Job ID = 1293224 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 34,727,185 reads read : 34,727,185 reads written : 34,727,185 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:21 34727185 reads; of these: 34727185 (100.00%) were unpaired; of these: 2264876 (6.52%) aligned 0 times 26995426 (77.74%) aligned exactly 1 time 5466883 (15.74%) aligned >1 times 93.48% overall alignment rate Time searching: 00:08:21 Overall time: 00:08:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4736903 / 32462309 = 0.1459 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:57:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:57:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:57:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:57:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:57:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:57:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:57:56: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:57:56: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:57:56: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:58:03: 1000000 INFO @ Sun, 02 Jun 2019 22:58:04: 1000000 INFO @ Sun, 02 Jun 2019 22:58:04: 1000000 INFO @ Sun, 02 Jun 2019 22:58:10: 2000000 INFO @ Sun, 02 Jun 2019 22:58:12: 2000000 INFO @ Sun, 02 Jun 2019 22:58:12: 2000000 INFO @ Sun, 02 Jun 2019 22:58:17: 3000000 INFO @ Sun, 02 Jun 2019 22:58:20: 3000000 INFO @ Sun, 02 Jun 2019 22:58:21: 3000000 INFO @ Sun, 02 Jun 2019 22:58:25: 4000000 INFO @ Sun, 02 Jun 2019 22:58:28: 4000000 INFO @ Sun, 02 Jun 2019 22:58:29: 4000000 INFO @ Sun, 02 Jun 2019 22:58:32: 5000000 INFO @ Sun, 02 Jun 2019 22:58:36: 5000000 INFO @ Sun, 02 Jun 2019 22:58:37: 5000000 INFO @ Sun, 02 Jun 2019 22:58:39: 6000000 INFO @ Sun, 02 Jun 2019 22:58:43: 6000000 INFO @ Sun, 02 Jun 2019 22:58:44: 6000000 INFO @ Sun, 02 Jun 2019 22:58:46: 7000000 INFO @ Sun, 02 Jun 2019 22:58:51: 7000000 INFO @ Sun, 02 Jun 2019 22:58:52: 7000000 INFO @ Sun, 02 Jun 2019 22:58:53: 8000000 INFO @ Sun, 02 Jun 2019 22:58:59: 8000000 INFO @ Sun, 02 Jun 2019 22:59:00: 8000000 INFO @ Sun, 02 Jun 2019 22:59:00: 9000000 INFO @ Sun, 02 Jun 2019 22:59:06: 9000000 INFO @ Sun, 02 Jun 2019 22:59:07: 10000000 INFO @ Sun, 02 Jun 2019 22:59:08: 9000000 INFO @ Sun, 02 Jun 2019 22:59:14: 10000000 INFO @ Sun, 02 Jun 2019 22:59:14: 11000000 INFO @ Sun, 02 Jun 2019 22:59:15: 10000000 INFO @ Sun, 02 Jun 2019 22:59:21: 12000000 INFO @ Sun, 02 Jun 2019 22:59:21: 11000000 INFO @ Sun, 02 Jun 2019 22:59:23: 11000000 INFO @ Sun, 02 Jun 2019 22:59:28: 13000000 INFO @ Sun, 02 Jun 2019 22:59:29: 12000000 INFO @ Sun, 02 Jun 2019 22:59:31: 12000000 INFO @ Sun, 02 Jun 2019 22:59:35: 14000000 INFO @ Sun, 02 Jun 2019 22:59:36: 13000000 INFO @ Sun, 02 Jun 2019 22:59:39: 13000000 INFO @ Sun, 02 Jun 2019 22:59:42: 15000000 INFO @ Sun, 02 Jun 2019 22:59:44: 14000000 INFO @ Sun, 02 Jun 2019 22:59:46: 14000000 INFO @ Sun, 02 Jun 2019 22:59:49: 16000000 INFO @ Sun, 02 Jun 2019 22:59:51: 15000000 INFO @ Sun, 02 Jun 2019 22:59:54: 15000000 INFO @ Sun, 02 Jun 2019 22:59:56: 17000000 INFO @ Sun, 02 Jun 2019 22:59:59: 16000000 INFO @ Sun, 02 Jun 2019 23:00:01: 16000000 INFO @ Sun, 02 Jun 2019 23:00:03: 18000000 INFO @ Sun, 02 Jun 2019 23:00:07: 17000000 INFO @ Sun, 02 Jun 2019 23:00:09: 17000000 INFO @ Sun, 02 Jun 2019 23:00:11: 19000000 INFO @ Sun, 02 Jun 2019 23:00:14: 18000000 INFO @ Sun, 02 Jun 2019 23:00:17: 18000000 INFO @ Sun, 02 Jun 2019 23:00:18: 20000000 INFO @ Sun, 02 Jun 2019 23:00:22: 19000000 INFO @ Sun, 02 Jun 2019 23:00:25: 21000000 INFO @ Sun, 02 Jun 2019 23:00:26: 19000000 INFO @ Sun, 02 Jun 2019 23:00:30: 20000000 INFO @ Sun, 02 Jun 2019 23:00:32: 22000000 INFO @ Sun, 02 Jun 2019 23:00:34: 20000000 INFO @ Sun, 02 Jun 2019 23:00:37: 21000000 INFO @ Sun, 02 Jun 2019 23:00:39: 23000000 INFO @ Sun, 02 Jun 2019 23:00:41: 21000000 INFO @ Sun, 02 Jun 2019 23:00:45: 22000000 INFO @ Sun, 02 Jun 2019 23:00:46: 24000000 INFO @ Sun, 02 Jun 2019 23:00:49: 22000000 INFO @ Sun, 02 Jun 2019 23:00:52: 23000000 INFO @ Sun, 02 Jun 2019 23:00:53: 25000000 INFO @ Sun, 02 Jun 2019 23:00:57: 23000000 INFO @ Sun, 02 Jun 2019 23:01:00: 26000000 INFO @ Sun, 02 Jun 2019 23:01:00: 24000000 INFO @ Sun, 02 Jun 2019 23:01:04: 24000000 INFO @ Sun, 02 Jun 2019 23:01:07: 27000000 INFO @ Sun, 02 Jun 2019 23:01:07: 25000000 INFO @ Sun, 02 Jun 2019 23:01:12: 25000000 INFO @ Sun, 02 Jun 2019 23:01:12: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:01:12: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:01:12: #1 total tags in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:01:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:01:13: #1 tags after filtering in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:13: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:01:13: #1 finished! INFO @ Sun, 02 Jun 2019 23:01:13: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:01:13: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:01:15: 26000000 INFO @ Sun, 02 Jun 2019 23:01:15: #2 number of paired peaks: 87 WARNING @ Sun, 02 Jun 2019 23:01:15: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:01:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:01:19: 26000000 INFO @ Sun, 02 Jun 2019 23:01:22: 27000000 INFO @ Sun, 02 Jun 2019 23:01:27: 27000000 INFO @ Sun, 02 Jun 2019 23:01:28: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:01:28: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:01:28: #1 total tags in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:28: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:01:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:01:28: #1 tags after filtering in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:28: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:01:28: #1 finished! INFO @ Sun, 02 Jun 2019 23:01:28: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:01:28: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:01:31: #2 number of paired peaks: 87 WARNING @ Sun, 02 Jun 2019 23:01:31: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:01:31: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:01:33: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 23:01:33: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 23:01:33: #1 total tags in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:33: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:01:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:01:33: #1 tags after filtering in treatment: 27725406 INFO @ Sun, 02 Jun 2019 23:01:33: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:01:33: #1 finished! INFO @ Sun, 02 Jun 2019 23:01:33: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:01:33: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:01:35: #2 number of paired peaks: 87 WARNING @ Sun, 02 Jun 2019 23:01:35: Too few paired peaks (87) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 23:01:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402773/SRX5402773.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。