51: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:26:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:26:51: 44000000 INFO @ Thu, 16 Apr 2020 00:26:52: 48000000 INFO @ Thu, 16 Apr 2020 00:26:59: 45000000 INFO @ Thu, 16 Apr 2020 00:27:00: 49000000 INFO @ Thu, 16 Apr 2020 00:27:07: 46000000 INFO @ Thu, 16 Apr 2020 00:27:07: 50000000 INFO @ Thu, 16 Apr 2020 00:27:14: 47000000 INFO @ Thu, 16 Apr 2020 00:27:15: 51000000 INFO @ Thu, 16 Apr 2020 00:27:17: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:27:22: 48000000 INFO @ Thu, 16 Apr 2020 00:27:22: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:27:22: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:27:22: #1 total tags in treatment: 16178552 INFO @ Thu, 16 Apr 2020 00:27:22: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:27:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:27:23: #1 tags after filtering in treatment: 13580597 INFO @ Thu, 16 Apr 2020 00:27:23: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:27:23: #1 finished! INFO @ Thu, 16 Apr 2020 00:27:23: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:27:23: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:27:24: #2 number of paired peaks: 264 WARNING @ Thu, 16 Apr 2020 00:27:24: Fewer paired peaks (264) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 264 pairs to build model! INFO @ Thu, 16 Apr 2020 00:27:24: start model_add_line... INFO @ Thu, 16 Apr 2020 00:27:24: start X-correlation... INFO @ Thu, 16 Apr 2020 00:27:24: end of X-cor INFO @ Thu, 16 Apr 2020 00:27:24: #2 finished! INFO @ Thu, 16 Apr 2020 00:27:24: #2 predicted fragment length is 198 bps INFO @ Thu, 16 Apr 2020 00:27:24: #2 alternative fragment length(s) may be 198 bps INFO @ Thu, 16 Apr 2020 00:27:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10_model.r WARNING @ Thu, 16 Apr 2020 00:27:24: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:27:24: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Thu, 16 Apr 2020 00:27:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:27:24: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:27:24: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:27:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:27:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:27:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.05_summits.bed INFO @ Thu, 16 Apr 2020 00:27:29: Done! INFO @ Thu, 16 Apr 2020 00:27:30: 49000000 pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (577 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:27:37: 50000000 INFO @ Thu, 16 Apr 2020 00:27:45: 51000000 INFO @ Thu, 16 Apr 2020 00:27:50: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:27:51: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:27:51: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:27:51: #1 total tags in treatment: 16178552 INFO @ Thu, 16 Apr 2020 00:27:51: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:27:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:27:51: #1 tags after filtering in treatment: 13580597 INFO @ Thu, 16 Apr 2020 00:27:51: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:27:51: #1 finished! INFO @ Thu, 16 Apr 2020 00:27:51: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:27:51: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:27:52: #2 number of paired peaks: 264 WARNING @ Thu, 16 Apr 2020 00:27:52: Fewer paired peaks (264) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 264 pairs to build model! INFO @ Thu, 16 Apr 2020 00:27:52: start model_add_line... INFO @ Thu, 16 Apr 2020 00:27:52: start X-correlation... INFO @ Thu, 16 Apr 2020 00:27:52: end of X-cor INFO @ Thu, 16 Apr 2020 00:27:52: #2 finished! INFO @ Thu, 16 Apr 2020 00:27:52: #2 predicted fragment length is 198 bps INFO @ Thu, 16 Apr 2020 00:27:52: #2 alternative fragment length(s) may be 198 bps INFO @ Thu, 16 Apr 2020 00:27:52: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20_model.r WARNING @ Thu, 16 Apr 2020 00:27:52: #2 Since the d (198) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:27:52: #2 You may need to consider one of the other alternative d(s): 198 WARNING @ Thu, 16 Apr 2020 00:27:52: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:27:52: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:27:52: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:28:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:28:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:28:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.10_summits.bed INFO @ Thu, 16 Apr 2020 00:28:02: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (377 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:28:19: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:28:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:28:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:28:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619577/SRX6619577.20_summits.bed INFO @ Thu, 16 Apr 2020 00:28:31: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (239 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。