Job ID = 5720172 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:48:32 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:56:28 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:12:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:16:03 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:16:05 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:23:58 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T13:26:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 48,643,538 reads read : 97,287,076 reads written : 97,287,076 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:04:31 48643538 reads; of these: 48643538 (100.00%) were paired; of these: 31585283 (64.93%) aligned concordantly 0 times 14097030 (28.98%) aligned concordantly exactly 1 time 2961225 (6.09%) aligned concordantly >1 times ---- 31585283 pairs aligned concordantly 0 times; of these: 6540567 (20.71%) aligned discordantly 1 time ---- 25044716 pairs aligned 0 times concordantly or discordantly; of these: 50089432 mates make up the pairs; of these: 47293964 (94.42%) aligned 0 times 986215 (1.97%) aligned exactly 1 time 1809253 (3.61%) aligned >1 times 51.39% overall alignment rate Time searching: 01:04:31 Overall time: 01:04:31 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3147829 / 23384536 = 0.1346 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:06:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:06:22: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:06:22: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:06:29: 1000000 INFO @ Thu, 16 Apr 2020 00:06:36: 2000000 INFO @ Thu, 16 Apr 2020 00:06:43: 3000000 INFO @ Thu, 16 Apr 2020 00:06:50: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:06:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:06:52: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:06:52: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:06:58: 5000000 INFO @ Thu, 16 Apr 2020 00:06:59: 1000000 INFO @ Thu, 16 Apr 2020 00:07:05: 6000000 INFO @ Thu, 16 Apr 2020 00:07:06: 2000000 INFO @ Thu, 16 Apr 2020 00:07:13: 7000000 INFO @ Thu, 16 Apr 2020 00:07:14: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:07:20: 8000000 INFO @ Thu, 16 Apr 2020 00:07:21: 4000000 INFO @ Thu, 16 Apr 2020 00:07:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:07:22: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:07:22: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:07:28: 9000000 INFO @ Thu, 16 Apr 2020 00:07:29: 5000000 INFO @ Thu, 16 Apr 2020 00:07:30: 1000000 INFO @ Thu, 16 Apr 2020 00:07:36: 10000000 INFO @ Thu, 16 Apr 2020 00:07:37: 6000000 INFO @ Thu, 16 Apr 2020 00:07:38: 2000000 INFO @ Thu, 16 Apr 2020 00:07:44: 11000000 INFO @ Thu, 16 Apr 2020 00:07:45: 7000000 INFO @ Thu, 16 Apr 2020 00:07:46: 3000000 INFO @ Thu, 16 Apr 2020 00:07:53: 12000000 INFO @ Thu, 16 Apr 2020 00:07:53: 8000000 INFO @ Thu, 16 Apr 2020 00:07:54: 4000000 INFO @ Thu, 16 Apr 2020 00:08:00: 13000000 INFO @ Thu, 16 Apr 2020 00:08:01: 9000000 INFO @ Thu, 16 Apr 2020 00:08:02: 5000000 INFO @ Thu, 16 Apr 2020 00:08:09: 10000000 INFO @ Thu, 16 Apr 2020 00:08:09: 14000000 INFO @ Thu, 16 Apr 2020 00:08:10: 6000000 INFO @ Thu, 16 Apr 2020 00:08:16: 11000000 INFO @ Thu, 16 Apr 2020 00:08:17: 15000000 INFO @ Thu, 16 Apr 2020 00:08:17: 7000000 INFO @ Thu, 16 Apr 2020 00:08:24: 12000000 INFO @ Thu, 16 Apr 2020 00:08:24: 16000000 INFO @ Thu, 16 Apr 2020 00:08:25: 8000000 INFO @ Thu, 16 Apr 2020 00:08:32: 13000000 INFO @ Thu, 16 Apr 2020 00:08:32: 17000000 INFO @ Thu, 16 Apr 2020 00:08:33: 9000000 INFO @ Thu, 16 Apr 2020 00:08:39: 14000000 INFO @ Thu, 16 Apr 2020 00:08:40: 18000000 INFO @ Thu, 16 Apr 2020 00:08:40: 10000000 INFO @ Thu, 16 Apr 2020 00:08:47: 15000000 INFO @ Thu, 16 Apr 2020 00:08:47: 19000000 INFO @ Thu, 16 Apr 2020 00:08:48: 11000000 INFO @ Thu, 16 Apr 2020 00:08:54: 16000000 INFO @ Thu, 16 Apr 2020 00:08:55: 20000000 INFO @ Thu, 16 Apr 2020 00:08:56: 12000000 INFO @ Thu, 16 Apr 2020 00:09:02: 17000000 INFO @ Thu, 16 Apr 2020 00:09:02: 21000000 INFO @ Thu, 16 Apr 2020 00:09:03: 13000000 INFO @ Thu, 16 Apr 2020 00:09:10: 22000000 INFO @ Thu, 16 Apr 2020 00:09:10: 18000000 INFO @ Thu, 16 Apr 2020 00:09:11: 14000000 INFO @ Thu, 16 Apr 2020 00:09:17: 23000000 INFO @ Thu, 16 Apr 2020 00:09:17: 19000000 INFO @ Thu, 16 Apr 2020 00:09:18: 15000000 INFO @ Thu, 16 Apr 2020 00:09:24: 24000000 INFO @ Thu, 16 Apr 2020 00:09:25: 20000000 INFO @ Thu, 16 Apr 2020 00:09:26: 16000000 INFO @ Thu, 16 Apr 2020 00:09:31: 25000000 INFO @ Thu, 16 Apr 2020 00:09:32: 21000000 INFO @ Thu, 16 Apr 2020 00:09:34: 17000000 INFO @ Thu, 16 Apr 2020 00:09:39: 26000000 INFO @ Thu, 16 Apr 2020 00:09:40: 22000000 INFO @ Thu, 16 Apr 2020 00:09:41: 18000000 INFO @ Thu, 16 Apr 2020 00:09:46: 27000000 INFO @ Thu, 16 Apr 2020 00:09:48: 23000000 INFO @ Thu, 16 Apr 2020 00:09:49: 19000000 INFO @ Thu, 16 Apr 2020 00:09:53: 28000000 INFO @ Thu, 16 Apr 2020 00:09:55: 24000000 INFO @ Thu, 16 Apr 2020 00:09:56: 20000000 INFO @ Thu, 16 Apr 2020 00:10:01: 29000000 INFO @ Thu, 16 Apr 2020 00:10:03: 25000000 INFO @ Thu, 16 Apr 2020 00:10:04: 21000000 INFO @ Thu, 16 Apr 2020 00:10:08: 30000000 INFO @ Thu, 16 Apr 2020 00:10:10: 26000000 INFO @ Thu, 16 Apr 2020 00:10:11: 22000000 INFO @ Thu, 16 Apr 2020 00:10:16: 31000000 INFO @ Thu, 16 Apr 2020 00:10:17: 27000000 INFO @ Thu, 16 Apr 2020 00:10:19: 23000000 INFO @ Thu, 16 Apr 2020 00:10:23: 32000000 INFO @ Thu, 16 Apr 2020 00:10:25: 28000000 INFO @ Thu, 16 Apr 2020 00:10:26: 24000000 INFO @ Thu, 16 Apr 2020 00:10:31: 33000000 INFO @ Thu, 16 Apr 2020 00:10:32: 29000000 INFO @ Thu, 16 Apr 2020 00:10:33: 25000000 INFO @ Thu, 16 Apr 2020 00:10:38: 34000000 INFO @ Thu, 16 Apr 2020 00:10:39: 30000000 INFO @ Thu, 16 Apr 2020 00:10:41: 26000000 INFO @ Thu, 16 Apr 2020 00:10:45: 35000000 INFO @ Thu, 16 Apr 2020 00:10:47: 31000000 INFO @ Thu, 16 Apr 2020 00:10:48: 27000000 INFO @ Thu, 16 Apr 2020 00:10:53: 36000000 INFO @ Thu, 16 Apr 2020 00:10:54: 32000000 INFO @ Thu, 16 Apr 2020 00:10:56: 28000000 INFO @ Thu, 16 Apr 2020 00:11:00: 37000000 INFO @ Thu, 16 Apr 2020 00:11:01: 33000000 INFO @ Thu, 16 Apr 2020 00:11:03: 29000000 INFO @ Thu, 16 Apr 2020 00:11:07: 38000000 INFO @ Thu, 16 Apr 2020 00:11:09: 34000000 INFO @ Thu, 16 Apr 2020 00:11:10: 30000000 INFO @ Thu, 16 Apr 2020 00:11:15: 39000000 INFO @ Thu, 16 Apr 2020 00:11:16: 35000000 INFO @ Thu, 16 Apr 2020 00:11:18: 31000000 INFO @ Thu, 16 Apr 2020 00:11:22: 40000000 INFO @ Thu, 16 Apr 2020 00:11:23: 36000000 INFO @ Thu, 16 Apr 2020 00:11:25: 32000000 INFO @ Thu, 16 Apr 2020 00:11:30: 41000000 INFO @ Thu, 16 Apr 2020 00:11:30: 37000000 INFO @ Thu, 16 Apr 2020 00:11:32: 33000000 INFO @ Thu, 16 Apr 2020 00:11:37: 42000000 INFO @ Thu, 16 Apr 2020 00:11:38: 38000000 INFO @ Thu, 16 Apr 2020 00:11:39: 34000000 INFO @ Thu, 16 Apr 2020 00:11:45: 43000000 INFO @ Thu, 16 Apr 2020 00:11:45: 39000000 INFO @ Thu, 16 Apr 2020 00:11:47: 35000000 INFO @ Thu, 16 Apr 2020 00:11:50: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:11:50: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:11:50: #1 total tags in treatment: 14679394 INFO @ Thu, 16 Apr 2020 00:11:50: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:11:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:11:50: #1 tags after filtering in treatment: 12624099 INFO @ Thu, 16 Apr 2020 00:11:50: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:11:50: #1 finished! INFO @ Thu, 16 Apr 2020 00:11:50: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:11:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:11:51: #2 number of paired peaks: 385 WARNING @ Thu, 16 Apr 2020 00:11:51: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Thu, 16 Apr 2020 00:11:51: start model_add_line... INFO @ Thu, 16 Apr 2020 00:11:51: start X-correlation... INFO @ Thu, 16 Apr 2020 00:11:51: end of X-cor INFO @ Thu, 16 Apr 2020 00:11:51: #2 finished! INFO @ Thu, 16 Apr 2020 00:11:51: #2 predicted fragment length is 214 bps INFO @ Thu, 16 Apr 2020 00:11:51: #2 alternative fragment length(s) may be 214 bps INFO @ Thu, 16 Apr 2020 00:11:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05_model.r WARNING @ Thu, 16 Apr 2020 00:11:51: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:11:51: #2 You may need to consider one of the other alternative d(s): 214 WARNING @ Thu, 16 Apr 2020 00:11:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:11:51: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:11:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:11:53: 40000000 INFO @ Thu, 16 Apr 2020 00:11:54: 36000000 INFO @ Thu, 16 Apr 2020 00:12:00: 41000000 INFO @ Thu, 16 Apr 2020 00:12:01: 37000000 INFO @ Thu, 16 Apr 2020 00:12:08: 42000000 INFO @ Thu, 16 Apr 2020 00:12:09: 38000000 INFO @ Thu, 16 Apr 2020 00:12:15: 43000000 INFO @ Thu, 16 Apr 2020 00:12:16: 39000000 INFO @ Thu, 16 Apr 2020 00:12:18: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:12:20: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:12:20: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:12:20: #1 total tags in treatment: 14679394 INFO @ Thu, 16 Apr 2020 00:12:20: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:12:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:12:21: #1 tags after filtering in treatment: 12624099 INFO @ Thu, 16 Apr 2020 00:12:21: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:12:21: #1 finished! INFO @ Thu, 16 Apr 2020 00:12:21: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:12:21: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:12:21: #2 number of paired peaks: 385 WARNING @ Thu, 16 Apr 2020 00:12:21: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Thu, 16 Apr 2020 00:12:21: start model_add_line... INFO @ Thu, 16 Apr 2020 00:12:21: start X-correlation... INFO @ Thu, 16 Apr 2020 00:12:21: end of X-cor INFO @ Thu, 16 Apr 2020 00:12:21: #2 finished! INFO @ Thu, 16 Apr 2020 00:12:21: #2 predicted fragment length is 214 bps INFO @ Thu, 16 Apr 2020 00:12:21: #2 alternative fragment length(s) may be 214 bps INFO @ Thu, 16 Apr 2020 00:12:21: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10_model.r WARNING @ Thu, 16 Apr 2020 00:12:21: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:12:21: #2 You may need to consider one of the other alternative d(s): 214 WARNING @ Thu, 16 Apr 2020 00:12:21: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:12:21: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:12:21: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:12:23: 40000000 INFO @ Thu, 16 Apr 2020 00:12:30: 41000000 INFO @ Thu, 16 Apr 2020 00:12:31: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:12:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:12:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.05_summits.bed INFO @ Thu, 16 Apr 2020 00:12:31: Done! pass1 - making usageList (7 chroms): 3 millis pass2 - checking and writing primary data (21265 records, 4 fields): 18 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:12:38: 42000000 INFO @ Thu, 16 Apr 2020 00:12:45: 43000000 INFO @ Thu, 16 Apr 2020 00:12:47: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:12:50: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:12:50: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:12:50: #1 total tags in treatment: 14679394 INFO @ Thu, 16 Apr 2020 00:12:50: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:12:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:12:50: #1 tags after filtering in treatment: 12624099 INFO @ Thu, 16 Apr 2020 00:12:50: #1 Redundant rate of treatment: 0.14 INFO @ Thu, 16 Apr 2020 00:12:50: #1 finished! INFO @ Thu, 16 Apr 2020 00:12:50: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:12:50: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:12:51: #2 number of paired peaks: 385 WARNING @ Thu, 16 Apr 2020 00:12:51: Fewer paired peaks (385) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 385 pairs to build model! INFO @ Thu, 16 Apr 2020 00:12:51: start model_add_line... INFO @ Thu, 16 Apr 2020 00:12:51: start X-correlation... INFO @ Thu, 16 Apr 2020 00:12:51: end of X-cor INFO @ Thu, 16 Apr 2020 00:12:51: #2 finished! INFO @ Thu, 16 Apr 2020 00:12:51: #2 predicted fragment length is 214 bps INFO @ Thu, 16 Apr 2020 00:12:51: #2 alternative fragment length(s) may be 214 bps INFO @ Thu, 16 Apr 2020 00:12:51: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20_model.r WARNING @ Thu, 16 Apr 2020 00:12:51: #2 Since the d (214) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:12:51: #2 You may need to consider one of the other alternative d(s): 214 WARNING @ Thu, 16 Apr 2020 00:12:51: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:12:51: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:12:51: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:13:00: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:13:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:13:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.10_summits.bed INFO @ Thu, 16 Apr 2020 00:13:00: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (8536 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:13:17: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:13:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619543/SRX6619543.20_summits.bed INFO @ Thu, 16 Apr 2020 00:13:29: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (948 records, 4 fields): 2 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。