Job ID = 1293222 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 41,576,290 reads read : 83,152,580 reads written : 41,576,290 reads 0-length : 41,576,290 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:29 41576290 reads; of these: 41576290 (100.00%) were unpaired; of these: 181708 (0.44%) aligned 0 times 34409340 (82.76%) aligned exactly 1 time 6985242 (16.80%) aligned >1 times 99.56% overall alignment rate Time searching: 00:10:29 Overall time: 00:10:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 20 files... [bam_rmdupse_core] 8682272 / 41394582 = 0.2097 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:51:24: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:51:24: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:51:24: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:51:24: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:51:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:51:24: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:51:24: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:51:32: 1000000 INFO @ Sun, 02 Jun 2019 22:51:33: 1000000 INFO @ Sun, 02 Jun 2019 22:51:34: 1000000 INFO @ Sun, 02 Jun 2019 22:51:39: 2000000 INFO @ Sun, 02 Jun 2019 22:51:41: 2000000 INFO @ Sun, 02 Jun 2019 22:51:44: 2000000 INFO @ Sun, 02 Jun 2019 22:51:46: 3000000 INFO @ Sun, 02 Jun 2019 22:51:49: 3000000 INFO @ Sun, 02 Jun 2019 22:51:53: 3000000 INFO @ Sun, 02 Jun 2019 22:51:54: 4000000 INFO @ Sun, 02 Jun 2019 22:51:57: 4000000 INFO @ Sun, 02 Jun 2019 22:52:01: 5000000 INFO @ Sun, 02 Jun 2019 22:52:03: 4000000 INFO @ Sun, 02 Jun 2019 22:52:06: 5000000 INFO @ Sun, 02 Jun 2019 22:52:09: 6000000 INFO @ Sun, 02 Jun 2019 22:52:12: 5000000 INFO @ Sun, 02 Jun 2019 22:52:15: 6000000 INFO @ Sun, 02 Jun 2019 22:52:17: 7000000 INFO @ Sun, 02 Jun 2019 22:52:22: 6000000 INFO @ Sun, 02 Jun 2019 22:52:23: 7000000 INFO @ Sun, 02 Jun 2019 22:52:24: 8000000 INFO @ Sun, 02 Jun 2019 22:52:31: 7000000 INFO @ Sun, 02 Jun 2019 22:52:32: 8000000 INFO @ Sun, 02 Jun 2019 22:52:32: 9000000 INFO @ Sun, 02 Jun 2019 22:52:40: 10000000 INFO @ Sun, 02 Jun 2019 22:52:40: 9000000 INFO @ Sun, 02 Jun 2019 22:52:41: 8000000 INFO @ Sun, 02 Jun 2019 22:52:47: 11000000 INFO @ Sun, 02 Jun 2019 22:52:48: 10000000 INFO @ Sun, 02 Jun 2019 22:52:50: 9000000 INFO @ Sun, 02 Jun 2019 22:52:54: 12000000 INFO @ Sun, 02 Jun 2019 22:52:56: 11000000 INFO @ Sun, 02 Jun 2019 22:52:59: 10000000 INFO @ Sun, 02 Jun 2019 22:53:02: 13000000 INFO @ Sun, 02 Jun 2019 22:53:04: 12000000 INFO @ Sun, 02 Jun 2019 22:53:09: 11000000 INFO @ Sun, 02 Jun 2019 22:53:09: 14000000 INFO @ Sun, 02 Jun 2019 22:53:12: 13000000 INFO @ Sun, 02 Jun 2019 22:53:16: 15000000 INFO @ Sun, 02 Jun 2019 22:53:18: 12000000 INFO @ Sun, 02 Jun 2019 22:53:20: 14000000 INFO @ Sun, 02 Jun 2019 22:53:23: 16000000 INFO @ Sun, 02 Jun 2019 22:53:27: 13000000 INFO @ Sun, 02 Jun 2019 22:53:28: 15000000 INFO @ Sun, 02 Jun 2019 22:53:31: 17000000 INFO @ Sun, 02 Jun 2019 22:53:36: 16000000 INFO @ Sun, 02 Jun 2019 22:53:37: 14000000 INFO @ Sun, 02 Jun 2019 22:53:38: 18000000 INFO @ Sun, 02 Jun 2019 22:53:44: 17000000 INFO @ Sun, 02 Jun 2019 22:53:45: 19000000 INFO @ Sun, 02 Jun 2019 22:53:46: 15000000 INFO @ Sun, 02 Jun 2019 22:53:52: 18000000 INFO @ Sun, 02 Jun 2019 22:53:52: 20000000 INFO @ Sun, 02 Jun 2019 22:53:56: 16000000 INFO @ Sun, 02 Jun 2019 22:53:59: 21000000 INFO @ Sun, 02 Jun 2019 22:54:00: 19000000 INFO @ Sun, 02 Jun 2019 22:54:05: 17000000 INFO @ Sun, 02 Jun 2019 22:54:06: 22000000 INFO @ Sun, 02 Jun 2019 22:54:08: 20000000 INFO @ Sun, 02 Jun 2019 22:54:13: 23000000 INFO @ Sun, 02 Jun 2019 22:54:14: 18000000 INFO @ Sun, 02 Jun 2019 22:54:16: 21000000 INFO @ Sun, 02 Jun 2019 22:54:20: 24000000 INFO @ Sun, 02 Jun 2019 22:54:23: 19000000 INFO @ Sun, 02 Jun 2019 22:54:24: 22000000 INFO @ Sun, 02 Jun 2019 22:54:27: 25000000 INFO @ Sun, 02 Jun 2019 22:54:32: 23000000 INFO @ Sun, 02 Jun 2019 22:54:33: 20000000 INFO @ Sun, 02 Jun 2019 22:54:34: 26000000 INFO @ Sun, 02 Jun 2019 22:54:40: 24000000 INFO @ Sun, 02 Jun 2019 22:54:41: 27000000 INFO @ Sun, 02 Jun 2019 22:54:42: 21000000 INFO @ Sun, 02 Jun 2019 22:54:48: 25000000 INFO @ Sun, 02 Jun 2019 22:54:48: 28000000 INFO @ Sun, 02 Jun 2019 22:54:52: 22000000 INFO @ Sun, 02 Jun 2019 22:54:55: 29000000 INFO @ Sun, 02 Jun 2019 22:54:56: 26000000 INFO @ Sun, 02 Jun 2019 22:55:01: 23000000 INFO @ Sun, 02 Jun 2019 22:55:02: 30000000 INFO @ Sun, 02 Jun 2019 22:55:04: 27000000 INFO @ Sun, 02 Jun 2019 22:55:09: 31000000 INFO @ Sun, 02 Jun 2019 22:55:11: 24000000 INFO @ Sun, 02 Jun 2019 22:55:12: 28000000 INFO @ Sun, 02 Jun 2019 22:55:16: 32000000 INFO @ Sun, 02 Jun 2019 22:55:20: 29000000 INFO @ Sun, 02 Jun 2019 22:55:20: 25000000 INFO @ Sun, 02 Jun 2019 22:55:21: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 22:55:21: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 22:55:21: #1 total tags in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:55:21: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:55:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:55:22: #1 tags after filtering in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:55:22: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:55:22: #1 finished! INFO @ Sun, 02 Jun 2019 22:55:22: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:55:22: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:55:25: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:55:25: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:55:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:55:28: 30000000 INFO @ Sun, 02 Jun 2019 22:55:29: 26000000 INFO @ Sun, 02 Jun 2019 22:55:36: 31000000 INFO @ Sun, 02 Jun 2019 22:55:38: 27000000 INFO @ Sun, 02 Jun 2019 22:55:44: 32000000 INFO @ Sun, 02 Jun 2019 22:55:48: 28000000 INFO @ Sun, 02 Jun 2019 22:55:50: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 22:55:50: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 22:55:50: #1 total tags in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:55:50: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:55:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:55:50: #1 tags after filtering in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:55:50: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:55:50: #1 finished! INFO @ Sun, 02 Jun 2019 22:55:50: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:55:50: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:55:53: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:55:53: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:55:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:55:57: 29000000 INFO @ Sun, 02 Jun 2019 22:56:06: 30000000 INFO @ Sun, 02 Jun 2019 22:56:15: 31000000 INFO @ Sun, 02 Jun 2019 22:56:25: 32000000 INFO @ Sun, 02 Jun 2019 22:56:32: #1 tag size is determined as 51 bps INFO @ Sun, 02 Jun 2019 22:56:32: #1 tag size = 51 INFO @ Sun, 02 Jun 2019 22:56:32: #1 total tags in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:56:32: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:56:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:56:32: #1 tags after filtering in treatment: 32712310 INFO @ Sun, 02 Jun 2019 22:56:32: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:56:32: #1 finished! INFO @ Sun, 02 Jun 2019 22:56:32: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:56:32: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:56:35: #2 number of paired peaks: 32 WARNING @ Sun, 02 Jun 2019 22:56:35: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:56:35: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402771/SRX5402771.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。