Job ID = 5720176 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:30:00 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:48 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 37,857,932 reads read : 75,715,864 reads written : 75,715,864 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:26:37 37857932 reads; of these: 37857932 (100.00%) were paired; of these: 21367859 (56.44%) aligned concordantly 0 times 13785348 (36.41%) aligned concordantly exactly 1 time 2704725 (7.14%) aligned concordantly >1 times ---- 21367859 pairs aligned concordantly 0 times; of these: 10641146 (49.80%) aligned discordantly 1 time ---- 10726713 pairs aligned 0 times concordantly or discordantly; of these: 21453426 mates make up the pairs; of these: 17555828 (81.83%) aligned 0 times 1868382 (8.71%) aligned exactly 1 time 2029216 (9.46%) aligned >1 times 76.81% overall alignment rate Time searching: 01:26:37 Overall time: 01:26:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 4245014 / 26460353 = 0.1604 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:33:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:33:50: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:33:50: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:34:00: 1000000 INFO @ Thu, 16 Apr 2020 00:34:12: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:34:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:34:20: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:34:20: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:34:24: 3000000 INFO @ Thu, 16 Apr 2020 00:34:28: 1000000 INFO @ Thu, 16 Apr 2020 00:34:34: 4000000 INFO @ Thu, 16 Apr 2020 00:34:37: 2000000 INFO @ Thu, 16 Apr 2020 00:34:43: 5000000 INFO @ Thu, 16 Apr 2020 00:34:46: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:34:50: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:34:50: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:34:50: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:34:52: 6000000 INFO @ Thu, 16 Apr 2020 00:34:54: 4000000 INFO @ Thu, 16 Apr 2020 00:35:00: 1000000 INFO @ Thu, 16 Apr 2020 00:35:01: 7000000 INFO @ Thu, 16 Apr 2020 00:35:03: 5000000 INFO @ Thu, 16 Apr 2020 00:35:10: 8000000 INFO @ Thu, 16 Apr 2020 00:35:11: 2000000 INFO @ Thu, 16 Apr 2020 00:35:12: 6000000 INFO @ Thu, 16 Apr 2020 00:35:18: 9000000 INFO @ Thu, 16 Apr 2020 00:35:20: 7000000 INFO @ Thu, 16 Apr 2020 00:35:22: 3000000 INFO @ Thu, 16 Apr 2020 00:35:28: 10000000 INFO @ Thu, 16 Apr 2020 00:35:29: 8000000 INFO @ Thu, 16 Apr 2020 00:35:33: 4000000 INFO @ Thu, 16 Apr 2020 00:35:36: 11000000 INFO @ Thu, 16 Apr 2020 00:35:38: 9000000 INFO @ Thu, 16 Apr 2020 00:35:43: 5000000 INFO @ Thu, 16 Apr 2020 00:35:45: 12000000 INFO @ Thu, 16 Apr 2020 00:35:46: 10000000 INFO @ Thu, 16 Apr 2020 00:35:52: 6000000 INFO @ Thu, 16 Apr 2020 00:35:54: 13000000 INFO @ Thu, 16 Apr 2020 00:35:55: 11000000 INFO @ Thu, 16 Apr 2020 00:36:00: 7000000 INFO @ Thu, 16 Apr 2020 00:36:03: 14000000 INFO @ Thu, 16 Apr 2020 00:36:03: 12000000 INFO @ Thu, 16 Apr 2020 00:36:10: 8000000 INFO @ Thu, 16 Apr 2020 00:36:12: 15000000 INFO @ Thu, 16 Apr 2020 00:36:12: 13000000 INFO @ Thu, 16 Apr 2020 00:36:19: 9000000 INFO @ Thu, 16 Apr 2020 00:36:20: 14000000 INFO @ Thu, 16 Apr 2020 00:36:20: 16000000 INFO @ Thu, 16 Apr 2020 00:36:28: 10000000 INFO @ Thu, 16 Apr 2020 00:36:29: 15000000 INFO @ Thu, 16 Apr 2020 00:36:30: 17000000 INFO @ Thu, 16 Apr 2020 00:36:37: 11000000 INFO @ Thu, 16 Apr 2020 00:36:39: 16000000 INFO @ Thu, 16 Apr 2020 00:36:39: 18000000 INFO @ Thu, 16 Apr 2020 00:36:47: 12000000 INFO @ Thu, 16 Apr 2020 00:36:47: 17000000 INFO @ Thu, 16 Apr 2020 00:36:48: 19000000 INFO @ Thu, 16 Apr 2020 00:36:56: 18000000 INFO @ Thu, 16 Apr 2020 00:36:56: 13000000 INFO @ Thu, 16 Apr 2020 00:36:57: 20000000 INFO @ Thu, 16 Apr 2020 00:37:04: 19000000 INFO @ Thu, 16 Apr 2020 00:37:05: 14000000 INFO @ Thu, 16 Apr 2020 00:37:05: 21000000 INFO @ Thu, 16 Apr 2020 00:37:12: 20000000 INFO @ Thu, 16 Apr 2020 00:37:14: 22000000 INFO @ Thu, 16 Apr 2020 00:37:14: 15000000 INFO @ Thu, 16 Apr 2020 00:37:20: 21000000 INFO @ Thu, 16 Apr 2020 00:37:23: 23000000 INFO @ Thu, 16 Apr 2020 00:37:24: 16000000 INFO @ Thu, 16 Apr 2020 00:37:29: 22000000 INFO @ Thu, 16 Apr 2020 00:37:31: 24000000 INFO @ Thu, 16 Apr 2020 00:37:33: 17000000 INFO @ Thu, 16 Apr 2020 00:37:37: 23000000 INFO @ Thu, 16 Apr 2020 00:37:40: 25000000 INFO @ Thu, 16 Apr 2020 00:37:41: 18000000 INFO @ Thu, 16 Apr 2020 00:37:46: 24000000 INFO @ Thu, 16 Apr 2020 00:37:49: 26000000 INFO @ Thu, 16 Apr 2020 00:37:50: 19000000 INFO @ Thu, 16 Apr 2020 00:37:55: 25000000 INFO @ Thu, 16 Apr 2020 00:37:57: 27000000 INFO @ Thu, 16 Apr 2020 00:37:59: 20000000 INFO @ Thu, 16 Apr 2020 00:38:03: 26000000 INFO @ Thu, 16 Apr 2020 00:38:06: 28000000 INFO @ Thu, 16 Apr 2020 00:38:08: 21000000 INFO @ Thu, 16 Apr 2020 00:38:12: 27000000 INFO @ Thu, 16 Apr 2020 00:38:15: 29000000 INFO @ Thu, 16 Apr 2020 00:38:16: 22000000 INFO @ Thu, 16 Apr 2020 00:38:21: 28000000 INFO @ Thu, 16 Apr 2020 00:38:24: 30000000 INFO @ Thu, 16 Apr 2020 00:38:25: 23000000 INFO @ Thu, 16 Apr 2020 00:38:29: 29000000 INFO @ Thu, 16 Apr 2020 00:38:32: 31000000 INFO @ Thu, 16 Apr 2020 00:38:35: 24000000 INFO @ Thu, 16 Apr 2020 00:38:39: 30000000 INFO @ Thu, 16 Apr 2020 00:38:42: 32000000 INFO @ Thu, 16 Apr 2020 00:38:43: 25000000 INFO @ Thu, 16 Apr 2020 00:38:48: 31000000 INFO @ Thu, 16 Apr 2020 00:38:52: 33000000 INFO @ Thu, 16 Apr 2020 00:38:52: 26000000 INFO @ Thu, 16 Apr 2020 00:38:58: 32000000 INFO @ Thu, 16 Apr 2020 00:39:01: 34000000 INFO @ Thu, 16 Apr 2020 00:39:02: 27000000 INFO @ Thu, 16 Apr 2020 00:39:07: 33000000 INFO @ Thu, 16 Apr 2020 00:39:11: 35000000 INFO @ Thu, 16 Apr 2020 00:39:12: 28000000 INFO @ Thu, 16 Apr 2020 00:39:16: 34000000 INFO @ Thu, 16 Apr 2020 00:39:19: 36000000 INFO @ Thu, 16 Apr 2020 00:39:21: 29000000 INFO @ Thu, 16 Apr 2020 00:39:25: 35000000 INFO @ Thu, 16 Apr 2020 00:39:28: 37000000 INFO @ Thu, 16 Apr 2020 00:39:30: 30000000 INFO @ Thu, 16 Apr 2020 00:39:33: 36000000 INFO @ Thu, 16 Apr 2020 00:39:37: 38000000 INFO @ Thu, 16 Apr 2020 00:39:39: 31000000 INFO @ Thu, 16 Apr 2020 00:39:42: 37000000 INFO @ Thu, 16 Apr 2020 00:39:45: 39000000 INFO @ Thu, 16 Apr 2020 00:39:48: 32000000 INFO @ Thu, 16 Apr 2020 00:39:50: 38000000 INFO @ Thu, 16 Apr 2020 00:39:54: 40000000 INFO @ Thu, 16 Apr 2020 00:39:57: 33000000 INFO @ Thu, 16 Apr 2020 00:39:58: 39000000 INFO @ Thu, 16 Apr 2020 00:40:03: 41000000 INFO @ Thu, 16 Apr 2020 00:40:06: 34000000 INFO @ Thu, 16 Apr 2020 00:40:06: 40000000 INFO @ Thu, 16 Apr 2020 00:40:11: 42000000 INFO @ Thu, 16 Apr 2020 00:40:15: 41000000 INFO @ Thu, 16 Apr 2020 00:40:15: 35000000 INFO @ Thu, 16 Apr 2020 00:40:20: 43000000 INFO @ Thu, 16 Apr 2020 00:40:23: 42000000 INFO @ Thu, 16 Apr 2020 00:40:24: 36000000 INFO @ Thu, 16 Apr 2020 00:40:28: 44000000 INFO @ Thu, 16 Apr 2020 00:40:31: 43000000 INFO @ Thu, 16 Apr 2020 00:40:33: 37000000 INFO @ Thu, 16 Apr 2020 00:40:36: 45000000 INFO @ Thu, 16 Apr 2020 00:40:40: 44000000 INFO @ Thu, 16 Apr 2020 00:40:41: 38000000 INFO @ Thu, 16 Apr 2020 00:40:45: 46000000 INFO @ Thu, 16 Apr 2020 00:40:48: 45000000 INFO @ Thu, 16 Apr 2020 00:40:51: 39000000 INFO @ Thu, 16 Apr 2020 00:40:54: 47000000 INFO @ Thu, 16 Apr 2020 00:40:56: 46000000 INFO @ Thu, 16 Apr 2020 00:41:01: 40000000 INFO @ Thu, 16 Apr 2020 00:41:02: 48000000 INFO @ Thu, 16 Apr 2020 00:41:05: 47000000 INFO @ Thu, 16 Apr 2020 00:41:10: 41000000 INFO @ Thu, 16 Apr 2020 00:41:11: 49000000 INFO @ Thu, 16 Apr 2020 00:41:13: 48000000 INFO @ Thu, 16 Apr 2020 00:41:16: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:41:16: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:41:16: #1 total tags in treatment: 13709957 INFO @ Thu, 16 Apr 2020 00:41:16: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:41:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:41:16: #1 tags after filtering in treatment: 11698100 INFO @ Thu, 16 Apr 2020 00:41:16: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:41:16: #1 finished! INFO @ Thu, 16 Apr 2020 00:41:16: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:41:16: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:41:17: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:41:17: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:41:17: start model_add_line... INFO @ Thu, 16 Apr 2020 00:41:17: start X-correlation... INFO @ Thu, 16 Apr 2020 00:41:17: end of X-cor INFO @ Thu, 16 Apr 2020 00:41:17: #2 finished! INFO @ Thu, 16 Apr 2020 00:41:17: #2 predicted fragment length is 208 bps INFO @ Thu, 16 Apr 2020 00:41:17: #2 alternative fragment length(s) may be 208 bps INFO @ Thu, 16 Apr 2020 00:41:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05_model.r WARNING @ Thu, 16 Apr 2020 00:41:17: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:41:17: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Thu, 16 Apr 2020 00:41:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:41:17: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:41:17: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:41:20: 42000000 INFO @ Thu, 16 Apr 2020 00:41:21: 49000000 INFO @ Thu, 16 Apr 2020 00:41:26: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:41:26: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:41:26: #1 total tags in treatment: 13709957 INFO @ Thu, 16 Apr 2020 00:41:26: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:41:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:41:27: #1 tags after filtering in treatment: 11698100 INFO @ Thu, 16 Apr 2020 00:41:27: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:41:27: #1 finished! INFO @ Thu, 16 Apr 2020 00:41:27: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:41:27: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:41:28: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:41:28: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:41:28: start model_add_line... INFO @ Thu, 16 Apr 2020 00:41:28: start X-correlation... INFO @ Thu, 16 Apr 2020 00:41:28: end of X-cor INFO @ Thu, 16 Apr 2020 00:41:28: #2 finished! INFO @ Thu, 16 Apr 2020 00:41:28: #2 predicted fragment length is 208 bps INFO @ Thu, 16 Apr 2020 00:41:28: #2 alternative fragment length(s) may be 208 bps INFO @ Thu, 16 Apr 2020 00:41:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10_model.r WARNING @ Thu, 16 Apr 2020 00:41:28: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:41:28: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Thu, 16 Apr 2020 00:41:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:41:28: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:41:28: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:41:28: 43000000 INFO @ Thu, 16 Apr 2020 00:41:37: 44000000 INFO @ Thu, 16 Apr 2020 00:41:40: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:41:46: 45000000 INFO @ Thu, 16 Apr 2020 00:41:50: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:41:52: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:41:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:41:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.05_summits.bed INFO @ Thu, 16 Apr 2020 00:41:52: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (465 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:41:55: 46000000 INFO @ Thu, 16 Apr 2020 00:42:02: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:42:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:42:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.10_summits.bed INFO @ Thu, 16 Apr 2020 00:42:02: Done! pass1 - making usageList (7 chroms): 0 millis pass2 - checking and writing primary data (336 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:42:03: 47000000 INFO @ Thu, 16 Apr 2020 00:42:12: 48000000 INFO @ Thu, 16 Apr 2020 00:42:20: 49000000 INFO @ Thu, 16 Apr 2020 00:42:25: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:42:25: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:42:25: #1 total tags in treatment: 13709957 INFO @ Thu, 16 Apr 2020 00:42:25: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:42:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:42:26: #1 tags after filtering in treatment: 11698100 INFO @ Thu, 16 Apr 2020 00:42:26: #1 Redundant rate of treatment: 0.15 INFO @ Thu, 16 Apr 2020 00:42:26: #1 finished! INFO @ Thu, 16 Apr 2020 00:42:26: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:42:26: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:42:26: #2 number of paired peaks: 279 WARNING @ Thu, 16 Apr 2020 00:42:26: Fewer paired peaks (279) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 279 pairs to build model! INFO @ Thu, 16 Apr 2020 00:42:26: start model_add_line... INFO @ Thu, 16 Apr 2020 00:42:27: start X-correlation... INFO @ Thu, 16 Apr 2020 00:42:27: end of X-cor INFO @ Thu, 16 Apr 2020 00:42:27: #2 finished! INFO @ Thu, 16 Apr 2020 00:42:27: #2 predicted fragment length is 208 bps INFO @ Thu, 16 Apr 2020 00:42:27: #2 alternative fragment length(s) may be 208 bps INFO @ Thu, 16 Apr 2020 00:42:27: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20_model.r WARNING @ Thu, 16 Apr 2020 00:42:27: #2 Since the d (208) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:42:27: #2 You may need to consider one of the other alternative d(s): 208 WARNING @ Thu, 16 Apr 2020 00:42:27: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:42:27: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:42:27: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:42:49: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:43:01: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:43:01: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:43:01: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619547/SRX6619547.20_summits.bed INFO @ Thu, 16 Apr 2020 00:43:01: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (225 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。