Job ID = 5720158 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-04-15T12:19:53 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:24:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:24:10 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:25:31 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:26:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:26:24 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:28:29 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:38:33 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:39:34 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:53:45 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2020-04-15T12:58:06 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 29,592,922 reads read : 59,185,844 reads written : 59,185,844 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:25:32 29592922 reads; of these: 29592922 (100.00%) were paired; of these: 14065720 (47.53%) aligned concordantly 0 times 11866853 (40.10%) aligned concordantly exactly 1 time 3660349 (12.37%) aligned concordantly >1 times ---- 14065720 pairs aligned concordantly 0 times; of these: 7315143 (52.01%) aligned discordantly 1 time ---- 6750577 pairs aligned 0 times concordantly or discordantly; of these: 13501154 mates make up the pairs; of these: 10065821 (74.56%) aligned 0 times 1111197 (8.23%) aligned exactly 1 time 2324136 (17.21%) aligned >1 times 82.99% overall alignment rate Time searching: 01:25:32 Overall time: 01:25:33 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 36 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] 3100995 / 22324627 = 0.1389 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:09:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:09:31: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:09:31: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:09:41: 1000000 INFO @ Thu, 16 Apr 2020 00:09:50: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:09:59: 3000000 INFO @ Thu, 16 Apr 2020 00:10:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:10:01: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:10:01: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:10:09: 4000000 INFO @ Thu, 16 Apr 2020 00:10:12: 1000000 INFO @ Thu, 16 Apr 2020 00:10:19: 5000000 INFO @ Thu, 16 Apr 2020 00:10:22: 2000000 INFO @ Thu, 16 Apr 2020 00:10:28: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Thu, 16 Apr 2020 00:10:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 16 Apr 2020 00:10:31: #1 read tag files... INFO @ Thu, 16 Apr 2020 00:10:31: #1 read treatment tags... INFO @ Thu, 16 Apr 2020 00:10:33: 3000000 INFO @ Thu, 16 Apr 2020 00:10:38: 7000000 INFO @ Thu, 16 Apr 2020 00:10:42: 1000000 INFO @ Thu, 16 Apr 2020 00:10:44: 4000000 INFO @ Thu, 16 Apr 2020 00:10:49: 8000000 INFO @ Thu, 16 Apr 2020 00:10:53: 2000000 INFO @ Thu, 16 Apr 2020 00:10:54: 5000000 INFO @ Thu, 16 Apr 2020 00:11:00: 9000000 INFO @ Thu, 16 Apr 2020 00:11:04: 6000000 INFO @ Thu, 16 Apr 2020 00:11:05: 3000000 INFO @ Thu, 16 Apr 2020 00:11:12: 10000000 INFO @ Thu, 16 Apr 2020 00:11:14: 7000000 INFO @ Thu, 16 Apr 2020 00:11:16: 4000000 INFO @ Thu, 16 Apr 2020 00:11:22: 11000000 INFO @ Thu, 16 Apr 2020 00:11:24: 8000000 INFO @ Thu, 16 Apr 2020 00:11:27: 5000000 INFO @ Thu, 16 Apr 2020 00:11:34: 12000000 INFO @ Thu, 16 Apr 2020 00:11:35: 9000000 INFO @ Thu, 16 Apr 2020 00:11:38: 6000000 INFO @ Thu, 16 Apr 2020 00:11:44: 13000000 INFO @ Thu, 16 Apr 2020 00:11:47: 10000000 INFO @ Thu, 16 Apr 2020 00:11:48: 7000000 INFO @ Thu, 16 Apr 2020 00:11:55: 14000000 INFO @ Thu, 16 Apr 2020 00:11:57: 11000000 INFO @ Thu, 16 Apr 2020 00:11:59: 8000000 INFO @ Thu, 16 Apr 2020 00:12:06: 15000000 INFO @ Thu, 16 Apr 2020 00:12:08: 12000000 INFO @ Thu, 16 Apr 2020 00:12:10: 9000000 INFO @ Thu, 16 Apr 2020 00:12:16: 16000000 INFO @ Thu, 16 Apr 2020 00:12:18: 13000000 INFO @ Thu, 16 Apr 2020 00:12:20: 10000000 INFO @ Thu, 16 Apr 2020 00:12:27: 17000000 INFO @ Thu, 16 Apr 2020 00:12:29: 14000000 INFO @ Thu, 16 Apr 2020 00:12:32: 11000000 INFO @ Thu, 16 Apr 2020 00:12:38: 18000000 INFO @ Thu, 16 Apr 2020 00:12:40: 15000000 INFO @ Thu, 16 Apr 2020 00:12:42: 12000000 INFO @ Thu, 16 Apr 2020 00:12:48: 19000000 INFO @ Thu, 16 Apr 2020 00:12:51: 16000000 INFO @ Thu, 16 Apr 2020 00:12:54: 13000000 INFO @ Thu, 16 Apr 2020 00:13:00: 20000000 INFO @ Thu, 16 Apr 2020 00:13:01: 17000000 INFO @ Thu, 16 Apr 2020 00:13:04: 14000000 INFO @ Thu, 16 Apr 2020 00:13:10: 21000000 INFO @ Thu, 16 Apr 2020 00:13:12: 18000000 INFO @ Thu, 16 Apr 2020 00:13:15: 15000000 INFO @ Thu, 16 Apr 2020 00:13:21: 22000000 INFO @ Thu, 16 Apr 2020 00:13:23: 19000000 INFO @ Thu, 16 Apr 2020 00:13:26: 16000000 INFO @ Thu, 16 Apr 2020 00:13:32: 23000000 INFO @ Thu, 16 Apr 2020 00:13:33: 20000000 INFO @ Thu, 16 Apr 2020 00:13:37: 17000000 INFO @ Thu, 16 Apr 2020 00:13:43: 21000000 INFO @ Thu, 16 Apr 2020 00:13:43: 24000000 INFO @ Thu, 16 Apr 2020 00:13:48: 18000000 INFO @ Thu, 16 Apr 2020 00:13:54: 22000000 INFO @ Thu, 16 Apr 2020 00:13:54: 25000000 INFO @ Thu, 16 Apr 2020 00:13:59: 19000000 INFO @ Thu, 16 Apr 2020 00:14:04: 23000000 INFO @ Thu, 16 Apr 2020 00:14:05: 26000000 INFO @ Thu, 16 Apr 2020 00:14:09: 20000000 INFO @ Thu, 16 Apr 2020 00:14:16: 24000000 INFO @ Thu, 16 Apr 2020 00:14:16: 27000000 INFO @ Thu, 16 Apr 2020 00:14:21: 21000000 INFO @ Thu, 16 Apr 2020 00:14:27: 25000000 INFO @ Thu, 16 Apr 2020 00:14:27: 28000000 INFO @ Thu, 16 Apr 2020 00:14:31: 22000000 INFO @ Thu, 16 Apr 2020 00:14:37: 29000000 INFO @ Thu, 16 Apr 2020 00:14:37: 26000000 INFO @ Thu, 16 Apr 2020 00:14:42: 23000000 INFO @ Thu, 16 Apr 2020 00:14:48: 30000000 INFO @ Thu, 16 Apr 2020 00:14:49: 27000000 INFO @ Thu, 16 Apr 2020 00:14:53: 24000000 INFO @ Thu, 16 Apr 2020 00:14:58: 31000000 INFO @ Thu, 16 Apr 2020 00:14:59: 28000000 INFO @ Thu, 16 Apr 2020 00:15:04: 25000000 INFO @ Thu, 16 Apr 2020 00:15:09: 32000000 INFO @ Thu, 16 Apr 2020 00:15:10: 29000000 INFO @ Thu, 16 Apr 2020 00:15:15: 26000000 INFO @ Thu, 16 Apr 2020 00:15:19: 33000000 INFO @ Thu, 16 Apr 2020 00:15:21: 30000000 INFO @ Thu, 16 Apr 2020 00:15:25: 27000000 INFO @ Thu, 16 Apr 2020 00:15:30: 34000000 INFO @ Thu, 16 Apr 2020 00:15:31: 31000000 INFO @ Thu, 16 Apr 2020 00:15:36: 28000000 INFO @ Thu, 16 Apr 2020 00:15:41: 35000000 INFO @ Thu, 16 Apr 2020 00:15:41: 32000000 INFO @ Thu, 16 Apr 2020 00:15:47: 29000000 INFO @ Thu, 16 Apr 2020 00:15:51: 33000000 INFO @ Thu, 16 Apr 2020 00:15:51: 36000000 INFO @ Thu, 16 Apr 2020 00:15:58: 30000000 INFO @ Thu, 16 Apr 2020 00:16:01: 34000000 INFO @ Thu, 16 Apr 2020 00:16:02: 37000000 INFO @ Thu, 16 Apr 2020 00:16:08: 31000000 INFO @ Thu, 16 Apr 2020 00:16:12: 35000000 INFO @ Thu, 16 Apr 2020 00:16:12: 38000000 INFO @ Thu, 16 Apr 2020 00:16:19: 32000000 INFO @ Thu, 16 Apr 2020 00:16:22: 36000000 INFO @ Thu, 16 Apr 2020 00:16:23: 39000000 INFO @ Thu, 16 Apr 2020 00:16:30: 33000000 INFO @ Thu, 16 Apr 2020 00:16:32: 37000000 INFO @ Thu, 16 Apr 2020 00:16:34: 40000000 INFO @ Thu, 16 Apr 2020 00:16:41: 34000000 INFO @ Thu, 16 Apr 2020 00:16:44: 38000000 INFO @ Thu, 16 Apr 2020 00:16:45: 41000000 INFO @ Thu, 16 Apr 2020 00:16:51: 35000000 INFO @ Thu, 16 Apr 2020 00:16:54: 39000000 INFO @ Thu, 16 Apr 2020 00:16:56: 42000000 INFO @ Thu, 16 Apr 2020 00:17:02: 36000000 INFO @ Thu, 16 Apr 2020 00:17:05: 40000000 INFO @ Thu, 16 Apr 2020 00:17:06: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:17:06: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:17:06: #1 total tags in treatment: 13160122 INFO @ Thu, 16 Apr 2020 00:17:06: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:17:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:17:06: #1 tags after filtering in treatment: 10631857 INFO @ Thu, 16 Apr 2020 00:17:06: #1 Redundant rate of treatment: 0.19 INFO @ Thu, 16 Apr 2020 00:17:06: #1 finished! INFO @ Thu, 16 Apr 2020 00:17:06: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:17:07: #2 number of paired peaks: 382 WARNING @ Thu, 16 Apr 2020 00:17:07: Fewer paired peaks (382) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 382 pairs to build model! INFO @ Thu, 16 Apr 2020 00:17:07: start model_add_line... INFO @ Thu, 16 Apr 2020 00:17:07: start X-correlation... INFO @ Thu, 16 Apr 2020 00:17:07: end of X-cor INFO @ Thu, 16 Apr 2020 00:17:07: #2 finished! INFO @ Thu, 16 Apr 2020 00:17:07: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:17:07: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:17:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05_model.r WARNING @ Thu, 16 Apr 2020 00:17:07: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:17:07: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:17:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:17:07: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:17:07: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:17:12: 37000000 INFO @ Thu, 16 Apr 2020 00:17:16: 41000000 INFO @ Thu, 16 Apr 2020 00:17:22: 38000000 INFO @ Thu, 16 Apr 2020 00:17:26: 42000000 INFO @ Thu, 16 Apr 2020 00:17:30: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:17:32: 39000000 INFO @ Thu, 16 Apr 2020 00:17:36: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:17:36: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:17:36: #1 total tags in treatment: 13160122 INFO @ Thu, 16 Apr 2020 00:17:36: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:17:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:17:36: #1 tags after filtering in treatment: 10631857 INFO @ Thu, 16 Apr 2020 00:17:36: #1 Redundant rate of treatment: 0.19 INFO @ Thu, 16 Apr 2020 00:17:36: #1 finished! INFO @ Thu, 16 Apr 2020 00:17:36: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:17:36: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:17:37: #2 number of paired peaks: 382 WARNING @ Thu, 16 Apr 2020 00:17:37: Fewer paired peaks (382) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 382 pairs to build model! INFO @ Thu, 16 Apr 2020 00:17:37: start model_add_line... INFO @ Thu, 16 Apr 2020 00:17:37: start X-correlation... INFO @ Thu, 16 Apr 2020 00:17:37: end of X-cor INFO @ Thu, 16 Apr 2020 00:17:37: #2 finished! INFO @ Thu, 16 Apr 2020 00:17:37: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:17:37: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:17:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10_model.r WARNING @ Thu, 16 Apr 2020 00:17:37: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:17:37: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:17:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:17:37: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:17:37: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:17:42: 40000000 INFO @ Thu, 16 Apr 2020 00:17:43: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05_peaks.xls INFO @ Thu, 16 Apr 2020 00:17:43: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:17:43: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.05_summits.bed INFO @ Thu, 16 Apr 2020 00:17:43: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (504 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:17:52: 41000000 INFO @ Thu, 16 Apr 2020 00:18:00: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:18:01: 42000000 INFO @ Thu, 16 Apr 2020 00:18:09: #1 tag size is determined as 150 bps INFO @ Thu, 16 Apr 2020 00:18:09: #1 tag size = 150 INFO @ Thu, 16 Apr 2020 00:18:09: #1 total tags in treatment: 13160122 INFO @ Thu, 16 Apr 2020 00:18:09: #1 user defined the maximum tags... INFO @ Thu, 16 Apr 2020 00:18:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 16 Apr 2020 00:18:09: #1 tags after filtering in treatment: 10631857 INFO @ Thu, 16 Apr 2020 00:18:09: #1 Redundant rate of treatment: 0.19 INFO @ Thu, 16 Apr 2020 00:18:09: #1 finished! INFO @ Thu, 16 Apr 2020 00:18:09: #2 Build Peak Model... INFO @ Thu, 16 Apr 2020 00:18:09: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 16 Apr 2020 00:18:10: #2 number of paired peaks: 382 WARNING @ Thu, 16 Apr 2020 00:18:10: Fewer paired peaks (382) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 382 pairs to build model! INFO @ Thu, 16 Apr 2020 00:18:10: start model_add_line... INFO @ Thu, 16 Apr 2020 00:18:10: start X-correlation... INFO @ Thu, 16 Apr 2020 00:18:10: end of X-cor INFO @ Thu, 16 Apr 2020 00:18:10: #2 finished! INFO @ Thu, 16 Apr 2020 00:18:10: #2 predicted fragment length is 212 bps INFO @ Thu, 16 Apr 2020 00:18:10: #2 alternative fragment length(s) may be 212 bps INFO @ Thu, 16 Apr 2020 00:18:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20_model.r WARNING @ Thu, 16 Apr 2020 00:18:10: #2 Since the d (212) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Thu, 16 Apr 2020 00:18:10: #2 You may need to consider one of the other alternative d(s): 212 WARNING @ Thu, 16 Apr 2020 00:18:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Thu, 16 Apr 2020 00:18:10: #3 Call peaks... INFO @ Thu, 16 Apr 2020 00:18:10: #3 Pre-compute pvalue-qvalue table... INFO @ Thu, 16 Apr 2020 00:18:12: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10_peaks.xls INFO @ Thu, 16 Apr 2020 00:18:12: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:18:12: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.10_summits.bed INFO @ Thu, 16 Apr 2020 00:18:12: Done! pass1 - making usageList (6 chroms): 0 millis pass2 - checking and writing primary data (334 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Thu, 16 Apr 2020 00:18:33: #3 Call peaks for each chromosome... INFO @ Thu, 16 Apr 2020 00:18:45: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20_peaks.xls INFO @ Thu, 16 Apr 2020 00:18:45: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20_peaks.narrowPeak INFO @ Thu, 16 Apr 2020 00:18:45: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX6619530/SRX6619530.20_summits.bed INFO @ Thu, 16 Apr 2020 00:18:45: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (196 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。