Job ID = 1293134 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 42,455,837 reads read : 42,455,837 reads written : 42,455,837 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:40 42455837 reads; of these: 42455837 (100.00%) were unpaired; of these: 3744300 (8.82%) aligned 0 times 33541340 (79.00%) aligned exactly 1 time 5170197 (12.18%) aligned >1 times 91.18% overall alignment rate Time searching: 00:09:40 Overall time: 00:09:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 10353844 / 38711537 = 0.2675 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 22:39:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:39:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:39:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:39:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:39:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:39:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:39:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 22:39:26: #1 read tag files... INFO @ Sun, 02 Jun 2019 22:39:26: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 22:39:35: 1000000 INFO @ Sun, 02 Jun 2019 22:39:35: 1000000 INFO @ Sun, 02 Jun 2019 22:39:36: 1000000 INFO @ Sun, 02 Jun 2019 22:39:42: 2000000 INFO @ Sun, 02 Jun 2019 22:39:43: 2000000 INFO @ Sun, 02 Jun 2019 22:39:46: 2000000 INFO @ Sun, 02 Jun 2019 22:39:50: 3000000 INFO @ Sun, 02 Jun 2019 22:39:52: 3000000 INFO @ Sun, 02 Jun 2019 22:39:55: 3000000 INFO @ Sun, 02 Jun 2019 22:39:57: 4000000 INFO @ Sun, 02 Jun 2019 22:40:00: 4000000 INFO @ Sun, 02 Jun 2019 22:40:05: 5000000 INFO @ Sun, 02 Jun 2019 22:40:05: 4000000 INFO @ Sun, 02 Jun 2019 22:40:08: 5000000 INFO @ Sun, 02 Jun 2019 22:40:12: 6000000 INFO @ Sun, 02 Jun 2019 22:40:15: 5000000 INFO @ Sun, 02 Jun 2019 22:40:16: 6000000 INFO @ Sun, 02 Jun 2019 22:40:19: 7000000 INFO @ Sun, 02 Jun 2019 22:40:24: 6000000 INFO @ Sun, 02 Jun 2019 22:40:24: 7000000 INFO @ Sun, 02 Jun 2019 22:40:27: 8000000 INFO @ Sun, 02 Jun 2019 22:40:32: 8000000 INFO @ Sun, 02 Jun 2019 22:40:33: 7000000 INFO @ Sun, 02 Jun 2019 22:40:34: 9000000 INFO @ Sun, 02 Jun 2019 22:40:40: 9000000 INFO @ Sun, 02 Jun 2019 22:40:41: 10000000 INFO @ Sun, 02 Jun 2019 22:40:43: 8000000 INFO @ Sun, 02 Jun 2019 22:40:48: 10000000 INFO @ Sun, 02 Jun 2019 22:40:49: 11000000 INFO @ Sun, 02 Jun 2019 22:40:52: 9000000 INFO @ Sun, 02 Jun 2019 22:40:56: 11000000 INFO @ Sun, 02 Jun 2019 22:40:56: 12000000 INFO @ Sun, 02 Jun 2019 22:41:01: 10000000 INFO @ Sun, 02 Jun 2019 22:41:04: 12000000 INFO @ Sun, 02 Jun 2019 22:41:04: 13000000 INFO @ Sun, 02 Jun 2019 22:41:11: 11000000 INFO @ Sun, 02 Jun 2019 22:41:12: 13000000 INFO @ Sun, 02 Jun 2019 22:41:12: 14000000 INFO @ Sun, 02 Jun 2019 22:41:20: 14000000 INFO @ Sun, 02 Jun 2019 22:41:20: 15000000 INFO @ Sun, 02 Jun 2019 22:41:20: 12000000 INFO @ Sun, 02 Jun 2019 22:41:28: 16000000 INFO @ Sun, 02 Jun 2019 22:41:28: 15000000 INFO @ Sun, 02 Jun 2019 22:41:30: 13000000 INFO @ Sun, 02 Jun 2019 22:41:36: 17000000 INFO @ Sun, 02 Jun 2019 22:41:36: 16000000 INFO @ Sun, 02 Jun 2019 22:41:39: 14000000 INFO @ Sun, 02 Jun 2019 22:41:44: 18000000 INFO @ Sun, 02 Jun 2019 22:41:44: 17000000 INFO @ Sun, 02 Jun 2019 22:41:49: 15000000 INFO @ Sun, 02 Jun 2019 22:41:51: 19000000 INFO @ Sun, 02 Jun 2019 22:41:52: 18000000 INFO @ Sun, 02 Jun 2019 22:41:59: 16000000 INFO @ Sun, 02 Jun 2019 22:41:59: 20000000 INFO @ Sun, 02 Jun 2019 22:42:00: 19000000 INFO @ Sun, 02 Jun 2019 22:42:06: 21000000 INFO @ Sun, 02 Jun 2019 22:42:08: 17000000 INFO @ Sun, 02 Jun 2019 22:42:08: 20000000 INFO @ Sun, 02 Jun 2019 22:42:13: 22000000 INFO @ Sun, 02 Jun 2019 22:42:16: 21000000 INFO @ Sun, 02 Jun 2019 22:42:17: 18000000 INFO @ Sun, 02 Jun 2019 22:42:21: 23000000 INFO @ Sun, 02 Jun 2019 22:42:24: 22000000 INFO @ Sun, 02 Jun 2019 22:42:27: 19000000 INFO @ Sun, 02 Jun 2019 22:42:28: 24000000 INFO @ Sun, 02 Jun 2019 22:42:32: 23000000 INFO @ Sun, 02 Jun 2019 22:42:35: 25000000 INFO @ Sun, 02 Jun 2019 22:42:36: 20000000 INFO @ Sun, 02 Jun 2019 22:42:40: 24000000 INFO @ Sun, 02 Jun 2019 22:42:42: 26000000 INFO @ Sun, 02 Jun 2019 22:42:45: 21000000 INFO @ Sun, 02 Jun 2019 22:42:48: 25000000 INFO @ Sun, 02 Jun 2019 22:42:50: 27000000 INFO @ Sun, 02 Jun 2019 22:42:54: 22000000 INFO @ Sun, 02 Jun 2019 22:42:55: 26000000 INFO @ Sun, 02 Jun 2019 22:42:57: 28000000 INFO @ Sun, 02 Jun 2019 22:43:00: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:43:00: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:43:00: #1 total tags in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:00: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:43:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:43:00: #1 tags after filtering in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:00: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:43:00: #1 finished! INFO @ Sun, 02 Jun 2019 22:43:00: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:43:00: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:43:03: #2 number of paired peaks: 59 WARNING @ Sun, 02 Jun 2019 22:43:03: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:43:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:43:04: 23000000 INFO @ Sun, 02 Jun 2019 22:43:04: 27000000 INFO @ Sun, 02 Jun 2019 22:43:12: 28000000 INFO @ Sun, 02 Jun 2019 22:43:13: 24000000 INFO @ Sun, 02 Jun 2019 22:43:15: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:43:15: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:43:15: #1 total tags in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:15: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:43:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:43:15: #1 tags after filtering in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:15: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:43:15: #1 finished! INFO @ Sun, 02 Jun 2019 22:43:15: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:43:15: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:43:18: #2 number of paired peaks: 59 WARNING @ Sun, 02 Jun 2019 22:43:18: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:43:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 22:43:22: 25000000 INFO @ Sun, 02 Jun 2019 22:43:31: 26000000 INFO @ Sun, 02 Jun 2019 22:43:40: 27000000 INFO @ Sun, 02 Jun 2019 22:43:49: 28000000 INFO @ Sun, 02 Jun 2019 22:43:53: #1 tag size is determined as 50 bps INFO @ Sun, 02 Jun 2019 22:43:53: #1 tag size = 50 INFO @ Sun, 02 Jun 2019 22:43:53: #1 total tags in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:53: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 22:43:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 22:43:53: #1 tags after filtering in treatment: 28357693 INFO @ Sun, 02 Jun 2019 22:43:53: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 22:43:53: #1 finished! INFO @ Sun, 02 Jun 2019 22:43:53: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 22:43:53: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 22:43:55: #2 number of paired peaks: 59 WARNING @ Sun, 02 Jun 2019 22:43:55: Too few paired peaks (59) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sun, 02 Jun 2019 22:43:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/ce10/SRX5402707/SRX5402707.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。