Job ID = 1293035 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,103,851 reads read : 12,207,702 reads written : 6,103,851 reads 0-length : 6,103,851 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:26 6103851 reads; of these: 6103851 (100.00%) were unpaired; of these: 337722 (5.53%) aligned 0 times 4826307 (79.07%) aligned exactly 1 time 939822 (15.40%) aligned >1 times 94.47% overall alignment rate Time searching: 00:02:26 Overall time: 00:02:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 7 sequences. [bam_rmdupse_core] 276852 / 5766129 = 0.0480 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 21:33:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:33:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:33:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:33:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:33:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:33:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:33:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam -f BAM -g ce -n /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.bam'] # control file = None # effective genome size = 9.00e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 21:33:17: #1 read tag files... INFO @ Sun, 02 Jun 2019 21:33:17: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 21:33:27: 1000000 INFO @ Sun, 02 Jun 2019 21:33:28: 1000000 INFO @ Sun, 02 Jun 2019 21:33:29: 1000000 INFO @ Sun, 02 Jun 2019 21:33:37: 2000000 INFO @ Sun, 02 Jun 2019 21:33:37: 2000000 INFO @ Sun, 02 Jun 2019 21:33:40: 2000000 INFO @ Sun, 02 Jun 2019 21:33:46: 3000000 INFO @ Sun, 02 Jun 2019 21:33:46: 3000000 INFO @ Sun, 02 Jun 2019 21:33:50: 3000000 INFO @ Sun, 02 Jun 2019 21:33:55: 4000000 INFO @ Sun, 02 Jun 2019 21:33:56: 4000000 INFO @ Sun, 02 Jun 2019 21:34:01: 4000000 INFO @ Sun, 02 Jun 2019 21:34:04: 5000000 INFO @ Sun, 02 Jun 2019 21:34:05: 5000000 INFO @ Sun, 02 Jun 2019 21:34:09: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 21:34:09: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 21:34:09: #1 total tags in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:09: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:34:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:34:09: #1 tags after filtering in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:34:09: #1 finished! INFO @ Sun, 02 Jun 2019 21:34:09: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:34:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:34:09: #2 number of paired peaks: 361 WARNING @ Sun, 02 Jun 2019 21:34:09: Fewer paired peaks (361) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 361 pairs to build model! INFO @ Sun, 02 Jun 2019 21:34:09: start model_add_line... INFO @ Sun, 02 Jun 2019 21:34:09: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 21:34:09: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 21:34:09: #1 total tags in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:09: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:34:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:34:09: start X-correlation... INFO @ Sun, 02 Jun 2019 21:34:09: end of X-cor INFO @ Sun, 02 Jun 2019 21:34:09: #2 finished! INFO @ Sun, 02 Jun 2019 21:34:09: #2 predicted fragment length is 75 bps INFO @ Sun, 02 Jun 2019 21:34:09: #2 alternative fragment length(s) may be 75 bps INFO @ Sun, 02 Jun 2019 21:34:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10_model.r WARNING @ Sun, 02 Jun 2019 21:34:09: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:34:09: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sun, 02 Jun 2019 21:34:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:34:09: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:34:09: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:34:09: #1 tags after filtering in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:09: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:34:09: #1 finished! INFO @ Sun, 02 Jun 2019 21:34:09: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:34:09: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:34:10: #2 number of paired peaks: 361 WARNING @ Sun, 02 Jun 2019 21:34:10: Fewer paired peaks (361) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 361 pairs to build model! INFO @ Sun, 02 Jun 2019 21:34:10: start model_add_line... INFO @ Sun, 02 Jun 2019 21:34:10: start X-correlation... INFO @ Sun, 02 Jun 2019 21:34:10: end of X-cor INFO @ Sun, 02 Jun 2019 21:34:10: #2 finished! INFO @ Sun, 02 Jun 2019 21:34:10: #2 predicted fragment length is 75 bps INFO @ Sun, 02 Jun 2019 21:34:10: #2 alternative fragment length(s) may be 75 bps INFO @ Sun, 02 Jun 2019 21:34:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20_model.r WARNING @ Sun, 02 Jun 2019 21:34:10: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:34:10: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sun, 02 Jun 2019 21:34:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:34:10: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:34:10: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:34:11: 5000000 INFO @ Sun, 02 Jun 2019 21:34:16: #1 tag size is determined as 76 bps INFO @ Sun, 02 Jun 2019 21:34:16: #1 tag size = 76 INFO @ Sun, 02 Jun 2019 21:34:16: #1 total tags in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:16: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 21:34:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 21:34:16: #1 tags after filtering in treatment: 5489277 INFO @ Sun, 02 Jun 2019 21:34:16: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 21:34:16: #1 finished! INFO @ Sun, 02 Jun 2019 21:34:16: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 21:34:16: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 21:34:17: #2 number of paired peaks: 361 WARNING @ Sun, 02 Jun 2019 21:34:17: Fewer paired peaks (361) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 361 pairs to build model! INFO @ Sun, 02 Jun 2019 21:34:17: start model_add_line... INFO @ Sun, 02 Jun 2019 21:34:17: start X-correlation... INFO @ Sun, 02 Jun 2019 21:34:17: end of X-cor INFO @ Sun, 02 Jun 2019 21:34:17: #2 finished! INFO @ Sun, 02 Jun 2019 21:34:17: #2 predicted fragment length is 75 bps INFO @ Sun, 02 Jun 2019 21:34:17: #2 alternative fragment length(s) may be 75 bps INFO @ Sun, 02 Jun 2019 21:34:17: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05_model.r WARNING @ Sun, 02 Jun 2019 21:34:17: #2 Since the d (75) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sun, 02 Jun 2019 21:34:17: #2 You may need to consider one of the other alternative d(s): 75 WARNING @ Sun, 02 Jun 2019 21:34:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sun, 02 Jun 2019 21:34:17: #3 Call peaks... INFO @ Sun, 02 Jun 2019 21:34:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 21:34:26: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:34:26: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:34:33: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 21:34:33: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10_peaks.xls INFO @ Sun, 02 Jun 2019 21:34:33: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:34:33: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.10_summits.bed INFO @ Sun, 02 Jun 2019 21:34:33: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (313 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:34:34: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20_peaks.xls INFO @ Sun, 02 Jun 2019 21:34:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:34:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.20_summits.bed INFO @ Sun, 02 Jun 2019 21:34:34: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (175 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 21:34:41: #4 Write output xls file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05_peaks.xls INFO @ Sun, 02 Jun 2019 21:34:41: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 21:34:41: #4 Write summits bed file... /home/okishinya/chipatlas/results/ce10/SRX5020801/SRX5020801.05_summits.bed INFO @ Sun, 02 Jun 2019 21:34:41: Done! pass1 - making usageList (7 chroms): 1 millis pass2 - checking and writing primary data (453 records, 4 fields): 6 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。