Job ID = 5720185 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T13:16:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:29:22 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 43,555,027 reads read : 87,110,054 reads written : 87,110,054 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:37:29 43555027 reads; of these: 43555027 (100.00%) were paired; of these: 27937589 (64.14%) aligned concordantly 0 times 12830737 (29.46%) aligned concordantly exactly 1 time 2786701 (6.40%) aligned concordantly >1 times ---- 27937589 pairs aligned concordantly 0 times; of these: 14023312 (50.20%) aligned discordantly 1 time ---- 13914277 pairs aligned 0 times concordantly or discordantly; of these: 27828554 mates make up the pairs; of these: 24164934 (86.84%) aligned 0 times 1279023 (4.60%) aligned exactly 1 time 2384597 (8.57%) aligned >1 times 72.26% overall alignment rate Time searching: 01:37:30 Overall time: 01:37:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 48 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4682143 / 29063535 = 0.1611 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:48:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:48:12: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:48:12: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:48:21: 1000000 INFO @ Thu, 16 Apr 2020 00:48:30: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:48:39: 3000000 INFO @ Thu, 16 Apr 2020 00:48:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:48:40: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:48:40: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:48:49: 4000000 INFO @ Thu, 16 Apr 2020 00:48:51: 1000000 INFO @ Thu, 16 Apr 2020 00:49:00: 5000000 INFO @ Thu, 16 Apr 2020 00:49:02: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:49:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:49:10: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:49:10: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:49:10: 6000000 INFO @ Thu, 16 Apr 2020 00:49:11: 3000000 INFO @ Thu, 16 Apr 2020 00:49:18: 1000000 INFO @ Thu, 16 Apr 2020 00:49:20: 7000000 INFO @ Thu, 16 Apr 2020 00:49:21: 4000000 INFO @ Thu, 16 Apr 2020 00:49:27: 2000000 INFO @ Thu, 16 Apr 2020 00:49:30: 8000000 INFO @ Thu, 16 Apr 2020 00:49:30: 5000000 INFO @ Thu, 16 Apr 2020 00:49:35: 3000000 INFO @ Thu, 16 Apr 2020 00:49:40: 9000000 INFO @ Thu, 16 Apr 2020 00:49:41: 6000000 INFO @ Thu, 16 Apr 2020 00:49:43: 4000000 INFO @ Thu, 16 Apr 2020 00:49:51: 10000000 INFO @ Thu, 16 Apr 2020 00:49:51: 7000000 INFO @ Thu, 16 Apr 2020 00:49:52: 5000000 INFO @ Thu, 16 Apr 2020 00:50:01: 8000000 INFO @ Thu, 16 Apr 2020 00:50:01: 6000000 INFO @ Thu, 16 Apr 2020 00:50:01: 11000000 INFO @ Thu, 16 Apr 2020 00:50:11: 7000000 INFO @ Thu, 16 Apr 2020 00:50:11: 9000000 INFO @ Thu, 16 Apr 2020 00:50:11: 12000000 INFO @ Thu, 16 Apr 2020 00:50:19: 8000000 INFO @ Thu, 16 Apr 2020 00:50:21: 10000000 INFO @ Thu, 16 Apr 2020 00:50:21: 13000000 INFO @ Thu, 16 Apr 2020 00:50:28: 9000000 INFO @ Thu, 16 Apr 2020 00:50:31: 14000000 INFO @ Thu, 16 Apr 2020 00:50:31: 11000000 INFO @ Thu, 16 Apr 2020 00:50:36: 10000000 INFO @ Thu, 16 Apr 2020 00:50:41: 15000000 INFO @ Thu, 16 Apr 2020 00:50:42: 12000000 INFO @ Thu, 16 Apr 2020 00:50:44: 11000000 INFO @ Thu, 16 Apr 2020 00:50:52: 16000000 INFO @ Thu, 16 Apr 2020 00:50:53: 12000000 INFO @ Thu, 16 Apr 2020 00:50:53: 13000000 INFO @ Thu, 16 Apr 2020 00:51:01: 13000000 INFO @ Thu, 16 Apr 2020 00:51:02: 17000000 INFO @ Thu, 16 Apr 2020 00:51:03: 14000000 INFO @ Thu, 16 Apr 2020 00:51:09: 14000000 INFO @ Thu, 16 Apr 2020 00:51:12: 18000000 INFO @ Thu, 16 Apr 2020 00:51:13: 15000000 INFO @ Thu, 16 Apr 2020 00:51:18: 15000000 INFO @ Thu, 16 Apr 2020 00:51:22: 19000000 INFO @ Thu, 16 Apr 2020 00:51:23: 16000000 INFO @ Thu, 16 Apr 2020 00:51:26: 16000000 INFO @ Thu, 16 Apr 2020 00:51:32: 20000000 INFO @ Thu, 16 Apr 2020 00:51:33: 17000000 INFO @ Thu, 16 Apr 2020 00:51:34: 17000000 INFO @ Thu, 16 Apr 2020 00:51:41: 21000000 INFO @ Thu, 16 Apr 2020 00:51:43: 18000000 INFO @ Thu, 16 Apr 2020 00:51:43: 18000000 INFO @ Thu, 16 Apr 2020 00:51:51: 19000000 INFO @ Thu, 16 Apr 2020 00:51:51: 22000000 INFO @ Thu, 16 Apr 2020 00:51:53: 19000000 INFO @ Thu, 16 Apr 2020 00:51:59: 20000000 INFO @ Thu, 16 Apr 2020 00:52:01: 23000000 INFO @ Thu, 16 Apr 2020 00:52:03: 20000000 INFO @ Thu, 16 Apr 2020 00:52:08: 21000000 INFO @ Thu, 16 Apr 2020 00:52:10: 24000000 INFO @ Thu, 16 Apr 2020 00:52:13: 21000000 INFO @ Thu, 16 Apr 2020 00:52:16: 22000000 INFO @ Thu, 16 Apr 2020 00:52:20: 25000000 INFO @ Thu, 16 Apr 2020 00:52:23: 22000000 INFO @ Thu, 16 Apr 2020 00:52:24: 23000000 INFO @ Thu, 16 Apr 2020 00:52:30: 26000000 INFO @ Thu, 16 Apr 2020 00:52:32: 23000000 INFO @ Thu, 16 Apr 2020 00:52:33: 24000000 INFO @ Thu, 16 Apr 2020 00:52:39: 27000000 INFO @ Thu, 16 Apr 2020 00:52:41: 25000000 INFO @ Thu, 16 Apr 2020 00:52:42: 24000000 INFO @ Thu, 16 Apr 2020 00:52:49: 28000000 INFO @ Thu, 16 Apr 2020 00:52:49: 26000000 INFO @ Thu, 16 Apr 2020 00:52:52: 25000000 INFO @ Thu, 16 Apr 2020 00:52:58: 27000000 INFO @ Thu, 16 Apr 2020 00:52:58: 29000000 INFO @ Thu, 16 Apr 2020 00:53:02: 26000000 INFO @ Thu, 16 Apr 2020 00:53:06: 28000000 INFO @ Thu, 16 Apr 2020 00:53:08: 30000000 INFO @ Thu, 16 Apr 2020 00:53:12: 27000000 INFO @ Thu, 16 Apr 2020 00:53:14: 29000000 INFO @ Thu, 16 Apr 2020 00:53:18: 31000000 INFO @ Thu, 16 Apr 2020 00:53:22: 28000000 INFO @ Thu, 16 Apr 2020 00:53:23: 30000000 INFO @ Thu, 16 Apr 2020 00:53:28: 32000000 INFO @ Thu, 16 Apr 2020 00:53:32: 31000000 INFO @ Thu, 16 Apr 2020 00:53:32: 29000000 INFO @ Thu, 16 Apr 2020 00:53:37: 33000000 INFO @ Thu, 16 Apr 2020 00:53:41: 32000000 INFO @ Thu, 16 Apr 2020 00:53:42: 30000000 INFO @ Thu, 16 Apr 2020 00:53:47: 34000000 INFO @ Thu, 16 Apr 2020 00:53:50: 33000000 INFO @ Thu, 16 Apr 2020 00:53:52: 31000000 INFO @ Thu, 16 Apr 2020 00:53:56: 35000000 INFO @ Thu, 16 Apr 2020 00:54:00: 34000000 INFO @ Thu, 16 Apr 2020 00:54:02: 32000000 INFO @ Thu, 16 Apr 2020 00:54:06: 36000000 INFO @ Thu, 16 Apr 2020 00:54:08: 35000000 INFO @ Thu, 16 Apr 2020 00:54:12: 33000000 INFO @ Thu, 16 Apr 2020 00:54:15: 37000000 INFO @ Thu, 16 Apr 2020 00:54:17: 36000000 INFO @ Thu, 16 Apr 2020 00:54:21: 34000000 INFO @ Thu, 16 Apr 2020 00:54:25: 38000000 INFO @ Thu, 16 Apr 2020 00:54:25: 37000000 INFO @ Thu, 16 Apr 2020 00:54:31: 35000000 INFO @ Thu, 16 Apr 2020 00:54:33: 38000000 INFO @ Thu, 16 Apr 2020 00:54:34: 39000000 INFO @ Thu, 16 Apr 2020 00:54:41: 36000000 INFO @ Thu, 16 Apr 2020 00:54:41: 39000000 INFO @ Thu, 16 Apr 2020 00:54:44: 40000000 INFO @ Thu, 16 Apr 2020 00:54:49: 40000000 INFO @ Thu, 16 Apr 2020 00:54:51: 37000000 INFO @ Thu, 16 Apr 2020 00:54:53: 41000000 INFO @ Thu, 16 Apr 2020 00:54:57: 41000000 INFO @ Thu, 16 Apr 2020 00:55:00: 38000000 INFO @ Thu, 16 Apr 2020 00:55:02: 42000000 INFO @ Thu, 16 Apr 2020 00:55:06: 42000000 INFO @ Thu, 16 Apr 2020 00:55:10: 39000000 INFO @ Thu, 16 Apr 2020 00:55:12: 43000000 INFO @ Thu, 16 Apr 2020 00:55:14: 43000000 INFO @ Thu, 16 Apr 2020 00:55:19: 40000000 INFO @ Thu, 16 Apr 2020 00:55:21: 44000000 INFO @ Thu, 16 Apr 2020 00:55:22: 44000000 INFO @ Thu, 16 Apr 2020 00:55:29: 41000000 INFO @ Thu, 16 Apr 2020 00:55:31: 45000000 INFO @ Thu, 16 Apr 2020 00:55:31: 45000000 INFO @ Thu, 16 Apr 2020 00:55:39: 42000000 INFO @ Thu, 16 Apr 2020 00:55:39: 46000000 INFO @ Thu, 16 Apr 2020 00:55:40: 46000000 INFO @ Thu, 16 Apr 2020 00:55:47: 47000000 INFO @ Thu, 16 Apr 2020 00:55:49: 43000000 INFO @ Thu, 16 Apr 2020 00:55:51: 47000000 INFO @ Thu, 16 Apr 2020 00:55:55: 48000000 INFO @ Thu, 16 Apr 2020 00:56:00: 44000000 INFO @ Thu, 16 Apr 2020 00:56:01: 48000000 INFO @ Thu, 16 Apr 2020 00:56:04: 49000000 INFO @ Thu, 16 Apr 2020 00:56:10: 45000000 INFO @ Thu, 16 Apr 2020 00:56:11: 49000000 INFO @ Thu, 16 Apr 2020 00:56:12: 50000000 INFO @ Thu, 16 Apr 2020 00:56:20: 46000000 INFO @ Thu, 16 Apr 2020 00:56:20: 51000000 INFO @ Thu, 16 Apr 2020 00:56:21: 50000000 INFO @ Thu, 16 Apr 2020 00:56:28: 52000000 INFO @ Thu, 16 Apr 2020 00:56:30: 47000000 INFO @ Thu, 16 Apr 2020 00:56:30: 51000000 INFO @ Thu, 16 Apr 2020 00:56:37: 53000000 INFO @ Thu, 16 Apr 2020 00:56:39: 48000000 INFO @ Thu, 16 Apr 2020 00:56:39: 52000000 INFO @ Thu, 16 Apr 2020 00:56:41: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:56:41: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:56:41: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:56:41: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:56:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:56:42: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:56:42: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:56:42: #1 finished! INFO @ Thu, 16 Apr 2020 00:56:42: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:56:42: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:56:42: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:56:42: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:56:42: start model_add_line... INFO @ Thu, 16 Apr 2020 00:56:43: start X-correlation... INFO @ Thu, 16 Apr 2020 00:56:43: end of X-cor INFO @ Thu, 16 Apr 2020 00:56:43: #2 finished! INFO @ Thu, 16 Apr 2020 00:56:43: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:56:43: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:56:43: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20_model.r WARNING @ Thu, 16 Apr 2020 00:56:43: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:56:43: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:56:43: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:56:43: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:56:43: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:56:49: 53000000 INFO @ Thu, 16 Apr 2020 00:56:49: 49000000 INFO @ Thu, 16 Apr 2020 00:56:54: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:56:54: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:56:54: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:56:54: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:56:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:56:55: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:56:55: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:56:55: #1 finished! INFO @ Thu, 16 Apr 2020 00:56:55: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:56:55: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:56:55: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:56:55: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:56:55: start model_add_line... INFO @ Thu, 16 Apr 2020 00:56:55: start X-correlation... INFO @ Thu, 16 Apr 2020 00:56:55: end of X-cor INFO @ Thu, 16 Apr 2020 00:56:55: #2 finished! INFO @ Thu, 16 Apr 2020 00:56:55: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:56:55: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:56:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05_model.r WARNING @ Thu, 16 Apr 2020 00:56:55: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:56:55: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:56:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:56:55: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:56:55: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:56:58: 50000000 INFO @ Thu, 16 Apr 2020 00:57:04: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:57:07: 51000000 INFO @ Thu, 16 Apr 2020 00:57:15: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:57:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:57:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.20_summits.bed INFO @ Thu, 16 Apr 2020 00:57:15: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (228 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:57:15: 52000000 INFO @ Thu, 16 Apr 2020 00:57:17: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:57:24: 53000000 INFO @ Thu, 16 Apr 2020 00:57:28: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:57:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:57:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.05_summits.bed INFO @ Thu, 16 Apr 2020 00:57:28: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (556 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:57:28: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:57:28: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:57:28: #1 total tags in treatment: 12888137 INFO @ Thu, 16 Apr 2020 00:57:28: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:57:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:57:29: #1 tags after filtering in treatment: 10822475 INFO @ Thu, 16 Apr 2020 00:57:29: #1 Redundant rate of treatment: 0.16 INFO @ Thu, 16 Apr 2020 00:57:29: #1 finished! INFO @ Thu, 16 Apr 2020 00:57:29: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:57:29: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:57:29: #2 number of paired peaks: 297 WARNING @ Thu, 16 Apr 2020 00:57:29: Fewer paired peaks (297) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 297 pairs to build model! INFO @ Thu, 16 Apr 2020 00:57:29: start model_add_line... INFO @ Thu, 16 Apr 2020 00:57:30: start X-correlation... INFO @ Thu, 16 Apr 2020 00:57:30: end of X-cor INFO @ Thu, 16 Apr 2020 00:57:30: #2 finished! INFO @ Thu, 16 Apr 2020 00:57:30: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:57:30: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:57:30: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10_model.r WARNING @ Thu, 16 Apr 2020 00:57:30: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:57:30: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:57:30: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:57:30: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:57:30: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:57:51: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:58:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:58:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:58:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619555/SRX6619555.10_summits.bed INFO @ Thu, 16 Apr 2020 00:58:03: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (367 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。